Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients by Nasser M. Al-Daghri, Andrea S. Costa, Majed S. Alokail, Milena Zanzottera, Amal M. Alenad, Abdul Khader Mohammed, Mario Clerici, Franca R. Guerini

“…A possible role of Snap25 polymorphisms in type 2 diabetes mellitus (T2DM) was evaluated by analyzing three SNPs within intron 1 in a region known to affect the gene expression in vitro. Genomic DNA from 1019 Saudi individuals (489 confirmed T2DM and 530 controls) was genotyped for SNPs rs363039, rs363043, and rs363050 in Snap25 using the TaqMan Genotyping Assay. …”
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Insights into microbial compositions of the respiratory tract of neonatal dairy calves in a longitudinal probiotic trial through 16S rRNA sequencing by Jia W. Tan, Susan D. Eicher, Janice E. Kritchevsky, Keith A. Bryan, Aaron Dickey, Carol G. Chitko-McKown, Tara G. McDaneld

“…While studies have characterized the calf lung bacterial profile with and without oral probiotics, simultaneous probiotic effects on the bacterial populations of multiple sites along the respiratory tract have not been characterized.MethodsThis study utilized the same pre-weaning diary calf group from our previous studies to characterize the bacterial populations present in the nostril and tonsil across control and treatment groups and nine sampling time points. DNA was exacted from the nostril and tonsil swabs and lung lavage fluids, and 16S ribosomal RNA gene hypervariable regions 1-3 were subsequently sequenced.ResultsTemporal variation in alpha bacterial diversity within the nostril, tonsil, and lung samples was observed, indicating distinct bacterial compositions among sampling time points. …”
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Advancements in Managing Anthracycline-Induced Cardiotoxicity: Insights from Interventional Clinical Trials by Zhao M, Zhang X, Zhou D, Song J

“…Advanced monitoring techniques, including global longitudinal strain (GLS)-guided echocardiography, cardiac magnetic resonance (CMR) and novel biomarkers (cfDNA, microRNA), showed promise in early AIC detection. …”
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Gut microbial alteration in chronic spontaneous urticaria unresponsive to second generation antihistamines and its correlation with disease characteristics‐ a cross‐sectional case‐... by Indrashis Podder, David Pesqué, Nerea Carrón, Pedro Iñaki González Torres, Ramon M. Pujol, Ana M. Giménez‐Arnau

“…Clinico‐demographic profile, laboratory investigations and stool analysis were conducted in all study participants. 16S RNA gene sequencing and DNA isolation was performed for all stool samples, followed by bioinformatic analysis. …”
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利用Illumina MiSeq测序平台分析健康与腹泻食蟹猴粪样菌群 by 张飞燕 1 金洁 1 刘超 1 胡正飞 1 张玉林 1 谢丽分 1 邬继文 1 吕龙宝 1

“…采集成年腹泻与健康食蟹猴粪样各6份，用细菌16Sr DNA通用引物扩增V3—V4 区，采用Illumina MiSeq测序平台对食蟹猴粪便微生物进行研究。…”
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Morphotypes and genetic diversity of <i>Dendrobaena schmidti</i> (Lumbricidae, Annelida) by S. V. Shekhovtsov, I. B. Rapoport, T. V. Poluboyarova, A. P. Geraskina, E. V. Golovanova, S. E. Peltek

“…We studied the genetic diversity of D. schmidti from seven locations from the Western Caucasus using mitochondrial (a fragment of the cytochrome oxidase I gene) and nuclear (internal ribosomal transcribed spacer 2) DNA. For both genes studied, we found that our sample was split into two groups. …”
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Discovery of novel PRMT1 inhibitors: a combined approach using AI classification model and traditional virtual screening by Jungan Zhang, Yixin Ren, Yixin Ren, Yun Teng, Han Wu, Jingsu Xue, Lulu Chen, Xiaoyue Song, Yan Li, Ying Zhou, Zongran Pang, Zongran Pang, Hao Wang, Hao Wang, Hao Wang

“…Protein arginine methyltransferases (PRMTs) play crucial roles in gene regulation, signal transduction, mRNA splicing, DNA repair, cell differentiation, and embryonic development. …”
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广州市儿童致咽炎β溶血性链球菌的流行情况及其emm基因分型

“…【目的】 了解近期广州市儿童致咽炎β溶血性链球菌(BHS)的流行情况及其emm基因分型【方法】 对我院儿科门急诊1 190例急性咽扁桃体炎的患儿进行咽拭子培养鉴定为BHS者包括ABC和G组溶血性链球菌(group ABCG streptococcal,GASGBSGCS和GGS)进行细菌组DNA的提取;通过PCR扩增emm基因并测序;其结果与NCBI和CDC Home中已知的基因序列进行同源性比对大于95%则认为是同型菌株,藉此确定其分型【结果】 在1 190例符合条件的患儿咽拭子标本中[男:678例,(6.96±2.73)岁;女:512例,(6.70±2.60)岁],鉴定为BHS的共有46例(3.87%)其中GASGBSGCS和GGS分别有4例20例2例和20例,分别占BHS 约8.7%43.5%4.3%和43.5%;而GASGBSGCS和GGS的阳性率分别约为0.37%1.68%0.17%和1.68%男患儿BHS的阳性率约为5.01%,女患儿约为2.34 %,两组比较(χ2 = 26.431,P < 0.001)有统计学意义除了GBS外,其他BHS(GASGC/GS)均有emm基因表达(1 000 ~ 1 500 bp)经测序共有15型emm,其中4株GAS分别表达emm 1和emm 12(7.69%)【结论】 致咽炎GAS阳性率低于0.5%,而GBSGGS阳性率接近2%但GBS无emm表达,GAS表达emm 1和emm 12,而GCSGGS无明确的emm优势型…”
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The Russian Brassicaceae collection – from N.I. Vavilov and E.N. Sinskaya till nowadays by A. M. Artemyeva, A. G. Dubovskaya, N. G. Kon’kova

“…Genetic studies of the Brassicaceae collections at VIR include DNA analysis to search for duplicates in the collections, to compare original seeds  and the seeds  after reproduction and to assess the authenticity of saved accessions, to assess biodiversity, including that of new material from collecting missions, to develop phylogenetic studies. …”
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Whole exome sequencing enables the correct diagnosis of Frank–Ter Haar syndrome in a Saudi family by Y. N. Khan, M. Imad A.M. Mahmud, N. Othman, H. M. Radzuan, S. Basit

“…Family pedigree shows an autosomal recessive mode of inheritance. DNA was extracted from the blood samples of six members of the family. …”
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<i>Bacillus anthracis</i> strain differentiation based on SNP and VNTR loci by E. A. Anisimova, N. A. Fakhrutdinov, D. A. Mirgazov, E. A. Dodonova, I. A. Elizarova, M. E. Gorbunova, N. I. Khammadov, L. I. Zainullin, K. A. Osyanin

“…Modern methods of molecular biology make it possible to detect a number of genetic markers suitable for indicating and differentiating the strains of B. anthracis, including the loci arranged as variable number tandem repeats (VNTRs) and SNPs, one nucleotide-sized differences in the DNA sequence of the loci being compared. The objective of the present study was to examine the effectiveness of SNP analysis and PCR amplif ication of VNTR loci combined with the high-resolution amplicon melting analysis for identif ication and differentiation of the anthrax agent strains. …”
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Cadophora species from marine glaciers in the Qinghai-Tibet Plateau: an example of unsuspected hidden biodiversity by Bingqian Zhang, Xiaoguang Li, Guojie Li, Qi-Ming Wang, Manman Wang

“…Two phylogenetic trees were constructed: one was based on the partial large subunit nrDNA (LSU) to infer taxonomic placement of our isolates and the other was based on multi-locus sequences of LSU, ITS, TUB and TEF-1α to investigate more exact phylogenetic relationships between Cadophora and allied genera. …”
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Biomonitoring PhIP, a Potential Prostatic Carcinogen, in the Hair of Healthy Men of African and European Ancestry by Robert J. Turesky, Clarence Jones, Jingshu Guo, Kari Cammerrer, Laura A. Maertens, Emmanuel S. Antonarakis, Zhanni Lu, Logan G. Spector

“…Frequent consumption of well-done meats containing 2-amino-1-methyl-6-phenylimidazo[4,5-<i>b</i>]pyridine (PhIP), a prevalent HAA that is a prostatic carcinogen in rodents and DNA-damaging agent in human prostate cells, has been linked to aggressive prostate cancer (PC) pathology. …”
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Advances in chromosomal microarray analysis: Transforming neurology and neurosurgery by Wireko Andrew Awuah, Muhammad Hamza Shah, Vivek Sanker, Krishitha Meenu Mannan, Sruthi Ranganathan, Princess Afia Nkrumah-Boateng, Mabel Frimpong, Kwadwo Darko, Joecelyn Kirani Tan, Toufik Abdul-Rahman, Oday Atallah

“…Offering unparalleled sensitivity, CMA detects submicroscopic chromosomal imbalances, enabling the examination of DNA for copy number variations, deletions, duplications, and other structural differences. …”
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重组人IL-6对近曲肾小管上皮细胞增殖及生物学表型的影响 by 詹林达, 梁蔚文, 魏菁, 邹和群

“…【结果】MTT比色法示，rIL-6在10～50ng／mL作用浓度范围内促进HK-2细胞增殖，呈剂量、时间依赖效应；rIL-6作用浓度为100～200ng／mL时抑制细胞增殖，随作用浓度增加抑制作用加强（F=201．582，P〈0．001），（F=43．943，P〈0．001）。rIL-6影响HK-2细胞的DNA合成。HK-2细胞有基础量的α—SMA表达，25ng／mL rIL-6与HK-2孵育1～48h后，α—SMA阳性细胞百分率高峰出现在48h（F=442．22，P〈0．001）。…”
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Prevalence of hepatitis B virus and associated factors among blood donors in Hossana blood bank catchment area, Southern Ethiopia by Mesfin Menza Jaldo, Mhireteab WoldeYohannes Joffe, Eyasu Samuel Zemedkun

“…Abstract Background Human hepatitis is an inflammation of the liver brought on by the DNA virus known as the hepatitis B virus (HBV). …”
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The Nrf-2/HO-1 Signaling Axis: A Ray of Hope in Cardiovascular Diseases by Xueyan Zhang, Yihan Yu, Hanyu Lei, Yufeng Cai, Jie Shen, Ping Zhu, Qingnan He, Mingyi Zhao

“…It is activated, transferred to the nucleus, and bound to DNA by antioxidant response elements (AREs). As a regulator of the antioxidant system, it upregulates the expression of HO-1 to reduce oxidative stress. …”
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Nuclear and chloroplast genome variability in leek (Allium porrum L.) by E. A. Dyachenko, T. M. Seredin, M. A. Filyushin

“…As a result of an AFLP analysis and processing of the DNA spectra obtained, 760 fragments were identified, of which 716 were polymorphic for the leek accessions being analyzed. …”
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Spatial genetic characterization of the red fox (Vulpes vulpes) in the area between the Alps and the Central Dinaric Mountains by A. Devedžić, F. F. Urzi, B. Pokorny, G. Vengušt, D. Ž. Vengušt, F. Janžekovič, L. Velić, T. Eterović, B. K. Stroil, E. Bužan

“…We successfully isolated DNA and genotyped 118 red fox samples. Our analyses, which included Bayesian clustering techniques, revealed a weak genetic differentiation among the studied populations. …”
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Differential infectivity by the oral route of Trypanosoma cruzi lineages derived from Y strain. by Cristian Cortez, Rafael M Martins, Renan M Alves, Richard C Silva, Luciana C Bilches, Silene Macedo, Vanessa D Atayde, Silvia Y Kawashita, Marcelo R S Briones, Nobuko Yoshida

“…Network genealogies of four nuclear genes (SSU rDNA, actin, DHFR-TS, EF1α) revealed that Y30 is closely related to Discrete Typing Unit TcII while Y82 is more closely related to TcVI, a group containing hybrid strains. …”
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