Establishment of a Mutant Library for Infection Cushion Development and Identification of a Key Regulatory Gene in <i>Botrytis cinerea</i> by Maoyao Tang, Kexin Wang, Pan Zhang, Jie Hou, Xiaoqian Yu, Hongfu Wang, Yangyizhou Wang, Guihua Li

“…In this study, we utilized a forward genetics approach to establish a large T-DNA tagged population of <i>B. cinerea</i>, which contained 14,000 transformants. …”
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Emerging of Fatal Colitis with Multidrug-Resistant Candida glabrata after Small Bowel Transplantation by Zahra Zareshahrabadi, Mojtaba Shafiekhani, Hamed Nikoupour, Hasti Nouraei, Hamid Morovati, Kamiar Zomorodian

“…After detection of budding yeast in the stool samples, stool culture was positive for Candida, DNA was extracted, and ITS1-5.8s-ITS2 region of the fungal agent was amplified. …”
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Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan by Babayeva Sevda, Shikhaliyeva Kamila, Qurbanova Qamar, Hajiyeva Sabina, Izzatullayeva Vusala, Rustamov Khanbala, Abbasov Mehraj

“…The aim of this study was to assess the genetic diversity of 50 lentil accessions, including landraces from high mountain regions of Azerbaijan as well as wild lentil species, using SSR markers. DNA was extracted from leaves, amplified by PCR and separated by polyacrylamide gel electrophoresis. …”
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Noncanonical IFN Signaling, Steroids, and STATs: A Probable Role of V-ATPase by Howard M. Johnson, Ezra Noon-Song, Chulbul M. Ahmed

“…Activated JAKs such as JAK2 and TYK2 are also involved in the epigenetics of nucleosome unwrapping for exposure of DNA to transcription. Furthermore, activated JAKs and STATs appear to function coordinately for specific gene activation. …”
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High Prevalence of Human Papillomavirus Infection among Brazilian Pregnant Women with and without Human Immunodeficiency Virus Type 1 by Emilia Moreira Jalil, Geraldo Duarte, Patrícia El Beitune, Renata Toscano Simões, Patrícia Pereira dos Santos Melli, Silvana Maria Quintana

“…Cervicovaginal specimens were obtained from all women during gynecologic exam. HPV DNA, low and high risk HPV types, was detected using conventional PCR. …”
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Diversity of <i>mariner</i>-like elements in Orthoptera by K. Ustyantsev, M. Biryukov, I. Sukhikh, N. V. Shatskaya, V. Fet, A. Blinov, I. Konopatskaia

“…Mariner-like elements (MLEs) are among the most widespread DNA transposable elements in eukaryotes. Insects were the first organisms in which MLEs were identified, however the diversity of MLEs in the insect order Orthoptera has not yet been addressed. …”
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Telomere length varies with sex, hatching rank and year of birth in the Little Owl, Athene noctua by Criscuolo, François, Fache, Inès, Scaar, Bertrand, Zahn, Sandrine, Bleu, Josefa

“…Telomeres are non-coding DNA sequences located at the end of linear chromosomes, protecting genome integrity. …”
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Role of sirtuins in epigenetic regulation and aging control by E. M. Samoilova, S. E. Romanov, D.  A. Chudakova, P. P. Laktionov

“…This multifactorial regulatory system affects many processes: cellular metabolism, mitochondrial functions, epigenetic regulation, DNA repair and more. As is expected, the activity of sirtuin proteins affects the manifestation of classic signs of aging in the body, such as cellular senescence, metabolic disorders, mitochondrial dysfunction, genomic instability, and the disruption of epigenetic regulation. …”
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Neolithic pastoralism and plant community interactions at high altitudes of the Pyrenees, southern Europe by Irene Julián-Posada, Graciela Gil-Romera, Sandra Garcés-Pastor, Peter D. Heintzman, Daniel Gómez, Federico Fillat, Ana Moreno, Javier Lara-Recuero, Pere Bover, Lourdes Montes, Alejandro Sierra, Blas Valero-Garcés, Inger G. Alsos, Penélope González-Sampériz

“…We generated a comprehensive sedimentary ancient DNA record from the central Pyrenees, spanning 12,200 to 1300 years before present, revealing the earliest continuous presence of sheep (6500 years before present) and cattle (5900 years before present) in alpine southern Europe. …”
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Transcriptional activity of ammonia oxidisers in response to soil temperature, moisture and nitrogen amendment by Chris Chisholm, Hong Di, Keith Cameron, Andriy Podolyan, Jupei Shen, Limei Zhang, Kosala Sirisena, Xueying Che

“…Peak AOB amoA transcript abundance was positively correlated with estimated soil ammonia availability. While AOB gDNA abundance and nitrification rate trends were similar. …”
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Dickkopf-1 Is a Biomarker for Systemic Lupus Erythematosus and Active Lupus Nephritis by Jing Xue, Jiali Yang, Lijuan Yang, Shaolan Zhou, Chen Ji, Xuemei Wang, Nan Yu, Xiaoming Liu, Shuhong Chi

“…Moreover, a combination of DKK-1 with anti-dsDNA and/or levels of complement C3 and C4 could not increase the specificity and/or sensitivity for identification of patients with LN diseases, but both ROC curve and multiple-factor nonconditional logistic regression analysis showed that serum DKK-1 was considered better positive biomarker for identification of LN in SLE patients. …”
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SPD_0410 negatively regulates capsule polysaccharide synthesis and virulence in Streptococcus pneumoniae D39 by Ye Tao, Li Lei, Shuhui Wang, Xuemei Zhang, Yibing Yin, Yuqiang Zheng

“…In this study, we used electrophoretic mobility shift assays and DNA enzyme footprinting to identified the hypothetical protein SPD_0410 as a negative regulator of cps locus. …”
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Exceptional Enlargement of the Mitochondrial Genome Results from Distinct Causes in Different Rain Frogs (Anura: Brevicipitidae: Breviceps) by Keitaro Hemmi, Ryosuke Kakehashi, Chiaki Kambayashi, Louis Du Preez, Leslie Minter, Nobuaki Furuno, Atsushi Kurabayashi

“…In contrast, the B. poweri mtDNA (28.1 kbp) is considerably larger: currently the second largest among vertebrates, after B. adspersus. …”
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A descriptive-multivariate analysis of community knowledge, confidence, and trust in COVID-19 clinical trials amongst Ugandans working in healthcare settings. by Keneth Iceland, Kasozi, Lawrence Obado, Osuwat

“…KCTCOVacTrials were higher amongst the least educated (certificate holders) than bachelor degree holders. Skepticism against DNA recombinant vaccines (DRV) implies genetically modified vaccines such as the Oxford- AstraZeneca vaccine, Pfizer/BioNTech are bound to face a level of resistance once adapted in Uganda. …”
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Emerging Anthelmintic Resistance in Poultry: Can Ethnopharmacological Approaches Offer a Solution? by Zirintunda, Gerald, Biryomumaisho, Savino, Keneth Iceland, Kasozi, Batiha, Gaber El-Saber, Kateregga, John, Vudriko, Patrick, Nalule, Sarah, Olila, Deogracious, Mariam Kajoba, Mariam, Matama, Kevin, Kwizera, Mercy Rukundo, Ghoneim, Mohammed M., Abdelhamid, Mahmoud, Alshehri, Sultan, Abdelgawad, Mohamed A., Acai-Okwee, James, Zaghlool, Sameh S.

“…Benzyl isothiocyanate glucosinolates interfere with DNA replication and protein expression, while isoflavones from Acacia oxyphylla cause helminth flaccid paralysis, inhibit energy generation, and affect calcium utilization. …”
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همسانه‌سازی، توالی‌یابی و بررسی بیان ژن C4H در آنغوزه تلخ Ferula pseudalliacea by پگاه شهیدی, یاور وفایی, بهمن بهرام نژاد, دارا دستان

“…استخراج RNA از بافت های مختلف گیاه Ferula pseudalliacea با روش لیتیم کلرید انجام گرفت. جهت سنتز cDNA ، از کیت سنتز cDNA شرکت یکتا تجهیز در حجم 20 میکرولیتر استفاده شد. …”
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Evaluation of dried blood spot sampling for real-time PCR malaria diagnostics in a rural setting in Angola by Alejandro Mediavilla, Begoña Febrer-Sendra, Aroa Silgado, Patricia Martínez-Vallejo, Beatriz Crego-Vicente, Arlette Nindia, Carles Rubio Maturana, Lidia Goterris, Joan Martínez-Campreciós, Sandra Aixut, Pedro Fernández-Soto, María Luisa Aznar, Antonio Muro, Inés Oliveira-Souto, Israel Molina, Elena Sulleiro

“…Conclusions The DBS volume used for DNA extraction is a determining factor in the performance of real-time PCR for Plasmodium DNA detection. …”
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Whole genomic analysis uncovers high genetic diversity of rifampicin-resistant Mycobacterium tuberculosis strains in Botswana by Tuelo Mogashoa, Tuelo Mogashoa, Johannes Loubser, Ontlametse T. Choga, Ontlametse T. Choga, Justice Tresor Ngom, Wonderful T. Choga, Wonderful T. Choga, Mpaphi B. Mbulawa, Tuduetso Molefi, One Stephen, Topo Makhondo, Kedumetse Seru, Patience Motshosi, Boitumelo Zuze, Joseph Makhema, Rosemary M. Musonda, Dimpho Otukile, Chawangwa Modongo, Botshelo T. Kgwaadira, Keabetswe Fane, Simani Gaseitsiwe, Rob M. Warren, Sikhulile Moyo, Sikhulile Moyo, Sikhulile Moyo, Sikhulile Moyo, Anzaan Dippenaar, Elizabeth M. Streicher

“…Library preparation was performed using the Illumina DNA prep kit following the manufacturer's instructions. …”
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Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway by Yan Li, Hong Wang, Xiaoyang Li, Yuan He, Huiquan Liu, Dong Han, Ting Mei, Yixuan Li, Zilong Xu, Xiaoying Wei, Jianguo Wang, Peijie Zhou, Yuwen Wang, Ziye Li, Junhao Ning, Anlin Wang, Jingjing Cheng, Dong Qian

“…Tumor cells intrinsic mitochondria dynamics could pass effects on the extracellular microenvironment through mtDNA stress. PGAM5 anchors at mitochondria and regulates mitochondria functions. …”
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In Vitro Antibacterial, Antioxidant, Cytotoxicity Activity, and In Silico Molecular Modelling of Compounds Isolated from Roots of Hydnora johannis by Teshome Degfie, Milkyas Endale, Muhdin Aliye, Rajalakshmanan Eswaramoorthy, Tariku Nefo Duke, Aman Dekebo

“…In each docking pose, catechin (3) scored higher binding affinity of −7.9, −7.2, and −6.4 kcal/mol towards PqsA, DNA gyraseB, and S. aureus PK, respectively, compared to amoxicillin (−8.1, −6.1, and −6.4 kcal/mol). …”
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