Status of the Multidrug Resistance-1 Gene of Plasmodium falciparum in Four Malaria Epidemiological Strata, Two Decades after the Abolition of Chloroquine as First-Line Treatment fo... by David Denis Sofeu-Feugaing, Fabrice Nkengeh Ajonglefac, Marcel Nyuylam Moyeh, Tobias Obejum Apinjoh, Marianne Elodie Essende, Gilchrist Destin Talla Kouam, Stephen Mbigha Ghogomu

“…Malaria-positive blood samples were collected from 798 patients on Whatman filter paper to determine the status of resistance to CQ. DNA was extracted by boiling in Chelex and analysis of Plasmodium species. …”
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The Sequence of Prostaglandin-Endoperoxide Synthase 2 Gene in Women with Cervicitis by Maha A. Ahlial, Nazar Sh. Mohammed, Maysara S. Khalaf

“…The conventional PCR was used for gene detection and DNA sequencing. The mean± SD of Ca125, CA15.3 and CRP showed a highly significant differences between patient (30.33±1.09), (22.82±1.07), (88.06±5.88) respectively and the healthy control (19.40±0.68), (18.34±0.77), (6.73±0.12) respectively-value >0.001. …”
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Development and application of decontamination methods for the re-use of laboratory grade plastic pipette tips. by Sang Hyuk Lee, William Kastor, Xiao Fu, Vikas Soni, Michael Keidar, Marc Donohue, Steve Wood, Enusha Karunasena

“…All tips (control and experimental groups) were introduced to the methods described, while tips exposed to DNA extracts of Aeromonas hydrophila (ATCC-23211) were assessed for experimental groups. …”
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Variations in Semen Quality and Potential for Frozen Semen Production in Aceh Cattle by E. Sophian, S. Said, M. A. Setiadi, R. I. Arifiantini

“…The highest progressive motility was observed in bulls 211710 and 211605, and the highest intact DNA was detected in bulls 211710 and 211605. The results of this study demonstrated that the quality of fresh and frozen semen in Aceh cattle is distinct, and the potential frozen semen production of Aceh cattle is estimated to range from 3,382 ± 1810 to 11,399 ± 2658 straws/year. …”
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新型脱细胞脑组织修复支架的制备及其细胞相容性

“…【方法】取成年Sprague-Dawley （SD）大鼠脑皮质，运用冻融、Triton X-100、脱氧胆酸钠、DNA/RNA酶等进行脱细胞处理并予京尼平（Genipin,GP）交联，对处理后的支架材料进行形态结构观察、主要成分分析和细胞相容性观察。…”
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Antiglycating effects of citrus flavonoids and associated mechanisms by Yunli Xiao, Junfeng Shen, Jianfeng Zhan, Limin Guo, Chi-Tang Ho, Shiming Li

“…Glycation of proteins and DNA forms advanced glycation end products (AGEs) causing cell and tissue dysfunction and subsequent various chronic diseases, in particular, metabolic and age-related diseases. …”
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Modern issues of sugar beet (<i>Beta vulgaris</i> L.) hybrid breeding by S. D. Karakotov, I. V. Apasov, A. A. Nalbandyan, E. N. Vasilchenko, T. P. Fedulova

“…It has been found that DNA markers of the sugar beet mitochondrial genome belonging to the TR minisatellite family (TR1 and TR3) enable reliable enough identification of haploid microclonal plants as MSor O-type forms. …”
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Vitamin D Receptor Polymorphisms Predispose to Primary Biliary Cirrhosis and Severity of the Disease in Polish Population by Agnieszka Kempińska-Podhorecka, Ewa Wunsch, Tomasz Jarowicz, Joanna Raszeja-Wyszomirska, Beata Loniewska, Mariusz Kaczmarczyk, Małgorzata Milkiewicz, Piotr Milkiewicz

“…Control group comprises 306 DNA samples from umbilical cord blood of healthy newborn children. …”
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Novel Pt (II) Complexes With Anticancer Activity Against Pancreatic Ductal Adenocarcinoma Cells by Erika Stefàno, Gianluca Rovito, Luca G. Cossa, Federica De Castro, Viviana Vergaro, Asjad Ali, Giulia My, Danilo Migoni, Antonella Muscella, Santo Marsigliante, Michele Benedetti, Francesco Paolo Fanizzi

“…Platinum-based drugs containing the bidentate ligand phenanthroline have been found to have strong antitumor activity due to their ability to cause DNA damage. In this study, we examined the ability of two Pt (II) cationic complexes, [Pt(η1-C2H4OR) (DMSO) (phen)]+ (in short Pt-EtORSOphen; R = Me, 1; Et, 2), to inhibit the growth and spread of BxPC-3 PDAC cells, in comparison to CDDP. …”
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Characterizing temporal and global host innate immune responses against SARS-CoV-1 and -2 infection in pathologically relevant human lung epithelial cells. by Vivian Y Tat, Aleksandra K Drelich, Pinghan Huang, Kamil Khanipov, Jason C Hsu, Steven G Widen, Chien-Te Kent Tseng, George Golovko

“…., bone marrow stromal cell antigen 2 (BST2), Z-DNA Binding Protein 1 (ZBP1), C-X-C Motif Chemokine Ligand 11 (CXCL11), and Interferon Induced Transmembrane Protein 1 (IFITM1), were identified as potential drug targets against β-CoVs. …”
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Evaluation of Inflammation-Related Proteins in Multiple Sclerosis Disease with Relapses and Remissions by Nisa Hocaoğlu, Nur Damla Korkmaz, Birsen Elibol, Zehra Cemre Karakayalı, Şule Terzioğlu-Uşak, Azize Esra Gürsoy

“…After RNA isolation and cDNA conversion, NLRP3, ASC, NLRX1, IL-1&#946;, and IL-18 gene expressions were measured in real time-polymerase chain reaction. …”
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Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor by Junjie Zhao, Niu Niu, Fan Yang, Haitao Liu, Weibo Qi

“…Cancer cell proliferation, invasion, and migration were suppressed, and DDP sensitivity was enhanced, causing DNA damage and facilitating apoptosis of DDP‐resistant cells. …”
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大鼠BDNF基因复制缺陷型重组腺病毒的构建与鉴定 by 王鹏, 吴燕峰, 黄文林, 唐勇, 黄霖, 杨睿, 沈慧勇

“…Lipofectamine^TM 2000法转染HEK293细胞包装携带BDNF目的基因的重组腺病毒，少量提取重组腺病毒DNA检测证实为复制缺陷型BDNF基因重组腺病毒后大量扩增。蛋白电泳及Western blot法初步检测BDNF目的基因在HEK293细胞中的表达。…”
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A Case of Systemic Lupus Erythematosus/Antineutrophil Cytoplasmic Antibody-Associated Vasculitis Overlap Syndrome with Dissociated Pathological and Immunological Findings by Kazuhiko Kato, Tetsuya Kawamura, Risa Terashima, Yukiko Tsuchiya, Yasuhito Takahashi, Kenji Kasai, Takashi Yokoo

“…SLE was suspected due to the identification of four items from the diagnostic criteria of the American College of Rheumatology, including positivity for anti-ds-DNA and antinuclear antibodies. However, pathological findings from the kidney biopsy suggested pauci-immune crescentic glomerulonephritis. …”
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Investigation of the Frequency and Characteristic Features of De Novo Mutations in Clinical Exome Sequence Trio Samples by Ebru Marzioğlu-özdemir, Tülin Çora, Ozkan Bagci, Batuhan Şanlıtürk, Ali Torabi, Nadir Koçak

“…Methods: A total of 69 families who underwent Trio Clinical Exome Sequencing (CES) analysis at the Department of Medical Genetics, Faculty of Medicine, Selçuk University, between January 1, 2017, and December 31, 2023, were included in the study. DNA samples extracted from peripheral venous blood of individuals were sequenced using the Roche CES kit and DNBSEQ-G400™ sequencing device, and a total of 3892 genes were analyzed using the Seq Platform. …”
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Structural basis of SIRT7 nucleosome engagement and substrate specificity by Carlos Moreno-Yruela, Babatunde E. Ekundayo, Polina N. Foteva, Dongchun Ni, Esther Calvino-Sanles, Henning Stahlberg, Beat Fierz

“…The catalytic domain binds at the H3-tail exit site, engaging both DNA gyres of the nucleosome. Contacting H3K36 versus H3K18 requires a change in binding pose, and results in structural changes in both SIRT7 and the nucleosome. …”
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SequenceCraft: machine learning-based resource for exploratory analysis of RNA-cleaving deoxyribozymes by M. Eremeyeva, Y. Din, N. Shirokii, N. Serov

“…Abstract Background Deoxyribozymes or DNAzymes represent artificial short DNA sequences bearing many catalytic properties. …”
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γ-H2AX: A Novel Prognostic Marker in a Prognosis Prediction Model of Patients with Early Operable Non-Small Cell Lung Cancer by E. Chatzimichail, D. Matthaios, D. Bouros, P. Karakitsos, K. Romanidis, S. Kakolyris, G. Papashinopoulos, A. Rigas

“…The aim of the current study was to assess the prognostic value of a series of clinical and molecular variables with the addition of γ-H2AX—a new DNA damage response marker—for the prediction of prognosis in patients with early operable non-small cell lung cancer by comparing the γ-H2AX-based artificial network prediction model with the corresponding LR one. …”
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东北林蛙PDK1基因的克隆及生物信息学分析 by 巩珊珊 邵 婕 曲俐俐 柴龙会 张晶钰 肖向红

“…本研究基于东北林蛙转录组数据,从中鉴定出PDK1基因3条Unigene序列,并通过RACE法克隆了PDK1基因的全长cDNA,命名为rdPDK1,其全长为1 990 bp,开放阅读框长1 674 bp,编码557个氨基酸。…”
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Prevalence and methodologies for detection, characterization and subtyping of Listeria monocytogenes and L. ivanovii in foods and environmental sources by Jin-Qiang Chen, Patrick Regan, Pongpan Laksanalamai, Stephanie Healey, Zonglin Hu

“…In this review, we (A) described L. monocytogenes and L. ivanovii, world-wide incidence of listeriosis, and prevalence of various L. monocytogenes strains in food and environmental sources; (B) comprehensively reviewed different types of traditional and newly developed methodologies, including culture-based, antigen/antibody-based, LOOP-mediated isothermal amplification, matrix-assisted laser desorption ionization-time of flight-mass spectrometry, DNA microarray, and genomic sequencing for detection and characterization of L. monocytogenes in foods and environmental sources; (C) comprehensively summarized different subtyping methodologies, including pulsed-field gel electrophoresis, multi-locus sequence typing, ribotyping, and phage-typing, and whole genomic sequencing etc. for subtyping of L. monocytogenes strains from food and environmental sources; and (D) described the applications of these methodologies in detection and subtyping of L. monocytogenes in foods and food processing facilities.…”
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