The master male sex determinant Gdf6Y of the turquoise killifish arose through allelic neofunctionalization by Annekatrin Richter, Hanna Mörl, Maria Thielemann, Markus Kleemann, Raphael Geißen, Robert Schwarz, Carolin Albertz, Philipp Koch, Andreas Petzold, Torsten Kroll, Marco Groth, Nils Hartmann, Amaury Herpin, Christoph Englert

“…Looking for downstream effectors of Gdf6Y, we identified besides TGF-β signaling modulators, especially the inhibitor of DNA binding genes id1/2/3, the mRNA decay activator zfp36l2 as a new GDF6 signaling target.…”
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早期鼻咽癌基因甲基化谱的初步构建

“…【方法】 收集I期(T1N0M0)鼻咽癌石蜡标本及炎性增生性鼻咽组织各4例,分别提取DNA,富集两组样品基因组甲基化片段后采用甲基化芯片(NimbleGen)对两组标本进行高通量测序分析,对杂交信号进行扫描及初步数据处理,分析并筛选出两组标本的差异甲基化基因?…”
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The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material by Vasiliki I. Hatzi, Georgia I. Terzoudi, Christina Paraskevopoulou, Vasilios Makropoulos, Demetrios P. Matthopoulos, Gabriel E. Pantelias

“…The PCC has been successfully used in problems involving cell cycle analysis, diagnosis and prognosis of human leukaemia, assessment of interphase chromosome malformations resulting from exposure to radiation or chemicals, as well as elucidation of the mechanisms underlying the conversion of DNA damage into chromosomal damage. In this report, particular emphasis is given to the advantages of the PCC methodology used as an alternative to conventional metaphase analysis in answering questions in the fields of radiobiology, biological dosimetry, toxicogenetics, clinical cytogenetics and experimental therapeutics.…”
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Diversity and Multigene Phylogeny of the Genus <i>Floccularia</i> (Agaricales, Basidiomycota) by Zai-Wei Ge, Hua Qu, Malka Saba, Tian Gao, Martin Ryberg

“…Based on worldwide sampling and multi-locus DNA sequence data (ITS, LSU, <i>rpb2</i>, <i>tef1</i>), the phylogeny of <i>Floccularia</i> was reconstructed, and the species diversity in Asia was critically studied on the basis of morphology and phylogeny. …”
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Colon Cancer Genetic and Epigenetic Changes, Etiopathogenesis, Risk Factors, Treatment: Literature Review by Gabrielė Ūbaitė, Aurelija Remeikaitė, Justas Žilinskas

“…Our findings emphasize the central role of genetic mutations (particularly in APC, TP53, KRAS and MMR) and epigenetic alterations, such as DNA methylation and histone modification, in the pathogenesis of colorectal cancer. …”
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Comparison of Stemness and Gene Expression between Gingiva and Dental Follicles in Children by Chung-Min Kang, Seong-Oh Kim, Mijeong Jeon, Hyung-Jun Choi, Han-Sung Jung, Jae-Ho Lee

“…Comparative gene expression profiles were collected using cDNA microarray. The expression of development, chemotaxis, mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSs) related genes was assessed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). …”
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无创胚胎植入前非整倍体筛查的诊断有效性分析 by 贾磊, 陈静波, 郭映纯, 何姝婧, 张志强, 苏文龙, 宫晓, 方丛

“…结果TE1、TE2及 BCM/BF与相应的ICM的核型一致性分别为66.7%，87.5%和79.2%（<italic>P</italic>&gt;0.05），任意两组之间的差异没有统计学意义（<italic>P</italic>&gt;0.05）。结论利用囊胚培养液/囊胚腔液中的游离DNA行无创胚胎植入前非整倍体筛查可以获得与同期活检的TE2和ICM相似的诊断有效性。…”
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INFORMATIONAL RESOURCES ON POTATO GERMPLASM COLLECTIONS by D. A. Afonnikov, I. V. Totsky, Z. Stasevski

“…An interesting direction is the provision of services to determine the DNA prints (markers) of potato varieties, involvement of potato growers in the process of operational monitoring of diseases and pests of potatoes. …”
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Comparative mitogenome analysis of two ectomycorrhizal fungi (Paxillus) reveals gene rearrangement, intron dynamics, and phylogeny of basidiomycetes by Qiang Li, Yuanhang Ren, Dabing Xiang, Xiaodong Shi, Jianglin Zhao, Lianxin Peng, Gang Zhao

“…The two mitogenomes of Paxillus involutus and P. rubicundulus comprised circular DNA molecules, with the size of 39,109 bp and 41,061 bp, respectively. …”
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Allelic diversity of genes controlling responses to vernalization and photoperiod among spring bread wheat varieties of diverse geographic origin by Zh. T. Kalybekova

“…Ppd genes determine the response of plants to the length of the day, that is, the timing of flowering and the beginning of heading in plants under different cultivation conditions. The use of diagnostic DNA markers made it possible to analyze the presence of allelic combinations of the Vrn and Ppd genes in local and commercial wheat varieties from Europe, Asia, North and South Americas, Africa and Australia. …”
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Identity and Distribution of <i>Triglops metopias</i> (Teleostei, Cottidae) in the Northwestern Pacific by Artem M. Prokofiev, Ilyas N. Mukhametov, Olga R. Emelianova, Svetlana Yu. Orlova, Alexei M. Orlov

“…Forty-one specimens were included in morphological and molecular analyses, including principal component analysis and DNA barcoding. The detailed morphological description of the Kuril Islands specimens is given. …”
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CTSG is a prognostic marker involved in immune infiltration and inhibits tumor progression though the MAPK signaling pathway in non-small cell lung cancer by Qian Dai, Xufeng Yao, Yanke Zhang, Qian Chai, Xueyi Feng, Hongbin Zhu, Lei Zhao

“…CTSG suppresses the MAPK and EMT signaling pathways in NSCLC cells. Moreover, DNA methylation and histone deacetylation have been identified as crucial mechanisms contributing to the decreased expression of CTSG. …”
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Characterization of Microbiota in Endometrial Fluid and Vaginal Secretions in Infertile Women with Repeated Implantation Failure by Kotaro Kitaya, Yoko Nagai, Wataru Arai, Yoshiyuki Sakuraba, Tomomoto Ishikawa

“…On days 6-8 in the luteal phase of the natural, oocyte-pickup, or hormone replacement cycle, the paired EF and VS samples were obtained separately. Extracted genomic DNA was pyrosequenced for the V4 region of 16S ribosomal RNA using a next-generation sequencer. …”
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microRNA-21对膀胱癌T24细胞增殖的影响 及对VEGF和AP1蛋白的调节

“…【目的】 研究microRNA-21(miR-21)对膀胱癌T24细胞系中血管内皮生长因子(vascular endothelial growth factor, VEGF)和活化因子蛋白-1(activator protein-1, AP1)及mRNA表达的调节作用,探讨miR-21对膀胱癌T24细胞增殖的影响65377; 【方法】 用人工合成的miR-21相应的双链互补DNA片段,插入pSilencerTM 3.1-H1 neo载体表达载体,经测序鉴定后作为microRNA的表达质粒;用脂质体将miR-21高表达质粒转染进T24膀胱癌细胞,经G418筛选获得阳性克隆65377;用RT-PCR技术和Western blot方法分别检测该细胞系中VEGF和AP1的mRNA和蛋白表达情况65377;四甲基偶氮唑蓝(MTT)法检测膀胱癌T24细胞增殖的变化情况65377;【结果】 Western blot结果显示miR-21能够增加VEGF和AP1蛋白的表达,RT-PCR方法分析显示VEGF和AP1 mRNA水平升高65377;MTT显示转染后细胞的生长速率比未转染的细胞明显加快65377;【结论】 miR-21促进T24膀胱癌细胞增殖, 提示可能与VEGF和AP1 的低表达有关65377;…”
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Collapsing glomerulopathy associated with parvovirus B19 and systemic lupus erythematosus in a patient with APOL1 high-risk variant for nephropathy by Thaíza Passaglia Bernardes, Thalita Alvarenga Ferradosa Paula, Gabriel Teixeira Montezuma Sales, Patrícia Varela Calais, Renato Demarchi Foresto, Luiz Antonio Moura, Marcelino de Souza Durão Junior, João Bosco Pesquero, Gianna Mastroianni Kirsztajn

“…SLE was diagnosed and parvovirus B19 DNA was detected in serum samples. Kidney biopsy showed CG without any typical features of lupus nephritis. …”
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The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria by Ebrahim Mohammadi, Fakhredin Karimpour, Ramin Lotfi, Fatemeh Zamani, Abbas Aghaei, Rasoul Nasiri Kalmarzi

“…With the consent of the patients, 5 ml of peripheral blood was taken from each patient for DNA extraction (in a CBC tube) and IgE measurement (in a hemolysis tube without anticoagulant). …”
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Regulation of pattern recognition receptor signaling by palmitoylation by Xiaocui Li, Xiaofang Hu, Hongjuan You, Kuiyang Zheng, Renxian Tang, Fanyun Kong

“…Summary: Pattern recognition receptors (PRRs), consisting of Toll-like receptors, RIG-I-like receptors, cytosolic DNA sensors, and NOD-like receptors, sense exogenous pathogenic molecules and endogenous damage signals to maintain physiological homeostasis. …”
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Uncovering the genetic basis of antiviral polyketide limocrocin biosynthesis through heterologous expression by Sofiia Melnyk, Marc Stierhof, Dmytro Bratiichuk, Franziska Fries, Rolf Müller, Yuriy Rebets, Andriy Luzhetskyy, Bohdan Ostash

“…Abstract Background Streptomyces roseochromogenes NRRL 3504 produces clorobiocin, an aminocoumarin antibiotic that inhibits DNA replication. No other natural products have been isolated from this bacterium so far, despite the presence of a rich repertoire of specialized metabolite biosynthesis gene clusters (smBGCs) within its genome. …”
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Surveillance of pathogenic Leptospira among rodents and small mammals in enzootic areas of plague in Pasuruan Indonesia by Siti Amanah Febriani, Kurnia Ritma Dhanti, Kurniawan Kurniawan, Ristiyanto Ristiyanto, Arief Junaedi, Caecilia Hapsari Ceriapuri Sukowati, Farida Dwi Handayani

“…The study revealed that 7.6% (9/119) of rats in the enzootic areas of plague (Sedaeng, Tosari, Surorowo, Petren, Pakis Bincil, Kutukan) were found to carry Leptospira DNA, indicating a significant difference between infection rates per region (p < 0.0001).  …”
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Protective Effects of BDNF against C-Reactive Protein-Induced Inflammation in Women by Nicole Noren Hooten, Ngozi Ejiogu, Alan B. Zonderman, Michele K. Evans

“…BDNF treatment of cells reduced CRP levels and inhibited CRP-induced DNA damage. Conclusion. We discovered an important relationship between hsCRP, 8-oxodG, and BDNF in women at hsCRP levels >3 mg/L. …”
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