IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM by Bagus Muhammad Ihsan, Widyana Lakshmi Puspita, Linda Triana, Wahdaniah, Khayan, Cecep Dani Sucipto

“…The inspection of microfilariae that is currently being developed uses Polymerase Chain Reaction (PCR) to carry out a unique DNA search technique. Purpose: Identify the type of microfilaria present in filariasis patients using Quantitative PCR High- Resolution Melting (qPCR-HRM) and conventional PCR techniques. …”
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Online Monitoring Oxidative Products and Metabolites of Nicotine by Free Radicals Generation with Fenton Reaction in Tandem Mass Spectrometry by Shih-Shin Liang, Yow-Ling Shiue, Chao-Jen Kuo, Su-Er Guo, Wei-Ting Liao, Eing-Mei Tsai

“…Fenton reaction generates free radicals via ferrous ion (Fe2+) and hydrogen peroxide (H2O2) to oxidize DNA and to degrade proteins in cells. In the EEO method, the oxidative products of nicotine including cotinine, cotinine-N-oxide, trans-3′-hydroxycotinine, nornicotine, norcotinine, 4-oxo-4-(3-pyridyl)-butanoic acid, 4-hydroxy-4-(3-pyridyl)-butanoic acid, and nicotine-N′-oxide were detected by tandem mass spectrometer to simulate the changes of nicotine and its derivatives in a time-dependent manner.…”
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Recent updates of centromere proteins in hepatocellular carcinoma: a review by Zhongyuan Yang, Wenjiao Chen, Yunhui Liu, Yuxin Niu

“…Centromeres are considered special deoxyribonucleic acid (DNA) sequences with highly repetitive sequences that are physically connected to the spindle during cell division, ensuring equal division of genetic material between daughter cells. …”
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FBP1 Is an Interacting Partner of Menin by Shadia Zaman, Karen Sukhodolets, Patricia Wang, Jun Qin, David Levens, Sunita K. Agarwal, Stephen J. Marx

“…Menin interacts with proteins that regulate transcription, DNA repair and processing, and maintenance of cytoskeletal structure. …”
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Molecular Characterization of the CYTB Gene of Radix auricularia Linnaeus, 1758 (Mollusca, Gastropoda, Lymnaeidae) in Al-Chibayish of Thi-Qar Province, Southern Iraq by Sarmad A. M. Al-Asadi

“…From November 2023 to May 2024, R. auricularia snails (480) were collected from six stations (A to F) in the Euphrates River in Al-Chibayish and had morphometric measurement variations. DNA was extracted from the station snails and the partial CYTB gene was amplified. …”
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Exposure of Mycobacteriodes abscessus clones to mucin affects bacterial phenotype by Amy Leestemaker-Palmer, Tiffany Ong, Luiz E Bermudez

“…Some of the strains increased the amount of carbohydrates in the biofilm matrix overtime while others exported more DNA, the matrix containing large amounts of it. …”
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Liver Microbiome in Healthy Rats: The Hidden Inhabitants of Hepatocytes by Xiao Wei Sun, Hua Zhang, Xiao Zhang, Peng Fei Xin, Xue Gao, Hong Rui Li, Cai Yun Zhou, Wen Min Gao, Xuan Xuan Kou, Jian Gang Zhang

“…After the rats were sacrificed by blood drainage from the portal vein, we extracted total deoxyribonucleic acid (DNA) from their ileal and colonic contents and liver tissues. …”
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Two cellular models for analyzing mitochondrial heteroplasmy by Yaning Hu, Lin Jiang, Wen Liu, Xingbo Zhao

“…Heteroplasmy is commonly observed in eukaryotes due to mitochondrial genome (mtDNA) mutation, paternal leakage, mitochondria transplantation/mitotherapy, and somatic cell nuclear transfer (SCNT). …”
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A Review: CRISPR Cas System and the Mechanism With an Inhibition of Binding of CRISPR Cas‐9 by Akram Hosen, Md Nakibul Hasan Nishat, MD. Mohaimin Hossain Soaib, Omar Sharif Sharkar, Md. Sahabuddin, Imdadul Haque Sharif, Sujay Kumar Bhajan

“…This review emphasizes the effect of DNA structure and chromosomal protein on Cas 9 binding with a distinct classification of Cas types. …”
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CRISPR/Cas9, a universal tool for genomic engineering by A. V. Smirnov, A. M. Yunusova, V. A. Lukyanchikova, N. R. Battulin

“…First, we describe the basic mechanism of the CRISPR/Cas9 DNA recognition and binding, focusing in particular on its off-target activity. …”
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Impact of Combined Antiretroviral Treatment (cART) on Latent Cytomegalovirus Infection by Aura Temereanca, Luminita Ene, Gratiela Tardei, Camelia Grancea, Cristian L. Achim, Simona Ruta

“…No differences were observed between patients with suppressed/non-suppressed HIV viremia in terms of CMV humoral and cellular immune response. CMV DNA was detected in only 17% of participants, with lower levels among those with cART-induced immune recovery. …”
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Hypoxia Associated Proteolytic Processing of OS-9 by the Metalloproteinase Meprin β by Barry Lee Martin, Sabena Michelle Conley, Regine Simone Harris, Corshe Devon Stanley, Jean-Marie Vianney Niyitegeka, Elimelda Moige Ongeri

“…Transfection of kidney cells (MDCK and HEK293) with meprin β cDNA prevented accumulation of OS-9 following exposure to the hypoxia mimic, CoCl2. …”
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基于Transformer的东北虎体侧条纹个体识别 by 马光凯, 张静, 戴文锐, 刘梦雨, 刘丹, 姜广顺

“…东北虎（<italic>Panthera tigris altaica</italic>）作为世界上最大的猫科（Felidae）动物，同时也是濒危物种，其个体识别是回答进化生物学中许多重大问题的关键步骤。尽管目前已提出虹膜和DNA分析等传统方法用于东北虎个体识别，但这些方法在远程获取和样本收集方面面临挑战，且在很大程度上依赖人工识别。…”
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Treatment of Radiation Bone Injury with Transplanted hUCB-MSCs via Wnt/β-Catenin by Yufeng Zhang, Huaxin Deng, Zhiqiang Yang, Zhe Chen, Sheng Zhang, Yufan Zhu, Min Yang, Houcheng Zhong, Fuling Zhou, Yuanlong Xie, Lin Cai

“…We found that radiation can damage the DNA of osteoblasts; induce apoptosis; reduce the differentiation, migration, and adhesion of osteoblasts, leading to lipogenesis of bone marrow mesenchymal stem cells (BMSCs) and reducing the source of osteoblasts; and increase the number of osteoclasts in bone tissue, while MSC treatment prevents these changes. …”
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Advances in bioinformatics and multi-omics integration: transforming viral infectious disease research in veterinary medicine by Alyaa Elrashedy, Walid Mousa, Mohamed Nayel, Akram Salama, Ahmed Zaghawa, Ahmed Elsify, Mohamed E. Hasan

“…With significant advancements in bioinformatics and NGS, researchers can now identify contigs, which are contiguous sequences of DNA that are assembled from overlapping fragments, assemble a complete genome, perform phylogenetic analysis to diagnose, investigate the risk of viral diseases in animals, handle and share large biological datasets across various species. …”
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Sinistroporomonorchis bolini n. sp. (Trematoda: Monorchiidae) from the Ocellated killifish, Floridichthys polyommus (Cyprinodontidae) in the Yucatán Peninsula, Mexico based on an i... by Leopoldo Andrade-Gómez, Juan F. Espínola-Novelo, Brenda Solórzano-García, Gerardo Pérez-Ponce de León

“…In addition, sequences of the 28S of large subunit of nuclear ribosomal RNA and cox1 of the mitochondrial DNA were obtained. Phylogenetic trees inferred from each dataset, placed all the specimens in a monophyletic clade, confirming that the isolates belonged to the same species. …”
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A comparative study on germination of wheat grains with different anthocyanin pigmentation of the pericarp in natural or induced aging by E. I. Gordeeva, O. Y. Shoeva, E. K. Khlestkina

“…These sister lines carry different combinations of recombinant DNA regions (on chromosomes 2A and 7D) containing dominant and recessive alleles at loci Pp3 and Pp-D1 (Pp: Purple pericarp), which determine the anthocyanin color of coleoptiles and of the pericarp. …”
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Genes of Inflammation and Placental Function GWAS Associated with Idiopathic Recurrent Miscarriage in the Kazakh Population by Alexandra Murtazaliyeva, Gulnara Svyatova, Galina Berezina, Gulfairuz Urazbayeva, Aigerim Sadyrbekova

“…Materials and Methods: Molecular genetic studies were performed by TaqMan using a single site-specific amplification and real-time genotyping method in 302 women with iRM and 300 with normal reproduction. DNA isolation from the biomaterial was carried out using kits containing binding magnetic particles. …”
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Different Pathological Roles of Toll-Like Receptor 9 on Mucosal B Cells and Dendritic Cells in Murine IgA Nephropathy by Tadahiro Kajiyama, Yusuke Suzuki, Masao Kihara, Hitoshi Suzuki, Satoshi Horikoshi, Yasuhiko Tomino

“…To address this, we nasally challenged cell-specific CpG DNA ((i): dendritic cell: (DC), (ii): B cell, (iii): both), known as ligand for TLR9, to IgAN prone mice and analyzed disease phenotype of each group. …”
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Arbuscular Mycorrhizal Fungi Associated with Myrciaria dubia in the Amazonia Region, Peru by Alexandra Jherina Pineda-Lázaro, Adela Vallejos-Tapullima, Angel David Hernández-Amasifuen, Santos Carballar-Hernández, Sixto Imán-Correa, Fernando Marcelo Carvajal-Vallejos, Orlando Ríos-Ramírez, Mike Anderson Corazon-Guivin

“…Likewise, the molecular analysis detected the presence of genetic material (rDNA) corresponding to AMF in the roots of both accessions. …”
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