Post-translational modifications as a key mechanism for herpes simplex virus type I evasion of host innate immunity by Yongxing Zhang, Junlei Xie, Ying Feng, Abdul Qadeer, Shanni Li, Xu Deng, Lipeng Zhu, Bo Kong, Zanxian Xia, Zanxian Xia

“…Herpes simplex virus type 1 (HSV-1) is a DNA virus that infects humans and establishes long-term latency within the host. …”
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Identifikasi Ekspresi Gen Myc pada Subkultur MCF-7 Breast Cancer Cell Line dengan Sel Punca by Yuastika Puspita Sari, Hirowati Ali, Aswiyanti Asri

“…Kemudian, dilakukan isolasi RNA, sintesis cDNA, dan ekspresi Myc diperiksa menggunakan PCR dan elektroforesis. …”
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Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis by Fatemeh Zare Shahneh, Samira Valiyari, Abbas Azadmehr, Reza Hajiaghaee, Saeid Yaripour, Ali Bandehagh, Behzad Baradaran

“…Cell death ELISA was employed to quantify the nucleosome production result from nuclear DNA fragmentation during apoptosis and determine whether the mechanism involves induction of apoptosis or necrosis. …”
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Hologenomic data generation and analysis in wild vertebrates by Carlotta Pietroni, Nanna Gaun, Aoife Leonard, Jonas Lauritsen, Garazi Martin‐Bideguren, Iñaki Odriozola, Ostaizka Aizpurua, Antton Alberdi, Raphael Eisenhofer

“…We also report that birds and bats both have substantially lower microbial DNA fractions and a higher degree of sample‐to‐sample variability compared to amphibians, reptiles and non‐flying mammals. …”
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LongReadSum: A fast and flexible quality control and signal summarization tool for long-read sequencing data by Jonathan Elliot Perdomo, Mian Umair Ahsan, Qian Liu, Li Fang, Kai Wang

“…We also demonstrate a few examples using LongReadSum to analyze cDNA sequencing, direct RNA sequencing, ONT reduced representation methylation sequencing (RRMS), and whole genome sequencing (WGS) data.…”
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Prevalence of Sero-Molecular Markers of Hepatitis C and B Viruses among Patients with β-Thalassemia Major in Northern West Bank, Palestine by Kamal Dumaidi, Amer Al-Jawabreh, Fekri Samarah, Maha Rabayaa

“…Serum sample from 139 multitransfused β-thalassemia patients were tested for HBV and HCV markers including HBsAg, anti-HBc, anti-HBs, HBV-DNA, and anti-HCV and HCV-RNA. Demographic data and selected clinical parameters were collected by means of a questionnaire and from the patients’ medical files. …”
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Impact of alemtuzumab-mediated lymphocyte depletion on SIV reservoir establishment and persistence. by Benjamin Varco-Merth, Morgan Chaunzwa, Derick M Duell, Alejandra Marenco, William Goodwin, Rachel Dannay, Michael Nekorchuk, Danica Shao, Kathleen Busman-Sahay, Christine M Fennessey, Lorna Silipino, Michael Hull, William J Bosche, Randy Fast, Kelli Oswald, Rebecca Shoemaker, Rachele Bochart, Rhonda MacAllister, Caralyn S Labriola, Jeremy V Smedley, Michael K Axthelm, Miles P Davenport, Paul T Edlefsen, Jacob D Estes, Brandon F Keele, Jeffrey D Lifson, Sharon R Lewin, Louis J Picker, Afam A Okoye

“…However, treatment was followed by proliferation and reconstitution of CD4+ T cells in blood, and despite ongoing ART, levels of cell-associated SIV DNA in blood and lymphoid tissues were not substantially different between alemtuzumab-treated and control RM after immune cell reconstitution, irrespective of the time of alemtuzumab treatment. …”
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Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus by Zhimin Ju, Jiajun Luo, Charles Brighton Ndandala, Chongqing Wen, Zhao Yanfei, Huafang Liang

“…Two subtypes of the Crustacean Hyperglycemic Hormone (CHH) gene and the cDNA of Panulirus homarus (PhCHHA and PhCHH-B) were been successfully cloned and characterized in this study. …”
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Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors by Catia Pilon, Andrea Rebellato, Riccardo Urbanet, Vincenza Guzzardo, Rocco Cappellesso, Hironobu Sasano, Ambrogio Fassina, Francesco Fallo

“…Methylation of CpG-rich 5′ regions was assessed by bisulfite sequencing PCR using bisulfite-treated DNA from archival microdissected paraffin-embedded adrenocortical tissues. …”
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Supervised machine learning and genotype by trait biplot as promising approaches for selection of phytochemically enriched Rhus coriaria genotypes by Hamid Hatami Maleki, Reza Darvishzadeh, Ahmad Alijanpour, Yousef Seyfari

“…Fruits of 75 sumac genotypes (15 genotype per population) were analyzed using HPLC-LC/MS-MS method. Likewise, genomic DNA of 75 genotypes were fingerprinted using 18 ISSR primers. …”
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Conducting and automating the water Morris maze test in SPF conditions by N. V. Khotskin, V. A. Kulikov, E. L. Zavyalov, D. V. Fursenko, A. V. Kulikov

“…With this installation, we were able to study the spatial ability to learn and spatial memory in mice of the C57BL/6 strain and in mice of the C57BL/6/ Kaiso strain developed on the C57BL/6 background, with the gene encoding the methyl-DNA binding Kaiso protein knocked-out. It has been demonstrated that mice of these strains are able to learn to find the platform in the water Morris maze and have the location of the platform in their memory for at least the next four days.…”
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Intricacies for Posttranslational Tumor-Targeted Cytokine Gene Therapy by Jeffry Cutrera, Denada Dibra, Arun Satelli, Xuexing Xia, Shulin Li

“…Electroporation-mediated transfection of cytokine plasmid DNA including a tumor-targeting peptide-encoding sequence is one method for obtaining a tumor-targeted cytokine produced by the tumor-bearing patient’s tissues. …”
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Deterministic genetic barcoding for multiplexed behavioral and single-cell transcriptomic studies by Jorge Blanco Mendana, Margaret Donovan, Lindsey Gengelbach O'Brien, Benjamin Auch, John Garbe, Daryl M Gohl

“…This method, called Targeted Genetically-Encoded Multiplexing (TaG-EM), involves inserting a DNA barcode just upstream of the polyadenylation site in a Gal4-inducible UAS-GFP construct so that the barcode sequence can be read out during single-cell sequencing, labeling a cell population of interest. …”
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Regulation of Tumor Immunity by Tumor/Dendritic Cell Fusions by Shigeo Koido, Sadamu Homma, Eiichi Hara, Yoshihisa Namiki, Akitaka Takahara, Hideo Komita, Eijiro Nagasaki, Masaki Ito, Toshifumi Ohkusa, Jianlin Gong, Hisao Tajiri

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Evaluation of common COL2A1 gene variants in Iranian patients suspected with Stickler syndrome type I by Fatemeh Abolhasani, Hossein Abdali, Mohammad Kazemi, Bijan Movahedian Attar, Fatemeh Derakhshandeh, Majid Hosseinzadeh

“…Materials and Methods: Twenty-six Iranian patients suspected of having Stickler syndrome type I referring to Al-Zahra Hospital of Isfahan were employed in this cross-sectional study. The DNA was extracted from the patient’s peripheral blood samples, and the selected exons of the COL2A1 gene were amplified by polymerase chain reaction. …”
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Protein interference for regulation of gene expression in plants by A. O. Vyacheslavova, I. A. Abdeeva, E. S. Piruzian, S. A. Bruskin

“…TFs usually consist of three protein domains required for DNA binding, dimerization, and transcriptional regulation. …”
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Molecular monitoring of short- and long-term transcriptional effects of hair growth stimulating agents. by Sabrina K Henne, Lara M Hochfeld, Werner Bartmann, Thomas Welss, Markus M Nöthen, Stefanie Heilmann-Heimbach

“…Genotyping was performed on DNA extracted from blood or saliva samples. Differential expression analyses identified 52 differentially expressed genes and 17 modulated pathways following treatment with the three hair growth stimulating agents. …”
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Emergence of Fusarium incarnatum and Fusarium avenaceum in wilt affected solanaceous crops of the Northern Himalayas by Tasmeen J. Parihar, Madeeha Naik, Shafqat Mehraj, Syed Inam ul Haq, Maqsooda Perveen, Ishfaq Ahmed Malla, Taniya Abid, Nadia Gul, Khalid Z. Masoodi

“…Through both morphological and molecular analyses, including DNA barcoding of the ITS, TEF, RPB1, and RPB2 genomic regions, Fusarium incarnatum and Fusarium avenaceum were identified as the primary causal agents of wilt in tomato and brinjal, and chili, respectively. …”
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Anticancer Potential of Fruit Extracts from Vatica diospyroides Symington Type SS and Their Effect on Program Cell Death of Cervical Cancer Cell Lines by Atchara Chothiphirat, Kesara Nittayaboon, Kanyanatt Kanokwiroon, Theera Srisawat, Raphatphorn Navakanitworakul

“…Treatment of ACE with half IC50 caused DNA fragmentation and also activated increasing of bax and cleaved caspase-8 protein in HeLa cells after 48 h exposure. …”
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Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia by Xiuchun Yang, Yijun Zong, Zhentian Zhang, Yan Zhao, Xueying Gao, Jie Zhang, Qian Hou, Renyi Li, Bing Xiao

“…Peripheral venous blood samples from patients with CAE were collected for RNA sequencing to identify differentially expressed genes (DEGs), followed by functional enrichment. Then, the DNA methylation profile of CAE was downloaded from GSE87016 (HumanMethylation450 BeadChip data, involving 11 cases and 12 normal controls) to identify differentially methylated genes (DMGs). …”
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