The value of increasing sequencing depth for noninvasive prenatal screening for whole chromosomal aneuploidy by Dongmei Wang, Fangfang Guo, Yaping Hou, Haishan Peng, Tingting Hu, Jiexia Yang

“…This study recruited a cohort of 59,800 singleton pregnancies from Guangdong Women and Children Hospital between January 2015 and December 2020, including 48,018 cases of NIPT and 11,782 cases of expanded NIPT. Cell-free DNA from plasma samples was sequenced at a sequencing depth of 0.15X for NIPT and 0.4X for expanded NIPT. …”
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Analysis of Visual Field Defects Obtained with Semiautomated Kinetic Perimetry in Patients with Leber Hereditary Optic Neuropathy by Katarzyna Nowomiejska, Agnieszka Kiszka, Edyta Koman-Wierdak, Katarzyna Tonska, Ryszard Maciejewski, Anselm G. Jünemann, Robert Rejdak

“…Twenty-two eyes of eleven consecutive LHON male patients with confirmed mitochondrial 11778G>A DNA mutation were prospectively examined with the V4e stimulus of SKP in both eyes. …”
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Solving an enigma in the tree of life, at the origins of teleost fishes by Parey, Elise, Berthelot, Camille, Roest Crollius, Hugues, Guiguen, Yann

“…Notably, in addition to traditional molecular phylogeny methods based on the evolution of gene sequences, we also considered the evolution of gene order along the DNA molecule. We discuss the challenges and opportunities that these new markers represent for the field of molecular phylogeny, and in particular the possibilities they offer for re-examining other controversial branches in the tree of life.…”
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恶性疟原虫肝期抗原1 基因3′端的克隆及序列分析 by 单志新, 余新炳, 李学荣, 马长玲, 徐　劲, 陈守义

“…【方法】应用PCR 技术从FCC1/HN 株基因组DNA 中扩增LS A-1 3′端序列, 用T-A 克隆 法将其克隆入pMD18-T 载体。阳性克隆的重组质粒经酶切及PCR 扩增鉴定后, 用双脱氧链末端终止法进行基因序列测定。 …”
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Sex disparities in the association between rare earth elements exposure and genetic mutation frequencies in lung cancer patients by Mengyuan Liu, Jiali Zhang, Xiaohong Duan, Qiming Zhou, Jing Chen, Siyao Liu, Junyan Su, Li Han, Fan Yang, Niansong Qian

“…A cohort of 53 LUAD patients underwent tumor DNA sequencing (1123 cancer-related genes) and plasma REE (lanthanum (La), cerium (Ce), praseodymium (Pr), neodymium (Nd), and yttrium (Y)) quantification. …”
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Polymorphism T3111C of the Clock gene in ethnic groups of women from Russia and Buryatia by O. V. Kaluzhnaya, N. V. Semenova, T. A. Bairova, I. M.  Madaeva, E. V. Belyaeva, L. I. Kolesnikova

“…To this end, we conducted genotyping of DNA samples by polymerase chain reaction. It was shown that the compared groups have statistically significant differences in genotypes frequency (р = 0.001). …”
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Prokaryotic communities associated with marine hydrothermal systems of the Gulf of California by Ruth Noemí Aguila-Ramírez, Bárbara González-Acosta, Karla María Gutiérrez-Almada, José Manuel Borges-Souza, Rocío Guadalupe Cervantes-Gámez, Eduardo Quiróz-Guzmán

“…This study investigates the microbial communities in three MHS sites in Baja California Sur, Mexico.MethodsSediment samples were collected in two seasons of the year: rainy and dry season. Bacterial DNA was extracted, the V3-V4 regions of the 16S rRNA gene were amplified.Results and discussionThe analysis of microbial community structure and composition revealed that species richness and diversity were higher at control sites (not influenced by hydrothermal conditions). …”
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Structuring the genetic collection of sunflower by V. A. Gavrilova, I. N. Anisimova, N. V. Alpatieva, E. A. Porokhovinova

“…A set of 237 sunflower lines from VIR’s genetic collection was phenotyped for their morphological characters (plant height; branching type; shapes and sizes of the leaf, petiole, flower head, disk and ray florets; downy mildew damage rate) and genotyped with molecular markers. DNA markers were used to identify accessions carrying the Rf gene and CMS. …”
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Complete chloroplast genomes of 13 species of the Impatiens genus for genomic features and phylogenetic relationships studies by Qinqin Yong, Meijun Li, Zhi Li, Chao Luo, Jinling Zhang, Xinxiang Bai

“…Five mutation hotspots were detected: rps16-trnQ-UUG, ndhF, ccsA-ndhD, ycf1, and trnN-GUU, among which ycf1 had the highest Pi value and the greatest potential as a DNA barcode marker. Our phylogenetic tree shows that all 13 species belong to Section Impatiens. …”
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Molecular mechanisms and therapeutic strategies in overcoming chemotherapy resistance in cancer by Yixiang Gu, Ruifeng Yang, Yang Zhang, Miaomiao Guo, Kyle Takehiro, Ming Zhan, Linhua Yang, Hui Wang

“…This review provides a comprehensive analysis of the key molecular mechanisms underlying chemotherapy resistance, including drug efflux, enhanced DNA damage repair (DDR), apoptosis evasion, epigenetic modifications, altered intracellular drug metabolism, and the role of cancer stem cells (CSCs). …”
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Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing by Youngil Koh, Daeyoon Kim, Woo-June Jung, Kwang-Sung Ahn, Sung-Soo Yoon

“…Materials and Methods. We extracted DNA from SNU_MM1393_BM and SNU_MM1393_SC and performed WES. …”
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C-Myc／Fas基因转染对人颊癌细胞移植瘤的影响 by 侯劲松, 黄洪章, 王建广, 潘朝斌, 唐海阔

“…分别瘤内注射脂质体、脂质体，pBK-fas、以及脂质体／pBK-Fas／pcDNA3-C-Myc共聚物，观测移植瘤增殖，绘制生长曲线。转染72h后每组处死6只动物，RT-PCR检测移植瘤细胞FasmRNA表达。…”
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Assessment of relationships between epigenetic age acceleration and multiple sclerosis: a bidirectional mendelian randomization study by Hongwei Liu, Hanqing Zhang, Zhaoxu Yin, Miaomiao Hou

“…Abstract Background The DNA methylation-based epigenetic clocks are increasingly recognized for their precision in predicting aging and its health implications. …”
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p38 MAPK 抑制剂通过抑制JNK 活性抑制培养的小脑颗粒神经元凋亡 by 黎明涛, 王文雅, 孙　娟, 唐孝礼, 苏兴文, 邱鹏新, 颜光美

“…【方法】把体外培养的小脑颗粒神经元从含去极化浓度钾离子(KCl 25 mmo l·L-1)的培养基中转移至低钾培养基(KCl 5 mmol·L-1)中诱导神经元凋亡。凝胶电泳分析DNA 片段, SAPK/ JNK 分析盒测定c-Jun N-末端蛋白激酶(JNK)活性。【结 果】低钾诱导小脑颗粒神经元的具有典型形态学和生化特征的凋亡。…”
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Pan-Cancer Analysis of the Prognostic and Immunotherapeutic Value of PDGFB by Bai Y, Wang X, Wang B

“…The study also demonstrated that PDGFB was linked to RNA and DNA stemness in 15 and 36 tumor types, respectively, and had a positive association with tumor lymphocyte infiltration. …”
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Chromosome‐scale genome assembly of Poa trivialis and population genomics reveal widespread gene flow in a cool‐season grass seed production system by Caio A. C. G. Brunharo, Christopher W. Benson, David R. Huff, Jesse R. Lasky

“…The genome assembly resulted in 1.35 Gb of DNA sequence distributed among seven chromosome‐scale scaffolds, revealing a high content of transposable elements, conserved synteny with Poa annua, and a close relationship with other C3 grasses. …”
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人类Foxm1b基因的原核表达、抗体制备及其对肝癌细胞中Foxm1b蛋白的识别 by 唐保东, 刘思纯, 徐雅, 曾志荣, 胡品津

“…【方法】从GenBank中下载人类Foxm1b基因全长cDNA序列并用Pegene软件分析其可能的抗原表位，设计引物．应用RT—PCR获得含抗原表位的Foxm1b基因片段，重组人原核表达载体pET-28a，在大肠杆菌BL-21（DE31中诱导表达，应用亲和层析法获得纯度较高的原核表达蛋白并免疫大鼠制备多克隆抗体，用ELISA检测抗体滴度并用免疫细胞化学的方法检测此抗体对肝癌细胞中天然Foxm1b蛋白的识别。…”
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Cystic Echinococcosis/Hydatid Cyst Coinfection with HIV: A Report from Shiraz, Iran by Yosef Sharifi, Seyed Mahmoud Sadjjadi, Hamed Nikoupour Dailami, Seyed Hamed Jafari, Mohammad Hossein Anbardar, Mohammad Bagher Khosravi

“…The IgG ELISA test with native AgB for E. granulosus infection was also positive. mtDNA amplification using PCR and sequencing showed the cyst as E. granulosus sensu stricto genotype. …”
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Mitotic block and epigenetic repression underlie neurodevelopmental defects and neurobehavioral deficits in congenital heart disease by George C. Gabriel, Hisato Yagi, Tuantuan Tan, Abha Bais, Benjamin J. Glennon, Margaret C. Stapleton, Lihua Huang, William T. Reynolds, Marla G. Shaffer, Madhavi Ganapathiraju, Dennis Simon, Ashok Panigrahy, Yijen L. Wu, Cecilia W. Lo

“…Transcriptome profiling, DNA methylation, and Sap130 ChIPseq analyses all demonstrate dysregulation of genes associated with autism and cognitive impairment. …”
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Analysis of the transcriptional activity of model piggyBac transgenes stably integrated into different loci of the genome of CHO cells in the absence of selection pressure by L. A. Yarinich, A. A. Ogienko, A. V. Pindyurin, E. S. Omelina

“…Each individual transgene contained a unique tag, a DNA barcode, and the resulting polyclonal cell population was cultured for almost a month without any selection. …”
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