The Chromatin Pattern of Cell Nuclei Is of Prognostic Value for Renal Cell Carcinomas by Christine François, Myriam Remmelink, Michel Petein, Roland van Velthoven, André Danguy, Eric Wespes, Isabelle Salmon, Robert Kiss, Christine Decaestecker

“…Thirty morphonuclear and 8 nuclear DNA content‐related variables were thus generated. …”
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Genistein Implications in Radiotherapy: Kill Two Birds with One Stone by Xiongxiong Liu, Tong Zheng, Yanyu Bao, Ping Li, Ting Zhao, Yan Liu, Hui Wang, Chao Sun

“…Conversely, genistein increases the radiosensitivity of tumor cells through specific mechanisms such as the inhibition of DNA repair, the arrest of the cell cycle in the G₂/M phase, the generation of reactive oxygen species (ROS), and the modulation of apoptosis. …”
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A case report of hermaphrodite goat: clinical and genetic approach by Çağlayan Özel, Gonca Şen, Mustafa Hitit, Nadir Koçak, Aydın Güzeloğlu, Ahmet Semacan, Ercan Kurar, Ercan

“…After chromosome preparation using peripheral blood samples and GTG staining, appropriate metaphases were analyzed using an image analyzer program. Genomic DNA was isolated from the blood sample. Sexdetermining region Y (SRY) and amelogenin gene regions were amplified using polymerase chain reaction (PCR) including positive and negative controls. …”
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血管紧张素Ⅱ诱导心肌细胞肥大后缝隙连接蛋白Cx43表达的变化 by 杨军, 伍卫, 梁蔚文, 王景峰, 潘秋辉, 方昶, 黄至斌

“…[结果]血管紧张素Ⅱ处理后的心肌细胞表现细胞肥大且细胞活力增强,S期、G2-M期细胞百分比增加,细胞内G2-M（二倍体）DNA含量降低,Cx43蛋白表达低于对照组,Cx43mRNA表达水平也较对照组明显下调,Cx43蛋白表达下调与细胞周期分布的改变相关。…”
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Biomonitoring with Micronuclei Test in Buccal Cells of Female Farmers and Children Exposed to Pesticides of Maneadero Agricultural Valley, Baja California, Mexico by Idalia Jazmin Castañeda-Yslas, María Evarista Arellano-García, Marco Antonio García-Zarate, Balam Ruíz-Ruíz, María Guadalupe Zavala-Cerna, Olivia Torres-Bugarín

“…Previous papers have presented contradictory information with respect to indirect exposure to pesticides and DNA damage. We aimed to evaluate the genotoxic effect in buccal mucosa from female farmers and children, working in the agricultural valley of Maneadero, Baja California. …”
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Pancreatic Beta Cell Death: Novel Potential Mechanisms in Diabetes Therapy by Joselyn Rojas, Valmore Bermudez, Jim Palmar, María Sofía Martínez, Luis Carlos Olivar, Manuel Nava, Daniel Tomey, Milagros Rojas, Juan Salazar, Carlos Garicano, Manuel Velasco

“…They also include the action of proinflammatory cytokines, the production of reactive oxygen species, DNA fragmentation (typical of necroptosis in type 1 diabetic patients), excessive production of islet amyloid polypeptide with the consequent endoplasmic reticulum stress, disruption in autophagy mechanisms, and protein complex formation, such as the inflammasome, capable of increasing oxidative stress produced by mitochondrial damage. …”
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Distribution of the arctic variant of the CPT1A gene in indigenous populations of Siberia by B. A. Malyarchuk, M. V. Derenko, G. A. Denisova, A. N. Litvinov

“…Five polymorphic loci relevant to the haplotypic structure of CPT1A gene (rs2278908, rs2278907, rs2924699, rs7112615 and rs2229738) were revealed by high-throughput DNA sequencing in addition to locus rs80356779 studied here. …”
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THE EFFECT OF QUERCETIN ON MORPHOLOGICAL AND BIOCHEMICAL CHANGES IN RAT LIVER UNDER 270TH DAY CENTRAL DEPRIVATION OF LUTEINIZING HORMONE SYNTHESIS by M.V. Rud, V.I. Shepitko, Ye.V. Stetsuk, O.Ye. Akimov

“…Peroxynitrite and superoxide anion radical are powerful oxidants that can damage biological polymers (DNA, proteins and biological membranes) and lead to the development of oxidative-nitrosative stress.…”
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Genomic, Evolutionary, and Pathogenic Characterization of a New <i>Polerovirus</i> in Traditional Chinese Medicine <i>Viola philippica</i> by Yuanling Chen, Gaoxiang Chen, Jiaping Yu, Yali Zhou, Shifang Fei, Haorong Chen, Jianxiang Wu, Shuai Fu

“…Two potential recombination events were identified within ORF2, ORF3a, and ORF3, providing insights into the genetic diversity and evolution history of this novel polerovirus. An infectious cDNA clone of VPPV was successfully constructed and shown to infect <i>Nicotiana benthamiana</i>. …”
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Тransplastomic tobacco plants producing the hydrophilic domain of the sheep pox virus coat protein L1R by D. K. Beisenov, G. E. Stanbekova, B. K. Iskakov

“…Southern blot analysis showed heteroplasmy of the plastids in the obtained plants due to recombination events between native and introduced regulatory plastid DNA elements. The recombinant protein from plant tissue was purified using metal affinity chromatography. …”
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Epigenetic Regulation of Stromal and Immune Cells and Therapeutic Targets in the Tumor Microenvironment by Kang Liu, Yue Li, Minmin Shen, Wei Xu, Shanshan Wu, Xinxin Yang, Bo Zhang, Nengming Lin

“…This review synthesizes recent research that elucidates the impact of specific epigenetic regulations—ranging from DNA methylation to histone modifications and chromatin remodeling—on stromal and immune cells within the TME. …”
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DMD基因内含子的重复序列与内含子不稳定性和基因缺失的关联 by 盛文利

“…【结论】重复序列是DMD基因多数内含子的关键结构,与内含子的不稳定性相关。在原有DNA序列的基础上,单链发夹结构的形成增加了DMD基因的不稳定性,形成了基因断裂的基础,单链发夹结构的形成导致两个断裂点的靠近,最终导致了基因缺失。…”
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Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms by Hongyu Xu, Zetian Jia, Junhui Liu, Runming Liu, Wei Wei, Xiang Li

“…We describe steps for sample collection, fecal genomic DNA extraction, rRNA PCR amplification, sequencing library construction, and rRNA sequencing. …”
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Prevalence and Subtype Analysis of Blastocystis hominis Isolated from Patients in the Northeast of Iran by Mitra Salehi, Jalal Mardaneh, Hamid Reza Niazkar, Mohammadhaasan Minooeianhaghighi, Elahe Arshad, Fateme Soleimani, Alireza Mohammadzadeh

“…Samples were examined by a wet mount, and genomic DNA was extracted from positive samples. Also, PCR was done on the positive samples, and sequencing and phylogenetic analysis were subsequently performed. …”
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In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products by Guesh Mulaw, Tesfaye Sisay Tessema, Diriba Muleta, Anteneh Tesfaye

“…The four efficient probiotic LAB isolates that belonged to Lactobacillus species were identified to the strain level using 16S rDNA gene sequence comparisons and, namely, were Lactobacillus plantarum strain CIP 103151, Lactobacillus paracasei subsp. tolerans strain NBRC 15906, Lactobacillus paracasei strain NBRC 15889, and Lactobacillus plantarum strain JCM 1149. …”
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Heavy metal contamination and eutrophication effects on bacterial communities in the Han River basin by Fei Xiong, Cejia Yao, Dongdong Zhai, Hongyan Liu, Wang Dong, Yuanyuan Chen

“…This study analyzed microbial communities in sediment samples from 15 sites using 16S rDNA sequencing. Our findings revealed a significant decrease in α-diversity with increasing copper (Cu) concentration, with the downstream region (group L) exhibiting higher diversity compared to the upstream region (group U). …”
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Pyrogallol acts as a novel anticancer factor to enhance the sensitivity to cisplatin in ovarian cancer cells through inducing miR-15a upregulation by Azin Shirani, Razieh Mirabbasi, Leila Shabani, Somayeh Reiisi

“…Cisplatin is known as an effective chemotherapeutic agent against cancer by inducing DNA damage and apoptosis. However, the effect of pyrogallol alone and in combination with cisplatin on proliferation and apoptosis of ovarian cancer has not yet been reported. …”
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Creation of transgenic mice susceptible to coronaviruses: a platform for studying viral pathogenesis and testing vaccines by N. R. Battulin, O. L. Serov

“…Therefore, to create mice susceptible to SARS-CoV and SARS-CoV2 coronaviruses, the human ACE2 gene is transferred into their genome. The exogenous DNA of the constructs is inserted into the recipient genome randomly and with a varying number of copies. …”
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Whole genome sequencing completes the molecular genetic testing workflow of patients with Lynch syndrome by Klaudia Horti-Oravecz, Anikó Bozsik, Tímea Pócza, Ildikó Vereczkey, Tamás Strausz, Erika Tóth, Tatiana Sedlackova, Diana Rusnakova, Tomas Szemes, István Likó, Edit Oláh, Henriett Butz, Attila Patócs, János Papp, Vince Kornél Grolmusz

“…Abstract Multigene panel tests (MGPTs) revolutionized the diagnosis of Lynch syndrome (LS), however noncoding pathogenic variants (PVs) can only be detected by complementary methods including whole genome sequencing (WGS). Here we present a DNA-, RNA- and tumor tissue-based WGS prioritization workflow for patients with a suspicion of LS where MGPT detected no LS-related PV. …”
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Different electrode system based electrochemical aptasensor for the detection of dengue virus antigen in human serum: A comparative study by Mohd Rahil Hasan, Pradakshina Sharma, Saumitra Singh, Sushil Kumar, Shivani, Kirti Rani, Jagriti Narang

“…Both the electrode types were coated with chemically synthesized ZnO-Nanomaterial, which aids in electron transport, and to make it more selective highly specific DNA-aptamer was used against the DENV antigen. SEM and Uv–Vis spectra approaches were used to characterize the synthesized nanomaterial. …”
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