Impact of Hepatitis B Exposure on Sustained Virological Response Rates of Highly Viremic Chronic Hepatitis C Patients by Ioannis S. Elefsiniotis, Christos Pavlidis, Elena Vezali, Theodoros Mariolis-Sapsakos, Sotirios Koutsounas, George Saroglou

“…Anti-HBc positivity was more prevalent in cirrhotic, compared to noncirrhotic patients (76.9% versus 19.5%, P<.05). Serum HBV-DNA was detected in the minority of anti-HBc-positive patients (1.97%). …”
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Analysis of MicroRNA Processing Machinery Gene DROSHA, DICER1, and XPO5 Variants Association with Atherosclerosis: A Case–control Study by Manar F. Atoum, Dalya Alowaisy, Ammar Ali Deeb

“…Methods: The study included 100 individuals with clinically diagnosed atherosclerosis (mean age: 53 ± 7 years) and 100 control subjects (mean age: 52 ± 7 years). Genomic DNA was extracted and genotyped using real-time TaqMan allelic discrimination assays for DROSHA rs10719, DICER rs1057035, and XPO5 rs11077 SNPs. …”
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Diverse vaginal microbiota in healthy Japanese women: a combined relative and quantitative analyses by Masahiro Ito, Misaki Kataoka, Yoichi Sato, Hideki Nachi, Koji Nomoto, Koji Nomoto, Nobuhiko Okada

“…Vaginal fluid pH was measured using a portable pH meter. DNA was extracted from the vaginal fluid, and the 16S ribosomal RNA gene sequences in the V3-V4 regions were analyzed to identify bacterial species. …”
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Parasites of the genus Nosema, Crithidia and Lotmaria in the honeybee and bumblebee populations: a case study in India by V. Y. Vavilova, I. Konopatskaia, S. L. Luzyanin, M. Woyciechowski, A. G. Blinov

“…Recent  studies of nuclear DNA markers of these parasites allowed to describe new species and genetic variants. …”
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亚洲牛带绦虫钙调神经磷酸酶B基因的原核表达 和免疫学分析

“…【目的】 对亚洲牛带绦虫钙调神经磷酸酶B基因(CaN)进行克隆65380;表达和免疫学初步研究65377;【方法】 将亚洲牛带绦虫成虫钙调神经磷酸酶B基因克隆到原核表达质粒pET*9鄄30a(+)中,在大肠杆菌BL*9鄄21/DE3中用诱导表达,表达产物通过十二烷基磺酸钠*9鄄聚丙烯酰胺凝胶电泳(SDS*9鄄PAGE)进行鉴定,用镍离子金属螯合剂亲和层析柱进行纯化,纯化的重组蛋白用蛋白印迹进行免疫学分析65377;【结果】 PCR65380;双酶切及DNA测序结果均表明重组质粒pET*9鄄30a(+)*9鄄Ta CaN构建成功65377;SDS*9鄄PAGE结果表明目的基因能在大肠杆菌BL*9鄄21/DE3中表达,经亲和层析得到了纯化重组蛋白65377;重组蛋白能被感染了亚洲牛带绦虫的猪和患者的血清识别65377;【结论】 亚洲牛带绦虫成虫钙调神经磷酸酶B基因可在原核表达系统中获得具有免疫学活性的表达65377;为进一步研究该蛋白的功能奠定了基础65377;…”
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Scientific provision of an effective development of soybean breeding and seed production in the Russian Far East by V. T. Sinegovskaya

“…Together with Japanese scientists from the University of Niigata, the astragalus mosaic virus was detected on Canadian and Chinese varieties in Primorsky Krai and the Amur Region using DNA analysis. The carrier of this disease is soybean aphid (Aphis glycines).…”
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Current Review of Genetically Modified Lactic Acid Bacteria for the Prevention and Treatment of Colitis Using Murine Models by Alejandra de Moreno de LeBlanc, Silvina del Carmen, Jean-Marc Chatel, Anderson Miyoshi, Vasco Azevedo, Philippe Langella, Luis G. Bermúdez-Humarán, Jean Guy LeBlanc

“…Different delivery systems, including protein delivery and DNA delivery, will also be discussed. Studies show the efficacy of GM-LAB (using different expression systems) for the prevention and treatment of IBD, highlighting the importance of the bacterial strain selection (with anti-inflammatory innate properties) as a promising alternative. …”
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Risk Factors in Normal-Tension Glaucoma and High-Tension Glaucoma in relation to Polymorphisms of Endothelin-1 Gene and Endothelin-1 Receptor Type A Gene by Dominika Wróbel-Dudzińska, Ewa Kosior-Jarecka, Urszula Łukasik, Janusz Kocki, Agnieszka Witczak, Jerzy Mosiewicz, Tomasz Żarnowski

“…Methods. 285 Polish patients took part in the research (160 normal-tension glaucoma and 125 high-tension glaucoma). DNA was isolated by standard methods and genotype distributions of four polymorphisms in genes encoding endothelin-1 (K198N) and endothelin-1 receptor type A polymorphisms (C1222T, C70G, and G231A) were determined. …”
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Distribution and frequency of genetic mutations in three insecticide targets in field populations of Culex tritaeniorhynchus in Mianyang City, Sichuan Province, China by Hongwei Xie, Meilin Tang, Hongying Sun, Zhengzheng Huang, Mengmeng Dong, Xianying Wen

“…In the study, a total of 314 adults of Cx. tritaeniorhynchus, collected from 6 sites across Mianyang City, were inspected for resistance-conferring mutations in three genes encoding voltage-gated sodium channel (VGSC), Gamma-aminobutyric acid (GABA) receptor Rdl subunit (Rdl), and acetylcholinesterase (AChE) by DNA Sanger sequencing. The results showed that the classical L1014F mutation in VGSC was distributed in all the 6 populations at varying frequencies from 16.98% to 27.78%, and the frequency of F455W mutation in AChE was extremely high (97.06%-100%). …”
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Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes by Jue Lin, Joshua Cheon, Rashida Brown, Michael Coccia, Eli Puterman, Kirstin Aschbacher, Elizabeth Sinclair, Elissa Epel, Elizabeth H. Blackburn

“…Telomeres, the protective DNA-protein complexes at the ends of linear chromosomes, are important for genome stability. …”
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Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats by Lukáš Alán, Tomáš Olejár, Monika Cahová, Jaroslav Zelenka, Zuzana Berková, Magdalena Smětáková, František Saudek, Radoslav Matěj, Petr Ježek

“…Somatostatin analog octreotide completely blocked insulin secretion, impaired proliferation at low autocrine insulin, and decreased PPY secretion and mitochondrial DNA in INS-1E cells. In conclusion, in GK rats PI, significant local delta cell hyperplasia and suspected paracrine effect of somatostatin diminish beta cell viability and contribute to the deterioration of beta cell mass. …”
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A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae by Pavan Kumar, Giandomenico Corrado, Girigowda Manjunatha, Suma Ramegowda, Pradeep Kumar, Boris Basile, Ganadalu Puttaswamy Mutthuraju, Baggana Girish Jasmitha

“…Of the 151 bacterial isolates obtained from the pomegranate rhizosphere, three (UHSPS15A, UHSPS33, and UHSPS54) demonstrated the strongest inhibitory effects against Xap in vitro, and their identification as Pseudomonas was confirmed through DNA analysis. Greenhouse trials with Xap-inoculated plants revealed that preventive application of each of the three isolates was more effective than curative, with UHSPS15A providing the highest protection. …”
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Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal by Anicet Georges Bruno Manga, Godar Sene, André Amakobo Diatta, Tahir Abdoulaye Diop, Gérard Barroso, Diederik van Tuinen

“…Soil and root samples were collected from under <i>V. seyal</i> and in the vicinity of the trees. After DNA extraction, nested PCR, and sequencing of the large subunit region of the rRNA gene, different phylotypes from rhizospheric soils, roots, and spores were compared by phylogeny in order to investigate the role of salinity in arbuscular fungal diversity. …”
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Vaginal microbiome distinction in women with HPV+, cervical intraepithelial neoplasia, and cervical cancer, a retrospective study by Yuanyue Li, Yuanyue Li, Xiaomei Wu, Xiaomei Wu

“…This study aimed to investigate the correlation between the vaginal microbiota, HPV infection, cervical intraepithelial neoplasias (CINs), and cervical cancers in 69 women.MethodsDNA was extracted from vaginal samples, followed by HPV genotyping through PCR and sequenced of the16S rRNA gene.ResultsOur results revealed that Lactobacillus was the predominant bacterium across all groups, with prevalence rates of 60.2% in women with HPV+, 63.9% in CINI, 97.7% in CINII, 52.0% in CINIII, 36.9% in cervical cancer, and 70.9% in NILM (normal cytology). …”
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Genetic Diversity and Population Differentiation of the Causal Agent of Citrus Black Spot in Brazil by Ester Wickert, Antonio de Goes, Andressa de Souza, Eliana Gertrudes de Macedo Lemos

“…The objective of this work was study the genetic diversity and population differentiation of G. citricarpa associated with four sweet orange varieties in two geographic locations using DNA sequence of ITS1-5.8S-ITS2 region from fungi isolates. …”
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mRNA Expression of EgCHI1, EgCHI2, and EgCHI3 in Oil Palm Leaves (Elaeis guineesis Jacq.) after Treatment with Ganoderma boninense Pat. and Trichoderma harzianum Rifai by Laila Naher, Soon Guan Tan, Chai Ling Ho, Umi Kalsom Yusuf, Siti Hazar Ahmad, Faridah Abdullah

“…The data illustrated that chitinase cDNA expression varied depending on tissue and the type of treatment.…”
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DETECTION OF PORCINE CIRCOVIRUS TYPE 3 IN RUSSIAN PIG HOLDINGS by M. V. Biryuchenkova, A. M. Timina, A. V. Shcherbakov

“…Samples that were positive for PCV-3 DNA when tested with molecular methods (PCR, real-time PCR) were tested for other pathogens. …”
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Phylogenetic analysis of dermatophytes isolated from small domestic animals by A. M. Smagulova, E. V. Kukhar, T. I. Glotova, A. G. Glotov

“…The fungal isolate species were confirmed by sequencing using two rDNA internal transcribed spacer (ITS) primer pairs, and this allowed for their deposition to the GenBank database. …”
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Identification of KIFC1 as an independent prognostic marker in renal clear cell carcinoma correlates with tumor proliferation and immune infiltration by Bin Du, Jia Wang, Jinping Zheng, Jing Huo, Pu Wang

“…The expression of KIFC1 showed a significant positive correlation (Spearman coefficient > 0.7) with tumor proliferation-related pathways (tumor proliferation, G2/M checkpoint, and DNA replication) and tumor inflammation. Further, intratumoral immune cell analysis revealed that high expression of KIFC1 predicted more infiltration of CD8 + T and CD4 + T cells (p < 0.001). …”
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Multiple Roles of the Y Chromosome in the Biology of Drosophila melanogaster by Roberto Piergentili

“…This is due to the intrinsic nature of this genetic element, namely, (1) its molecular composition (mainly transposable elements and satellite DNA), (2) its genetic inertia (lack of recombination due to its heterochromatic nature), (3) the absence of homology with the X (with the only exception of the nucleolar organizer), (4) the lack of visible phenotypes when it is missing (indeed, except for their sterility, X0 flies are normal males), and (5) its low density as for protein-coding sequences (to date, only 13 genes out of approximately 14,000 have been mapped on this chromosome in D. melanogaster, i.e., ~0.1% of the total). …”
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