Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells by Fumiko Taniuchi, Koji Higai, Tomomi Tanaka, Yutaro Azuma, Kojiro Matsumoto

“…In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses the FUT1 gene, and shows α1,2-fucosyltransferase (FUT) activity. 5′-rapid amplification of cDNA ends revealed a FUT1 transcriptional start site −10 nucleotides upstream of the site registered at NM_000148 in the DataBase of Human Transcription Start Sites (DBTSS). …”
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Research Progress in Small-Molecule Detection Using Aptamer-Based SERS Techniques by Li Zheng, Qingdan Ye, Mengmeng Wang, Fan Sun, Qiang Chen, Xiaoping Yu, Yufeng Wang, Pei Liang

“…Nucleic acid aptamers are single-stranded oligonucleotides that are selected through exponential enrichment (SELEX) technology from synthetic DNA/RNA libraries. These aptamers can specifically recognize and bind to target molecules, serving as specific recognition elements. …”
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The beneficial effects of curcumin on aging and age-related diseases: from oxidative stress to antioxidant mechanisms, brain health and apoptosis by Ying He, Ying He, Yongqing Liu, Min Zhang

“…During the aging process, the accumulation of oxidative stress, DNA damage, telomere dysfunction, and other related changes lead to cellular dysfunction and the development of diseases such as neurodegenerative and cardiovascular conditions. …”
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IRAP-analysis for evaluating the genetic stability of endemic and endangered species of the Western Caucasus flora in the collection in vitro by I. I. Suprun, V. I. Malyarovskaya, I. V. Stepanov, L. S. Samarina

“…In Galanthus woronowii as well 8 of 16 IRAP primers resulted in the amplification with the number of DNA fragments ranging from 2 to 12. In Campanula sclerophylla 9 of 16 IRAP primers amplified 1 to 11 fragments, depending on the marker. …”
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Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog by Victor Paromov, Sudha Kumari, Marianne Brannon, Naga S. Kanaparthy, Hongsong Yang, Milton G. Smith, William L. Stone

“…Sulfur mustard or mustard gas (HD) and its monofunctional analog, 2-chloroethyl ethyl sulfide (CEES), or “half-mustard gas,” are alkylating agents that induce DNA damage, oxidative stress, and inflammation. …”
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亚甲基四氢叶酸还原酶多态性对结直肠癌患者化疗预后的影响

“…【方法】 收集经病理学确诊的Ⅳ期结直肠腺癌患者共８１例，接受标准的ＦＯＬＦＯＸ６方案或ＸＥＬＯＸ方案化疗，化疗前抽取患者外周血，采用ＤＮＡ测序的方法检测ＭＴＨＦＲ基因Ｃ６７７Ｔ和Ａ１２９８Ｃ多态性，比较不同基因型化疗后的无进展生存时间，并分析各种因素和预后的相关性。…”
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Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize (Zea mays L.) growth by Ebrahim Eshaghi, Sara Mousae, Ali Hendiyani, Alireza Habibi Khave, Rahim Nosrati

“…Biochemical characteristics, 16S rDNA amplification, and sequencing demonstrated a high similarity of PGPR isolates to the strains of Enterobacter, Pantoea, Kluyvera, Lelliottia, Klebsiella, Pectobacterium, and Stenotrophomonas. …”
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2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador. by Luis E Hernandez-Castro, Marta Paterno, Anita G Villacís, Björn Andersson, Jaime A Costales, Michele De Noia, Sofía Ocaña-Mayorga, Cesar A Yumiseva, Mario J Grijalva, Martin S Llewellyn

“…Here we assess the effectiveness of three different 2b restriction site-associated DNA (2b-RAD) genotyping strategies in R. ecuadoriensis to provide sufficient genomic resolution to tease apart microevolutionary processes and undertake some pilot population genomic analyses.…”
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HCV核心基因转染对QBC939细胞凋亡及细胞周期的影响 by 李志花

“…【方法】将包含HCV-C基因的真核表达质粒pcDNAHCV-C和空载体,利用脂质体介导将其转染QBC939,经G418筛选获得稳定转染HCV-C的QBC939细胞(QBC939HCV-C+),RT-PCR和免疫荧光法检测HCV-C蛋白表达,MTT法检测QBC939HCV-C+细胞、空白质粒转染QBC939(QBC939pcDNA)细胞和未转染QBC939细胞生长增生率;流式细胞术(FACS)检测3组细胞凋亡率和细胞周期,以及QBC939HCV-C+细胞经Fas抗体诱导凋亡后的细胞周期变化。…”
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Temporal and spatial characterization of keratinocytes supporting orf virus replication by Byung-Joon Seung, Sushil Khatiwada, Daniel L. Rock, Gustavo Delhon

“…Using in situ hybridization, immunohistochemistry, confocal microscopy, qPCR, and a full-thickness wound/infection model in sheep, the natural host, we show that during an initial 2-day eclipse phase viral transcription and viral DNA replication are not detected. Between days 2 and 3 pi, viral transcription is first detected in keratinocytes of the stratum granulosum and upper stratum spinosum in the proliferative zone at the wound margin. …”
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Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F... by Katja Tietze, Florian Brandt, Kerstin Wetzig, Lisa Hübinger, Marc Pretze, Ralph Alexander Bundschuh, Jörg Kotzerke

“…<b>Background:</b> Energy delivered at different wavelengths causes different types of damage to DNA. <b>Methods:</b> PC-3, FaDu, 4T1 and B16-F10 cells were irradiated with different wavelengths of ultraviolet light (UVA/UVC) and ionizing radiation (X-ray). …”
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Assessment of heat-killed E. coli expressing Chikungunya virus E2 protein as a candidate vaccine for dual protection against Chikungunya virus and E. coli by Surajit Patra, Surajit Patra, Virendra Gajbhiye, Virendra Gajbhiye, Yogesh A. Karpe, Yogesh A. Karpe

“…Escherichia coli (E.coli), a vital part of human gut microbiota, is utilized in recombinant DNA technology for cloning. However, its high adaptability can lead to severe infections in humans. …”
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重组野生型 p53 基因转染人骨肉瘤细胞株体外实验

“…目的: 将外源性野生型 p53 基因导入人骨肉瘤细胞株, 研究野生型 p53 蛋白在诱导瘤细胞凋亡中的作用。方法: 用 DNA 转染技术将 7 重组野生型 p53 基因 PM 47质粒导入人骨肉瘤细胞株 HOS-8603中, 用 PCR 技术监测外源性 p53 基因 在瘤细胞中存在的稳定性; 用免疫组化方法检测在地塞米松的诱导下瘤细胞中 p53 的表达情况; 用相差显微镜去观察凋亡瘤 细胞的形态特点; 用细胞凋亡原位检测技术标记并统计凋亡的瘤细胞数。…”
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Involvement of transposable elements in Alzheimer’s disease pathogenesis by R. N. Mustafin, E. K. Khusnutdinova

“…Our analysis of scientific literature in accordance with MDTE DB revealed 28 miRNAs derived from transposons (17 from LINE, 5 from SINE, 4 from HERV, 2 from DNA transposons), the expression of which specifically changes in this disease (decreases in 17 and increases in 11 microRNA). …”
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The known genetic variants of BRCA1, BRCA2 and NOD2 in pancreatitis and pancreatic cancer risk assessment by Jarosław Matykiewicz, Wioletta Adamus-Białek, Monika Wawszczak-Kasza, Bartosz Molasy, Magdalena Kołomańska, Rusłan Oblap, Łukasz Madej, Dorota Kozieł, Stanisław Głuszek

“…In our study, we analyzed these variants in a group of patients with pancreatitis and pancreatic cancer to clarify their role in pancreatic disease development. The DNA was isolated from whole blood samples of 553 patients with pancreatitis, 83 patients with pancreatic cancer, 44 cases of other pancreatic diseases, and 116 healthy volunteers. …”
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Eurotium Cristatum Postfermentation of Fireweed and Apple Tree Leaf Herbal Teas by Tatiana A. Efimenko, Elena F. Shanenko, Tatiana G. Mukhamedzhanova, Olga V. Efremenkova, Yuriy A. Nikolayev, Elena N. Bilanenko, Marina V. Gernet, Artem G. Grishin, Ivan N. Serykh, Sergey V. Shevelev, Byazilya F. Vasilyeva, Svetlana N. Filippova, Galina I. El-Registan

“…The species identification was carried out based on morphocultural characteristics and DNA sequencing. This study is aimed at determining the feasibility of making postfermented herbal teas using E. cristatum and to evaluate their quality. …”
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ULK1 methylation promotes TGF‐β1‐induced endometrial fibrosis via the FOXP1/DNMT1 axis by Yuhua Zeng, Qing Feng

“…This study will investigate the function and mechanism of forkhead box P1 (FOXP1)/DNA methyltransferase 1 (DNMT1)/unc‐51‐like autophagy activating kinase 1 (ULK1) in IUA. …”
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Clinical Characteristics and Prognostic Factors Affecting Clinical Outcomes in Cytomegalovirus Retinitis Following Allogeneic Hematopoietic Stem Cell Transplantation by Qiaozhu Zeng, Yuou Yao, Jing Hou, Heng Miao

“…Retinal detachment was significantly predicted by higher baseline aqueous CMV DNA load (OR 5.087, <i>p</i> = 0.026). Macula involvement (OR 5.322, <i>p</i> = 0.032) and more intravitreal injections (IVs) (OR 1.263, <i>p</i> = 0.008) were independent risk factors for recurrence. …”
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α-Bisabolol alleviates diesel exhaust particle-induced lung injury and mitochondrial dysfunction by regulating inflammatory, oxidative stress, and apoptotic biomarkers through the... by Sumaya Beegam, Nur Elena Zaaba, Ozaz Elzaki, Abderrahim Nemmar

“…Intratracheal instillation of DEPs induced DNA damage and increase in the apoptotic marker cleaved caspase-3. …”
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Diagnostics of glaucoma before clinical manifestations by N.E. Fomin, A.V. Kuroyedov

“…Among these methods is DNA testing, e.g., the identification of genetic mitochondrial dysfunction (mitochondria are the major producer of cellular energy). …”
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