‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes by Xingli Zhang, Chong Teng, Kaidi Lyu, Shanhua Lyu, Yinglun Fan

“…To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. …”
Get full text

Blood-based biomarkers in patients with non-small cell lung cancer treated with immune checkpoint blockade by Yo-Ting Tsai, Jeffrey Schlom, Renee N. Donahue

“…Here we review studies in which multiple blood-based biomarkers encompassing circulating soluble analytes, immune cell subsets, circulating tumor DNA, blood-based tumor mutational burden, and circulating tumor cells have shown promising associations with the clinical response of NSCLC patients to ICI therapy. …”
Get full text

Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase by A. A. Egorova, S. V. Gerasimova, A. V. Kochetov

“…The analysis of the OAT promoter structure reveals cis-acting regulatory DNA elements associated with auxin regulation and abiotic stresses. …”
Get full text

Pathogenic and therapeutic role of bile acids at reflux-gastritis by T. L. Lapina, I. M. Kartavenko, V. T. Ivashkin

“…Exposition of bile acids results in formation of active forms of oxygen and nitrogen, damage of DNA, mutagenicity, induction of apoptosis in short-term prospect and resistance to apoptosis in long-term prospect. …”
Get full text

Low-density SNP marker sets for genetic variation analysis and variety identification in cultivated citrus by Phuong Linh Nguyen, Jin-Kee Jung, Jee-Soo Park, Sung-Chur Sim

“…The resulting SNP sets are a resource to enhance the phenotype-based DUS testing by developing a DNA barcode system and thus facilitate new variety breeding and protection in citrus.…”
Get full text

Identification of Houge type of X-linked syndromic mental retardation caused by CNKSR2 truncated variants by Si-Hua Chang, Jie-Yuan Jin, Yi-Qiao Hu, Run-Yan Wang, Rong Xiang, Xia Wang

“…To verify the impact of these variants, we used real-time PCR and minigenes consisting of exon 14, intron 14, and exon 15 from both the wild-type and the c.1658-3_1676del DNA sequences. Results In this study, we reported two Chinese boys with MRXSHG and described some rare MRXSHG phenotypes, such as delayed bone age, slightly widened right fissure, and an underdeveloped right temporal lobe, characterized by reduced growth and volume compared to typical development. …”
Get full text

Multilocus phylogenies reveal three new truffle-like taxa and the traces of interspecific hybridization in Octaviania (Boletaceae, Boletales) by Takamichi Orihara, Rosanne Healy, Adriana Corrales, Matthew E. Smith

“…Here we describe two new species in subgenus Octaviania, O. tenuipes and O. tomentosa, from temperate to subtropical evergreen Fagaceae forests in Japan based on morphological observation and robust multilocus phylogenetic analyses (nrDNA ITS and partial large subunit [LSU], translation elongation factor 1-α gene [TEF1] and the largest subunit of RNA polymerase II gene [RPB1]). …”
Get full text

Characterization of the Fungal Microbiome (Mycobiome) in Fecal Samples from Dogs by M. Lauren Foster, Scot E. Dowd, Christine Stephenson, Jörg M. Steiner, Jan S. Suchodolski

Get full text

Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences by Yu-Hoon Kim, Sang Eon Shin, Chan Seon Ham, Seong Yoon Kim, Kwang Soo Ko, Tae-Ho Jo, Gi Hoon Son, Seong Hwan Park, Juck-Joon Hwang

“…We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea. After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed. …”
Get full text

Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granules by Noriko Ito, Yuai Li, Tsuneo Suzuki, Daniel J. Stechschulte, Kottarappat N. Dileepan

“…The exposure of the macrophage cell line, J774 to mast cell granules (MCG) led to the form ation of altered nuclear transcription factor proteins (NFκBx), which had faster electrophoretic mobility than the p50 homodimer of NF-κB, but retained comparable DNA binding capacity. Antibodies to N-terminal peptides of p50, p52, p65 or c-Rel supershifted only a fraction of NF-κBx. …”
Get full text

Association between non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio and telomere length: the NHANES 1999–2002 by Mingjie Liu, Chendong Wang, Bai Wei

“…TL was measured as the telomere-to-standard reference DNA ratio. Multivariate linear regression and smooth curve fitting were employed to assess the association between NHHR and TL.ResultsThe study population (mean age 45.1 ± 0.4 years, 48.9% male) was stratified into NHHR tertiles. …”
Get full text

中国黏膜黑色素瘤的临床特点及基因突变分析 by 黄复雪, 李丹丹, 文习之, 李婧婧, 祝保艳, 张晓实

“…最常见的基因突变为MYC拷贝数增加（36%，9/25），其次为KDR拷贝数增加，突变率为24%（6/25）。DNA损伤修复，细胞周期，PI3K-mTOR，生长因子受体，MAPK，免疫应答和WNT/NOTCH相关通路广泛存在突变，突变率分别为76%（19/25），72%（18/25），56%（14/25），60%（15/25），36%（9/25），28%（7/25），24%（6/25）。…”
Get full text

CRISPR/Cas Enzyme Catalysis in Liquid–Liquid Phase‐Separated Systems by Yaqin Zhang, Jianai Chen, Zhina Wu, Chenfei Zhao, Rui Wang, Zhiping Li, Jiasi Wang, Di Wang

“…Further analysis by increasing the concentration of the DNA reporter revealed that this phenomenon is not attributed to the slow diffusion of the reporter, and explained why Cas12a and Cas13a do not randomly cleave nucleic acids in the intracellular compartment. …”
Get full text

CRISPR-Cas9 in basic and translational aspects of cancer therapy by Maryam Samareh Salavatipour, Zahra Poursalehi, Negin Hosseini Rouzbahani, Sohaib Mohammadyar, Mohammad Vasei

“…CRISPR-Cas9 genome engineering has revolutionized this achievement by successful targeting the DNA molecule and editing its sequence. Since genomic changes are the basis of the birth and growth of many tumors, CRISPR-Cas9 method has been successfully applied to identify and manipulate the genes which are involved in initiating and driving some neoplastic processes. …”
Get full text

貉流产死胎分离出致病大肠杆菌 by 刘勃兴赵安奇刘畅王利丽张雪佳吴同垒高桂生史秋梅 张志强

“…对分离菌进行鉴定培养基培养、革兰氏染色、生化鉴定、16S rDNA基因测序分析和血清型检测试验，鉴定该菌；并进一步对分离菌进行致病性试验、药物敏感性试验、毒力基因和耐药基因检测。…”
Get full text

Bioinformation study of immune microenvironment characteristics of disulfidptosis-related subtypes in ovarian cancer and prognostic model construction by Ying Zhou, Yuhong Zhang, Yang Zhou, Yanzheng Gu, Youguo Chen, Juan Wang

“…Despite advancements in understanding DNA repair mechanisms and identifying novel therapeutic targets, additional strategies are still needed. …”
Get full text

Advancements in nanosensors for cancer detection by Dinesh Bhatia, Tania Acharjee, Monika Bhatia

“…Improved patient outcomes and early intervention are anticipated using the i-Genbox, a colorimetric sensor based on LAMP technology, and DNA-SWCNT-based sensors that further improve biomarker identification for gynecologic tumors.…”
Get full text

Surprising features of nuclear receptor interaction networks revealed by live-cell single-molecule imaging by Liza Dahal, Thomas GW Graham, Gina M Dailey, Alec Heckert, Robert Tjian, Xavier Darzacq

“…Type II nuclear receptors (T2NRs) require heterodimerization with a common partner, the retinoid X receptor (RXR), to bind cognate DNA recognition sites in chromatin. Based on previous biochemical and overexpression studies, binding of T2NRs to chromatin is proposed to be regulated by competition for a limiting pool of the core RXR subunit. …”
Get full text

Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus by Te-Ken Hsu, Yi-Yin Chen, Shiao-Wen Li, Hui-Yu Shih, Hsin-Yiu Chou, Jeff Chia-Kai Hsu, Han-Ching Wang, Li-Li Chen

“…Phage BP15 possessed a circular double-stranded DNA of 59,584 bp with a G + C content of 46.7% and lacked tRNA genes, virulence genes, and lysogeny genes. …”
Get full text

New taxa and a combination in Glomerales (Glomeromycota, Glomeromycetes) by Janusz Błaszkowski, Szymon Zubek, Paweł Milczarski, Ryszard Malinowski, Piotr Niezgoda, Bruno Tomio Goto

“…Dominikia indica strain 211 was previously characterized only by sequences of the 45S nuc rDNA region (= 18S, partial, ITS-1-5.8S-ITS2, 28S, partial) and the rpb1 gene (without any morphological data) that were deposited in GenBank under the incorrect name “Dominikia indica strain 211”. …”
Get full text