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4061
Hepatocyte Nuclear Factor 1 Beta Mutation-associated Newborn Onset of Glomerulocystic Kidney Disease: A Case Presentation
Published 2021-12-01“…A 10-year-old boy who was evaluated for bilateral cystic kidneys and chronic kidney disease from the newborn period was diagnosed with HNF1B-related glomerulocystic disease by DNA sequencing. The differential diagnosis of autosomal dominant polycystic kidney disease was a diagnostic pitfall. …”
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4062
日本血吸虫卵黄铁蛋白基因免疫在不同处理小鼠诱导免疫应答的观察
Published 2001-01-01“…【目的】研究日本血吸虫卵黄铁蛋白DNA 免疫在两种处理小鼠诱导的免疫应答及其保护作用。【方法】免疫小 鼠分两大组进行:实验Ⅰ 组小鼠有预感染, 实验Ⅱ 组小鼠没有预感染。…”
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4063
Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients
Published 2013-01-01“…Materials and Methods. DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy controls were undergoing bisulfite conversion and then analyzed for miR-124a promoter methylation using MSP assay. …”
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4064
Effects of Salmonella Typhimurium infection on intestinal flora and intestinal tissue arachidonic acid metabolism in Wenchang chickens
Published 2025-01-01“…Typhimurium using 16s rDNA sequencing and targeted metabolomics. The results showed that the levels of ARA metabolites were increased in the cecum tissue of Wenchang chickens after infection with S. …”
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4065
Polystyrene Nanoplastics Elicit Multiple Responses in Immune Cells of the <i>Eisenia fetida</i> (<i>Savigny</i>, 1826)
Published 2024-12-01“…., superoxide dismutase, catalase, and glutathione), and inducing lipid peroxidation and DNA oxidation. Additionally, assays on neutral red retention time, lysozyme activity, and Ca<sup>2</sup>⁺ levels demonstrated that NPs resulted in a loss of lysosomal membrane stability and a reduction in immune resistance. …”
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4066
Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction
Published 2024-10-01“…Diagnosis of T. lewisi is typically conducted using polymerase chain reaction (PCR), which amplifies target DNA using specific primers. One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). …”
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4067
Interaction of Herbal Compounds with Biological Targets: A Case Study with Berberine
Published 2012-01-01“…PANTHER analysis of berberine targets showed that the most classes of molecular functions include receptor binding, kinase activity, protein binding, transcription activity, DNA binding, and kinase regulator activity. Based on the biological process classification of in vitro berberine targets, those targets related to signal transduction, intracellular signalling cascade, cell surface receptor-linked signal transduction, cell motion, cell cycle control, immunity system process, and protein metabolic process are most frequently involved. …”
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4068
Innovative Technology Applications in Science Education: Digital Holography
Published 2021-02-01“…During the application process of the study, biodiversity, cells and divisions, DNA and genetic code, systems in our body topics, which are included in the science curriculum of the 5th, 6th, 7th and 8th grades were supported with digital holograms in a five-week period. …”
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4069
Prediction of the Sex-Associated Genomic Region in Tunas (Thunnus Fishes)
Published 2021-01-01“…In a previous study, a female genome of Pacific bluefin tuna (T. orientalis) was sequenced, and candidates for sex-associated DNA polymorphisms were identified by a genome-wide association study using resequencing data. …”
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4070
New Lung Cancer Panel for High-Throughput Targeted Resequencing
Published 2014-06-01“…Among the somatic mutations, up to 91% of single nucleotide polymorphisms from the two cancer samples were validated by DNA microarray-based genotyping. Our results demonstrated the feasibility of high-throughput mutation profiling with lung adenocarcinoma samples, and the profiling method can be used as a robust and effective protocol for somatic variant screening.…”
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4071
Periodic Distribution of a Putative Nucleosome Positioning Motif in Human, Nonhuman Primates, and Archaea: Mutual Information Analysis
Published 2013-01-01“…Recently, Trifonov's group proposed a 10-mer DNA motif YYYYYRRRRR as a solution of the long-standing problem of sequence-based nucleosome positioning. …”
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4072
Gene Profiles in the Early Stage of Neuronal Differentiation of Mouse Bone Marrow Stromal Cells Induced by Basic Fibroblast Growth Factor
Published 2020-01-01“…To identify the genes regulating neuronal differentiation, cDNA microarray analysis was conducted using mRNAs isolated from cells differentiated for different time periods (0, 4, 24, and 72 h) after bFGF treatment. …”
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4073
Root cap border cells as regulators of rhizosphere microbiota
Published 2025-01-01“…After separation of border cells from the root tip, transcription of primary metabolism genes decreases, whereas transcription of secondary metabolism genes as well as the synthesis and secretion of mucilage containing these metabolites along with extracellular DNA, proteoglycans and other substances increase. …”
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4074
Physiological value of intestinal microflora
Published 2011-09-01“…In the last years in clinical practice chemical methods, in particular gas chromatography (GC) in combination to mass spectrometry (GC-MS) are actively developed, the method of fluorescent DNA hybridization in situ (FISH) is applied, polymerase chain reaction (PCR-diagnostics) became quite widespread method of microorganisms assessment. …”
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4075
Safety Evaluation of the Novel Cocktail of Monoclonal Antibodies for Postexposure Prophylaxis in Category III Animal Exposures
Published 2024-12-01“…A novel cocktail of mAbs derived using recombinant DNA technology is presently available for usage. …”
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4076
Decoding vascular aging: implications for atherosclerosis progression and clinical intervention
Published 2024-08-01“…The promising therapeutic targets reviewed include the angiotensin (1–7)/MAS axis, the gut microbiome, histone deacetylases, DNA repair systems, noncoding RNAs, β3/dopamine adrenoceptors, senescence and inflammation checkpoints.…”
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4077
Prevalence of Drug Resistance and Associated Mutations in a Population of HIV-1+ Puerto Ricans: 2006–2010
Published 2012-01-01“…Samples from 2,500 patients from 2006–2010 were analyzed using the TruGene HIV-1 genotyping kit and the OpenGene DNA sequencing system. Results show that 58.8% of males and 65.3% of females had HIV-1 with resistance to at least one medication. …”
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4078
Anti-ribosomal P2: could it be a useful new criterion for patients with systemic lupus erythematosus?
Published 2025-01-01“…Abstract Background Systemic lupus erythematosus is a multisystem autoimmune disorder that displays numerous autoantibodies such as anti-nuclear, anti-Smith, anti-double-stranded DNA, and anti-ribosomal antibodies. Anti-ribosomal antibodies are detected in 15 to 40% of systemic lupus erythematosus patients. …”
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4079
Critical considerations and computational tools in plant genome editing
Published 2025-01-01“…Researchers may need to select appropriate software tools capable of analyzing post-editing detection of mutation events and other DNA sequence abnormalities to identify off-target effects. …”
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4080
A sequencing-based method for quantifying gene-deletion mutants of bacteria in the intracellular environment
Published 2025-01-01“…Based on standardized Illumina short-read sequencing of genomic DNA, the method eliminates the need for specific selective markers on each deletion mutant. …”
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