A Case of Visceral Leishmaniasis in a 4-Year-Old Child Living in Nonendemic Area Located in Suburbs of Dakar, Senegal by Magatte Ndiaye, Dienaba Fafa Cissé, Aicha Djigal, Aminata Sow, Souléye Lélo, Fatoumata Ly, Isaac A. Manga, Mame Ami Diouf, Doudou Sow, Oumar Gaye, Boubacar Camara, Babacar Faye

“…The PCR amplification of extracted DNA from the bone marrow aspiration using specific primers for VL (forward and reverse) confirmed that VL was responsible for the infection. …”
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Characterizing microbial communities and their correlation with genetic mutations in early-stage lung adenocarcinoma: implications for disease progression and therapeutic targets by Hao-Shuai Yang, Jin Zhang, Hong-Xiang Feng, Fei Qi, Fan-Jia Kong, Wei-Jie Zhu, Chao-Yang Liang, Zhen-Rong Zhang

“…Intratumoral microbiota may play a role in LUAD progression, but comprehensive stage-wise analysis is lacking.MethodsTumor and bronchoalveolar lavage fluid (BALF) samples from patients with AIS/MIA or IAC were collected for next-generation sequencing to characterize microbial diversity and composition. DNA extraction involved lysing samples with nuclease and protease, followed by homogenization and elution. …”
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应用双靶标CRISPR慢病毒载体在间充质干细胞中稳定敲除lncRNA DANCR基因 by 彭丹, 钟小敏

“…【方法】首先设计靶向DANCR的5’和3’端的sgRNA（single-guideRNA），转染293FT细胞，提取293FT细胞基因组DNA，验证靶向序列的有效性。然后通过gateway和酶切连接的方法，将分别靶向5’和3’端的两条有效sgRNA序列连接至同一慢病毒CRISPR载体。…”
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Cytoplasmic male sterility and prospects for its utilization in breeding, genetic studies and seed production of potato by I. N. Anisimova, T. A. Gavrilenko

“…The results of studies on developing DNA markers for identification of various potato cytoplasm types are presented.…”
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Purification, Characterization, and Biocatalytic and Antibiofilm Activity of a Novel Dextranase from Talaromyces sp. by Mahasen Mohamed Ahmed Ebaya, Mohammed El-Mowafy, Mohamed Mohamed Adel El-Sokkary, Ramadan Hassan

“…By using the standard ITS rDNA sequencing analysis, the isolated strain was identified as Talaromyces sp. …”
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Effect of Dietary Oils with Different Fatty Acid Compositions on Serum Lipid and Gut Microbiota of Rats by Tingwei Zhu, Yiming Kuai, Xingfeng Guo, Guanhao Bu, Chenxian Yang, Fusheng Chen

“…The serum metabolic index and liver histomorphology of rats were measured using an automatic biochemical analyzer and light microscope. Furthermore, 16S rDNA amplicon sequencing technology was used to analyze the gut microbiota. …”
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Expression of the chloroplast genome: modern concepts and experimental approaches by M. G. Siniauskaya, N. G. Danilenko, N. V. Lukhanina, A. M. Shymkevich, O. G. Davydenko

“…The chloroplast genome is relatively small, 100–120 genes, which encode less than 5 % of all proteins required for plastids to function. The cpDNA expression retains prokaryotic features, cotranscription in the operon, bacteria-like RNA polymerases and promoters, 70S ribosomes etc., also new characters appear such as uncoupling of transcription with translation, phage-type RNA polymerases, RNA editing, and splicing of primary transcripts. …”
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Epigenetic Methylation of Parathyroid CaR and VDR Promoters in Experimental Secondary Hyperparathyroidism by Jacob Hofman-Bang, Eva Gravesen, Klaus Olgaard, Ewa Lewin

“…Melting temperature profiling of CaR and VDR PCR products after bisulfite treatment of genomic DNA from rat parathyroids was performed. Real-time PCR measured expression of PTH, CaR, VDR, and klotho genes in vitro. …”
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Long Noncoding RNAs, Chromatin, and Development by Daniel P. Caley, Ryan C. Pink, Daniel Trujillano, David R. F. Carter

“…This differential expression is regulated at the epigenetic level by chromatin modifications, such as DNA and histone methylation, which interact with structural and enzymatic proteins, resulting in the activation or silencing of any given gene. …”
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Analysis of the Salivary Microbiome in Obstructive Sleep Apnea Syndrome Patients by Peizeng Jia, Jianyin Zou, Shankai Yin, Feng Chen, Hongliang Yi, Qian Zhang

“…Saliva was collected from 15 OSAS patients and nine healthy controls, and bacterial genomic DNA was extracted for 16S rRNA amplicon sequencing based on the Illumina platform. …”
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Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis by Alper Aytekin, Hikmet Kadakal, Deniz Mihcioglu, Turkan Gurer

“…Method In the present study, it was isolated RNA from both the tumor and non-tumor tissue of a total of 80 CRC patients and after synthesizing the cDNA, it was performed qRT-PCR to determine the expression levels of miR‑2861 and miR‑5011-5p. …”
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Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor by Soroush Dayani Najafabadi, Maryam Hasani Zonoozi, Roya Aliaskarian

“…According to the EzBioCloud database (eztaxon), the dominant bacterium identified through DNA extraction, PCR, and 16S-rRNA sequencing showed the highest similarity to Lysinibacillus Capsici PB300(T). …”
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Tropical fruit-derived Lactiplantibacillus as potential probiotic and antifungal agents against Fusarium oxysporum by R. Vasundaradevi, M. Sarvajith, S. Divyashree, N. Deepa, Premila N. Achar, M. Y. Sreenivasa

“…Two strains isolated from Ficus racemosa demonstrated significant antifungal activity against Fusarium oxysporum. 16S rDNA sequencing identified these strains as Lactiplantibacillus plantarum MYSVCF3 and Lpb. argentoratensis MYSVCF5. …”
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Breeding for postharvest physiological deterioration in cassava: problems and strategies by Wilton Mbinda, Asunta Mukami

“…Continuous improvements based on the new plant breeding technologies (genome editing, speeding breeding and RNA-dependent DNA methylation) in cassava and innovations in postharvest handling and storage of the storage roots are expected to provide sustainable solutions for PPD constraints and make cassava an important food security and nutrition and industrial crop.…”
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Clinical Report of a 17q12 Microdeletion with Additionally Unreported Clinical Features by Jennifer L. Roberts, Stephanie K. Gandomi, Melissa Parra, Ira Lu, Chia-Ling Gau, Majed Dasouki, Merlin G. Butler

“…Since syndrome-specific DNA sequencing studies failed to provide an explanation for this patient’s unusual habitus, we postulate that this case represents an expansion of the 17q12 microdeletion phenotype. …”
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The Critical Role of Bach2 in Shaping the Balance between CD4+ T Cell Subsets in Immune-Mediated Diseases by Lingyi Yang, Shuli Chen, Qiuyu Zhao, Ying Sun, Hong Nie

“…The transcription factor Bach2 which is predominantly expressed in B and T lymphocytes represses the expression of genes by forming heterodimers with small Maf and Batf proteins and binding to the corresponding sequence on the DNA. In this way, Bach2 serves as a highly conserved repressor which controls the terminal differentiation and maturation of both B and T lymphocytes. …”
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氨甲酰胆碱对多巴胺诱导的小脑颗粒细胞凋亡的保护机制 by 王雪敏, 邱鹏新, 苏兴文, 李文明, 黄守坚, 颜光美

“…【方法】在培养的小脑颗粒神经元建立多巴胺凋亡模型。用相差显微镜观察形态学, DNA 凝胶电泳和Hoechst 33258 核染色分析神经元凋亡, 细胞的存活率用二乙酸荧光素(FDA)染色法检测。…”
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DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts by Krzysztof Kuś, Loic Carrique, Tea Kecman, Marjorie Fournier, Sarah Sayed Hassanein, Ebru Aydin, Cornelia Kilchert, Jonathan M. Grimes, Lidia Vasiljeva

“…Xrn2 activity is stimulated by Spt5 to ensure efficient degradation of nascent RNA leading to Pol II dislodgement from DNA. Our results support a model where Xrn2 first forms a stable complex with the elongating Pol II to achieve its full activity in degrading nascent RNA revising the current ‘torpedo’ model of termination, which posits that RNA degradation precedes Xrn2 engagement with Pol II. …”
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Diagnostic Test to Identify Parkinson’s Disease from the Blood Sera of Chinese Population: A Cross-Sectional Study by Guangan Tong, Pingping Zhang, Wenbin Hu, Kun Zhang, Xianwen Chen

“…Serum miRNA profiling was carried out by synthesizing cDNA from the purified RNA and miRNA transcripts were determined by qRT-PCR using SYBR Green PremixScript. microRNA profiling strategy was employed for extracting the exosomal miRNAs from the exosomes. …”
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Gut Microbiota Secondary Metabolites: Key Roles in GI Tract Cancers and Infectious Diseases by Eman K. E. Anwer, Muhammad Ajagbe, Moustafa Sherif, Abobaker S. Musaibah, Shuaib Mahmoud, Ali ElBanbi, Anwar Abdelnaser

“…In gastrointestinal (GI) cancers such as colorectal, liver, and gastric cancer, microbial metabolites can drive tumorigenesis by promoting inflammation, DNA damage, and immune evasion. Conversely, these same metabolites hold therapeutic promise, potentially enhancing responses to chemotherapy and immunotherapy and even directly suppressing tumor growth. …”
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