PLK3 weakens antioxidant defense and inhibits proliferation of porcine Leydig cells under oxidative stress by Chujie Chen, Jinyan He, Weixian Huang, Dong Xu, Zhaohui Li, Anqi Yang

“…Protein-protein interaction (PPI) network analysis unveiled 15 key genes related to cell cycle and DNA replication, with polo-like kinase 3 (PLK3) exhibiting increased expression under OS. …”
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In-Situ Investigation of Copepod Predators of <i>Ichthyophthirius multifiliis</i> Theronts from Fish-Farming Pond by Lijun Wang, Bingwen Xi, Kai Chen, Jun Xie, Liangkun Pan

“…The natural predator of <i>I. multifiliis</i> theronts in fish-farming ponds were identified using fluorescent dye-labelled live theronts and quantitative PCR; meanwhile, the zooplankton community composition in the positive ponds of <i>I. multifiliis</i> detected by quantitative PCR were analyzed by eDNA metabarcoding assay. The results revealed predation on theronts by cyclopoid copepods, including <i>Cyclops vicinus</i>, <i>Thermocyclops taihokuensis</i>, <i>Cyclops</i> sp., <i>Thermocyclops</i> sp., <i>Eucyclops</i> sp., and <i>Mesocyclops</i> sp. from the in-situ predation aquatic ecosystem, and among these copepods, <i>C. vicinus</i> was identified as a natural dominant predator of <i>I. multifiliis</i>. …”
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Wilson Disease in a Turkish Population: Molecular Insights into an Old Disease with Reported and Novel Variants by Abdullatif Bakır, Vehap Topçu, Büşranur Çavdarlı

“…Methods: We investigated 49 patients from 46 families to determine the underlying molecular etiology of WD. DNA samples were extracted from peripheral blood. …”
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Whole Genome Sequencing Reveals Genetic Differences Between Symbiodiniaceae Populations Among Reproductively and Geographically Isolated Acropora Colonies in Western Australia by Sanna Y. Eriksson, Mikhail V. Matz, Peter D. Vize, Natalie L. Rosser

“…The conventional method of measuring Symbiodiniaceae diversity involving the ITS2 region of rDNA has several limitations, stemming from insufficient genetic resolution and the multi‐copy nature of the marker. …”
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Elaidic acid-induced intestinal barrier damage led to gut-liver axis derangement and triggered NLRP3 inflammasome in the liver of SD rats by Hui Liu, Xuenan Li, Lu Li, Yucai Li, Haiyang Yan, Yong Pang, Wenliang Li, Yuan Yuan

“…Our research investigated the effects of elaidic acid (EA), a typical TFA, on the gut microbiota to understand the underlying mechanisms of TFA-related chronic diseases. 16S rDNA gene sequencing on faecal samples from Sprague-Dawley rats were performed to explore the composition change of the gut microbiota by EA gavage for 4 weeks. …”
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Construction and Evaluation of a Subcutaneous Splenic Injection Port for Serial Intraportal Vein Cell Delivery in Murine Disease Models by Toshio Miki, Chika Takano, Irving M. Garcia, Brendan H. Grubbs

“…Cell distribution was analyzed by quantifying human DNA using human Alu-specific primers. About 50% of the transplanted cells were located homogeneously in the liver one hour after the splenic port injection. …”
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Widespread mutagenesis and chromosomal instability shape somatic genomes in systemic sclerosis by Sriram Vijayraghavan, Thomas Blouin, James McCollum, Latarsha Porcher, François Virard, Jiri Zavadil, Carol Feghali-Bostwick, Natalie Saini

“…We hypothesize that the inflammation and immune response associated with systemic sclerosis can trigger DNA damage, leading to elevated somatic mutagenesis, a hallmark of pre-cancerous tissues. …”
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Deciphering mixed infections by plant RNA virus and reconstructing complete genomes simultaneously present within-host. by Martine Bangratz, Aurore Comte, Estelle Billard, Abdoul Kader Guigma, Guillaume Gandolfi, Abalo Itolou Kassankogno, Drissa Sérémé, Nils Poulicard, Charlotte Tollenaere

“…To this end, we tested different sequencing strategies, and we finally combined direct cDNA ONT (Oxford Nanopore Technology) sequencing with the bioinformatics tool RVhaplo. …”
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Comprehensive Analysis of Breast Cancer Cell Lines: Genome-wide Insights from ChIP-seq Analysis by Tanishq Sahu, Ruchi Yadav

“…Chromatin immunoprecipitation coupled with sequencing (ChIP-seq) is an important technology to identify the genome-wide location of DNA-binding proteins such as histones proteins, transcription factors, RNA polymerase, or any protein of interest. …”
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Molecular Mechanisms Contributing Bacterial Infections to the Incidence of Various Types of Cancer by Salah A. Sheweita, Awad S. Alsamghan

“…These microorganisms produce a high level of N-nitrosamines which are metabolically activated leading to the generation of alkylating agents that damage DNA and other macromolecules. It is concluded that a certain bacterium is linked with induction of a specific type of cancer via different molecular and biochemical mechanisms as discussed in the text in details. …”
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Insights Into Genetic Variations of the OCT1 Gene in Metformin Poor Responders Among Bangladeshi Type 2 Diabetic Patients by Rokeya Begum, Arindita Das, Md. Jahangir Alam, Gazi Nurun Nahar Sultana

“…Clinical features of 56 Bangladeshi T2DM patients (who showed altered response to metformin) were analyzed, and genomic DNA was extracted from their blood samples. Subsequently, the entire exons (1–11), along with flanking introns of the OCT1 gene were amplified and sequenced. …”
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Three MYO15A Mutations Identified in One Chinese Family with Autosomal Recessive Nonsyndromic Hearing Loss by Fengguo Zhang, Lei Xu, Yun Xiao, Jianfeng Li, Xiaohui Bai, Haibo Wang

“…The goal of this research is to explore the genetic cause of a Chinese deafness pedigree who was excluded of GJB2, SLC26A4, or MtDNA12SrRNA variants. Three variants, c.3971C>A (p.A1324D), c.4011insA (p.Q1337Qfs∗22), and c.9690+1G>A, in the MYO15A gene were identified by targeted capture sequencing and Sanger sequencing, and the first two of them were novel. …”
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Antibiotic resistance gene occurrence in poultry farms in northeast Brazil by Henrique Francisco de Almeida, Paulo Ricardo Conceição Marques Trindade, César Roberto Viana Teixeira, Claudson Oliveira Brito, Silvio Santana Dolabella, Sona Jain, Maíra Pompeu Martins, Ana Andréa Barbosa

“…A total of 14 chicken litter samples were collected from twelve farms and subjected to total DNA extraction. The presence of ARGs in the obtained material was tested by Polymerase Chain Reaction (PCR) using primers for selected ARGs. …”
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Progressive Multifocal Leukoencephalopathy in a Multiple Sclerosis Patient Diagnosed after Switching from Natalizumab to Fingolimod by Tim Sinnecker, Jalal Othman, Marc Kühl, Imke Metz, Thoralf Niendorf, Annett Kunkel, Friedemann Paul, Jens Wuerfel, Juergen Faiss

“…We report a MS patient in whom PML was highly suspected solely based on MRI findings after switching from NTZ to fingolimod despite repeatedly negative (ultrasensitive) polymerase chain reaction (PCR) testing for JC virus DNA in cerebrospinal fluid. The PML diagnosis was histopathologically confirmed by brain biopsy. …”
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FBXL16 Promotes Endometrial Progesterone Resistance via PP2AB55α/Cyclin D1 Axis in Ishikawa by Haoen Liu, Li Han, Liyan Zhong, Xiaodan Zhuang, Yan Peng

“…MPA tolerance of endometrial cancer cells was inhibited by knockdown of FBXL16 in DNA content assessment, CCK-8, and colony formation. …”
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Structural Properties of G,T-Parallel Duplexes by Anna Aviñó, Elena Cubero, Raimundo Gargallo, Carlos González, Modesto Orozco, Ramon Eritja

“…The structure of G,T-parallel-stranded duplexes of DNA carrying similar amounts of adenine and guanine residues is studied by means of molecular dynamics (MD) simulations and UV- and CD spectroscopies. …”
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Mechanisms of transcriptional regulation of ecdysone response by M. Yu. Mazina, N. E. Vorobyeva

“…The same genes can be activated or repressed under the influence of ecdysone in different tissues. Likely, certain DNA-binding transcription factors that are involved in the ecdysonedependent response together with the EcR/Usp heterodimer are responsible for cell-type specificity. …”
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Evolutionarily Developed Alternatively Spliced Exons Containing Translation Initiation Sites by Jun-ichi Takeda, Takaaki Okamoto, Akio Masuda

“…The splicing of 5UC-ASEs is under the repressive control of MATR3, a DNA/RNA-binding protein associated with neurodegeneration, and is distinctly regulated particularly in the human brain, muscle, and testis. …”
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Progress in plant genome sequencing: research directions by M. K. Bragina, D. A. Afonnikov, E. A. Salina

“…A comparison of the methods of targeted sequencing based on the use of the reference DNA sequence is accomplished.…”
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Inferring Tripartite Associations of Vector-Borne Plant Pathogens Using a Next-Generation Sequencing Approach by Ava M. Gabrys, Christopher H. Dietrich, Valeria Trivellone

“…Here, we used anchored hybrid enrichment (AHE) to recover the DNA of five plant genes (<i>rbcL</i>, <i>matK</i>, <i>ITS1</i>, <i>ITS2</i>, and <i>trnH-psbA</i>) in 58 phloem-feeding leafhoppers from around the world that had previously tested positive for phytoplasma infection. …”
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