Description and characterization of pneumococcal community acquired pneumonia (CAP) among radiologically confirmed CAP in outpatients by Josselin Le Bel, Juliette Pinot, Toni Alfaiate, Marie Ecollan, Fanny Cussac, Raphaël Pecqueur, Marie-Pierre Revel, Cyrille Vartanian, Emmanuelle Varon, Christian Theilacker, Xavier Duval, Henri Partouche, Cédric Laouénan, Serge Gilberg

“…To identify other pathogens, next to cultures, nasopharyngeal multiplex PCR was performed. …”
Get full text

Screening of Lignocellulose-Degrading Superior Mushroom Strains and Determination of Their CMCase and Laccase Activity by Li Fen, Zhu Xuwei, Li Nanyi, Zhang Puyu, Zhang Shuang, Zhao Xue, Li Pengju, Zhu Qichao, Lin Haiping

“…When cultivated in guaiacol culture medium to detect their abilities to secrete laccase, Hericium erinaceus showed the highest ability with the largest reddish brown circles of 4.330 cm. …”
Get full text

Impedance mapping with high-density microelectrode array chips reveals dynamic heterogeneity of in vitro epithelial barriers by Alessandra Venz, Bastien Duckert, Liesbet Lagae, Saeedeh Ebrahimi Takalloo, Dries Braeken

“…Abstract Epithelial tissues in vitro undergo dynamic changes while differentiating heterogeneously on the culture substrate. This gives rise to diverse cellular arrangements which are undistinguished by conventional analysis approaches, such as transepithelial electrical resistance measurement or permeability assays. …”
Get full text

Development and evaluation of multiplex real-time PCR for rapid identifying major pathogenic mycobacteria from pulmonary and extrapulmonary clinical samples in eastern China by Tingting Fang, Lijun Peng, Tingting Yang, Qingshan Cai, Huanyu Li, Hao Li, Long Cai

“…For NTM detection in pulmonary samples, the sensitivity was slightly lower than Culture (P > 0.05) but higher than CapitalBio RT-PCR (P < 0.05). …”
Get full text

Pain in the Prehospital Setting in Rwanda: Results of a Mixed-Methods Quality Improvement Project by A. Rosenberg, E. Uwitonze, M. Dworkin, J. P. D. Guidry, T. Cyuzuzo, D. Banerjee, K. McIntyre, K. Carlyle, J. M. Uwitonze, I. Kabagema, T. Dushime, S. Jayaraman

Get full text

Dietary γ-Aminobutyric Acid Promotes Growth and Immune System Performance and Improves Erythropoiesis and Angiogenesis in Gibel Carp (<i>Carassius auratus gibelio</i>) by Xinlan Bai, Lu Zhang, Hualiang Liang, Dongyu Huang, Mingchun Ren, Haifeng Mi

“…This experiment aimed to investigate the effect of dietary supplementation of γ-aminobutyric acid (GABA) on the growth performance, immune response, and oxygen-transport-related factors of Gibel carp (<i>Carassius auratus gibelio</i>). An eight-week culturing experiment was designed with five experimental diets, with the actual GABA content being 368 mg/kg (G1, control group), 449 mg/kg (G2), 527 mg/kg (G3), 602 mg/kg (G4), and 675 mg/kg (G5). …”
Get full text

Effect of Different Medium on Survival Rate and Growth of Chironomus sp. Larvae by . Widanarni, D.D. Mailana, O. Carman

“…That problem  may be overcome by culturing blood worm in appropriate culture medium.  …”
Get full text

Historical origin and development of politically motivated criminal offences: Political delicts by Matić Goran D.

“…One of the most important achievements of the European legal culture and thought from humanism and the Renaissance to the most recent times is the establishment of the concept and legal institute of political delict, as a privileged delict - criminal offence regulated by law, in terms of general and legal culture, despite the fact that it has been limited mainly to verbal political actions in democratic states. …”
Get full text

Effect of Pien Tze Huang on Lymphangiogenesis Mediated by LncRNA-ANRIL in Colorectal Cancer by Yao LU, Min ZHANG, Jie LIU, Qianqian MAO, Zhiyun CAO, Jiumao LIN

“…Treat HCT-116 cells with Si-NC, Si-ANRIL and Si-NC+PZH(0.25 mg/mL) respectively and collect cell culture supernatants (Tumor culture supernatants, TSNs) for theculture of human lymphatic endothelium cell (HLEC). …”
Get full text

Fusobacterium nucleatum promotes colorectal cancer liver metastasis via miR-5692a/IL-8 axis by inducing epithelial-mesenchymal transition by Yulong Yu, Han Yin, Bili Wu, Weiheng Zhao, Yuan Wang, Aifeina Aili, Mu Yang, Qianqian Yu, Xianglin Yuan

“…Results The metastasis ability of CRC cells was significantly enhanced after co-culture with F. nucleatum in vitro. In the mouse model, F. nucleatum also promoted the development of liver metastasis in CRC. …”
Get full text

In Vitro Antagonistic Effect of Lactic Acid Bacteria Isolated from Fermented Beverage and Finfish on Pathogenic and Foodborne Pathogenic Microorganism in Ethiopia by Fitsum Dejene, Belayneh Regasa Dadi, Dagimawie Tadesse

“…Then, cell-free supernatant (CFS) was prepared from MRS culture and used in an antimicrobial assay that was performed by agar diffusion method. …”
Get full text

Optimizing in vitro spherulation cues in the fungal pathogen Coccidioides by Christina M. Homer, Elena Ochoa, Mark Voorhies, Anita Sil

“…One of the foremost technical challenges has been reliably growing spherules in culture without the formation of contaminating hyphae and consistently inducing endospore release from spherules. …”
Get full text

Effect of meta-Topolin on morphological, physiochemical, and molecular dynamics during in vitro regeneration of Salix tetrasperma Roxb. by Zubair Altaf Reshi, Fohad Mabood Husain, Mehrun Nisha Khanam, Saad Bin Javed

“…Among individual cytokinin treatments, 5.0 µM mT resulted highest response of 92% with maximum shoot number (11.6 ± 0.08) per explant and shoot length (4.5 ± 0.22 cm) after 12 weeks of culture. However, synergistic treatment of mT (5.0 µM) and NAA (0.5 µM) further improved proliferation with (21.3 ± 0.40) shoots per explant and (6.9 ± 0.13 cm) shoot length in 96% cultures after 12 weeks of incubation. …”
Get full text

Mini Worldlit: A Dataset of Contemporary Fiction from 13 Countries, Nine Languages, and Five Continents by Andrew Piper, David Bamman, Christina Han, Jens Bjerring-Hansen, Hoyt Long, Itay Marienberg-Milikowsky, Tom McEnaney, Mathias Iroro Orhero, Emrah Peksoy, Pallavi Rastogi, Sebastian Rasmussen, Roel Smeets, Alexandra Stuart, Mads Rosendahl Thomsen

“…The dataset provides a foundation for future comparative studies of global literary cultures, offering a template for cross-cultural sampling. …”
Get full text

Surface functionalization of microscaffolds produced by high-resolution 3D printing: A new layer of freedom by Oliver Kopinski-Grünwald, Stephan Schandl, Jegor Gusev, Ourania Evangelia Chamalaki, Aleksandr Ovsianikov

“…Previously, we demonstrated the successful differentiation of such building blocks, produced from immortalized human adipose-derived stem cells, towards different phenotypes, and the possibility of creating macro-sized tissue-like constructs in vitro. The culture of cells in vitro depends on the supply of various nutrients and biomolecules, such as growth factors, usually supplemented in the culture medium. …”
Get full text

Incorporation of anthocyanin into zein nanofibrous films by electrospinning: Structural characterization, functional properties, and ammonia color-responsiveness by Jun Wang, Wenpeng Zhao, Dongli Qin, Hongyan Shan, Xiaoman Zhu, Lilan Chen, Ying Liu, Songnan Li

Get full text

Evaluation of developmental competence of Sus scrofa domesticus (L.) oocyte-cumulus complexes after intra- and extraovarian vitrification by T. I. Kuzmina, I. V. Chistyakova

“…The methods of fertilization and embryo culture are presented in the guidelines developed by us. …”
Get full text

Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins by Mark R. Dalman, W. Brian Simison, Danny Nielsen, Sabana Bhatta, Noor Ramahi, Clair Yee, Dipendra Thapaliya, Jhalka Kadariya, Shanice Cheatham, Hailee Olson, Tara C. Smith

“…Microbiome composition was most similar in twins’ nares that were S. aureus culture-positive concordant, whereas twins that were culture-negative concordant had the most similarity in the oropharynx. …”
Get full text

Correlation Between <i>Cryptococcus</i> Infection and the Nasal Mycobiota in a Population of Free-Ranging Koalas (<i>Phascolarctos cinereus</i>) in New South Wales, Australia by Andrew S. McPherson, Sophie L. Haworth, Alex Kan, Luisa Monteiro de Miranda, Mark B. Krockenberger

“…Of the 47 animals tested, 6.4% were culture-positive only, 4.3% were seropositive only, and 2.1% were culture- and seropositive. …”
Get full text

Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells by Meng Sun, Linxiao Sun, Chongchu Huang, Bi-cheng Chen, Zhenxu Zhou

“…ADMSCs and peritoneal macrophages were collected from C57BL/6 mice. The culture medium (CM) from ADMSCs (24 hours cultured) was collected. …”
Get full text