Interleukin-12 Inhibits Tumor Growth and Metastasis Promoted by Tumor-Associated Mesenchymal Stem Cells in Triple-Negative Breast Cancer by Babak Jahangiri, Zahra-Soheila Soheili, Elahe Asadollahi, Mehdi Shamsara, Vahid Shariati, Alireza Zomorodipour

“…Objective: The aim of this study was to understand the interactions between tumor-associated mesenchymal stemcells (TA-MSCs) and triple-negative breast cancer (TNBC) cells, which appear to be necessary for developing effectivetherapies.Materials and Methods: In this experimental study, MDA-MB-231 and 4T1 TNBC cells were co-cultured with bonemarrow-derived MSCs, and TA-MSCs conditioned media (CM) were collected. …”
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S’unir au Prophète. L’expérience matérielle, esthétique et dévotionnelle du Dalā’il al-Khayrāt au Maroc. Approches codicologique et anthropologique by Hiba Abid, Anouk Cohen

“…To this end, the article draws on codicological and anthropological approaches to the book to examine how the materiality of the object informs the cultural practices that are attached to it, and the material and aesthetic experience that it supports, in order to unite with the Prophet, and also reconfigures these.…”
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Healthy aging in frail older adults: Active aging project of a national survey by Jisu Seo, Kyungok Joo, Yuelin Li, Nayoung Kim, Eunna Oh, Lkhagvajav Gansukh, Rhayun Song

“…Further, we need to explore diverse social and cultural activities associated with healthy aging in this population to expand the definition of active aging.…”
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Addressing racial and ethnic disparities in premature exits from permanent supportive housing among residents with substance use disorders by Talia J. Panadero, Sonya Gabrielian, Marissa J. Seamans, Lillian Gelberg, Jack Tsai, Taylor Harris

“…Conclusions These findings suggest relationships between SUDs and negative PSH exits differ between racial/ethnic groups and suggest there may be value in culturally specific tailoring and implementation of SUD services for these subgroups.…”
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A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections by Amber De Bleeckere, Frits van Charante, Thibault Debord, Stien Vandendriessche, Michiel De Cock, Marte Verstraete, Fabien Lamret, Bram Lories, Jerina Boelens, Fany Reffuveille, Hans P. Steenackers, Tom Coenye

“…The SSF2 medium allowed isolates to grow and form biofilm-like aggregates varying in size and shape between different species. For most isolates cultured in SSF2, a reduced susceptibility to the tested antibiotics was observed when compared to susceptibility data obtained in general media. …”
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hUC-MSC preserves erectile function by restoring mitochondrial mass of penile smooth muscle cells in a rat model of cavernous nerve injury via SIRT1/PGC-1a/TFAM signaling by Mengbo Yang, Xinda Chen, Ming Zhang, Xiaolin Zhang, Dongdong Xiao, Huiming Xu, Mujun Lu

“…In vitro, CCSMCs underwent hypoxia and were then co-cultured with ADSC or hUC-MSC using a transwell system. …”
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Metabolomics and Transcriptomics Reveal the Effects of Different Fermentation Times on Antioxidant Activities of <i>Ophiocordyceps sinensis</i> by Min He, Tao Wang, Chuyu Tang, Mengjun Xiao, Xiaojian Pu, Jianzhao Qi, Yuling Li, Xiuzhang Li

“…<i>Ophiocordyceps sinensis</i> is a fungus that is cultured through fermentation from wild Chinese cordyceps. …”
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The methyl-CpG binding domain 2 regulates peptidylarginine deiminase 4 expression and promotes neutrophil extracellular trap formation via the Janus kinase 2 signaling pathway in e... by Biao Peng, Mu-Yun Yan, Yun-Rong Chen, Fei Sun, Xu-Dong Xiang, Da Liu

“…HL-60 cells were differentiated into neutrophil-like cells by culturing in a medium containing dimethyl sulfoxide and then stimulated with LPS. …”
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In Vitro Screening and Transfection Concentration Optimization of Cynomolgus Monkey IκBα-siRNA by Zhaoxing Ou, Rui Zeng, Yifan Lin, Si Zhang, Mohammad Alzogool, Peng Zeng, Yuqing Lan

“…They were transfected into primarily cultured cynomolgus monkey CM cells and TM cells. The mRNA and protein levels of IκBα were examined by using real-time quantitative polymerase chain reaction (real-time PCR) and western blot to screen a pair of candidate valid sequences with the highest inhibitory rate. …”
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Divergent paths of mammary gland involution: unveiling the cellular dynamics in abruptly and gradually involuted mouse models by Sarmila Majumder, Sanjay Mishra, Neelam Shinde, Maria C. Cuitino, Morgan Bauer, Dinesh Ahirwar, Mustafa M. Basree, Vijaya Bharti, Kate Ormiston, Resham Mawalkar, Sara Alsammerai, Gautam Sarathy, Anna E. Vilgelm, Xiaoli Zhang, Ramesh K. Ganju, Bhuvaneswari Ramaswamy

“…Immune cell changes were investigated by flow cytometry, cytokine array and multiplex immunofluorescence. 3D-organoid cultures were used for in vitro assay of luminal progenitor cells. …”
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Principles, barriers and enablers to agroecological animal production systems: a qualitative approach based on five case studies by B. Dumont, C. Barlagne, P. Cassart, J.E. Duval, A. Fanchone, J-L. Gourdine, O. Huguenin-Elie, Y. Kazakova, J. Klötzli, A. Lüscher, E. Oteros-Rozas, D. Pomies, M.G. Rivera Ferre, W.A.H. Rossing, V. Stefanova, A. Swartebroeckx, C. Zagaria

“…Most agroecological systems benefited from a positive image among citizens and consumers, but cultural barriers resulted from change in product characteristics, e.g. veal meat colour in dairy system that promote long-lasting cow-calf contact. …”
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Molecular and cellular aspects of the impact of secondary metabolites from common barberry and hybrid goat’s rue on the HeLa cell line by E. Yu. Zlatnik, Ya. S. Enin, O. N. Burov, E. S. Bondarenko, A. B. Sagakyants, D. S. Kutilin, Yu. V. Dzigunova, O. G. Ishonina, E. V. Shalashnaya, N. D. Ushakova

“…Subsequently, an experiment was conducted using cultural and molecular methods on the HeLa cell line in three repetitions for each test compound; after incubation with them, the numbers of dead cells were counted on the automated NanoEnTek JuliFl counter, and the numbers of cells in apoptosis were measured by flow cytometry on the BD analyzer FACS Canto II. …”
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FAM134B-mediated endoplasmic reticulum autophagy protects against cisplatin-induced spiral ganglion neuron damage by Fan Wang, Yue Xu, Yajie Wang, Qian Liu, Yanan Li, Weiwei Zhang, Huiming Nong, Junhong Zhang, Hao Zhao, Huaqian Yang, Lingchuan Guo, Jianfeng Li, Jianfeng Li, Hong Li, Qianqian Yang

“…The present study was designed to determine whether FAM134B is expressed in SGNs of C57BL/6 mice and, if so, to explore the potential function of FAM134B in Cis-induced SGN damage in vitro.MethodsMiddle turns of neonatal murine cochleae were cultured and treated with 30 μM Cis in vitro. The distribution of FAM134B, morphological changes of SGNs, and the colocalization of ER segments with lysosomes were measured by immunofluorescence (IF). …”
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Thioredoxin-interacting protein (TXNIP) inhibition promotes retinal ganglion cell survival and facilitates M1-like microglial transformation via the PI3K/Akt pathway in glaucoma by Junjue Chen, Huimin Zhong, Bingqiao Shen, Huan Yu, Yang Zhang, Ruiqi Han, Ping Huang, Shouyue Huang, Yisheng Zhong

“…Microglia high-pressure cultured model was constructed. Western blot, RT-PCR and immunofluorescence were used to investigate the proinflammatory cytokines secretion, glucose uptake and phenotype transformation in wild type, TXNIP knockout and overexpressed microglia combined with IL-17A treatment. …”
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Regulation of LPS-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells via TLR4-Mediated NF-κB and MAPK Signaling Pathways by Lactoferrin by Kai Zhang, Ruizhen Zhang, Yuanyuan Zhang, Min Zhang, Hong Su, Feifei Zhao, Daqing Wang, Guifang Cao, Yong Zhang

“…This study used bovine mammary epithelial cells (BMECs) cultured in vitro as the research model. An inflammatory injury model was established by stimulating BMECs with LPS to investigate whether LF at different concentrations (10, 50, 100, and 200 μg·mL⁻¹) could inhibit the inflammatory response before and after the onset of inflammation. …”
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Age-associated microglial transcriptome leads to diminished immunogenicity and dysregulation of MCT4 and P2RY12/P2RY13 related functions by Martin Škandík, Lara Friess, Guillermo Vázquez-Cabrera, Lily Keane, Kathleen Grabert, Mireia Cruz De los Santos, Mercedes Posada-Pérez, Austeja Baleviciute, Mathilde Cheray, Bertrand Joseph

“…Through the integration of in vitro cultured BV-2 microglia with primary microglia datasets, we identify critical molecular determinants of microglial cellular aging confirmed in human-aged brain tissue. …”
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Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa by Yaw Adjei Anane, Teke Apalata, Sandeep Vasaikar, Grace Emily Okuthe, Sandile Songca

“…A total of 100 nonduplicate multidrug-resistant A. baumannii strains were cultured from clinical samples obtained from healthcare facilities in the O. …”
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Intratumoral BO-112 in combination with radiotherapy synergizes to achieve CD8 T-cell-mediated local tumor control by Helena Escuin-Ordinas, Ignacio Melero, Marisol Quintero, Jose Luis Perez-Gracia, Maite Alvarez, Maria E Rodriguez-Ruiz, Miguel F Sanmamed, Paloma Sanchez-Mateos, Inmaculada Rodríguez López, Irantzu Serrano-Mendioroz, Celia Barrio-Alonso, Eneko Garate-Soraluze, Victor Diaz Pascual, Leire Arbea Moreno

“…Depletions of immune cell populations and knockout mice for the IFNAR and BATF-3 genes were used to delineate the immune system requirements for efficacy.Results In cultures of TS/A breast cancer cells, the combination of irradiation and BO-112 showed more prominent features of immunogenic tumor cell death in terms of calreticulin exposure. …”
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Structure and Expression of Two Sex-Related dmrt Genes in Oxyeleotris lineolata by Jiajia FAN, Dongmei MA, Huaping ZHU, Minghui LIN, Zaixuan ZHONG, Yuanyuan TIAN

“…Oxyeleotris lineolata is a valuable economic fish cultured in Australia and countries of Southern Asia. …”
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Stimulation of DC-CIK with PADI4 Protein Can Significantly Elevate the Therapeutic Efficiency in Esophageal Cancer by Chunyan Liu, Yingying Zheng, Junyi Tang, Dawei Wang, Zhenshen Ma, Shutong Li, Xiaotian Chang

“…The cytotoxic effect of DC-CIK cells on cultured ECA-109 cells was determined using CCK8 assays. …”
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