CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…Background. Organoid cultures of human pancreatic ductal adenocarcinoma (PDAC) have become a promising tool for tumor subtyping and individualized chemosensitivity testing. …”
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Evidence that the domesticated fungus Leucoagaricus gongylophorus recycles its cytoplasmic contents as nutritional rewards to feed its leafcutter ant farmers by Caio Ambrosio Leal-Dutra, Lok Man Yuen, Bruno Augusto Maciel Guedes, Marta Contreras-Serrano, Pedro Elias Marques, Jonathan Zvi Shik

“…We next confirmed that autophagy plays a critical role in gongylidium formation both: (1) in vitro as gongylidium suppression occurred when isolated fungal cultures were grown on media with autophagy inhibitors, and (2) in silico as differential transcript expression (RNA-seq) analyses showed upregulation of multiple autophagy gene isoforms in gongylidia relative to undifferentiated hyphae. …”
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Guia Beabá do Câncer: Validação de Livro Educativo para Crianças, Famílias e Profissionais de Saúde by Simone Lehwess Mozzilli, Pedro Emílio Gomes Prates, Isadora Cardoso Salles Fila Pecenin, Lucas Thiago Pereira da Silva, Marina de Góes Salvetti

“…Método: Estudo metodológico de validação de conteúdo utilizando o método Suitability Assessment of Materials (SAM) para avaliar a adequação do material em seis categorias (conteúdo, linguagem, ilustração, apresentação, motivação e adaptação cultural) e indicadores da experiência do usuário. …”
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Extended spectrum betalactamase and Carbapenemase producing gram negative bacteria from healthcare workers gowns at Debre Berhan Comprehensive Specialized Hospital, Ethiopia by Sisay Gebrish Sharew, Daniel Gebretsadik Weldehanna, Demissew Shenkute Gebreyes, Tsegahun Asfaw Abebe, Agumas Shibabaw

“…A total of 226 swab samples were collected from gowns of HCWs and cultured on MacConkey agar. Bacterial identifications were done by standard biochemical tests. …”
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Protection of the Peritoneal Membrane by Peritoneal Dialysis Effluent-Derived Mesenchymal Stromal Cells in a Rat Model of Chronic Peritoneal Dialysis by Lan Zhou, Ming Zong, Qiunong Guan, Gerald da Roza, Hao Wang, Hualin Qi, Caigan Du

“…In vitro incubation with pMSC-conditioned medium prevented cell death in cultured human peritoneal mesothelial cells (HPMCs) and downregulated proinflammatory (i.e., CXCL6, NOS2, IL1RN, CCL5, and NR3C1) while upregulated anti-inflammatory (i.e., CCR1, CCR4, IL9, and IL-10) gene expression in activated THP1 cells. …”
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NLRX1 limits inflammatory neurodegeneration in the anterior visual pathway by Alexander J. Gill, Matthew D. Smith, Danny Galleguillos, Thomas Garton, Jackson W. Mace, Sachin P. Gadani, Swati Kumar, Aayush Pokharel, Krista Solem, Saahith Potluri, Omar Hussein, Giuliana Sardi Rogines, Arihant Singh, Annatje Clark, Peter A. Calabresi, Marjan Gharagozloo

“…The novel pharmacologic NLRX1 activators NX-13 and LABP-66 decreased LPS-mediated gene expression of inflammatory cytokines and chemokines in mixed glial cultures. Moreover, treating EAE mice with oral LABP-66, compared to vehicle, after the onset of paralysis resulted in less anterior visual pathway neurodegeneration. …”
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HOTSSea v1: a NEMO-based physical Hindcast of the Salish Sea (1980–2018) supporting ecosystem model development by G. Oldford, G. Oldford, T. Jarníková, V. Christensen, M. Dunphy

“…<p>Decadal-scale oceanographic, environmental, and ecological changes have been reported in the Salish Sea, an ecologically productive inland sea in the northeast Pacific that supports the economies and cultures of millions of people. However, there are substantial data gaps related to physical water properties that make it difficult to evaluate trends and the pathways of effects between physical ocean water properties and the productivity of marine ecosystems. …”
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Creating a family health history interview protocol for use with undergraduate health professional students: A scoping review by Ronica N. Rooks, Cassandra D. Ford, Jenna Bennett, Tyrone Braxton

“…Results: Protocol suggestions included having appropriate settings and preparation to conduct interviews with questions on socio-demographics, cultural and family relationship dynamics, health behaviors, and acute and chronic condition questions for family members. …”
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miR-1233-3p Inhibits Angiopoietin-1-Induced Endothelial Cell Survival, Migration, and Differentiation by Veronica Sanchez, Sharon Harel, Anas Khalid Sa’ub, Dominique Mayaki, Sabah N. A. Hussain

“…This study highlights that miR-1233-3p exerts inhibitory effects on Ang-1-induced survival, migration, and the differentiation of cultured ECs.…”
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Optimization of a micro-scale air–liquid-interface model of human proximal airway epithelium for moderate throughput drug screening for SARS-CoV-2 by Chandani Sen, Tammy M. Rickabaugh, Arjit Vijey Jeyachandran, Constance Yuen, Maisam Ghannam, Abdo Durra, Adam Aziz, Kristen Castillo, Gustavo Garcia, Arunima Purkayastha, Brandon Han, Felix W. Boulton, Eugene Chekler, Robert Garces, Karen C. Wolff, Laura Riva, Melanie G. Kirkpatrick, Amal Gebara-Lamb, Case W. McNamara, Ulrich A. K. Betz, Vaithilingaraja Arumugaswami, Robert Damoiseaux, Brigitte N. Gomperts

“…As a result, most of the drug screening assays for respiratory viruses are performed on commercial cell line-based 2D cultures that provide limited translational ability. …”
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To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway by Jia-Rong Gao, Xiu-Juan Qin, Zhao-Hui Fang, Li-Shan, Li-Ping Han, Hui-Jiang, Ming-Fei Guo, Nan-Nan Jiang

“…In vitro cells were cultured with 30 mM glucose for 24 h. RT-QPCR was used to detect the mRNA expression of endothelial markers PTEN, PI3K, Akt, and VEGF. …”
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The BET inhibitor JQ1 targets fat metabolism and counteracts obesity by Claudia Fornelli, Alessia Sofia Cento, Lorenzo Nevi, Raffaella Mastrocola, Gustavo Ferreira Alves, Giuseppina Caretti, Massimo Collino, Fabio Penna

“…A direct JQ1 lipolytic effect was demonstrated on mature adipocyte cultures. JQ1-mediated loss of adipose tissue mass was not associated with systemic inflammation or with lipid accumulation in muscle and liver. …”
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Genomic characterization of three marine fungi, including Emericellopsis atlantica sp. nov. with signatures of a generalist lifestyle and marine biomass degradation by Ole Christian Hagestad, Lingwei Hou, Jeanette H. Andersen, Espen H. Hansen, Bjørn Altermark, Chun Li, Eric Kuhnert, Russell J. Cox, Pedro W. Crous, Joseph W. Spatafora, Kathleen Lail, Mojgan Amirebrahimi, Anna Lipzen, Jasmyn Pangilinan, William Andreopoulos, Richard D. Hayes, Vivian Ng, Igor V. Grigoriev, Stephen A. Jackson, Thomas D. S. Sutton, Alan D. W. Dobson, Teppo Rämä

“…Analysis of its CAZyme repertoire and a culturing experiment on three marine and one terrestrial substrates indicated that E. atlantica is a psychrotrophic generalist fungus that is able to degrade several types of marine biomass. …”
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Effect of Parental Migration Background on Childhood Nutrition, Physical Activity, and Body Mass Index by Mohsen Besharat Pour, Anna Bergström, Matteo Bottai, Inger Kull, Magnus Wickman, Niclas Håkansson, Alicja Wolk, Tahereh Moradi

“…A low level of parental education was associated with increased risk of low physical activity regardless of immigration background. Conclusions. Culturally appropriate tools to capture the diverse range of ethnic foods and other lifestyle habits are needed. …”
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Primeiras representações da identidade de gênero e da diversidade nas histórias em quadrinhos de super-heróis estadunidenses by Valéria Aparecida Bari, Rubem Borges Teixeira Ramos

“…Contudo, esses estudos se referem mais à acessibilidade, apresentação, mediação e formação do perfil leitor, a partir da produção literária já consagrada pela academia ou disseminada com sucesso pela indústria cultural. Sob a temática dos estudos de gênero, a presente pesquisa tem por objetivo estabelecer um panorama das recentes alterações na representação de personagens com diversidade de gênero, ou seja, pertencentes à comunidade LGBTQIAPN. …”
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Microbial Colonization and Antibiotic Resistance Profiles in Chronic Wounds: A Comparative Study of Hidradenitis Suppurativa and Venous Ulcers by Florica Sandru, Elena Poenaru, Smaranda Stoleru, Andreea-Maria Radu, Alexandra-Maria Roman, Corina Ionescu, Aurelian Zugravu, Jafal Mugurel Nader, Livia-Cristiana Băicoianu-Nițescu

“…<b>Methods</b>: We conducted a cross-sectional analysis that included a total of 112 individuals (24 with the diagnosis of hidradenitis suppurativa and 88 diagnosed with venous ulcer). Wound swabs were cultured to identify bacterial species, and antibiotic resistance was assessed using a standard panel of antibiotics. …”
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Assessing infection related to short-term central venous catheters in the perioperative setting by Ángel Becerra-Bolaños, Yurena Domínguez-Díaz, Héctor Trujillo-Morales, Sergio Cabrera-Doreste, Oto Padrón-Ruiz, Lucía Valencia-Sola, Nazario Ojeda-Betancor, Aurelio Rodríguez-Pérez

“…Patients with current infection, taking preoperative antibiotics, those planning to have CVC for longer than 14 days, those under 18 years old, and those refusing to participate were excluded. Patients without cultures at the moment of CVC retrieval were not included. 200 patients were included, with a mean catheter duration of 6.8 ± 3.1 days, and a total duration of 1,358 days. …”
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Salutogenic Environmental Health Model—proposing an integrative and interdisciplinary lens on the genesis of health by Jule Anna Pleyer, Laura Dominique Pesliak, Annette Konstanze Fides Malsch, Annette Konstanze Fides Malsch, Timothy McCall, Timothy McCall

“…Furthermore, the study will integrate examples of models and theories from various disciplines to illustrate how a more comprehensive and holistic explanation of health development can be provided from an interdisciplinary environmental public health perspective.MethodologyAs part of a three-step approach, a Basic Model was first developed that integrates the five environmental dimensions—Natural, Built-Material, Socio-Cultural, Psycho-Social, and Individual—into the Salutogenic Model. …”
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Five lessons from a mid-level health manager intervention to increase uptake of tuberculosis prevention therapy in Uganda: ‘it is a completely different thing to implement what you... by Jason Johnson-Peretz, Canice Christian, Cecilia Akatukwasa, Fred Atwine, Elijah Kakande, Moses R. Kamya, Diane V. Havlir, Carol S. Camlin, Gabriel Chamie

“…Sustainable application of that knowledge, or implementation know-how, must resonate with local cultural patterns. When it is neglected, root issues like unclear decision-making space and local authority to interpret policy during implementation remain unaddressed. …”
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Líneas de investigación que incluyen salud mental como prioridad de estudio en universidades peruanas by Oscar Javier Mamani-Benito, Renzo Felipe Carranza-Esteban, Tomás Caycho-Rodríguez, Ibraín Enrique Corrales-Reyes, Christian R. Mejia

“…Resultados: El 48 % de líneas de investigación considera como prioridad de estudio a los factores asociados a la depresión, violencia, conductas adictivas, psicosis y demencias; un 20 % al estado de la salud mental negativa y positiva; 4 % al desarrollo de estrategias intersectoriales con adecuación cultural, y otro 4 % a la evaluación de estrategias de prevención y promoción en salud mental con participación intersectorial. …”
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