Amyloidogenic immunoglobulin light chains disturb contractile function and calcium transients in a human cardiac spheroid model of light chain (AL) amyloidosis by Mélanie Bézard, Jean-Sébastien Vartanian-Grimaldi, Julien Henri, Denisa Calin, Amira Zaroui, Mounira Kharoubi, Thibaud Damy, Onnik Agbulut, Ekaterini Kordeli

“…LC-treated hiPSC-CM cultures and LC-containing spheroids presented structural and functional defects including: (1) decreased levels and subcellular disorganization of sarcomeric protein alpha-actinin; (2) abnormal accumulation of calcium handling SERCA2a protein; (3) impaired contractility of spheroids and altered calcium transients. …”
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Biocontrol efficacy of Bacillus licheniformis and Bacillus amyloliquefaciens against rice pathogens by Mohsin Tariq, Mehvish Zahoor, Tahira Yasmeen, Tahir Naqqash, Muhammad Abdul Rehman Rashid, Muhammad Abdullah, Abdul Rafay Rafiq, Marriam Zafar, Iqra Irfan, Ijaz Rasul

“…Based on these properties, 36 bacteria were selected and tested for biocontrol potential against rice pathogens via co-culturing antagonism assay. LE7 exhibited the maximum inhibition of 79%, while FR8, PE2, LE7, LR22 and LR28 also significantly reduced the growth of Xoo. …”
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Fossil Diatoms Reveal Natural and Anthropogenic History of Jackson Lake (Wyoming, USA) by John Dilworth, Jeffery R. Stone, Kevin M. Yeager, J. Ryan Thigpen, Michael M. McGlue

“…Jackson Lake supplies valuable cultural and provisioning ecosystem services to the Upper Snake River watershed in Wyoming and Idaho (western USA). …”
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An automated platform for simultaneous, longitudinal analysis of engineered neuromuscular tissues for applications in neurotoxin potency testing by Jacob W. Fleming, Molly C. McCloskey, Kevin Gray, David R. Nash, Vincent Leung, Christos Michas, Shawn M. Luttrell, Christopher Cavanaugh, Julie Mathieu, Shawn Mcquire, Mark Bothwell, David L. Mack, Nicholas A. Geisse, Alec S.T. Smith

“…We present a turnkey iPSC-based model of the NMJ employing channelrhodopsin-2 expression within the motor neuron (MN) population driving muscle contraction in response to blue light. MNs co-cultured with engineered skeletal muscle tissues produced twitch forces of 34.7 ± 22.7 µN in response to blue light, with a response fidelity > 92 %. …”
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Improving community-based first response to out of hospital cardiac arrest (FirstCPR): protocol for a cluster randomised controlled trial by Sandra Ware, Blake Angell, Julie Redfern, Clara Chow, Cate Ferry, Lee Taylor, Christopher Semsarian, Adrian Bauman, Janet E Bray, Simone Marschner, Michael Nelson, Saurabh Kumar, Matthew Vukasovic, Kevin Lai, Paul M Middleton, Pramesh Kovoor, Garry Jennings, Sonali Munot, Andrew Coggins, Alan Robert Denniss, Sarah Khanlari, Ian Opperman

“…The study employs a novel approach to reaching community members via social and cultural groups, and the intervention aims to address commonly cited barriers to training including lack of availability, time and costs.Methods and analysis FirstCPR is a cluster randomised trial that will be conducted across 200 community groups in urban and regional Australia. …”
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Salinity affects the lipid metabolism and intestinal microbial composition of swimming crab Portunus trituberculatus by Meimei Liu, Tong Li, Hao Zhang, Zi Wang, Xugan Wu, Jie He, Zhiguo Dong

“…This study investigated the hepatopancreatic index, hepatopancreatic lipid metabolism, physiological and biochemical indicators and gut microbiota of P. trituberculatus cultured at salinity 28 ‰, 25 ‰, 22 ‰ and 19 ‰ for 8 weeks. …”
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Functional Skin Grafts: Where Biomaterials Meet Stem Cells by Amtoj Kaur, Swati Midha, Shibashish Giri, Sujata Mohanty

“…The current focus of skin tissue engineers is primarily to develop a 3D construct that maintains the functionality of cultured cells in a guided manner over a period of time. …”
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Conflicts in the tourist city from the perspective of local social movements by Yolanda Romero-Padilla, Alfonso Cerezo-Medina, Enrique Navarro-Jurado, José María Romero-Martínez, Antonio Guevara-Plaza

“…Los resultados evidencian que en las ciudades con turismo clásico de litoral ya existía la vivienda de alquiler desde sus orígenes como destino y ahora se ha visibilizado porque las plataformas digitales lo han puesto en el mercado global; en ciudades con turismo urbano-cultural las viviendas son una nueva forma de alojamiento que genera nuevos conflictos; por último, los resultados contrastan las características del modelo crecentista en la ciudad turística frente a las reivindicaciones de los movimientos sociales que cuya base es la compatibilidad del turismo con la preservación y mejora de la calidad de la vida cotidiana. …”
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RELATIONSHIP BETWEEN NATIVE INTELLIGENCE (NI) AND ARTIFICIAL INTELLIGENCE (AI): AN OVERVIEW by Bawallah Musa Adesola, Odole, Kehinde, Ilugbo, Stephen Olubusol, DareC, Ojo Omonijo, Owele Boye Agatha, Hadiza Mali Bukar

“…Western Ideologists tend to attribute Native Intelligence (NI) to Africans / third-word concepts, indistinctly fixed to value and belief that aided social cultural, religious, political economic, spiritual evolution, and ancient societies, However, the Emergence of Artificial intelligence (AI) being the capacity of a computer system or machine to mimic human cognitive such as learning and solving problems, through a computer system that uses mathematics and logic, to simulate the reasoning ability of man, through the process of learning from new information, and transforming such information into actions, by making decisions that has increasingly enhanced the quality of human life. …”
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The Role of Motivation to Promote Personal and Social Responsibility Through PE and Sport: A Comparative Exploratory Investigation of Prospective PE Teachers and Sports Leaders in... by Istvan Soos, Ian Whyte, Diana Szekeres, Karoly Ozsvath, Attila Szabo, Jonathan Ling, Jose Francisco Jiménez-Parra, Alfonso Valero-Valenzuela

“…Method: A cross-sectional, cross-cultural comparative quantitative study was conducted with university students (n = 368) from Spain (n = 109 males, 67%) and Hungary (n = 108 males, 53%) using a demographic questionnaire and six validated questionnaires). …”
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Photoaging smartphone app promoting poster campaign to reduce smoking prevalence in secondary schools: the Smokerface Randomized Trial: design and baseline characteristics by Michael Kreuter, Fabian N Fries, Titus J Brinker, Ute Mons, David A Groneberg, Anil Batra, Werner Seeger, Felix Herth, Julia Holzapfel, Tanja G Baudson, Katharina Sies, Lena Jakob, Hannah Maria Baumert, Marlene Heckl, Ana Cirac, Janina L Suhre, Verena Mathes, Hannah Spielmann, Nancy Rigotti, Tobias Raupach, Henning Gall, Claudia Bauer, Pat Marek, Chase H Harrison, Lava Taha, Andreas Owczarek, Felix J Hofmann, Roger Thomas

“…Longitudinal changes in smoking-related attitudes, the number of new smokers and quitters and the change in the number of never-smokers will be compared between the two groups as secondary outcomes.Ethics and dissemination Ethical approval was obtained from the ethics committee of the University of Gießen and the ministries of cultural affairs, both in Germany. Results will be disseminated at conferences, in peer-reviewed journals, on our websites and throughout the multinational Education Against Tobacco network.Trial registration number NCT02544360, Pre-results.…”
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Oral behaviors in Chinese temporomandibular disorder patients: insights from exploratory and confirmatory factor analyses by Tiqian Liu, Tiqian Liu, Adrian Ujin Yap, Adrian Ujin Yap, Adrian Ujin Yap, Yanyu Sun, Yanyu Sun, Yunhao Zheng, Yunhao Zheng, Tianqi Wang, Tianqi Wang, Shiya Zeng, Shiya Zeng, Zhen Liu, Xin Xiong, Xin Xiong

“…Developing and validating an East–West short-form version of the OBC should be prioritized, given the variations in oral behaviors across countries and cultures.…”
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Protection of liver sinusoidal endothelial cells using different preservation solutions by Julia H E Houtzager, Anne-Marieke van Stalborch, Charlotte Hofstee, Thomas M van Gulik, Jaap D van Buul

“…Human umbilical vein endothelial cells (HUVECs) and LSECs were incubated in either University of Wisconsin machine perfusion solution (UW-MPS), histidine-tryptophan-ketoglutarate, or endothelial cell growth medium 2 (EGM2) (as a gold standard for cell culturing). Endothelial integrity was assessed by measurement of cellular morphology and expression of membrane proteins: PECAM-1, ICAM-1 and Fc-gamma receptor CD32b (FcΥRCD32b). …”
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Genome-wide identification and expression analysis of ZF-HD family in sunflower (Helianthus annuus L.) under drought and salt stresses by Kangping Zhang, Ruqian Yan, Xiaoqing Feng, Jingjing Zhang, Yuanyuan Jiang, Lin Li, Jianzhong Guo, Xianping Zhang

“…Abstract Background Sunflower (Helianthus annuus L.) is widely cultured in globally tropical and subtropical regions, with better resistance to various abiotic stresses as a model crop. …”
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Smoking behavior among Asian Americans during the initial phase of the COVID-19 pandemic: The influence of pandemic stressors and depression by Paula Lozano, Aven Peters, Alia Southworth, Yicklun Mo, Helen Lam, Fornessa T. Randal, Karen E. Kim

“…Conclusions The findings of the study highlight the need for culturally tailored smoking cessation interventions for Asian American communities that address pandemic-related stressors such as discrimination that may trigger cigarette use.…”
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MUC2 expression modulates immune infiltration in colorectal cancer by Christophe M. Raynaud, Ayesha Jabeen, Eiman I. Ahmed, Eiman I. Ahmed, Satanay Hubrack, Apryl Sanchez, Shimaa Sherif, Ahmad A. Al-Shaibi, Bernice Lo, Bernice Lo, Jessica Roelands, Davide Bedognetti, Davide Bedognetti, Wouter Hendrickx, Wouter Hendrickx

“…By employing CRISPR-mediated MUC2 knockout, we investigated the influence of MUC2 on tumor immune infiltration and its interplay with T cells and NK cells enriched peripheral blood mononuclear cells (PBMCs) in 3D spheroid cultures.ResultsWhile MUC2 was more abundant in LS-174T cell line compared to HT-29, its knockout resulted in increased immune infiltration solely in the HT-29 cell line, but not in the LS-174T cell line. …”
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Identification of Health Needs in Ukrainian Refugees Seen in a Primary Care Facility in Tenerife, Spain by Willian-Jesús Martín-Dorta, Cristo-Manuel Marrero-González, Eva-Lourdes Díaz-Hernández, Pedro-Ruymán Brito-Brito, Domingo-Ángel Fernández-Gutiérrez, Oxana-Migalievna Rebryk-De Colichón, Ana-Isabel Martín-García, Estrella Pavés-Lorenzo, María-Candelaria Rodríguez-Santos, Juan-Francisco García-Cabrera, Janet Núnez-Marrero, Alfonso-Miguel García-Hernández

“…The typical profile of a Ukrainian refugee in the Canary Islands is female (79.7%), relatively young, with a high socio-cultural background, generally in good health, travelling alone or with her minor children. …”
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‘If one doesn't happen, the other will’: forensic mental health service patients’ experiences of co-occurring self-harm and aggression by Matina Shafti, Peter Taylor, Andrew Forrester, Louise Robinson, Sandeep Mathews, Daniel Pratt

“…Conclusions Findings provide recommendations that can help address dual harm within forensic settings, including (a) transdiagnostic, individualised approaches that consider the duality of self-harm and aggression; and (b) cultural and organisational focus on recovery-centred practice. …”
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Increased Duodenal Iron Absorption through Upregulation of Ferroportin 1 due to the Decrement in Serum Hepcidin in Patients with Chronic Hepatitis C by Masanori Sato, Koji Miyanishi, Shingo Tanaka, Akira Sakurada, Hiroki Sakamoto, Yutaka Kawano, Kohichi Takada, Masayoshi Kobune, Junji Kato

“…The FPN mRNA levels and protein levels increased significantly in Caco-2/TC7 cell monolayers cultured in transwells with hepcidin. Lower serum hepcidin-25 levels might upregulate not only FPN protein expression but also mRNA expression in the duodenum and cause iron accumulation in patients with CHC.…”
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High-Affinity Anti-VISTA Antibody Protects against Sepsis by Inhibition of T Lymphocyte Apoptosis and Suppression of the Inflammatory Response by Tianzhu Tao, Lulong Bo, Teng Li, Longbao Shi, Hui Zhang, Bo Ye, Yuhai Xu, Qingqing Ma, Xiaoming Deng, Guorong Zhang

“…Engagement of VISTA receptor with MH5A mitigated CD3+ T cell apoptosis cultured from CLP mice and suppressed LPS-induced cytokine production by macrophage in vitro. …”
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