Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine by Seyed Muhammad Hamid Malekpour, Moj Khaleghi, Alireza Akhtarpour

“…Twenty strains were isolated from the soil of Langar, Kerman, Iran, with characteristics of actinobacteria (gram-positive filamentous or rod-shaped bacteria and powdery consistency colonies that stick firmly to agar surface). All strains were cultured in a medium with L-tyrosine to assess tyrosinase production. …”
Get full text

Carrageenan-Induced Colonic Inflammation Is Reduced in Bcl10 Null Mice and Increased in IL-10-Deficient Mice by Sumit Bhattacharyya, Liquan Xue, Suzanne Devkota, Eugene Chang, Stephan Morris, Joanne K. Tobacman

“…IL-10-deficient mice exposed to carrageenan in a germ-free environment showed an increase in activation of the canonical pathway of NF-κB (RelA) activation, but without increase in RelB or phospho-Bcl10, and exogenous IL-10 inhibited only the canonical pathway of NF-κB activation in cultured colonic cells. These findings demonstrate a Bcl10 requirement for maximum development of carrageenan-induced inflammation and lack of complete suppression by IL-10 of carrageenan-induced inflammation.…”
Get full text

Understanding co-production of injury research in Australian Aboriginal and Torres Strait Islander communities: a comprehensive scoping review by Genevieve Westacott, Victoria McCreanor, Susanna Cramb, Silvia Manzanero, Kim Vuong, Michelle Allen, Shannon Dias, Geoffrey Binge, Arpita Das

“…This helped the collection of culturally safe data and appropriate interpretation of results. …”
Get full text

Functional assessment of cancer therapy – head & neck (FACT-HN) translation and validation in Serbian by Masa Petrovic, Bojana Salovic, Ana Tomic, Tanja Jesic-Petrovic, Aleksandar Matejic, Ivana Radovic, Silvana Babic, Aleksa Korugic, Drago Jelovac, Nikola Mikovic, Sasa Jakovljevic, Svetislav Pelemis, Milovan Dimitrijevic, Jovica Milovanovic, Ana Jotic, Aleksandar Trivic, Ana Dimitrijevic, Sulin Bulatovic, Zoran Dudvarski, Ivan Soldatovic, Jelena Ilic-Zivojinovic

“…The research focuses on determining the translated questionnaire’s reliability, validity, and cultural relevance. A total of 106 Serbian-speaking head and neck cancer patients completed the translated FACT-HN, along with other validated instruments, including the EORTC QLQ-C30, EORTC QLQ-HN43, CES-D, and GAD-7. …”
Get full text

Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38/MAPK Pathway by Xiuxia Wang, Yinmin Wang, Xianyu Zhou, Fei Liu

“…The transcript abundance of TGF-β1, as well as that of insulin-like growth factor binding protein 3 (IGF-BP3), was evaluated using qRT-PCR on Jurkat cells cultured in ADSC-CM for 24 hours. The proliferation of the Jurkat cells was assessed using cell cycle assay. …”
Get full text

HMGB1 released from pyroptotic vascular endothelial cells promotes immune disorders in exertional heatstroke by Chaoping Yu, Yang Huang, Jiangang Xie, Chujun Duan, Shanshou Liu, Wei Zhao, Yutong Wang, Ran Zhuang, Junjie Li, Wen Yin

“…Human umbilical vein endothelial cells (HUVECs) and THP-1 cells were co-cultured to study the effects of HMGB1 on macrophages. …”
Get full text

Muscarinic cannabinoid suppression of excitation, a novel form of coincidence detection by Michaela Dvorakova, Ken Mackie, Alex Straiker

“…These receptors are implicated in several forms of synaptic plasticity – depolarization-induced suppression of excitation (DSE), metabotropic suppression of excitation (MSE), long term depression (LTD) and activation-dependent desensitization. Cultured autaptic hippocampal neurons express all of these, illustrating the rich functional and temporal heterogeneity of CB1 at a single set of synapses. …”
Get full text

Bile acids in the lower airways is associated with airway microbiota changes in chronic obstructive pulmonary disease: an observational study by Peter A B Wark, Siobhain Mulrennan, Stephen Stick, Yuben P Moodley, Kristy S Nichol, Jose A Caparros-Martin, Montserrat Saladié, S Patricia Agudelo-Romero, F Jerry Reen, Fergal O’Gara

“…Likewise, the odds of recovering bacterial cultures from BAL were higher if BAs were also detected. …”
Get full text

Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells by Antonella Bordin, Francesca Pagano, Eleonora Scaccia, Matteo Saccucci, Iole Vozza, Noemi Incerti, Antonella Polimeni, Elena Cavarretta, Isotta Chimenti, Elena De Falco

“…Dental pulp stem cells (DPSCs), derived from the premolar tooth, were conditioned for 21 days with oral plaque samples and tested for their clonogenic ability. Cultures were also induced to differentiate towards the osteogenic lineage, and ALP and osteocalcin gene expression levels were evaluated by real-time qPCR. …”
Get full text

Rothia mucilaginosa Meningitis in a Child with Myelodysplastic Syndromes by Fumihiro Ochi, Ryota Nakamura, Reiji Miyawaki, Kyoko Moritani, Shinobu Murakami, Hisamichi Tauchi

“…We retrospectively reviewed the antimicrobial susceptibility of all R. mucilaginosa isolates (n = 5) from blood or cerebrospinal fluid cultures at our hospital. The MIC of VCM was <0.5 μg/mL for all strains, whereas the MIC of DAP was ≥2 μg/mL for all strains. …”
Get full text

TRAIL and Ceruloplasmin Inverse Correlation as a Representative Crosstalk between Inflammation and Oxidative Stress by Veronica Tisato, Stefania Gallo, Elisabetta Melloni, Claudio Celeghini, Angelina Passaro, Giorgio Zauli, Paola Secchiero, Carlo Bergamini, Alessandro Trentini, Gloria Bonaccorsi, Giuseppe Valacchi, Giovanni Zuliani, Carlo Cervellati

“…After stratification for high-sensitivity C-reactive protein levels, the inverse association between TRAIL and ceruloplasmin levels remained strong and significant (r=−0.508, p<0.001, R2=0.260) only in the presence of inflammation, confirming the role of inflammation as emerged in in vitro experiments where recombinant TRAIL decreased ceruloplasmin expression levels in TNF-treated PBMC cultures. Conclusion. The results indicated that in an inflammatory milieu, TRAIL downregulates ceruloplasmin expression, highlighting a signaling axis involving TRAIL and ceruloplasmin that are linked via inflammation and providing important insights with potential clinical implications.…”
Get full text

Predictors of schoolteachers' intention to report suspected child abuse and neglect cases in Oman: A national study by Zeinab Al Azri, Wafa A.L. Jabri, Iman Al Hashmi, Asia Alnaamani, Omar Al Zaabi

“…These results provide stakeholders with directions for designing culturally sensitive interventions to enhance the reporting of suspected child abuse cases in Oman.…”
Get full text

Analysis of the anthropogenic effect on the Silencio River in Salvador Escalante, Michoacán, México by Mario Alberto Pérez-Méndez, Guadalupe Selene Fraga-Cruz, Gustavo Álvarez-Gómez, Fabricio Nápoles-Rivera, Gladys Jiménez-García, Rafael Maya-Yescas

“…This aims to evaluate the capacity of the WWTP for improve the water quality. Microbiologic cultures were used for a qualitative study of the microorganisms contained in the active sludges; it was found that Enterobacterium does not grow in presence of heavy metals. …”
Get full text

The influence of adding B. subtilis bacteria on the mechanical and chemical properties of cement mortar by Osama Ahmed Ibrahim, Ahmed Ibrahim Hassanin Mohamed, Wael Ibrahim, Raghda Osama Abd-Al Ftah, Shimaa R. Hamed, Sherif Fakhry M. Abd-Elnaby

“…This was done by using soil samples from Sharkia, Egypt, to isolate 48 bacterial strains, after which they were cultured using the Johnson method and various media. …”
Get full text

“I think we're on a cusp of some change:” coping and support for mental wellness among Black American women by Terika McCall, Terika McCall, Terika McCall, Meagan Foster, Holly Tomlin, Holly Tomlin, Bolatito Adepoju, Mckenzie Bolton-Johnson, Chyrell D. Bellamy

“…Intentional inclusion of Black women in mental health research and evolving treatment paradigms is important to eliminate inequities in access to culturally relevant mental health care.…”
Get full text

Re-conceptualizing trafficking-in-persons victimization using latent class analysis: Results from a community study in Cape Town, South Africa by Annah K. Bender, Erica L. Koegler, Edna G. Rich, Nicolette V. Roman, Rumi Kato Price

“…The difficulty of reaching a clandestine population is complicated by the lack of standardized definitions and culturally responsive assessments to identify victims and route them to appropriate care. …”
Get full text

The mechanism of endoplasmic reticulum (ER) stress in cell apoptosis and ROS (reactive oxygen species) of CNE2 cell line induced by single wall carbon nanohorn (SWCNH) by Xianbao Cao, Shunmin Gong, Shujin Chen, Shuang Hu, Tianshu Li, Yanfei Guan, Chun Feng, Jinqian Zhang

“…Therefore, the biological roles of raw single wall carbon nanohorn (SWCNH) on endoplasmic reticulum (ER) stress in the apoptosis of CNE2 were explored.MethodsTherefore, ERS of CNE2 cells was induced by SWCNH, and 4-phenylbutyrate (4-PBA) was selected as a inhibitor of ERS. CNE2 cells were co-cultured with SWCNH, 4-PBA and SWCNH+4-PBA, respectively. …”
Get full text

A human skin-on-a-chip platform for microneedling-driven skin cancer treatment by Natan R. Barros, Raehui Kang, Jinjoo Kim, Menekse Ermis, Han-Jun Kim, Mehmet R. Dokmeci, Junmin Lee

“…Skin-on-a-chip models provide physiologically relevant platforms for studying diseases and drug evaluation, replicating the native skin structures and functions more accurately than traditional 2D or simple 3D cultures. However, challenges remain in creating models suitable for microneedling applications and monitoring, as well as developing skin cancer models for analysis and targeted therapy. …”
Get full text

Fungal Keratitis Caused by Drechslera spp. Treated with Voriconazole: A Case Report by Margarita I. Echavez, Archimedes Lee D. Agahan, Noel S. Carino

“…Corneal scraping of the ulcer showed no organisms on Gram and Giemsa stain. Cultures were positive for Drechslera spp. and patient was started on Natamycin drops every 15 minutes, Atropine drops 3× a day, and Levofloxacin was continued every 4 hours. …”
Get full text

Challenges Faced by Prehospital Emergency Physicians Providing Emergency Care to Patients with Advanced Incurable Diseases by Anne Kamphausen, Hanna Roese, Karin Oechsle, Malte Issleib, Christian Zöllner, Carsten Bokemeyer, Anneke Ullrich

“…We identified nine categories of challenges: structural conditions of prehospital emergency care, medical documentation and orders, finding optimal patient-centered therapy, uncertainty about legal consequences, challenges at the individual (EP) level, challenges at the emergency team level, family caregiver’s emotions, coping and understanding of patient’s illness, patient’s wishes, coping and understanding of patient’s illness, and social, cultural, and religious background of patients and families. …”
Get full text