Artemisia capillaris with two novel active compounds, Kawarayomogin I and II, inhibits HYBID (KIAA1199) expression as well as hyaluronic acid degradation by Kazal Boron Biswas, Yuka Kawai, Satoshi Nakagawa, Kyoko Kanai, Hiroyuki Kojima, Teruaki Masutani, Masayoshi Oyama, Arunasiri Iddamalgoda, Kotaro Sakamoto

“…The molecular size distribution of HA was evaluated by incubating fluorescein isothiocyanate (FITC)-labeled large HA (1200–1600 kDa) in NHDF for certain time followed by measuring different sizes of FITC-labeled HA in the cultured medium by HPLC. Among 380 plant extracts, we found that Artemisia capillaris flower extract (ACFE) was the most effective agent in both suppressing HYBID expression as well as protecting large HA from degradation. …”
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Optimizing winged-bean meal through oven and autoclave heating as viable alternative for plant-based aquafeed by Shobrina Silmi Qori Tartila, Tholibah Mujtahidah, Muh. Azril, Anjali Apta Pramudita, Lusi Septiani, Bagas Putra Satria, Ignatius Abel Cristanto, Muhammad Abizar Sakti Oktavian, Sultan Jibran Risqulloh, Santika Dewi Astuti

“…Data were also compared to the nutrient requirements of several cultured freshwater fish in Indonesia. Heating treatments improved in vitro trypsin activity (1300 units/O and 1135 units/A vs. 835 units) but reduced protein (29.05±1.96%/A and 31.73±1.52%/O vs. 35.17±0.38%) and moisture contents. …”
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Transcriptional Regulation of Δ6-Desaturase by Peroxisome Proliferative-Activated Receptor δ Agonist in Human Pancreatic Cancer Cells: Role of MEK/ERK1/2 Pathway by Maryam Darabi, Shima Byagowi, Shabnam Fayezi, Masoud Darabi, Shahab Mirshahvaladi, Mehdi Sahmani

“…The aim of the present study was to investigate the effect of peroxisome proliferative-activated receptor δ (PPARδ) agonist and MEK/ERK1/2-dependent pathway on the expression of Δ6D in human pancreatic carcinoma cell line PANC-1. PANC-1 cells cultured in RPMI-1640 were exposed to the commonly used ERK1/2 pathway inhibitor PD98059 and PPARδ agonist GW0742. …”
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Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…Human DPSCs were isolated and cultured in osteogenic induction medium with or without RA or BMS 493, an inverse agonist of the pan-retinoic acid receptors (pan-RARs). …”
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Numerical Modeling and Stability Analysis of Surrounding Rock of Yuanjue Cave by Zhigang Meng, Fangzheng Fan, Xuebin Cui, Shu Tao, Yi Cao

“…Yuanjue Cave, located in Big Buddha Bay, Mount Baoding, Dazu Rock Carvings Area, has extremely high historical, artistic, and religious value and is an important grotto cultural relic in China. Due to the cutting action of the fissures and weak interlayers, the South Cliff of Big Buddha Bay where Yuanjue Cave is located showed signs of instability. …”
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Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation by Najmeh Anbiaee, Mohammad Taghi Bahreyni Toossi, Hosein Azimian, Zoha Sahebnasagh, Sepideh Abdollahi Dehkordi, Majid Kianmehr, samaneh soudmand salarabadi, Maryam Najafi Amiri

“…The protective effects of the green tea polyphenol on Bcl-2 and Bax genes expression levels were evaluated in cultured lymphocytes obtained from treated cells (24 h and 1 h before and 1 h after exposure to ionizing radiation) and non-treated cells (Receiving X-radiation and non-receiving green tea).Results: The results of this investigation demonstrated Bax/Bcl-2 ratio, as an index of the radiation sensitivity, was significantly decreased in groups which had been received dose of 2 Gy in treated cells groups of 1 h before and 1 h after X-radiation (p<0.05).Conclusion: The results indicated different effects of green tea on low and high doses of X-radiation. …”
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Comparison evaluation of bacterial DNA extraction methods for improved molecular diagnostic accuracy of sepsis-causing pathogens in clinical whole blood samples by Byungjoon Na, Junghun Park, Sojin Park, Eunseon Park, Jimin Jang, Yu-Hee Kim, Jinyeop Lee, Hae-Sun Chung

“…Current diagnostic methods such as blood cultures are time-consuming, whereas molecular diagnostic techniques represent a promising alternative for faster pathogen detection. …”
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The Efficacy of Single-Application NPWTi-d for the Salvage of Infected Breast Prostheses: A Multi-Center Study by Jason C. Llaneras, MD, Robert Craig Clark, MD, Lauren Antognoli, MD, Emily Finkelstein, MD, Luci Hulsman, BS, Luther Holton, MD, Devinder Singh, MD, R. Jason VonderHaar, MD, Risal Djohan, MD, Aladdin H. Hassanein, MD, Chris M. Reid, MD

“…Methicillin-resistant organisms were cultured in 15% of cases. NPWTi-d was applied for an average of 61 hours with dwell times of 18 minutes every 3 hours, using institution-specific instillates. …”
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A stretchable human lung‐on‐chip model of alveolar inflammation for evaluating anti‐inflammatory drug response by Clémentine Richter, Lorenz Latta, Daria Harig, Patrick Carius, Janick D. Stucki, Nina Hobi, Andreas Hugi, Paul Schumacher, Tobias Krebs, Alexander Gamrekeli, Felix Stöckle, Klaus Urbschat, Galia Montalvo, Franziska Lautenschläger, Brigitta Loretz, Alberto Hidalgo, Nicole Schneider‐Daum, Claus‐Michael Lehr

“…The model is based on the human alveolar epithelial cell line Arlo co‐cultured with macrophages differentiated from the THP‐1 cell line, creating a physiological biological microenvironment. …”
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Lettuce seedlings quality: The effects of white and blue light exposure by Luković Kristina, Adžić Slađan, Zečević Veselinka, Đurić Nenad, Perišić Vladimir, Gavrilović Bojana, Rakonjac Aleksandra

“…In the phase of three leaves, plants were placed in plant growth chambers under multispectral white (W) LEDs and monochromatic blue (B) LEDs (three containers per LED and genotype), while three containers were kept in the greenhouse and served as control. The plants were cultured in plant growth chambers for 25 days under a 9h/15h and 10h/14h (light/dark) photoperiod, to simulate outdoor conditions. …”
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Huntington’s disease modeling on HEK293 cell line by D. V. Sharipova, T. B. Malankhanova, A. A. Malakhova

“…Therefore, one of the important tasks is generation of an in vitro model system of Huntington’s disease based on human cell cultures. The new genome editing approaches, such as CRISPR/Cas9, allow us to generate isogenic cell lines that can be useful for drug screening and studying mechanisms of molecular and cellular events triggered by certain mutation on an equal genetic background. …”
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The philosophical foundations of digital twinning by David J Wagg, Christopher Burr, Jason Shepherd, Zack Xuereb Conti, Mark Enzer, Steven Niederer

“…As a result, members of the newly forming digital twin community are often talking at cross-purposes, based on different starting points, assumptions, and cultural practices. These differences are due to the philosophical world-view adopted within specific communities. …”
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Lyophilized and Oven-Dried <i>Manilkara zapota</i> Extracts: Characterization and <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Analyses by María Fernanda Rivas-Gastélum, Patricia Ariadna Galindo-Castillo, Juan Esparza-Sánchez, Miriam Irene Jiménez-Pérez, Yocanxóchitl Perfecto-Avalos, Luis Eduardo Garcia-Amezquita, Diego E. Navarro-López, Edgar R. López-Mena, Eugenio Sánchez-Arreola, Juan Pablo Tamayo-Martínez, Humberto L. Mendoza-Figueroa, María Magdalena Crosby-Galván, Elsa Margarita Crosby-Galván, Jorge L. Mejía-Méndez, Angélica Lizeth Sánchez-López

“…<i>In silico</i> evaluation unveiled that polar compounds in <i>M. zapota</i> fruits possess a high binding affinity towards the DNA gyrase B of the cultured strains. This study expands the scientific evidence regarding the influence of distinct extraction methods on the nutritional and nutraceutical content of native fruits and the importance of considering additional approaches to enhance their bioactivities.…”
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Migraine in men by Mira P. Fitzek, Deirdre M. Boucherie, Tessa de Vries, Cleo Handtmann, Haniyeh Fathi, Bianca Raffaelli, Antoinette MaassenVanDenBrink

“…The reasons for these sex disparities are complex, involving biological, psychosocial, and cultural factors, such as brain structural differences, differences in functional responses to painful stimuli, hormonal effects, and behavioral influences like adherence to masculine norms and stigma. …”
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Building resilience against cholera: lessons from the implementation of integrated community strategy for cholera control in Zambia by Nyuma Mbewe, Fred Kapaya, Moses Mwale, Peter Jay Chipimo, Precious Kalubula, Ladislas Hibusu, Stella Mumba Chomba Mulima, Kafusha Kapema, Kelvin Mwangilwa

“…The strategy’s emphasis on data-driven decision-making and adaptation to local socio-cultural dynamics was crucial for its effectiveness. …”
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Decoding Urinary Tract Infection Trends: A 5-Year Snapshot from Central Portugal by Francisco Rodrigues, Patrícia Coelho, Sónia Mateus, Armando Caseiro, Hatem Eideh, Teresa Gonçalves, Miguel Castelo Branco

“…Methods: A total of 6161 positive urine cultures collected over five years were examined, with particular emphasis on 2019 due to a peak in infection rates. …”
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A multicenter, single‐group, open feasibility study of a new individual cognitive behavioral therapy program for adult Japanese patients with attention‐deficit/hyperactivity disord... by Naoki Takamatsu, Misuzu Nakashima, Kei Matsuura, Ikue Umemoto, Masaya Ito, Junichiro Kanazawa, Hirofumi Tomiyama, Masaki Kondo, Hisateru Tachimori, Tomohiro Nakao, Masaru Horikoshi, Hironori Kuga

“…Japan lacks unified CBT programs and adult ADHD guidelines, making it difficult to implement international individual CBT recommendations due to cultural differences. This study will assess the feasibility of a novel CBT program for Japanese adult ADHD patients. …”
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Hypoxia lowers cell carbon and nitrogen content and accelerates sinking of a marine diatom Thalassiosira pseudonana by Bokun Chen, Bokun Chen, Hui Song, Hui Song, Ge Xu, Hongli Ji, Hongli Ji, Xue Yang, Xue Yang, Gang Li

“…To uncover the underlying mechanisms, the marine model diatom Thalassiosira pseudonana was cultured under three dissolved O2 levels (8.0 mg L-1, ambient O2; 4.0 mg L-1, low O2; and 1.3 mg L-1, hypoxia) to compare its growth, cell composition, and physiology between the light and dark periods. …”
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NF-κB Inhibition after Cecal Ligation and Puncture Reduces Sepsis-Associated Lung Injury without Altering Bacterial Host Defense by Hui Li, Wei Han, Vasilly Polosukhin, Fiona E. Yull, Brahm H. Segal, Can-Mao Xie, Timothy S. Blackwell

“…Treatment with the NF-κB inhibitor did not affect the ability of cultured macrophages to phagocytose bacteria and did not alter bacterial colony counts in blood, lung tissue, or peritoneal fluid at 24 hours after CLP. …”
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Electrophysiology-based screening identifies neuronal HtrA serine peptidase 2 (HTRA2) as a synaptic plasticity regulator participating in tauopathy by Naizhen Zheng, Kun Li, Jing Cao, Zijie Wang, Liang Zhang, Zihao Zhao, Jiawei He, Yong Wang, Xiang Zhu, Yiqing Chen, Jian Meng, Dongdong Zhao, Mengxi Niu, Hong Luo, Xian Zhang, Hao Sun, Yun-wu Zhang

“…HTRA2 downregulation impaired synapses and reduced ATP production in cultured primary neurons. Furthermore, adeno-associated virus (AAV)-mediated HTRA2 downregulation in the hippocampus impaired synaptic plasticity and cognitive function in C57BL/6 mice. …”
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