Targeted sequencing analysis pipeline for species identification of human pathogenic fungi using long-read nanopore sequencing by Nattapong Langsiri, Navaporn Worasilchai, Laszlo Irinyi, Piroon Jenjaroenpun, Thidathip Wongsurawat, Janet Jennifer Luangsa-ard, Wieland Meyer, Ariya Chindamporn

“…The species used in this study included Candida albicans (n = 2), Candida tropicalis (n = 1), Nakaseomyces glabratus (formerly Candida glabrata) (n = 1), Trichosporon asahii (n = 2), Pichia kudriavzevii (formerly Candida krusei) (n = 1), and Cryptococcus neoformans (n = 1). Comparing various methods to generate the consensus sequence for fungal species identification, the results from this study indicate that read clustering using a modified version of the NanoCLUST pipeline is more sensitive than Canu or VSEARCH, as it classified species accurately with a lower abundance cluster of reads (3% abundance compared to 10% with VSEARCH). …”
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Gcn2 rescues reprogramming in the absence of Hog1/p38 signaling in C. neoformans during thermal stress by David Goich, Amanda L. M. Bloom, Sean R. Duffy, Maritza N. Ventura, John C. Panepinto

“…ABSTRACT The fungus Cryptococcus neoformans is an opportunistic pathogen of humans that reprograms its translatome to facilitate adaptation and virulence within the host. …”
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Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells by Michèle Stella Majoumouo, Marius Belmondo Tincho, Rufin Marie Kouipou Toghueo, Thureyah Morris, Donavon Charles Hiss, Fabrice Fekam Boyom, Chitra Mandal

“…Endophyte extracts N2, N7, N8, N97, N169, and N233 were obtained from Trichoderma sp, Phoma sp, Phomopsis phyllanticola, Fusarium oxyporum, Collectotrichum sp, and Cryptococcus flavescens, respectively. The N97 extract was most active with a 50% inhibitory concentration (IC50) of 33.35 µg/ml. …”
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Antimicrobial Resistance Profile and Biofilm Production of Microorganisms Isolated from Oropharynx of Rupornis magnirostris (Gmelin, 1788) and Caracara plancus (Miller, 1777) by Fernanda Alda da Silva, Sandrelli Meridiana de Fátima Ramos dos Santos Medeiros, Sérgio Dias da Costa-Junior, Ana Emília Medeiros Roberto, Sarah Brandão Palácio, Reginaldo Gonçalves de Lima-Neto, Rejane Pereira Neves, Carolina Peixoto Magalhães, José Eduardo Garcia, Isabella Macário Ferro Cavalcanti

“…Regards the fungal analysis, Candida spp., Cryptococcus spp., Rhodotorula mucilaginosa, R. glutinis, and Trichosporon coremiiforme were identified. …”
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Primary Cutaneous Cryptococcosis Treated with Debridement and Fluconazole Monotherapy in an Immunosuppressed Patient: A Case Report and Review of the Literature by Jennifer Wang, Luther Bartelt, Deborah Yu, Anjali Joshi, Bradley Weinbaum, Tiffany Pierson, Michael Patrizio, Cirle A. Warren, Molly A. Hughes, Gerald Donowitz

“…Cryptococcus neoformans is an opportunistic yeast present in the environment. …”
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A Case of Recurrent Cryptococcal Meningoencephalitis in an Immunocompetent Female by Negin Niknam, Negar Niknam, Kola Dushaj, Erfidia Restrepo

“…Cryptococcus neoformans is commonly associated with meningoencephalitis in immunocompromised patients and occasionally in apparently healthy individuals. …”
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