Serious issues with cryo-EM structures of human prothrombinase by James A. Huntington, Alexandre Faille, Fatma Isik Ustok

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Scaffold-enabled high-resolution cryo-EM structure determination of RNA by Daniel B. Haack, Boris Rudolfs, Shouhong Jin, Alexandra Khitun, Kevin M. Weeks, Navtej Toor

“…Abstract Cryo-EM structure determination of protein-free RNAs has remained difficult with most attempts yielding low to moderate resolution and lacking nucleotide-level detail. …”
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Parallelizing the cryo‐EM structure determination in THUNDER using GPU cluster by Zhao Wang, Huabin Ruan, Guangwen Yang, Xueming Li

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Cryo-EM structure and evolutionary history of the conjugation surface exclusion protein TraT by Chloe Seddon, Sophia David, Joshua L. C. Wong, Naito Ishimoto, Shan He, Jonathan Bradshaw, Wen Wen Low, Gad Frankel, Konstantinos Beis

“…Here, we show that TraT from the pKpQIL and F plasmids (TraTpKpQIL and TraTF) exhibits plasmid surface exclusion specificity. The cryo-EM structures of TraTpKpQIL and TraTF reveal that they oligomerise into decameric champagne bottle cork-like structures, which are anchored to the outer membrane via a diacylglycerol and palmitic acid modified α-helical barrel domain. …”
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Protein identification using Cryo-EM and artificial intelligence guides improved sample purification by Kenneth D. Carr, Dane Evan D. Zambrano, Connor Weidle, Alex Goodson, Helen E. Eisenach, Harley Pyles, Alexis Courbet, Neil P. King, Andrew J. Borst

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Non-uniform Fourier transform based image classification in single-particle Cryo-EM by ZiJian Bai, Jian Huang

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AI-based quality assessment methods for protein structure models from cryo-EM by Han Zhu, Genki Terashi, Farhanaz Farheen, Tsukasa Nakamura, Daisuke Kihara

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Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure by Ranhao Zhang, Yuan Shen, Xueming Li

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High-resolution Cryo-EM Structure Determination of a-Synuclein—A Prototypical Amyloid Fibril by Juan Sanchez, Joshua Pierson, Collin Borcik, Chad Rienstra, Elizabeth Wright

“…We provide a detailed step-by-step protocol for high-resolution cryo-EM structure determination of a-syn fibrils using RELION and a series of specialized helical reconstruction tools that can be run within RELION. …”
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Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering by William S. Grubbe, Bixia Zhang, Aileen Kauffman, Fabian Byléhn, Kasia Padoł, Hae-Gwang Jung, Seung Bum Park, Jessica M. Priest, Engin Özkan, Juan J. de Pablo, T. Jake Liang, Minglei Zhao, Juan L. Mendoza

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Cryo-EM structure of the calcium-sensing receptor complexed with the kokumi substance γ-glutamyl-valyl-glycine by Hiroki Yamaguchi, Seiji Kitajima, Hiroshi Suzuki, Shota Suzuki, Kouki Nishikawa, Akiko Kamegawa, Yoshinori Fujiyoshi, Kazutoshi Takahashi, Uno Tagami, Yutaka Maruyama, Motonaka Kuroda, Masayuki Sugiki

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Cryo-EM structure of Sudan ebolavirus glycoprotein complexed with its human endosomal receptor NPC1 by Fan Bu, Gang Ye, Hailey Turner-Hubbard, Morgan Herbst, Bin Liu, Fang Li

“…The findings reveal that the SUDV glycoprotein binds significantly more strongly to hNPC1 than the EBOV glycoprotein. Using cryo-EM, we determined the structure of the SUDV glycoprotein/hNPC1 complex, identifying four key residues in the SUDV glycoprotein that differ from those in the EBOV glycoprotein and influence hNPC1 binding: Ile79, Ala141, and Pro148 enhance binding, while Gln142 reduces it. …”
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CryoEM structure and small-angle X-ray scattering analyses of porcine retinol-binding protein 3 by Vineeta Kaushik, Luca Gessa, Nelam Kumar, Matyáš Pinkas, Mariusz Czarnocki-Cieciura, Krzysztof Palczewski, Jiří Nováček, Humberto Fernandes

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Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM by Matthew Cook, Christian Freniere, Chunxiang Wu, Faith Lozano, Yong Xiong

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Mutations in human prion-like domains: pathogenic but not always amyloidogenic by Andrea Bartolomé-Nafría, Javier García-Pardo, Salvador Ventura

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The Structural, Biophysical, and Antigenic Characterization of the Goose Parvovirus Capsid by Korosh Jabbari, Mario Mietzsch, Jane Hsi, Paul Chipman, Jianming Qiu, Robert McKenna

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Unveiling the Molecular Architecture of HBV Spherical Subviral Particles: Structure, Symmetry, and Lipid Dynamics by Sonal Garg, Alyssa Ochetto, Jianming Hu, Joseph Che-Yen Wang

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Molecular determinants of Neu5Ac binding to a tripartite ATP independent periplasmic (TRAP) transporter by Parveen Goyal, KanagaVijayan Dhanabalan, Mariafrancesca Scalise, Rosmarie Friemann, Cesare Indiveri, Renwick CJ Dobson, Kutti R Vinothkumar, Subramanian Ramaswamy

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Structural basis of FpGalNase and its combination with FpGalNAcDeAc for efficient A-to-O blood group conversion by Meiling Zhou, Kaishan Luo, Chao Su, Yan Sun, Zuyan Huang, Shuo Ma, Xun Gao, Jiwei Wang, Chen Zhang, Pengcheng Han, Guoqiu Wu

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Anti-Idiotypic Antibody as a Booster Vaccine Against Respiratory Syncytial Virus by Shreya Mukhopadhyay, Ioannis Manolaridis, Christopher Warren, Aimin Tang, Gregory O’Donnell, Bin Luo, Ryan P. Staupe, Kalpit A. Vora, Zhifeng Chen

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