Genetic diversity of Plasmodium falciparum reticulocyte binding protein homologue-5, which is a potential malaria vaccine candidate: baseline data from areas of varying malaria end... by Angelina J. Kisambale, Dativa Pereus, Salehe S. Mandai, Beatus M. Lyimo, Catherine Bakari, Gervas A. Chacha, Ruth B. Mbwambo, Ramadhan Moshi, Daniel A. Petro, Daniel P. Challe, Misago D. Seth, Rashid A. Madebe, Rule Budodo, Sijenunu Aaron, Daniel Mbwambo, Abdallah Lusasi, Stella Kajange, Samwel Lazaro, Ntuli Kapologwe, Celine I. Mandara, Deus S. Ishengoma

“…A total of 27 haplotypes were observed among the 313 monoclonal samples and under-fives exhibited higher haplotype counts. The Pf3D7 was detected as Hap_1, which occurred in 16/313 (5.1%) monoclonal sequences. …”
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Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model by Tala Curry-Koski, Tala Curry-Koski, Liam P. Curtin, Mitra Esfandiarei, Mitra Esfandiarei, Mitra Esfandiarei, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas, Theresa Currier Thomas

“…Glut1 immunostaining was used to quantify microvascular density, IgG staining to assess BBB permeability, and microglial counts to evaluate neuroinflammation.ResultsUsing Glut1 staining, 6M-MFS mice and 12M-CTRL similarly present decreased microvascular density in the dentate gyrus (DG), cornu ammonis 1 (CA1), and cornu ammonis 3 (CA3) regions of the hippocampus. 6M-MFS mice exhibit increased BBB permeability in the DG and CA3 as evident by Immunoglobulin G (IgG) staining. …”
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plaTform fOr Urinary tract infection diagnostiC evAluatioN (TOUCAN): a protocol for a prospective diagnostic accuracy study of point-of-care testing in patients suspected of acute... by Thomas R Fanshawe, Philip J Turner, Gail N Hayward, Margaret Glogowska, Alastair D Hay, Michael Moore, Mark Lown, Christopher C Butler, Mandy Wootton, Mark H Wilcox, Jane Freeman, Nicola Kenealy, Valerie Tate, Owain Llion, Rebecca Lowe

“…The UTI reference standard culture, although based on the national methods, is modified to provide accurate bacterial counts, better to define a microbiological diagnosis of UTI. …”
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Yeast cell wall polysaccharides accelerate yet in-feed antibiotic delays intestinal development and maturation via modulating gut microbiome in chickens by Fangshen Guo, Jianing Qiao, Zeqiong Hu, Jia Huang, Ruichen Bi, Waseem Abbas, Wenrui Zhen, Yuming Guo, Zhong Wang

“…Also, BMD markedly downregulated gene expression levels of β-catenin, lysozyme, occludin and FABP-2 (d 21) and innate immune related genes CD83 and MHC-I mRNA levels (d 42, P < 0.05), and decreased goblet cell counts in the ileum of chickens (d 21 and d 42, P < 0.05). …”
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Hubungan Kadar LDL dan HDL Serum Ibu Hamil Aterm dengan Berat Lahir Bayi by Oktalia Sabrida, Hariadi ., Eny Yantri

“…Examination of LDL and HDL serum level to 31 term pregnancy sample choose by consecutive sampling, and then infant’s birth weight was counted within 1 hour after birth without clothes. …”
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Growth performance of neonatal Holstein heifers fed acidified waste milk containing essential oil blend and encapsulated butyrate alone or in combination by Ting Liu, Zhihao Luo, Peng Li, Shuru Cheng, Jianping Zhu, David.P. Casper

“…Heifers fed EO demonstrated lower fecal Salmonella counts compared with heifers fed CON, C4, and E4. …”
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Anti-Inflammatory Effects of Cerium Dioxide Nanoparticles on Peritonitis in Rats Induced by Staphylococcus epidermidis Infection by Yan Li, Hongmei Sun, Zhiqi Yin, Xuexi Guo, Jun Yan

“…The CeO2 has an average size of 37 ± 3 nm with binding activity to proteins, phenolic compounds, and alkaloids. After counting the white blood cells in the peritoneal lavage fluid, it was found that the total number of white blood cells and the percentage of neutrophils in the model group were significantly increased (both P<0.05), and CeO2 treatment significantly reversed the above changes (both P<0.05). …”
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Differences in Optical Two-dimensional Morphological Characteristics of Downward Lightning Channels with Different Polarities by Mei Ji, Lü Weitao, Qi Qi, Xu Weiqun, Wu Bin, Ma Ying, Liu Hengyi, Chen Lüwen, Zhang Yang

“…Moreover, there are significant differences in the tilt angles and directional turning counts of the return stroke channels between positive and negative CG lightning. …”
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ART Regimen and Other Sociodemographics Do Not Affect Cytokine Expression in HIV Patients in Ghana by Samuel Essien-Baidoo, Dorcas Obiri-Yeboah, Yeboah Kwaku Opoku, Elvis Ayamga, Kevin Hodi Zie, Daniel Attoh, Evans Obboh, Anna Hayfron Benjamin, Justice Afrifa

“…Age significantly correlated inversely with IFN-γ (r = −0.27, p≤0.001), IL-10 (r = −0.24, p≤0.001), and IL-12 (r = −0.18, p=0.01) while duration on ART significantly correlated inversely with IFN-γ (r = −0.16, p=0.02). CD4 counts at 6 months and 12 months on ART correlated inversely with IL-17 (r = −0.17, p=0.02) and plasma viral load at 1 year (r = −0.22, p≤0.001), respectively. …”
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Comparison of the Accuracy of Four Malaria Diagnostic Methods in a High Transmission Setting in Coastal Cameroon by Marcel N. Moyeh, Innocent M. Ali, Dieudonné L. Njimoh, Akindeh M. Nji, Palmer M. Netongo, Marie S. Evehe, Barbara Atogho-Tiedeu, Stephen M. Ghogomu, Wilfred F. Mbacham

“…SD Bioline™ had a diagnostic sensitivity of 91.80% [95%CI: 81.90-97.28] at a parasitaemia of less than 500 parasites/μl of blood but a sensitivity of 100% for parasite counts above 500 parasites/μl of blood. The predictive values of the positive test were highly comparable between light microscopy (90.09%, [95%CI: 83.61-94.18]) and SD Bioline™ mRDT (90.91%, [95%CI: 84.50-94.83]), P=0.98 with kappa values of 0.898 but lower for presumptive diagnosis (50.89%, [95%CI: 43.72-58.03]), P<0.0001, and kappa value of 0.277. …”
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Role of Cylindrospermopsis sp. in vertical nitrogen changes observed in tropical oxidation wastewater treatment ponds by M. Srichomphu, O. Phewnil, T. Pattamapitoon, Ratcha Chaichana, K. Chunkao, W. Wararam, N. Dampin, P. Maskulrath

“…In the density of phytoplankton which were collected by measuring 20 liters of water and filtered using a 36-micron plankton net, calculated and counted under a high magnification microscope, as the species are classified according the taxonomy.FINDINGS: The results of the wastewater quality were as follows: the content of suspended solids was 65–81 milligram per liter, water temperature was 31.8–33.2°C, potential of hydrogen was 8.7–9.2, total nitrogen content was 4.0–5.3 milligram per liter, ammonium content was 0.03–0.06 milligram per liter, nitrate content was 0.09–0.12 milligram per liter, total phosphorus content was 0.9–1.3 milligram per liter, and phosphate content was 0.4–0.5 milligram per liter. …”
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Perbedaan Sensitivitas Kuman Pseudomonas Aeruginosa Penyebab Infeksi Nosokomial Terhadap Beberapa Antibiotika Generik dan Paten by Ayu Andrian Putri, Roslaili Rasyid, Rahmatini .

“…Djamil Padang that determinded by simple random sampling formula and used Kirby-Bauer diffusion method then counted the free zone germ towards 4 antibiotics generic and branded name. …”
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An Arctic sea ice concentration data record on a 6.25&thinsp;km polar stereographic grid from 3 years of Landsat-8 imagery by H.-S. Jung, S.-M. Lee, S.-M. Lee, J.-H. Kim, K. Lee

“…The classified data were projected onto a 6.25 km polar stereographic grid, and SIC for each grid cell was obtained by counting ice-classified pixels within the grid. SIC was only computed for grid cells where more than 99 % of their area was covered with Landsat-8 pixels to limit the uncertainty in SIC arising from grids that are not fully concentrated with Landsat-8 pixels. …”
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Association of systemic immune-inflammatory index with in-stent restenosis in patients with and without diabetes mellitus by Xunwei Deng, Xunwei Deng, Xunwei Deng, Qiaoting Deng, Qiaoting Deng, Qiaoting Deng, Qunji Zhang, Qunji Zhang, Qunji Zhang, Jingyuan Hou, Jingyuan Hou, Jingyuan Hou

“…The SII was calculated based on neutrophil, platelet and lymphocyte counts. Multivariable logistic regression models were employed to assess the associations between SII and ISR prevalence. …”
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Determining nutritional indices of Dori bleak, Alburnus doriae and Brond Snout, Chondrostoma regium in Zayandehroud River, Central Iran by Omidvar Farhadian, JAVAD BAGHERI, Nasrollah Mahboobi Soofiani, Safiollah Heidari, Eisa Ebrahimi

“…After separation, the eaten food items were identified and classified, counted, and weighed at the lowest possible systematic level using identification keys of aquatic invertebrates. …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…We found that gga-miR-6634-5p significantly down-regulated the mRNA levels of proliferation-related genes (CCND1, CDK1, and CDK6), decreased cell viability, the number of EdU-labelled positive cells, and the percentage of S-phase cells, as analysed by quantitative real time PCR (qRT-PCR), cell counting kit-8 (CCK-8), 5-ethynyl-2′deoxyuridine (EdU) and flow cytometry analyses (P < 0.01 or P < 0.05). qRT-PCR and enzyme-linked immunosorbent assay (ELISA) results demonstrated that gga-miR-6634-5p up-regulated the expression of steroid synthesis-related genes (CYP19A1, 3β-HSD, StAR and FSHR) (P < 0.01 or P < 0.05), as well as the secretion of estradiol (E2) and progesterone (P4) (P < 0.01 or P < 0.05). …”
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Non-Immersive Virtual Reality Exercise Can Increase Exercise in Older Adults Living in the Community and in Long-Term Care: A Randomized Controlled Trial by Sheehy L, Bharadwaj L, Nissen KA, Estey JL

“…Physical testing and questionnaires addressed objective 1). Counts for objectives 2) and 3) were reported by the participants and retrieved from the non-immersive virtual reality platform. …”
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Regulatory role of lnc-MAP3K13-3:1 on miR-6894-3p and SHROOM2 in modulating cellular dynamics in hepatocellular carcinoma by Kuai Chen, Manqin Zhu, Qinghua Hu, Hui Huang, Ka Chen, Xia Shuai, Jinshi Huang, Qiang Tao, Zhibin Guo

“…Cell proliferation was analyzed using the cell counting Kit-8 assay, while flow cytometry was used to assess cell cycle distribution and apoptosis. …”
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Navigating the Ice: Establishing Pre-Concussion Baselines in Ice-Hockey Players for Gait Assessment Utilizing Inertial Measurement Units by Julia Müller, Noah Köppel, Patrick Eggenberger, Emanuel Brunner, Thomas Weber, Eling D. de Bruin

“…., preferred gait speed whilst counting backwards. Application of the dual-task paradigm aimed at quantifying the automaticity of movement (Soulard et al., 2021). …”
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Features of expression of immunohistochemical markers in local and generalized clear cell kidney cancer by E. M. Frantsiyants, A. N. Shevchenko, E. A. Dzhenkova, N. S. Karnaukhov, T. A. Gudtskova, A. A. Breus, E. V. Filatova, D. A. Shvyrev

“…The results of the reactions with markers were evaluated by counting the number of colored cells in each 3rd field of view of the entire drug at magnification of the X200 lens in the AXIO Scope microscope. …”
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