Inhibitory Mechanism of Robiotic Bacteria on The Growth of Vibrio harveyi in Tiger Shrimp (Penaeus monodon) Larvae by . Widanarni, E. Ayuzar, . Sukenda

“…The inhibitory effect of SKT-b,1Ub, and Ua on the growth of V. harveyi was investigated by concomitant incubation of the two bacteria in a culture shrimp larvae. Colony forming unit of V. harveyi, probiotic and total of bacteria in dead, live larvae and water culture was monitored, and survival rate of larvae was investigated. …”
Get full text

Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Tr... by Andrzej Eljaszewicz, Lukasz Bolkun, Kamil Grubczak, Malgorzata Rusak, Tomasz Wasiluk, Milena Dabrowska, Piotr Radziwon, Wojciech Marlicz, Karol Kamiński, Janusz Kloczko, Marcin Moniuszko

“…ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF). To date, the effects of G-CSF treatment on the level of mobilization of different stem and progenitor cells in ALL patients subjected to clinically effective chemotherapy have not been fully elucidated. …”
Get full text

TOM40 as a prognostic oncogene for oral squamous cell carcinoma prognosis by Lifei Deng, Hong Ran, Dunhui Yang, Zhen Wang, Peng Zhao, Hengjie Huang, Yongjin Wu, Peng Zhang

“…The TCGA-OSCC cohort was divided into the TOM40 high and TOM40 low groups and the correlation between TOM40 expression level and the sensitivity to frequently used chemotherapeutic drugs was evaluated. CCK-8 and colony formation assays were applied to determine the cell growth. …”
Get full text

The impact of dimethylaminohexadecyl methacrylates on the physical and antibacterial properties of endodontic sealers by Faisal Alharamlah, Fawaz AlTuwaijri, Haitham AlQuorain, Abdul Samad Khan, Faisal Alonaizan, Rashed Alsahafi, Michael D. Weir, Hockin H. K. Xu, Abdulrahman A. Balhaddad

“…The antibacterial properties were assessed by determining the number of colony-forming units (CFUs) of Enterococcus faecalis and scanning electron microscopy (SEM). …”
Get full text

Analyse des terres cuites architecturales de l’aqueduc de Nyon/Colonia Iulia Equestris (Suisse) by Benjamin Clément, Christophe Henny, Anne Schmitt

“…In the absence of dating evidence for the construction itself, the establishment of public baths near the colony’s forum at the beginning of the 1st century AD provides us with a chronological clue to its construction. …”
Get full text

Asymptomatic Bacteriuria among Pregnant Women in Addis Ababa, Ethiopia: Prevalence, Causal Agents, and Their Antimicrobial Susceptibility by Ketema Bizuwork, Haile Alemayehu, Girmay Medhin, Wondwossen Amogne, Tadesse Eguale

“…Women whose urine samples carried greater than or equal to 105 colony-forming units (CFU) of bacteria per milliliter of urine when grown on plate count agar were considered positive for ASBU. …”
Get full text

Comparison of phenotypic and genetic traits of ESBL-producing UPEC strains causing recurrent or single episode UTI in postmenopausal women by Michelle Kalu, Peter Jorth, Annie Wong-Beringer

“…Presence of hlyA, ompT, and fimH genes were confirmed by colony PCR, and qRT-PCR was performed using extracted RNA from a subset of 18 strains (12 recurrent; 6 single episode) grown in Luria-Bertani media and isolated from infected BECs to characterize gene expression. …”
Get full text

Effect of Zirconium Oxide and Zinc Oxide Nanoparticles on Physicochemical Properties and Antibiofilm Activity of a Calcium Silicate-Based Material by Juliane Maria Guerreiro-Tanomaru, Adinael Trindade-Junior, Bernardo Cesar Costa, Guilherme Ferreira da Silva, Leonardo Drullis Cifali, Maria Inês Basso Bernardi, Mario Tanomaru-Filho

“…The analysis was conducted by using the number of colony-forming units (CFU/mL). The compressive strength was performed in a mechanical testing machine. …”
Get full text

Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro by Eduardo Branco de Sousa, Gilson Costa dos Santos Junior, Ramon Pinheiro Aguiar, Rafaela da Costa Sartore, Ana Carolina Leal de Oliveira, Fabio Ceneviva Lacerda Almeida, Vivaldo Moura Neto, Diego Pinheiro Aguiar

“…Samples from OA patients formed a higher number of colonies than the ones from non-OA patients. After 21 days, the colony-forming cells from OA patients differentiated into the three mesenchymal cell lineages, under the appropriated induction protocols. …”
Get full text

Refractory myasthenia gravis treated with autologous hematopoietic stem cell transplantation by Benjamin Beland, Jan Storek, Liam Quartermain, Christopher Hahn, C. Elizabeth Pringle, Pierre R. Bourque, Michael Kennah, Natasha Kekre, Christopher Bredeson, David Allan, Kareem Jamani, Christopher White, Harold Atkins

“…Stem cells were mobilized with cyclophosphamide and granulocyte colony‐stimulating factor. The grafts were depleted of immune cells by selecting CD34+ cells. …”
Get full text

Rescue of in vitro models of CSF1R-related adult-onset leukodystrophy by iluzanebart: mechanisms and therapeutic implications of TREM2 agonism by Kelley C. Larson, Frederick W. Gergits, Abigail J. Renoux, Elizabeth J. Weisman, Borislav Dejanovic, Liyue Huang, Bhaumik Pandya, Donald G. McLaren, Berkley A. Lynch, Richard Fisher, Evan Thackaberry, David Gray, Francois Gaudreault, Christian Mirescu

“…CSF1R-ALSP is caused by heterozygous loss-of-function mutations in the colony stimulating factor 1 receptor (CSF1R) gene, which encodes a receptor required for the differentiation of myeloid cells, as well as for microglial survival and proliferation. …”
Get full text

Unveiling the Potency of Gardenia Extract Against <i>H. pylori</i>: Insights from In Vitro and In Vivo Studies by Pornpen Werawatganone, Duangporn Werawatganon, Nattida Noonak, Maneerat Chayanupatkul, Tanittha Chatsuwan, Naruemon Klaikeaw, Walaisiri Muangsiri, Prasong Siriviriyakul

“…Thirty-five male Sprague–Dawley rats were inoculated with <i>H. pylori</i> (10⁸–10¹⁰ colony-forming unit/mL) and divided into five groups. …”
Get full text

Clinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma by Liuyan Chen, Lvying Wu, Minying Tang, Yuanhang Cheng, Kuanyin Wang, Jianan Zhang, Wenyi Deng, Lingfeng Zhu, Jin Chen

“…Various assays, including assessments of cell proliferation, apoptosis, the cell cycle, cell migration and invasion, and colony formation, and xenograft tumor models were subsequently performed following to the knockdown of DBF4 expression via shRNA. …”
Get full text

CXCR4 promotes tumor stemness maintenance and CDK4/6 inhibitors resistance in ER-positive breast cancer by Qiangfeng Shi, Wang Yang, Yiye Ouyang, Yujie Liu, Zijie Cai

“…The effects of CXCR4 knockdown and overexpression via siRNAs and plasmids transfection were examined by mammosphere formation, RT-qPCR, flow cytometry, MTT and colony formation assays. The regulation mechanisms were analyzed by RT-qPCR, western blotting and immunofluorescence experiments. …”
Get full text

Characterization of subcutaneous and visceral de-differentiated fat cells by Yan Li, Houyu Zhang, Carlos F. Ibáñez, Meng Xie

“…We also show that high-fat diet (HFD) feeding enhances DFAT cell colony formation and re-differentiation into adipocytes, while switching from HFD to chow diet (CD) only reverses their re-differentiation. …”
Get full text

The effect of smoking on the immediate effectiveness of induction chemotherapy in the combined treatment of HPV-associated oropharyngeal cancer by P. V. Golubev, L. V. Bolotina, A. R. Gevorkov, T. I. Deshkina

“…Patients in both groups received prophylactic granulocyte colony-stimulating factor. At the second stage, standard chemoradiotherapy was carried out against the background of drug radiomodification with carboplatin. …”
Get full text

Cultivatable Bacteriota of Chronic Wound of Patients with Diabetic Foot Syndrome with Critical Limb Ischemia Based on Wound Biopsy in Peri-Revascularization Period by Syedah Sarah Hussaini, Fran Dominic Grubesa, Mateusz Gajda, Martyna Schönborn, Katarzyna Bogucka, Mikołaj Maga, Paweł Maga, Jadwiga Wójkowska-Mach

“…The control parameters of blood supply improved; TCPO2 was 38.5 mmHg, the ABI was 0.9, and the TBI was 0.3, with a reduction in the average WIfI score to 3.7 (mild), while total colony-forming units (CFUs) increased by 13.5%. No cases of reocclusion or restenosis were observed during the study; however, small amputations were performed in two patients (8.7%). …”
Get full text

Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption by Yi Zhang, Yongding He, Meixin Yan, Shilong Zhang, Sisi Zhou, Wankuan Shen

“…On YePSA medium, the gene SsMFSST1 does not affect the growth, spore morphology, colony morphology, and oxidative stress ability of the haploid spores of S. scitamineum. …”
Get full text

Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig by Kunhong Xie, Weidong Cai, Lingjie Li, Bing Yu, Yuheng Luo, Zhiqing Huang, Xiangbing Mao, Jie Yu, Ping Zheng, Hui Yan, Hua Li, Jun He

“…From 1 to 15 d, the CA and DA groups were orally fed with 1.0 × 1011 colony-forming units A. muciniphila per day, while the CON and DCON groups were received gastric infusion of anaerobic sterile saline per day. …”
Get full text

Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy by Gregory B. Lesinski, Chrystal M. Paulos, Maggie Phillips, Jacklyn Hammons, Isaac Karpovsky, Zaid Mahdi, Natalie K. Horvat, Megan M. Wyatt, Margaret A. Hall, Cameron J. Herting, Richard A. Moffitt

“…However, these models have limitations, including marked tumor variability across individual mice and the cost of colony maintenance.Methods Using flow cytometry and immunohistochemistry, we characterized the immunological features and trafficking patterns of adoptively transferred T cells in orthotopic PDAC (C57BL/6) models using two mouse cell lines, KPC-Luc and MT-5, isolated from C57BL/6 KPC-GEMM (KrasLSL-G12D/+p53−/− and KrasLSL-G12D/+p53LSL-R172H/+, respectively).Results The MT-5 orthotopic model best recapitulates the cellular and stromal features of the TME in the PDAC GEMM. …”
Get full text