Exosomal PVRL4 Promotes Lung Adenocarcinoma Progression by Enhancing the Generation of Myeloid‐Derived Suppressor Cell‐Secreted TGF‐β1 by Yahai Liang, Jinmei Li, Lihua Zhang, Jinling Zhou, Meilian Liu, Xiaoxia Peng, Weizhen Zheng, Zhennan Lai

“…Methods Detection of levels of mRNAs and proteins was conducted using qRT‐PCR and western blotting. The CCK‐8, colony formation, transwell, wound healing assays, and flow cytometry were used to explore cell growth, invasion, migration, and apoptosis, respectively. …”
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Endoplasmic reticulum stress-related CLIP4 plays a procarcinogenic role in hepatocellular carcinoma: an integrated analysis by Anqi Wang, Sitong Yan, Weijia Jiang, Xiang Chen, Yuhan Huang, Xiangyu Zu, Xiao Du, Lulu Fan, Jiatao Liu, Guoping Sun

“…Public databases and miRNA-seq data were used to explore the TF-miRNA-CLIP4 regulatory network. CCK-8, colony formation, EdU staining, wound-healing, Transwell, western blotting and RT-qPCR were used to detect the effects of CLIP4 on the proliferation, migration and epithelial-mesenchymal transition (EMT) of HCC cells. …”
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Identification and validation of CDK1 as a promising therapeutic target for Eriocitrin in colorectal cancer: a combined bioinformatics and experimental approach by Jiemiao Shen, Xing Gong, Haili Ren, Xia Tang, Hairong Yu, Yilu Tang, Shen Chen, Minghui Ji

“…GO and KEGG enrichment analyses were performed to clarify the functional roles of CDK1-related genes. CCK-8, colony formation, cell migration, cell invasion, and wound healing assays were employed to explore tumor-promoting role of CDK1. …”
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Therapy of genomic unstable solid tumours (WHO grade 3/4）in clinical stage III/IV using individualised neoantigen tumour peptides-INP trial (individualised neoantigen tumour peptid... by Ling Wang, Xia Chen, Juan Zhao, Jiaxi Tang, Wanyan Tang, Bin Liao, Weiqi Nian

“…Patients will be treated with their own individualised neoantigen polypeptide combined with a polypeptide adjuvant (human granulocyte-macrophage colony-stimulating factor). The primary endpoint is safety indicators, including general and specific adverse events which will be monitored continuously. …”
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Effect of ultrasonically-activated irrigation protocols used for regenerative endodontics on removal of dual species biofilm in a three-dimensionally printed tooth model: in vitro... by Saeed Mustafa, Marwa A. Meheissen, Sybel Moussa, Rania ElBackly

“…Bacterial reduction was determined by colony-forming unit (CFU) counting (n = 12/G), whilst biofilms were analyzed using field emission scanning electron microscopy in additional samples. …”
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RETRACTED ARTICLE: LRPPRC regulates malignant behaviors, protects mitochondrial homeostasis, mitochondrial function in osteosarcoma and derived cancer stem-like cells by Ziyi Zhao, Yingwei Sun, Jing Tang, Yuting Yang, Xiaochao Xu

“…Results we found that LRPPRC was highly expressed in several osteosarcoma cell lines and that LRPPRC knockdown inhibited malignant behaviors, including proliferation, invasion, colony formation and tumor formation, in MG63 and U2OS cells. …”
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Distribution of Aeromonas bacterial population in water, sediment and Nile tilapia in fish culture pond, Guder, Ethiopia by Bekele Lema, Natarajan Pavanasam, Kassaye Balkew, Zufan Bedewi

“…The level of bacterial population in water, sediment and fish tissue were done by following standard methods and expressed as colony-forming units (CFU) in water per milliliter (CFU ml-1), sediment and fish tissues(CFU g-1).The results revealed more bacterial population in sediment (3.43 x106 to 5.54 x 106 CFU g-1) than in water (1.45x106 to 4.0x106 CFU ml-1) and  fish tissues (1.06 ± 1.10x104 to 1.74 ± 10.8x104 CFU g-1in gill filaments; 1.62 ± 11.2x104 to 2.82 ± 13.0x104 CFU g−1 in intestine from; and 0.82 ± 5.9x104 to 1.60 ± 12.1.6x104 CFU g-1; in kidney from 0.48 ± 5.0 to 0.77 ± 4.1x104 CFU g-1. in skin). …”
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Mesenchymal Stem/Stromal Cells Derived from Induced Pluripotent Stem Cells Support CD34pos Hematopoietic Stem Cell Propagation and Suppress Inflammatory Reaction by Mohsen Moslem, Irina Eberle, Iuliia Weber, Reinhard Henschler, Tobias Cantz

“…Considering functional capabilities, iPSC-MSCs provided supportive feeder layer for CD34+ hematopoietic stem cells’ self-renewal and colony forming capacities. Furthermore, iPSC-MSCs gained immunomodulatory function to suppress CD4+ cell proliferation, reduce proinflammatory cytokines in mixed lymphocyte reaction, and increase regulatory CD4+/CD69+/CD25+ T-lymphocyte population. …”
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Cytokines and Water Distribution in Anorexia Nervosa by Hubertus Himmerich, Bethan Dalton, Olivia Patsalos, Ulrike Schmidt, Iain C. Campbell

“…Following blood collection, 27 cytokines and chemokines were quantified using multiplex ELISA-based technology: Eotaxin, Eotaxin-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon- (IFN-) γ, interleukin- (IL-) 1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, interferon γ-induced protein- (IP-) 10, macrophage inflammatory protein- (MIP-) 1α, MIP-1β, monocyte chemoattractant protein- (MCP-) 1, MCP-4, thymus and activation-regulated chemokine (TARC), TNF-α, and TNF-β. …”
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Antimicrobial susceptibility patterns of pathogens isolated from clinical specimens at a tertiary care hospital in Bangladesh, 2020–2023 by Mohammad Kamruzzaman, Fahim Alam Nobel, Saiful Islam, Amiyo Haider, Mohammad Nasir Uddin, Mohammod Johirul Islam

“…The isolates were characterized through colony morphology, gram staining, and biochemical assays. …”
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lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis by YiFan Wang, GuanYin Zhu, Fang Pei, ZhiHe Zhao

“…The expression of SNHG15 was increased in THP-1 cells stimulated by macrophage colony-stimulating factor (M-CSF)/receptor activator of nuclear factor-kappa B ligand (RANKL). …”
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DLAT is involved in ovarian cancer progression by modulating lipid metabolism through the JAK2/STAT5A/SREBP1 signaling pathway by Hui Wang, Shen Luo, Yue Yin, Yang Liu, Xiaomei Sun, Ling Qiu, Xin Wu

“…The effects of DLAT silencing on changes in lipid metabolism, cell viability, migration, and invasion were examined in SKOV3 and OVCAR3 cells using CCK-8, colony formation, Transwell migration and invasion, and wound-healing assays. …”
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The quality and technological parameters of milk obtained from dairy cows with subclinical mastitis by Magdalena Zalewska, Paulina Brzozowska, Magdalena Rzewuska, Ewelina Kawecka-Grochocka, Daria M. Urbańska, Tomasz Sakowski, Emilia Bagnicka

“…However, fixed linear regression indicated that the number of colony-forming units (cfu/mL) only had a significant influence on FPD and clotting time, and as such, the number of bacteria in a sample did not influence milk yield or quality during subclinical chronic mastitis.…”
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Antimicrobial Resistance Patterns of Bacterial Isolates from Blood Culture among HIV/AIDS Patients at Felege Hiwot Referral Hospital, Northwest Ethiopia by Mohabaw Jemal, Teshiwal Deress, Teshome Belachew, Yesuf Adem

“…Identification was performed using colony characteristics and standard biochemical techniques. …”
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Succinate supplementation alleviates liver cancer by inhibiting the FN1/SQLE axis-mediated cholesterol biosynthesis by Shuyuan Chang, Ayaka Tomii, Yunfei Zhou, Xun Yang, Yihong Dong, Jun Yan, Aodi Wu, Yumeng Wang, Qingxin Zhang, Hongxue Meng, Lei Yu, Wei Sun, Dabin Liu

“…Succinate supplementation exhibits an anti-tumorigenic effect, inhibiting cell proliferation and colony formation in liver cancer cells but not in non-tumor LO2 cells. …”
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A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis chall... by S.A.S. van der Klein, M. Bernardeau, Q. Wang, K. Gibbs

“…Treatments in each trial were a non-challenged control (C); challenged control (10 x dose of Eimeria/bird on d 14 and 1.0 x ∼109 colony forming units (CFU)/bird of C. perfringens on d 16-20; CC); and CC supplemented daily via the waterline with 1 × 109 CFU/bird of probiotic (CC+Probiotic). …”
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Nonfimbrial Adhesin Mutants Reveal Divergent Escherichia coli O157:H7 Adherence Mechanisms on Human and Cattle Epithelial Cells by Matthew R. Moreau, Indira T. Kudva, Robab Katani, Rebecca Cote, Lingling Li, Terrance M. Arthur, Vivek Kapur

“…Supershedding (SS) is a phenomenon that has been reported in some cattle that shed more than 104 colony-forming units of O57:H7 per gram of feces, 100–1000 times more or greater than normal shedders. …”
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Assessment of the Heterotrophic Bacterial Contamination in Waterlines of Dental Units as a Potential Health Risk Factor in Occupational and Patient’s Exposures by Ali Shahryari, Amineh Zahed, Abdolhalim Rajabi, Maryam Shafipour, Niloufar Emrani, Hadi Rahimzadeh, Hamidreza Heidari

“…Bacterial contamination in the waterlines, dental handpieces, and other water-related components of dental units was investigated and analyzed in terms of Colony-Forming Units per milliliter (CFU/ml). The data were analyzed using the Kruskal-Wallis and Wilcoxon signedrank tests using SPSS version 23.Results: The mean and standard deviation of bacterial contamination across all studied departments were significantly higher than the recommended limit of 500 CFU/ml (ranging from 10,250.00±8,924.28 CFU/ml for the inlet to the water tank in the pediatric department to 275,625.00±41,520.86 CFU/ml for the air-water syringe before flushing in the surgery department). …”
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Antimicrobial Susceptibility Testing of Metronidazole and Clindamycin against Gardnerella vaginalis in Planktonic and Biofilm Formation by Ting Li, Zhan Zhang, Fengjuan Wang, Yuanhui He, Xiaonan Zong, Huihui Bai, Zhaohui Liu

“…G. vaginalis isolates were obtained from the vagina and identified using their characteristic colony morphology. Sequence data of clinical G. vaginalis isolates were confirmed by comparing 16S rDNA sequences. …”
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MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells by Hou Lingli, Liu Qi, Zhao Ying, Yang Hongwei, Meng Qingying, Yu Fei

“…They consist of the cell proliferation assay, wound healing assay, transwell assay, colony formation assays and qRTPCR. Expression levels of target proteins were determined using Western blotting. …”
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