Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage by Yuan Jiang, Albert Wingnang Leung, Heyu Hua, Xiancai Rao, Chuanshan Xu

“…Staphylococcus aureus was incubated with the different concentrations of curcumin for 60 min and then irradiated by blue light with the wavelength of 470 nm and with light dose of 3 J/cm2. The colony forming unit assay was used to investigate photocytotoxicity of curcumin on Staphylococcus aureus, confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) for assaying membrane permeability, FCM analysis with DCFH-DA staining for measuring the intracellular ROS level, and transmission electron microscopy (TEM) for observing morphology and structure. …”
Get full text

Evaluation of anticancer potency of Cu-isatin-1-(2-pyridyl) piperazine thiosemicarbazone (Cu-Istpypz) against epidermoid carcinoma cell line (A431) by Indranil Banerjee, Piyush Kumar, Narendra K. Singh, Paras N. Yadav, Yuba Raj Pokharel

“…The compound Cu-Istpypz also suppressed the colony formation of A431 cells in a concentration-dependent manner, as demonstrated by the clonogenic assay. …”
Get full text

Downregulation of LncRNA SNHG7 Sensitizes Colorectal Cancer Cells to Resist Anlotinib by Regulating miR-181a-5p/GATA6 by Deng Pan, Kehe Chen, Ping Chen, Yu Liu, Yingying Wu, Jingning Huang

“…We performed the Cell Counting Kit-8 and Colony formation assay, flow cytometric analysis, RNA pull-down, RNA-binding protein immunoprecipitation assay, and Luciferase reporter assay to confirm the interaction among SNHG7, miR-181a-5p, and GATA6. …”
Get full text

Natural Disaster Risk Assessments for Pine Honey Apiaries in Muğla, Turkey by Fatih Sarı

“…On the other hand, due to the mountainous topography and high precipitation rate of Muğla, the province has a high rate of flood formation (20 per year), which threatens the hive sites by destroying the entire colony. In this study, Apiary Locations Risk Index (ALRI) was carried out to guide the insurance process for apiary locations by applying the Forest Fire Risk Index (FFRI) and the Flood Hazard Risk Index (FHRI). …”
Get full text

Identification of CHD1L as an Important Regulator for Spermatogonial Stem Cell Survival and Self-Renewal by Shan-Shan Liu, Yin-Shan Bai, Li Feng, Wen-Wei Dong, Yang Li, Li-Ping Xu, Ning-Fang Ma

“…Knockdown of endogenous Chd1l in cultured mouse undifferentiated SSCs inhibited the expression levels of Oct4, Plzf, Gfrα1, and Pcna genes, suppressed SSC colony formation, and reduced BrdU incorporation, while increasing SSC apoptosis. …”
Get full text

Immunogenicity Assessment of Lipegfilgrastim in Patients with Breast Cancer Receiving Chemotherapy by Linglong Zou, Anton Buchner, Martin Roberge, Patrick M. Liu

“…Lipegfilgrastim is a long-acting, once-per-cycle, glycopegylated recombinant granulocyte colony-stimulating factor (G-CSF) used to prevent neutropenia in patients receiving myelosuppressive chemotherapy. …”
Get full text

TMEM100 Modulates TGF-β Signaling Pathway to Inhibit Colorectal Cancer Progression by Huixia Li, Chuan Cheng, Weibo You, Jiujian Zheng, Jie Xu, Peng Gao, Jianping Wang

“…The role of TMEM100 in the progression of CRC cells was evaluated by MTT, colony formation, scratch healing, and Transwell assays. …”
Get full text

Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm by Andrea Freire, Victor Augusto Alves Bento, Eduardo Inocente Jussiani, Avacir Casanova Andrello, Maria Carolina Silva Marques

“…The loss of microhardness was assessed considering the initial microhardness as 100% on enamel, dentin, and composite resin substrates. Colony Forming Units (CFU/mL) were counted in 3 media. …”
Get full text

Successful Treatment of Cisplatin Overdose with Plasma Exchange by Yasuhiro Yamada, Yoshiaki Ikuta, Kisato Nosaka, Nobutomo Miyanari, Naoko Hayashi, Hiroaki Mitsuya, Hideo Baba

“…Here, we report a case of large cisplatin overdose, successfully managed by plasma exchange, intravenous hydration, granulocyte colony-stimulating factor (G-CSF) administration, and other supportive care. …”
Get full text

Improvement of Injera Shelf Life and Staling through Vacuum and Nonvacuum Polyethylene Packaging: Their Synergistic Effect with Chemical Preservative by Abinet Terefe, Shimelis Admasu Emire, Habtamu Fekadu Gemede, Ashagrie Z. Woldegiorgis

“…Among these, VP+ had the least microbial load (5.3∗101 & 9.0∗101 bacterial & yeast and mold colony forming unit (cfu)/g, respectively). But it was least effective regarding staling as it had the least average scoring of MC, pH, and L value (60.96%, 3.33, and 45.92, respectively) and sensory acceptability, basically due to the crumbling effect of the packaging method used. …”
Get full text

CCDC154 knockdown inhibits growth of liver cancer via suppressing expression of Snail by Rulan Ma, Hongmei Nie, Caijing Mo, Dawei Yuan, Kun Zhu, Kang Li

“…The proliferation and apoptosis of LC cells was evaluated by cell counting assay, colony formation assay and flow cytometry. The migration and invasion of LC cells were investigated using scratch wound-healing assay and Transwell assay. …”
Get full text

Hepatitis B e Antigen Seroconversion Is Related with the Function of Dendritic Cells in Chronic Hepatitis B Virus Infection by Chunjing Lin, Hai Zou, Shumin Wang

“…The peripheral blood mononuclear cells (PBMC) from 21 chronic HBV patients in immune tolerance state, 23 patients in inactive HBsAg carrier state, and 10 healthy HBV-naive blood donors were incubated and induced into DC in presence of granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4), respectively. …”
Get full text

Structural Characterization of Functional Ingredient Levan Synthesized by Bacillus siamensis Isolated from Traditional Fermented Food in Thailand by Nattapong Thakham, Supphasin Thaweesak, Nuttinee Teerakulkittipong, Natthiwut Traiosot, Autaipohn Kaikaew, Gary Antonio Lirio, Witawat Jangiam

“…Isolated colonies were characterized morphologically by gram staining methods. …”
Get full text

Parameter Acquisition Study of Mining-Induced Surface Subsidence Probability Integral Method Based on RF-AGA-ENN Model by Jinman Zhang, Liangji Xu, Jiewei Li, Yueguan Yan, Ruirui Xu

“…To obtain more accurate PIM parameters in the absence of observational data, we propose a combined machine learning model (RF-AGA-ENN)—random forest (RF) extracts the best combination of features as the input layer of Elman neural network (ENN); ant colony algorithm (ACO) and genetic algorithm (GA) are combined (called AGA) for the weights and thresholds of ENN optimization. …”
Get full text

Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J by Ke-Yan Loo, Loh Teng-Hern Tan, Jodi Woan-Fei Law, Priyia Pusparajah, Learn-Han Lee, Vengadesh Letchumanan

“…This study aimed to investigate the prevalence of MDR V. parahaemolyticus to provide insight into the current antibiotic resistance patterns of this pathogen and to determine potential anti-Vibrio properties of Streptomyces MUM 178J which was previously isolated from the soils of a mangrove forest in Sarawak, Malaysia. Colony morphology and toxR-assay indicated that all samples tested positive for V. parahaemolyticus. …”
Get full text

Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation by Luz M. Avila-Portillo, Fabio Aristizabal, Angela Riveros, Martin C. Abba, Diego Correa

“…., priming) before their therapeutic use. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that plays key roles in immune response and hematopoiesis modulation through direct effects on hematopoietic progenitors’ proliferation, survival, and mobilization. …”
Get full text

COMPARISON OF THE COMPLETE NUCLEOTIDE SEQUENCES OF MITOCHONDRIAL DNA FROM WISTAR AND OXYS RAT STRAINS MAINTAINED AT THE INSTITUTE OF CYTOLOGY AND GENETICS SB RAS by R. A. Maksyutov, E. V. Gavrilova, T. S. Nepomniashchikh, U. N. Rotskaya, P. S. Loshchenova, N. G. Kolosova, O. I. Sinitsyna, S. N. Shchelkunov

“…The comparative analysis showed that Wistar (ICG) rats had a significant number of nucleotide substitutions compared to rat strains derived from an outbred Wistar colony. This observation contradicts the conjectured relationship of Wistar (ICG) rats to the original Wistar rat stock or its descendants. …”
Get full text

Phase I Study of Anti-CD3 x Anti-Her2 Bispecific Antibody in Metastatic Castrate Resistant Prostate Cancer Patients by Ulka Vaishampayan, Archana Thakur, Ritesh Rathore, Nicola Kouttab, Lawrence G. Lum

“…The men received 2.5, 5 or 10 × 109 aATC per infusion with low dose interleukin-2 and granulocyte-macrophage colony stimulating factor. Results. There were no dose limiting toxicities, and there was 1 partial responder and 3 of 7 patients had significant decreases in their PSA levels and pain scores. …”
Get full text

Quorum sensing-mediated inter-specific conidial anastomosis tube fusion between Colletotrichum gloeosporioides and C. siamense by Nikita Mehta, Abhishek Baghela

“…Post CAT fusion, the resulting conidia gave rise to putative heterokaryotic progenies with variable colony characteristics as compared to their parental strains. …”
Get full text

Preclinical Evaluation of a Bone-Marrow Autograft Culture Procedure for Generating Lymphokine-Activated Killer Cells in Vitro by Hans-Georg Klingemann, Heather Deal, Dianne Reid, Connie J Eaves

“…Primitive hematopoietic cells were quantitated by measuring the number of in vitro colony-forming progenitors produced after five weeks in secondary Dexter-type LTC. …”
Get full text