Preclinical pharmaco-toxicological screening of biomimetic melanin-like nanoparticles as a potential therapeutic strategy for cutaneous melanoma by Iasmina Marcovici, Iasmina Marcovici, Raul Chioibas, Raul Chioibas, Istvan Zupko, Iulia Pinzaru, Iulia Pinzaru, Alina Moaca, Alina Moaca, Adriana Ledeti, Adriana Ledeti, Lucian Barbu-Tudoran, Lucian Barbu-Tudoran, Andreea Geamantan, Andreea Geamantan, Iasmina Predescu, Iasmina Predescu, Cristina Adriana Dehelean, Cristina Adriana Dehelean

“…The cytotoxic, anti-migratory, anti-clonogenic, pro-oxidant and pro-apoptotic properties of MEL-NPs were investigated in vitro by applying the MTT viability test, bright-field and immunofluorescence microscopy, DCFDA/H2DCFDA test, scratch assay, colony formation assay, and RT-qPCR. The irritant and anti-angiogenic effects were assessed in ovo on the vascularized chorioallantoic membrane (CAM).ResultsThe as-made MEL-NPs presented a spherical morphology, an average size of 85.61 nm, a broad UV-VIS absorption spectrum, and a strong antioxidant activity. …”
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Heterogeneous nuclear ribonucleoprotein C promotes non-small cell lung cancer progression by enhancing XB130 mRNA stability and translation by Qinrong Wang, Xuanjing Gou, Lingling Liu, Daolan Deng, Yan Zhao, Jianjiang Zhou, Yuan Xie, Yinhui Jiang, Jianglun Li, Jian Zhang, Ying Liu

“…The proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of these cells were detected through Real-time cellular analysis, adherent colony formation, wound healing assay, invasion assay, and Western blotting. …”
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NPRL2 gene therapy induces effective antitumor immunity in KRAS/STK11 mutant anti-PD1 resistant metastatic non-small cell lung cancer (NSCLC) in a humanized mouse model by Ismail M Meraz, Mourad Majidi, Renduo Song, Feng Meng, Lihui Gao, Qi Wang, Jing Wang, Elizabeth J Shpall, Jack A Roth

“…NPRL2-stable cells showed a substantial increase in colony-formation inhibition and heightened sensitivity to carboplatin. …”
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NALCN expression is down-regulated and associated with immune infiltration in gastric cancer by Xuewei Li, Xuewei Li, Na Wu, Chen Wang, Beibei Pei, Xiaoyan Ma, Jun Xie, Jun Xie, Wenhui Yang

“…Mechanistically, NALCN knockdown markedly enhanced cell proliferation, colony formation and migration while reducing apoptosis rates in AGS and GES-1 cells. …”
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Deciphering the therapeutic effects of Xiyanping injection: insights into pulmonary and gut microbiome modulation, SerpinB2/PAI-2 targeting, and alleviation of influenza a virus-in... by Tengwen Liu, Shuping Li, Xuerui Wang, Mingjiang Liu, Yuchen Wang, Jie Ying, Shuwen Zhang, Yan Lin, Ning Wang, Yungjing Bai, Lan Xie, Tengfei Chen, Quansheng Feng, Xiaolong Xu

“…XYP injection down-regulated the activity of malondialdehyde, and the levels of interleukin (IL)-1β, IL-5, IL-6, tumor necrosis factor-α, IL-18, IL-15, granulocyte colony-stimulating factor, IL-9, chemokine (C-C motif) ligand-5, and C-X-C motif chemokine ligand 5, while up-regulated the activities of glutathione peroxidase reactive and superoxide dismutase, and the level of interferon-γ. …”
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Generation of novel oncolytic vaccinia virus with improved intravenous efficacy through protection against complement-mediated lysis and evasion of neutralization by vaccinia virus... by Dong-Sup Lee, Mi-Ju Park, Namhee Lee, Yun-Hui Jeon, Jiyoon Yoo, Suk-kyung Shin, Songyi Lee, Byung-Jin Jung, Yun-Kyoung Hong, Keunhee Oh

“…The SJ-600 series viruses also expressed human granulocyte-macrophage colony-stimulating factor (GM-CSF) to activate dendritic cells. …”
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Construction and Pathogenicity Analysis of a GFPuv-labeled Peanut Ralstonia solanacearum Strain by Jinling HUANG, Yushuang WU, Huiquan TANG, Minyi HUANG, Ruixue YANG, Qiang WANG, Yong YANG, Xiaodan TAN

“…In liquid medium, the OD600 value of PeaFJ1-GFPuv strain exceeded 1.5 after 96 hours; in solid medium, the OD600 value exceeded 0.5 after 48 hours, with colony diameters of 1.0-1.5 mm within three days, showing no significant difference in growth rate compared with that of the wild-type strain. …”
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Single-cell profiling of SLC family transporters: uncovering the role of SLC7A1 in osteosarcoma by Yan Liao, Junkai Chen, Hao Yao, Ting Zheng, Jian Tu, Weidong Chen, ZeHao Guo, Yutong Zou, Lili Wen, Xianbiao Xie

“…CCK8 assays, EdU staining, colony formation assays, Transwell assays, and co-culture systems were used to assess the effects of SLC7A1 on cell proliferation, metastasis, and macrophage polarization. …”
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Klebsiella pneumoniae induces dose-dependent shock, organ dysfunction, and coagulopathy in a nonhuman primate critical care model by Jeffrey R. Strich, Marcos J. Ramos-Benitez, Seth Warner, Heather Kendall, Sydney Stein, Andrew P. Platt, Sabrina C. Ramelli, Shelly J. Curran, Izabella Lach, Kiana Allen, Ashley Babyak, Luis J. Perez-Valencia, Mahnaz Minai, Junfeng Sun, Kevin M. Vannella, Derron Alves, Richard Herbert, Daniel S. Chertow

“…Neutrophil activation evidenced by increased CD11b expression, decreased CD62L expression, and increased circulating levels of myeloperoxidase, lactoferrin, and neutrophil extracellular traps; monocyte activation evidenced by increased circulating levels of interleukin-6, tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor, and monocyte chemotactic protein-1; and endothelial activation evidenced by increased circulating levels of syndecan-1 and angiopoietin-II were all consistent with human sepsis. …”
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Brucea javanica oil inhibited the proliferation, migration, and invasion of oral squamous carcinoma by regulated the MTFR2 pathway by Yihan Lai, Yihan Lai, Mingkang Li, Mingkang Li, Juan Zhan, Juan Zhan, Lin Jiang, Lin Jiang, Yuan Wu, Zhiyi Fang, Jianhan Zhou, Jianhan Zhou, Yujie Ma, Yisen Shao, Yisen Shao, Wei Wang, Wei Wang

“…Studies have shown that Brucea javanica oil (BJO) extracts have anti-cancer effects against a variety of cancers, but little research has been reported on OSCC. MethodsCCK8, Colony formation, Scratch test and Transwell invasion assays were applied to determine the effects of BJO on the proliferation, migration, and invasion ability of OSCC cells in vitro. …”
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Identification and validation of BATF as a prognostic biomarker and regulator of immune cell infiltration in acute myeloid leukemia by Zhe Zhao, Dongmei Wang, Dongmei Wang, Xue Sheng, Shuying Li, Tingting Liu, Mengyuan Chang, Lei Feng, Di Zhang, Chunyan Ji, Chunyan Ji, Fei Lu, Jingjing Ye

“…In the meantime, the prognostic value of BATF was evaluated using univariate and multivariate analyses, receiver operating characteristic (ROC) curve (AUC) analysis, and Kaplan-Meier (KM) survival analysis. EdU, colony formation, and CCK-8 assays were employed to evaluate the proliferation of cells. …”
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ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma by Yong Zhuang, Qingyan Cai, Xin Hu, Huibin Huang

“…Furthermore, cell viability was assessed using the CCK-8 assay, while the impact on cell proliferation was examined using plate colony formation assay. Cell invasion was evaluated using the Transwell assay. …”
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Le mouvement étudiant et la question des langues en Algérie (1962-1965) : à propos d’un épisode méconnu de l’histoire de l’UGEMA-UNEA by Yassine Temlali

“…In Algeria in 1963, a year after the disappearance of the chair of Berber in the wreck of the colonial university, there was a brief flourishing of student demands for the “development of the Kabyle language” and the “creation of an institute for the teaching of Berber”. …”
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Growth performance and gut health of Murrah buffalo calves fed with developed novel synbi- otic formulation by Antony J. Thanippilly, Sachin Kumar, Vinay V. Varada, Nitin Tyagi, Ashish K. Samanta

“…Microbiota enumeration of health-promoting (lactobacilli and bifidobacterial) and health-negative (coliform and clostridium) was enumerated by colony-forming method to assess gut health. Results indicated that inulin showed the highest (p<0.05) prebiotic index and prebiotic activity score by FOS, MOS, and dextrins; hence, inulin was chosen for synbiotic formulation with L. salivarius BF17. …”
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Dimorphisme sexuel dans la force de morsure et de préhension chez le microcèbe mignon et héritabilité des caractères by Pauline Thomas, Anthony Herrel, Emmanuelle Pouydebat, Fabienne Aujard

“…Afin d’étudier les conséquences du dimorphisme morphologique chez les prosimiens, nous avons étudié une colonie de microcèbes captifs en mesurant les dimensions de la tête et des membres et en les pesant. …”
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Comparison of Nasal Colonization of Methicillin-Resistant Staphylococcus aureus in HIV-Infected and Non-HIV Patients Attending the National Public Health Laboratory of Central Nepa... by Kalash Neupane, Binod Rayamajhee, Jyoti Acharya, Nisha Rijal, Dipendra Shrestha, Binod G C, Mahesh Raj Pant, Pradeep Kumar Shah

“…The isolates were identified on the basis of colony characteristics and a series of biochemical tests. …”
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Effects of Bedaquiline on Antimicrobial Activity and Cytokine Secretion of Macrophages Infected with Multidrug-Resistant Mycobacterium tuberculosis Strains by Xia-Li Lyu, Ting-Ting Lin, Jing-Tao Gao, Hong-Yan Jia, Chuan-Zhi Zhu, Zi-Hui Li, Jing Dong, Qi Sun, Wei Shu, Sai-Sai Wang, Li-Ping Pan, Hai-Rong Huang, Zong-De Zhang, Qi Li

“…Macrophages infected with MDR-MTBs or H37Rv received Bdq treatments (4 h/8 h/24 h/48 h) at 1 × the minimum inhibitory concentration (1 × MIC), 10 × MIC and 20 × MIC. Intracellular colony-forming units (CFUs) and culture supernatant IL-12/23 p40, TNF-α, IL-6, and IL-10 were determined using the Luminex® 200TM system. …”
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Correlation analysis of DLG5 and PD-L1 expression in triple-negative breast cancer by Jingmin Che, Bo Chen, Xusheng Wang, Baoe Liu, Cuixiang Xu, Huxia Wang, Jingying Sun, Qing Feng, Xiangrong Zhao, Zhangjun Song

“…A combination of PD-L1 inhibitors and DLG5 RNA interference techniques was used, along with various methods including cell counting kit-8 (CCK-8), colony formation, wound healing, transwell migration, reverse transcription-quantitative real-time PCR (RT-qPCR), immunofluorescence, immunohistochemical staining (IHC), expression analysis from datasets and western blotting. …”
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Characterization of Listeria monocytogenes strains isolated from soil under organic carrot farming by Maciej Nowak, Zbigniew Paluszak, Natalia Wiktorczyk-Kapischke, Katarzyna Grudlewska-Buda, Anna Budzyńska, Joanna Skonieczna-Kurpiel, Ewa Wałecka-Zacharska, Monika Huse-Kutowska, Krzysztof Skowron

“…The number of biofilm-isolated rods from the stainless steel surface was 6.37 to 7.10 log colony-forming unit (CFU)/cm2, while on polypropylene it was from 6.75 to 8.06 log CFU/cm2. …”
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EGFR inhibition augments the therapeutic efficacy of the NAT10 inhibitor Remodelin in Colorectal cancer by Yongbin Zheng, Dan Song, Ming Guo, Chenhong Wang, Mingzhen Ma, Gongcai Tao, Licui Liu, Xiaobo He, Fengyu Cao, Dan Luo, Qingchuan Zhao, Zhongyuan Xia, Yanxin An

“…Additional in vivo and in vitro experiments, including CCK-8 assays, colony formation and mouse xenograft models, were conducted to explore the biological role of NAT10-mediated ac4C modifications. …”
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