Identification of cancer cell-intrinsic biomarkers associated with tumor progression and characterization of SFTA3 as a tumor suppressor in lung adenocarcinomas by Yu Zhao, Chengcheng Zhou, Ling Zuo, Haoming Yan, Yuhan Gu, Hong Liu, Guiping Yu, Xiaorong Zhou

“…Furthermore, a series of biological experiments, including cell viability assays, scratch wound healing assays, and colony formation assays, were conducted to demonstrate the tumor-suppressive effects of SFTA3. …”
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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…Viability findings were compared with colony-forming units (CFU/mL), a standard microbial viability measure.ResultsCAM/TMA-DPH staining demonstrated strong positive correlations with CFU counts across all bacterial species (r = 0.59 - 0.91), accurately reflecting biofilm vitality. …”
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Prevalence and determinants of nasal carriage of penicillin non-susceptible Streptococcus pneumoniae: a cross-sectional household survey in northern VietnamResearch in context by Costanza Tacoli, Hien Anh Thi Nguyen, Tu Cam Thi Nguyen, Bich Ngoc Thi Vu, Max van Wijk, Quynh Dieu Pham, Huong Kieu Thi Tran, Thuong Hong Thi Nguyen, Trang Thu Nguyen, Tung Son Trinh, Dung Tien Viet Vu, Hoang Huy Tran, Thai Duy Pham, Duc Anh Dang, Tien Dac Tran, Duong Thanh Nguyen, H. Rogier van Doorn, Thomas Kesteman, Sonia Lewycka

“…A total of 1, 498 samples from nasal and nasopharyngeal swabs were cultured on blood agar plates supplemented with 5 μg of gentamicin. S. pneumoniae colonies were confirmed by MALDI-TOF. Penicillin susceptibility was tested by E-test. …”
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Post-vaccination campaign evaluation of systemic and mucosal immunity of trivalent oral poliovirus vaccine in Karachi, Pakistan (2020–2021): a cross-sectional studyResearch in cont... by Ali Faisal Saleem, Visalakshi Jeyaseelan, Zaubina Kazi, Mahjabeen Zehra, Muhammad Masroor Alam, Grace Macklin, Rocio Lopez Cavestany, Sajid Muhammad, Najeeb Rehman, Ondrej Mach

“…Methods: A cross-sectional survey was conducted in four peri-urban sites (Cattle Colony, Ibrahim Hyderi, Ali Akber Shah, Rehri Goth) Karachi, Pakistan. …”
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Modulating tumor-associated macrophages through CSF1R inhibition: a potential therapeutic strategy for HNSCC by Kaiting Chen, Xiaochen Li, Shuyi Dong, Yu Guo, Ziyin Luo, Shi-Min Zhuang, Jie Liu, Tianrun Liu, Jing Liao, Weiping Wen

“…Flow cytometry was utilized to measure the expression of key macrophage markers and assess apoptosis following treatment with colony stimulating factor 1 receptor (CSF1R) inhibitors (BLZ945, PLX3397). …”
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Eicosapentaenoic Acid and Docosahexaenoic Acid as an Antimicrobial Agent in Orthopedics—An In Vitro Study About the Race for Surface by Christopher Spiegel, Burak Ünalan, Andreas Kaserbacher, Rohit Arora, Débora C. Coraça-Huber

“…After establishing the co-culture, cell culture and biofilm, we performed colony-forming unit counting and cell counting to examine cell survivability. …”
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Kaempferol and Vitamin E Improve Production Performance by Linking the Gut–Uterus Axis Through the Reproductive Hormones and Microbiota of Late-Laying Hens by Jing Zhang, Jie Zhang, Kangle Li, Xinyue Fu, Yanhui Liang, Minling Zhang, Shaolong Zhuang, Yuyun Gao

“…In the uterus, the KAE group and the KAE + VE group’s <i>ESR1</i> gene expression levels were dramatically higher, and the KAE + VE group’s <i>ESR2</i> and <i>FSHR</i> gene expression levels were significantly higher. 16S rRNA gene sequencing revealed a significant aggregation of cecum and uterus colonies in the Beta diversity PCoA. In the cecum, Firmicutes, Bacteroidetes, and WPS-2 were the dominant phylums. …”
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Vertical Transmission of Group B Streptococcus, Prevalence, Associated Factors, and Antimicrobial Susceptibility Profile among Newborns Delivered at Health Facilities in Jigjiga Ci... by Addisu Tesfaye, Mahlet Fekede, Fasil Getu, Surafel Mekuria, Tigist Abebe, Daniel Tollosa, Shamil Barsenga, Bawlah Tahir, Abdurahman Kedir Roble, Sara Tesfaye, Muluken Walle

“…Samples were inoculated on Todd Hewitt broth media supplemented with gentamycin and nalidixic acid and then subcultured on blood agar. Colony characteristics, Gram stain, and catalase test were used for identification. …”
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Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing by Wen-Shuo Chang, Chien-Cheng Huang, Tzu-Hao Chen, Ssu-Han Chao, Cheng-Hsien Lin, Ching-Ping Chang, Chi-Sheng Chien

“…Results: hPRP showed significantly higher concentrations of growth factors such as activin-A, brain-derived neurotrophic factor, nerve growth factor, Flt-3 Ligand, granulocyte-macrophage colony-stimulating factor, hepatocyte growth factor, and platelet-derived growth factor, compared to nPRP. …”
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Mechanism of Bone-metastatic LUAD Cells Promoting Angiogenesis 
Through HGF/YAP Signaling Pathway by Yan DENG, Rong QIU, Xingyu LIU, Yingyang SU, Yang XUE, Yuzhen DU

“…Methods The culture media from bone-metastatic LUAD cell A549-GFP-LUC-BM3 (BM3-CM) and A549-GFP-LUC (A549-CM) were separately applied to human umbilical vein endothelial cell (HUVEC). A colony formation assay, scratch assay, and tube formation assay were conducted to evaluate the proliferation, migration, and angiogenesis of HUVEC. …”
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Aspergillus fumigatus Influences Gasdermin-D-Dependent Pyroptosis of the Lung via Regulating Toll-Like Receptor 2-Mediated Regulatory T Cell Differentiation by Wei Yan, Yi-si Zhao, Ke Xie, Yu Xing, Fang Xu

“…The fungal load in the lung tissue was determined by the plate colony count. ELISA kit was used to detect the lung tissue homogenate proinflammatory cytokines TNF-α, IL-6, CCL2, and VEGF. …”
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Lactate Dehydrogenase-A-Forming LDH5 Promotes Breast Cancer Progression by Fang T, Hu L, Chen T, Li F, Yang L, Liang B, Wang W, Zeng F

“…The in vitro proliferation, migration and invasion potentials of BC cells were evaluated using MTT assays, colony formation, wound-healing assay, matrix metalloproteinase assays and transwell assays, respectively. …”
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PLL-g-HPA Hydrogel Loaded Human Umbilical Cord Mesenchymal Stem Cells Promote Burn Wound Healing in Rat Model by Regulating Inflammation Response by Tian L, Wang Z, Lei T, Feng L, Li Y, Wang K, Zhang Y, Zhang C, Liu J, Xing H, Ren W

“…The cytotoxicity to human foreskin fibroblasts (HFF) were assessed by CCK-8 assay and live/dead quantification and antibacterial activity against Escherichia coli and Staphylococcus aureus was also detected by colony forming unit. A full-thickness burn wound injury model in 12 SD rats was established, and the therapeutic effect of PLL-g-HPA hydrogel combined with hUC-MSCs was detected by healing time/Histology/inflammation factor expression level.Results: The findings from SEM, FTIR, and HFF analyses demonstrated the successful synthesis of PLL-g-HPA hydrogels. …”
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Traitement d’un lymphome agressif chez un babouin naturellement infecté par STLV-1 by Jocelyn Turpin, Sandrine Alais, Ambroise Marçais, Julie Bruneau, Anat Melamed, Nicolas Gadot, Olivier Hermine, Charles Bangham, Romain Lacoste, Renaud Mahieux

“…Au cours du contrôle sanitaire annuel dans notre colonie de Papio anubis naturellement infectés par STLV-1 (n=45), une femelle de 9 ans fut identifiée comme présentant des troubles respiratoires, un amaigrissement prononcé, un nombre de lymphocytes supérieur à 1010/L, des métastases pulmonaires et des lésions de la peau, similaires à celles qui sont observées chez les patients souffrant d’ATLL. …”
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A Multifunctional MIL-101-NH2(Fe) Nanoplatform for Synergistic Melanoma Therapy by Shang J, Chen Y, Wang F, Yang J, Li Y, Yang L, Liu X, Zhong Z, Yue C, Zhou M

“…Cellular studies using B16F10 cells assessed cytotoxicity, cellular uptake, migration inhibition, and colony formation suppression. In vivo experiments in melanoma-bearing mice evaluated antitumor efficacy and systemic safety through tumor growth inhibition, histological analyses, and toxicity assessments.Results: MIL@DOX@ICG exhibited a uniform octahedral structure with a particle size of approximately 139 nm and high drug loading efficiencies for DOX (33.70%) and ICG (30.59%). …”
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Menaces pesant sur une population de Galagos nains, Galagoides demidoff phasma (Galagonidae, Primates), dans un quartier périphérique de la ville de Kinshasa à N’Djili-Brasseries e... by Nathalie Mvudi Nango, Jean Mukulire Malekani, Lem ’ s Kalemba

“…Il s’agit d’un site agricole créé en 1954 par l’Etat colonial en vue de contribuer à la sécurité alimentaire dans la ville de Kinshasa et d’une succession de domaines privés ou publics où se réalisent divers types d’activités. …”
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Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production by Guangqiang Wei, Daodian Wang, Daodian Wang, Teng Wang, Gao Wang, Yunmei Chai, Yufang Li, Minhui Mei, Hao Wang, Aixiang Huang

“…In this study, whole genome sequencing analysis and corresponding in vitro assays were performed to investigate the probiotic potential and safety properties of L. fermentum A51.ResultsScanning electron microscopy (SEM) observed that L. fermentum strain A51 adhered into clusters and its colony exhibited the obvious silk drawing phenomenon. …”
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Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer by Hong Liu, Jing Wang, Yunhai Li, Feng Luo, Lei Xing

“…BC cell proliferation was detected with the Cell Counting Kit-8 and by the colony formation assay. Cell cycle progression was analyzed by flow cytometry, and the expression of cell cycle-related proteins (E2F1, cyclin D3, and cyclin-dependent kinase 2) was determined by western blots in SAE1 small interfering RNA (siRNA) transfected cells. …”
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Physicochemical characterization of biodegradable polymer polyhydroxybutyrate from halophilic bacterium local strain Halomonas elongata by W.O.S. Rizki, E. Ratnaningsih, D.G.T. Andini, S. Komariah, A.T. Simbara, R. Hertadi

“…Thermogravimetric analysis, differential thermogravimetry, and differential thermal analysis were used to analyze thermal stability.FINDINGS: Halomonas elongata produced polyhydroxybutyrate utilizing glucose as a carbon source, as evidenced by orange-fluorescence colonies under ultraviolet light. The optimum condition of polyhydroxybutyrate production was achieved when the bacterium was cultivated in a high medium containing 5 percent sodium chloride, 0.2 percent yeast extract, and 5 percent glucose (as measured by weight per volume) after 72 hours of incubation. …”
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Amoeba plate test with Acanthamoeba castellanii as an innovative tool for Nocardia recovery from sputum samples: a proof-of-concept study by Coralie Espinasse, Ghislaine Descours, Marine Ibranosyan, Laetitia Beraud, Christophe Ginevra, Joelle Chastang, Oana Dumitrescu, Frédéric Laurent, Verónica Rodriguez Nava, Sophie Jarraud, Camille Allam

“…The present findings are strong arguments to implement APT as a complementary technique for Nocardia isolation from heavily contaminated samples.IMPORTANCEThe culture-proven diagnosis of Nocardiosis is challenging due to the difficulties in recovering Nocardia colonies from respiratory samples containing a complex polymicrobial flora. …”
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