Attenuation of acute kidney injury in a murine model of neonatal Escherichia coli sepsis by Esther M. Speer, Atilade A. Adedeji, Joyce Lin, Alexandra Khorasanchi, Asma Rasheed, Maya Bhat, Kelly Mackenzie, Randolph Hennigar, Kimberly J. Reidy, Robert P. Woroniecki

“…We hypothesized that addition of PTX to antibiotics may attenuate immune and histological AKI in a murine neonatal sepsis model.MethodsPostnatal (PN) day 1 C57BL/6J mice were injected with E. coli K1 strain at 105 colony forming units per gram weight or saline control. …”
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Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy by Gregory B. Lesinski, Chrystal M. Paulos, Maggie Phillips, Jacklyn Hammons, Isaac Karpovsky, Zaid Mahdi, Natalie K. Horvat, Megan M. Wyatt, Margaret A. Hall, Cameron J. Herting, Richard A. Moffitt

“…However, these models have limitations, including marked tumor variability across individual mice and the cost of colony maintenance.Methods Using flow cytometry and immunohistochemistry, we characterized the immunological features and trafficking patterns of adoptively transferred T cells in orthotopic PDAC (C57BL/6) models using two mouse cell lines, KPC-Luc and MT-5, isolated from C57BL/6 KPC-GEMM (KrasLSL-G12D/+p53−/− and KrasLSL-G12D/+p53LSL-R172H/+, respectively).Results The MT-5 orthotopic model best recapitulates the cellular and stromal features of the TME in the PDAC GEMM. …”
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Immunogenicity in humans of a transdermal multipeptide melanoma vaccine administered with or without a TLR7 agonist by Craig L Slingluff, Gina R Petroni, Walter C Olson, Kimberly A Chianese-bullock, Donna Deacon, Mark Smolkin, William W Grosh, Max O Meneveau, Kevin T Lynch, Elise P Salerno, Elizabeth Woodson, James W Patterson

“…We hypothesized that: (1) applying melanoma-derived peptides topically, in proximity to LC, could be immunogenic and safe, with low vaccine-site toxicity and (2) topical toll-like receptor 7 (TLR7) agonist would increase immunogenicity of the peptide vaccine.Methods Twelve melanoma peptides plus a tetanus helper peptide were combined with granulocyte macrophage colony stimulating factor (GM-CSF) and were administered topically on days 1, 8, and 15, to 28 patients randomized to one of four adjuvant preparations: (1) incomplete Freund’s adjuvant (IFA); (2) IFA plus a TLR7 agonist (imiquimod) administered on days 0, 7, 14; (3) dimethyl sulfoxide (DMSO) or (4) DMSO+ imiquimod administered on day 0, 7, 14. …”
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Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 by Hanie Pooshideh, Jalal Jafarzade, Ali Heidarpour, Mojtaba Taghizade Armaki, saeid Mahdavi Omran, Firoozeh Kermani, Mohsen Karami

“…The different yeast and filamentous colonies were distinguished using standard mycological methods. …”
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Rescue of in vitro models of CSF1R-related adult-onset leukodystrophy by iluzanebart: mechanisms and therapeutic implications of TREM2 agonism by Kelley C. Larson, Frederick W. Gergits, Abigail J. Renoux, Elizabeth J. Weisman, Borislav Dejanovic, Liyue Huang, Bhaumik Pandya, Donald G. McLaren, Berkley A. Lynch, Richard Fisher, Evan Thackaberry, David Gray, Francois Gaudreault, Christian Mirescu

“…CSF1R-ALSP is caused by heterozygous loss-of-function mutations in the colony stimulating factor 1 receptor (CSF1R) gene, which encodes a receptor required for the differentiation of myeloid cells, as well as for microglial survival and proliferation. …”
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Clinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma by Liuyan Chen, Lvying Wu, Minying Tang, Yuanhang Cheng, Kuanyin Wang, Jianan Zhang, Wenyi Deng, Lingfeng Zhu, Jin Chen

“…Various assays, including assessments of cell proliferation, apoptosis, the cell cycle, cell migration and invasion, and colony formation, and xenograft tumor models were subsequently performed following to the knockdown of DBF4 expression via shRNA. …”
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Reproducibility and Consistency of Isolation Protocols for Fibroblasts, Smooth Muscle Cells, and Epithelial Cells from the Human Vagina by Jayson Sueters, Rogier Schipperheijn, Judith Huirne, Theo Smit, Zeliha Guler

“…The best results were obtained after digestion with 0.1% collagenase-IV, where pure FB colonies formed with high cell viability. (4) Conclusions: Today, vaginoplasty is considered the gold standard for surgically creating a neovagina, despite its considerable drawbacks and limitations. …”
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CXCR4 promotes tumor stemness maintenance and CDK4/6 inhibitors resistance in ER-positive breast cancer by Qiangfeng Shi, Wang Yang, Yiye Ouyang, Yujie Liu, Zijie Cai

“…The effects of CXCR4 knockdown and overexpression via siRNAs and plasmids transfection were examined by mammosphere formation, RT-qPCR, flow cytometry, MTT and colony formation assays. The regulation mechanisms were analyzed by RT-qPCR, western blotting and immunofluorescence experiments. …”
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The ground beneath our feet: A critical reflection on 135 years of landscape evolution models for southern Africa by Rivoningo Khosa, Vela Mbele, Kelly Kirsten, Robyn Pickering

“…While the evidence of a homogeneous and stable landscape is continually being challenged through scientific advancement, this terminology is rooted in outdated colonial thinking. We also note that the key narratives that have driven research on landscape evolution have been largely shaped by selected prominent Western-based scientists. …”
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Migration, race, and gender: the policing of subversive solidarity actors in Morocco by Sébastien Bachelet, Maria Hagan

“…This is reinforced by the persistence of racial hierarchies of domination in post-colonial Morocco, which still bear the legacy of the active enslavement of Black people in the country and the legacy of the country’s colonisation by Europeans. …”
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The effect of smoking on the immediate effectiveness of induction chemotherapy in the combined treatment of HPV-associated oropharyngeal cancer by P. V. Golubev, L. V. Bolotina, A. R. Gevorkov, T. I. Deshkina

“…Patients in both groups received prophylactic granulocyte colony-stimulating factor. At the second stage, standard chemoradiotherapy was carried out against the background of drug radiomodification with carboplatin. …”
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Inhibitory potential of probiotic spore formers grown as mono- and mix-cultures under conditions of different nutrient availability by O. V. Knysh, A. V. Martynov, T. P. Osolodchenko

“…Co-cultivation and cultivation on semi-starved media were accompanied by an increase in the number of isolated colonies of probiotic bacilli (disseminates) in the transparent zone of no growth and the pathobiont growth zone. …”
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Contezolid Harbored Equivalent Efficacy to Linezolid in Tuberculosis Treatment in a Prospective and Randomized Early Bactericidal Activity Study by Jiang G, Liu R, Xue Y, Ge Q, Nie L, Lv Z, Kong Z, Shi J, Chen H, Li H, Wu X, Xie L, Song Y, Huang H, Gao M

“…Each sample was processed for the enumeration of acid-fast bacilli (AFB) colonies, and time-to-positivity (TTP) during MGIT960 liquid culture was recorded.Results: A total of 10 eligible patients were enrolled in each treatment group, although one patient in the CZD group was later excluded from the analysis. …”
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Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption by Yi Zhang, Yongding He, Meixin Yan, Shilong Zhang, Sisi Zhou, Wankuan Shen

“…On YePSA medium, the gene SsMFSST1 does not affect the growth, spore morphology, colony morphology, and oxidative stress ability of the haploid spores of S. scitamineum. …”
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Protective factors, risk factors, and intervention strategies in the prevention and reduction of crime among adolescents and young adults aged 12-24 years: A scoping review protoco... by Rosanna Mary Rooney, Amber Hopkins, Jacob Peckover, Kael Coleman, Rebecca Sampson, Rosa Alati, Sharinaz Hassan, Christina M Pollard, Jaya A R Dantas, Roanna Lobo, Zakia Jeemi, Sharyn Burns, Robert Cunningham, Stephen Monterosso, Lynne Millar, Sender Dovchin, Rhonda Oliver, Ranila Bhoyroo, Getinet Ayano

“…This is a critical gap given the disproportionally high rates of incarceration and youth detention among First Nations people globally, particularly in countries with a colonial past. The aim of this scoping review is to identify and map the key risk and protective factors, along with intervention strategies, that are essential for recognizing adolescents and young adults at risk of crime.…”
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RBM47 promotes cell proliferation and immune evasion by upregulating PDIA6: a novel mechanism of pancreatic cancer progression by Yihui Ma, Enjie Liu, Huijie Fan, Chenfei Li, Pei Huang, Meiying Cui, Zhengyang Wang, Jing Zhou, Kuisheng Chen

“…Loss- and gain-of-function effects of RBM47 on cell proliferation, tumor growth, and immune evasion were determined by CCK-8, EdU incorporation, colony formation assays, the xenogeneic tumor model, and co-culture system of PC and NK-92 cells. …”
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Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig by Kunhong Xie, Weidong Cai, Lingjie Li, Bing Yu, Yuheng Luo, Zhiqing Huang, Xiangbing Mao, Jie Yu, Ping Zheng, Hui Yan, Hua Li, Jun He

“…From 1 to 15 d, the CA and DA groups were orally fed with 1.0 × 1011 colony-forming units A. muciniphila per day, while the CON and DCON groups were received gastric infusion of anaerobic sterile saline per day. …”
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Circular RNA RHOT1 Regulates miR-142-5p/CCND1 to Participate in Chondrocyte Autophagy and Proliferation in Osteoarthritis by Gu Man, Haiwen Yang, Kai Shen, Donghong Zhang, Julin Zhang, Hao Wu, Hongyu Zhang, Jinhuan Wang

“…The function of circRHOT1 in the regulation of OA was assessed by CCK-8, colony formation, flow cytometry analysis, quantitative real-time PCR, Western blot analysis, and luciferase reporter gene assay in chondrocytes. …”
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The effect of GM-CSF and predictors of treatment outcome in pediatric septic shock patients by Zhen-Hao Yu, Gui-Xiang Tian, Yao-Dong Wang, Ting-Yan Liu, Peng Shi, Jia-Yun Ying, Wei-Ming Chen, Yu-Feng Zhou, Guo-Ping Lu, Cai-Yan Zhang

“…Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been explored as a therapeutic intervention to counteract this immunosuppression. …”
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MSCs with upregulated lipid metabolism block hematopoietic stem cell differentiation via exosomal CTP-1A in MDS by Chunlai Yin, Xue Yan, Jinyi Ren, Cheng Zhang, Jiaqing Liu, Zilong Wang, Jing Liu, Weiping Li, Xia Li

“…The proliferation, apoptosis, differentiation and support for hematopoiesis of these cells were analyzed using CCK-8 assay, FC and induction medium and CFU (colony forming units) assay, respectively. Expression levels of metabolic molecules were used as indicators to screen MSCs with different metabolic pathways and were detected by RT-PCR and Western blotting. …”
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