Distribution of Aeromonas bacterial population in water, sediment and Nile tilapia in fish culture pond, Guder, Ethiopia by Bekele Lema, Natarajan Pavanasam, Kassaye Balkew, Zufan Bedewi

“…The level of bacterial population in water, sediment and fish tissue were done by following standard methods and expressed as colony-forming units (CFU) in water per milliliter (CFU ml-1), sediment and fish tissues(CFU g-1).The results revealed more bacterial population in sediment (3.43 x106 to 5.54 x 106 CFU g-1) than in water (1.45x106 to 4.0x106 CFU ml-1) and  fish tissues (1.06 ± 1.10x104 to 1.74 ± 10.8x104 CFU g-1in gill filaments; 1.62 ± 11.2x104 to 2.82 ± 13.0x104 CFU g−1 in intestine from; and 0.82 ± 5.9x104 to 1.60 ± 12.1.6x104 CFU g-1; in kidney from 0.48 ± 5.0 to 0.77 ± 4.1x104 CFU g-1. in skin). …”
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Evaluation of the effect of Irritrol as final irrigant against Staphylococcus epidermidis: an in Vitro Study. by Hezha Saadi Hassan, Diyar Khalid Bakr

“…Results: In decreasing the amount of bacteria colonies G4 (Irritol with activation) 94% and G5 (NaOCl 5.25%), 92% of the CFU were decreased compared to the control group. …”
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lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis by YiFan Wang, GuanYin Zhu, Fang Pei, ZhiHe Zhao

“…The expression of SNHG15 was increased in THP-1 cells stimulated by macrophage colony-stimulating factor (M-CSF)/receptor activator of nuclear factor-kappa B ligand (RANKL). …”
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DLAT is involved in ovarian cancer progression by modulating lipid metabolism through the JAK2/STAT5A/SREBP1 signaling pathway by Hui Wang, Shen Luo, Yue Yin, Yang Liu, Xiaomei Sun, Ling Qiu, Xin Wu

“…The effects of DLAT silencing on changes in lipid metabolism, cell viability, migration, and invasion were examined in SKOV3 and OVCAR3 cells using CCK-8, colony formation, Transwell migration and invasion, and wound-healing assays. …”
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Antimicrobial Resistance Patterns of Bacterial Isolates from Blood Culture among HIV/AIDS Patients at Felege Hiwot Referral Hospital, Northwest Ethiopia by Mohabaw Jemal, Teshiwal Deress, Teshome Belachew, Yesuf Adem

“…Identification was performed using colony characteristics and standard biochemical techniques. …”
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Unveiling the Potency of Gardenia Extract Against <i>H. pylori</i>: Insights from In Vitro and In Vivo Studies by Pornpen Werawatganone, Duangporn Werawatganon, Nattida Noonak, Maneerat Chayanupatkul, Tanittha Chatsuwan, Naruemon Klaikeaw, Walaisiri Muangsiri, Prasong Siriviriyakul

“…Thirty-five male Sprague–Dawley rats were inoculated with <i>H. pylori</i> (10⁸–10¹⁰ colony-forming unit/mL) and divided into five groups. …”
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Enhanced myofibroblast differentiation of eMSCs in intrauterine adhesions by Jun Song, Meiqi Li, Yuan Tao, Yumeng Li, Canrong Mai, Jingting Zhang, Lan Yao, Shaoquan Shi, Jianyong Xu

“…The isolated eMSCs from IUA and controls show similar functions from the perspectives of cell morphology, proliferation, colony formation, exosome secretion, positive ratio of eMSC markers and conventional MSC markers, tri-differentiation efficiency, the ability of suppressing lymphocyte proliferation, cell aging, and promoting vascular tube formation. …”
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MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells by Hou Lingli, Liu Qi, Zhao Ying, Yang Hongwei, Meng Qingying, Yu Fei

“…They consist of the cell proliferation assay, wound healing assay, transwell assay, colony formation assays and qRTPCR. Expression levels of target proteins were determined using Western blotting. …”
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Baicalein induces apoptosis by inhibiting the glutamine-mTOR metabolic pathway in lung cancer by Jingyang Li, Di Zhang, Shaohui Wang, Peng Yu, Jiayi Sun, Yi Zhang, Xianli Meng, Juan Li, Li Xiang

“…In vitro experiments were used to assess the efficacy of baicalein (for H1299: 12.5, 25, and 50 μM; for A549: 10, 20, and 40 μM) on lung cancer cell proliferation, colony formation, and apoptosis. Metabolomics analysis was performed using liquid chromatography-mass spectrometry. …”
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Effect of extracted phycocyanin by-products as a synbiotic supplement on the production performance and intestinal morphology of broilers by Kannikar Hamprakorn, Buaream Maneewan, Wantamas Jantasin, Mohd Nizam Lani, Tossapol Moonmanee, Julakorn Panatuk

“…Materials and Methods: A total of 240 one-day-old male Ross 308 broilers were randomly distributed among five dietary treatment groups; they received a diet supplemented with a synbiotic product (probiotic [Lactobacillus johnsonii] at least 1.0 × 108 colony-forming unit/mg with prebiotic [by-product of phycocyanin extraction]) at 0.000%, 0.025%, 0.050%, 0.075%, and 0.100%. …”
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Aurora B inhibition induces hyper-polyploidy and loss of long-term proliferative potential in RB and p53 defective cells by Shivam Vora, Saptarshi Chatterjee, Ariel Andrew, Ramyashree Prasanna Kumar, Martina Proctor, Zhen Zeng, Rituparna Bhatt, Deborah Nazareth, Madushan Fernando, Mathew J. K. Jones, Yaowu He, John D. Hooper, Nigel A. J. McMillan, Jelena Urosevic, Jamal Saeh, Jon Travers, Daniela Cimini, Jing Chen, Brian Gabrielli

“…However, when AURKB inhibitor was removed after 1 day and cells had failed a single cytokinesis to become tetraploid, they retained colony forming ability and long-term proliferative potential. …”
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A pan-cancer analysis: predictive role of ZNF32 in cancer prognosis and immunotherapy response by Minghan Li, Chang Su, Qianru Wang, Yuetong Chen, Di Jiang, Weijia Wang, Shunjin Chen, Xiangping Li, Ming Fu, Juan Lu

“…Overexpression of ZNF32 significantly enhanced proliferation and migration in FaDu and CAL27 cell lines, as demonstrated through CCK-8 assays, colony formation, flow cytometry, Transwell migration, and Boyden invasion assays. …”
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Achieving equiaxed and lamellar TiAl alloys via cold-cathode electron beam additive manufacturing with dual-wire synergistic control by Yilei Shi, Shuili Gong, Haiying Xu, Wei Chen, Zhuang Wang, Guang Yang, Xin Liu, Bojin Qi

“…Compared to the lamellar microstructure of Ti48Al alloy, the Ti48Al1Mo0.45Zr0.3Si alloy exhibits a dual-γ phase microstructure, consisting of fine lamellar colonies and equiaxed γ grains. This modification led to a 90% and 150% increase in elongation at room temperature and 650 °C, respectively. …”
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Cultivatable Bacteriota of Chronic Wound of Patients with Diabetic Foot Syndrome with Critical Limb Ischemia Based on Wound Biopsy in Peri-Revascularization Period by Syedah Sarah Hussaini, Fran Dominic Grubesa, Mateusz Gajda, Martyna Schönborn, Katarzyna Bogucka, Mikołaj Maga, Paweł Maga, Jadwiga Wójkowska-Mach

“…The control parameters of blood supply improved; TCPO2 was 38.5 mmHg, the ABI was 0.9, and the TBI was 0.3, with a reduction in the average WIfI score to 3.7 (mild), while total colony-forming units (CFUs) increased by 13.5%. No cases of reocclusion or restenosis were observed during the study; however, small amputations were performed in two patients (8.7%). …”
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Predictive factors of thrombosis during autologous stem cell transplantation in multiple myeloma: Experience from EHU Oran (Algeria) by Mohammed Amine BEKADJA, Brahim BENZINEB, Mansour BELKACEM, Hafida OUDJERIOUAT, Badra ENTASOLTAN, Soufi OSMANI, Kamila AMANI, Samira BOUCHAMA, Leila CHAREF, Mohamed BRAHIMI, Abdessamad ARABI, Rachir Amar BOUHASS, Nabil YAFOUR

“…Thepredictive factors studied are: age, co-morbidities, use of IMiDs, use of Granocyte colony stimualing factor in mobilization, the central venous catheter insertion site, cytapheresis count, CD34+ perfused, and finally deep venous thrombosis prophylaxis by a low molecular weight heparin .The statistical analysis focused on a univariate study, followed by a multivariate analysis using binary logistic regression. …”
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Immunogenicity and protective efficacy of a multi-antigenic adenovirus-based vaccine candidate against Mycobacterium tuberculosis by Jin-Seung Yun, Jin-Seung Yun, Eunkyung Shin, Young-Ran Lee, Jung-Ah Lee, Hyeokjin Lee, Jong-Seok Kim, Sung Jae Shin, Sang-Jun Ha, Sang-Won Lee, Dokeun Kim, Jung-Sik Yoo, Hye-Sook Jeong

“…In this study, we aimed to evaluate the efficacy of a multi-antigenic recombinant Ad5 vectored vaccine and determine the optimal immunization route for enhanced immune response against Mycobacterium tuberculosis.MethodsWe constructed a multi-antigenic recombinant Ad5 vectored vaccine expressing four antigens (Ag85B-ESAT6-MPT64-Rv2660c) of M. tuberculosis (rAd-TB4), immunized with rAd-TB4 (5 × 107 infectious virus units/mouse) twice at an interval of 4 weeks starting at 10 weeks after BCG priming, and evaluated its boosting efficacy in a BCG-primed mouse model, and determined the optimal immunization route.ResultsCompared with the BCG-only (2 × 105 colony forming units/mouse), subcutaneous injection of rAd-TB4 (1 × 107 infectious virus units/mL; two doses) elicited a T-cell response and cytokine production in lung lymphocytes and splenocytes. rAd-TB4 immunization significantly reduced bacterial loads and inflamed lung areas compared to BCG immunization (p &lt; 0.01) and protected against the H37Rv challenge performed at 17 weeks of BCG priming. …”
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Predictive factors of thrombosis during autologous stem cell transplantation in multiple myeloma: Experience from EHU Oran (Algeria) by Mohammed Amine BEKADJA, Brahim BENZINEB, Mansour BELKACEM, Hafida OUDJERIOUAT, Badra ENTASOLTAN, Soufi OSMANI, Kamila AMANI, Samira BOUCHAMA, Leila CHAREF, Mohamed BRAHIMI, Abdessamad ARABI, Rachir Amar BOUHASS, Nabil YAFOUR

“…Thepredictive factors studied are: age, co-morbidities, use of IMiDs, use of Granocyte colony stimualing factor in mobilization, the central venous catheter insertion site, cytapheresis count, CD34+ perfused, and finally deep venous thrombosis prophylaxis by a low molecular weight heparin .The statistical analysis focused on a univariate study, followed by a multivariate analysis using binary logistic regression. …”
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Simvastatin Posttreatment Controls Inflammation and Improves Bacterial Clearance in Experimental Sepsis by Flora Magno de Jesus Oliveira, Cassiano Felippe Gonçalves-de-Albuquerque, Isabel Matos Medeiros de Moraes, Patrícia Alves Reis, Vinicius Novaes Rocha, Patrícia Torres Bozza, Adriana Ribeiro Silva, Hugo Caire de Castro Faria Neto

“…Simvastatin diminished the number of bacterial colony forming units (CFU) and increased the production of nitric oxide production in the peritoneum. …”
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Analysis of Nucleotide Alterations in the E6 Genomic Region of Human Papillomavirus Types 6 and 11 in Condyloma Acuminatum Samples from Brazil by Marina Carrara Dias, Bruna Stuqui, Paola Jocelan Scarin Provazzi, Cíntia Bittar, Natália Maria Candido, Renata Prandini Adum de Matos, Rodolfo Miglioli Badial, Caroline Measso do Bonfim, Patricia Pereira dos Santos Melli, Silvana Maria Quintana, José Antônio Cordeiro, Paula Rahal, Marilia de Freitas Calmon

“…The products were ligated to a cloning vector and five colonies of each sample were sequenced to observe the nucleotide alterations. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

“…The fertilized eggs were obtained through hormonal induction (5 μg/kg LHRH-A3, 600 IU/kg hCG and 2 mg/kg DOM) and artificial insemination, and the tyrb (tyrosinase b) or csf1ra (colony-stimulating factor 1 receptor a) gRNA and Cas9 mRNA were microinjected into the fertilized eggs at the one-cell stage for targeted gene editing. …”
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