TOM40 as a prognostic oncogene for oral squamous cell carcinoma prognosis by Lifei Deng, Hong Ran, Dunhui Yang, Zhen Wang, Peng Zhao, Hengjie Huang, Yongjin Wu, Peng Zhang

“…The TCGA-OSCC cohort was divided into the TOM40 high and TOM40 low groups and the correlation between TOM40 expression level and the sensitivity to frequently used chemotherapeutic drugs was evaluated. CCK-8 and colony formation assays were applied to determine the cell growth. …”
Get full text

Engineered GM-CSF polarizes protumorigenic tumor-associated macrophages to an antitumorigenic phenotype and potently synergizes with IL-12 immunotherapy by Yue Wang, Kevin Chang, Aslan Mansurov, Jun Ishihara, Seounghun Kang, Trevin Kurtanich, Hye Rin Chun, Anna J Slezak, Lisa R Volpatti, Thomas Wang, Aaron T Alpar, Kirsten C Refvik, O Isabella Hansen, Gustavo J Borjas, Brendan T K Berg, Ha-Na Shim, Kevin T Hultgren, Suzana Gomes, Ani Solanki, Melody A Swartz, Jeffrey A Hubbell

“…Therefore, the rational design of immune modulators that can control the immune suppressive properties of TAMs and polarize them, as well as dendritic cells (DCs), toward a more proinflammatory phenotype is a principal objective in cancer immunotherapy.Methods Here, using a protein engineering approach to enhance cytokine residence in the tumor microenvironment, we examined combined stimulation of the myeloid compartment via tumor stroma-binding granulocyte–macrophage colony-stimulating factor (GM-CSF) to enhance responses in both DCs and T cells via stroma-binding interleukin-12 (IL-12). …”
Get full text

Tasquinimod suppresses tumor cell growth and bone resorption by targeting immunosuppressive myeloid cells and inhibiting c-MYC expression in multiple myeloma by Marie Törngren, Rong Fan, Eline Menu, Helena Eriksson, Karin Vanderkerken, Andrew Chantry, Hatice Satilmis, Niels Vandewalle, Emma Verheye, Philip Vlummens, Anke Maes, Catharina Muylaert, Elke De Bruyne, Holly Evans, Nathan De Beule, Dirk Hose, Ken Maes, Karine Breckpot, Kim De Veirman

“…The in vivo impact on immune cells (myeloid cell subsets, macrophages, dendritic cells), tumor load, survival and bone disease were elucidated using immunocompetent 5TMM models.Results Tasquinimod treatment significantly decreased myeloma cell proliferation and colony formation in vitro, associated with an inhibition of c-MYC and increased p27 expression. …”
Get full text

Cytokines and Water Distribution in Anorexia Nervosa by Hubertus Himmerich, Bethan Dalton, Olivia Patsalos, Ulrike Schmidt, Iain C. Campbell

“…Following blood collection, 27 cytokines and chemokines were quantified using multiplex ELISA-based technology: Eotaxin, Eotaxin-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon- (IFN-) γ, interleukin- (IL-) 1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, interferon γ-induced protein- (IP-) 10, macrophage inflammatory protein- (MIP-) 1α, MIP-1β, monocyte chemoattractant protein- (MCP-) 1, MCP-4, thymus and activation-regulated chemokine (TARC), TNF-α, and TNF-β. …”
Get full text

In vitro inhibition of Xanthomonas vasicola pv. musacearum, the causal agent of banana Xanthomonas Wilt, using medicinal plant extracts from North Kivu, Eastern Democratic Republic... by Franchement Mukeshambala, Angele Ibanda, Ludivine Lassois, Gakuru Semacumu, Dhed’a Djailo, Leon Nabahungu, Guy Blomme, Godefroid Monde

“…The bacterial samples were collected from infected field-grown banana plants in South Kivu and isolated on Yeast Extract Peptone Agar (YPGA). Pure Xvm colonies were used for identification via i) Polymerase Chain Reaction (PCR) with specific primers and, ii) greenhouse inoculation trials. …”
Get full text

USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression by Shaoxuan Cheng, Zhiyuan Qiu, Ziyi Zhang, Yuxuan Li, Yue Zhu, Yuxin Zhou, Yinghui Yang, Yaowen Zhang, Dian Yang, Yingqiu Zhang, Han Liu, Zhaoxia Dai, Shu-Lan Sun, Shuyan Liu

“…The effects of USP39 depletion on tumor progression were tested using doxycycline-inducible USP39 knockdown and rescue lung adenocarcinoma cells both in vitro and in vivo by performing MTT, colony formation, EdU staining, transwell and tumor xenograft model experiments. …”
Get full text

Evaluation of Yellow Fever Virus Infection in <i>Aedes aegypti</i> Mosquitoes from Pakistan with Distinct Knockdown Resistance Genotypes by Carlucio Rocha dos Santos, Caleb Guedes Miranda dos Santos, Dinair Couto-Lima, Bárbara Silva Souza, Rafi Ur Rahman, Marcos Dornelas Ribeiro, José Bento Pereira Lima, Ademir Jesus Martins

“…<i>aegypti</i> from a Pakistani colony were exposed to YFV strains (PR4408/2008 and ES504/2017) along with the Rockefeller strain. …”
Get full text

Pooled safety analysis of two phase 3 studies investigating trifluridine/tipiracil plus bevacizumab in patients with metastatic colorectal cancer by Julien Taieb, Marwan Fakih, Gabor Liposits, Gerald W. Prager, Eric Van Cutsem, Fortunato Ciardiello, Nadia Amellal, Elizabeth Calleja, Mei Liu, Lucas Roby, Josep Tabernero, Thierry André

“…Overall, granulocyte colony-stimulating factor was used in 30.6% of patients. …”
Get full text

A bioprocess engineering approach for the production of hydrocarbons and fatty acids from green microalga under high cobalt concentration as the feedstock of high-grade biofuels by Alok Patel, Chloe Rantzos, Eleni Krikigianni, Ulrika Rova, Paul Christakopoulos, Leonidas Matsakas

“…Abstract Botryococcus braunii, a colonial green microalga which is well-known for its capacity to synthesize hydrocarbons, has significant promise as a long-term source of feedstock for the generation of biofuels. …”
Get full text

Domestication of different varieties in the cheese-making fungus Geotrichum candidum by Bennetot, Bastien, Vernadet, Jean-Philippe, Perkins, Vincent, Hautefeuille, Sophie, Rodríguez de la Vega, Ricardo C., O’Donnell, Samuel, Snirc, Alodie, Grondin, Cécile, Lessard, Marie-Hélène, Peron, Anne-Claire, Labrie, Steve, Landaud, Sophie, Giraud, Tatiana, Ropars, Jeanne

“…Another of the cheese populations seemed to represent a more advanced stage of domestication, with stronger phenotypic differentiation from the wild clade, harboring much lower genetic diversity, and phenotypes more typical of cheese fungi, with denser and fluffier colonies and a greater ability of excluding cheese spoiler fungi. …”
Get full text

Evidence that the domesticated fungus Leucoagaricus gongylophorus recycles its cytoplasmic contents as nutritional rewards to feed its leafcutter ant farmers by Caio Ambrosio Leal-Dutra, Lok Man Yuen, Bruno Augusto Maciel Guedes, Marta Contreras-Serrano, Pedro Elias Marques, Jonathan Zvi Shik

“…Abstract Leafcutter ants farm a fungal cultivar (Leucoagaricus gongylophorus) that converts inedible vegetation into food that sustains colonies with up to millions of workers. Analogous to edible fruits of crops domesticated by humans, L. gongylophorus has evolved specialized nutritional rewards—swollen hyphal cells called gongylidia that package metabolites and are consumed by ant farmers. …”
Get full text

Culturas en movimiento: migración y difusión-dilución de las creencias en México by Daniel Delaunay

“…En cuanto a los protestantes evangelistas, no se encontraran fundamentos demoeconómicos en su aptitud migratoria, por lo que ésta sí puede calificarse como cultural; su inclinación migratoria les sirve para difundir la iglesia evangelista, en particular las regiones de tradición más colonial que se resisten más a la dilución del catolicismo. …”
Get full text

Políticas de identidad y gestión sexual de los cuerpos: un análisis de los discursos legislativos acerca de la gestión estatal del conflicto trans en Chile by Dariela Milen Cristi Donoso

“…Lo que resulta clave para la reconfiguración de una ciudadanía excluyente, en cuanto se institucionalizan normas socio-sexuales que definirían las nociones emergentes de la figura de ciudadano/a, evidenciando que se enmarca en las fronteras del no-ser a toda corporalidad que no se manifieste bajo los márgenes de inteligibilidad que impone la lógica científica-colonial y la estructura político-administrativa del país.…”
Get full text

RET Inhibitor SPP86 Triggers Apoptosis and Activates the DNA Damage Response Through the Suppression of Autophagy and the PI3K/AKT Signaling Pathway in Melanoma Cells by Zhang Y, Liu H, Wang K, Zheng J, Luan H, Xin M

“…Thus, this study aims to explore the effects of SPP86 on autophagy and to elucidate its association with cell proliferation, apoptosis, and DNA damage in melanoma cells.Methods: This study assessed the anti-tumor effects of SPP86 on cell viability, colony formation, apoptosis, and DNA damage in two melanoma cell lines, A375 and A2058. …”
Get full text

The XIAP inhibitor AZD5582 improves the treatment effect of microwave ablation on hepatocellular carcinoma by Wenhui Wang, Fuyuan Wu, Zhe Wu, Mengfan Zhang, Qiang Lu

“…We established an in vitro IMWA model by heating HCC cell lines and, at the same time, applied the XIAP inhibitor AZD5582 and verified the proliferation, migration, and pro-apoptotic ability of the XIAP inhibitor on tumor cells using CCK8, colony formation assay, cell scratch assay, and flow cytometry flow. …”
Get full text

The concept of natural genome reconstruction. Part 1. Basic provisions of the “natural genome reconstruction” concept. Changing the genome of hematopoietic stem cells using several... by L. A. Yakubov, O. S. Taranov, S. V. Sidorov, S. D. Nikonov, A. A. Ostanin, E. R. Chernykh, N. A. Kolchanov, S. S. Bogachev

“…Extracellular fragments delivered to the internal compartments of hematopoietic stem cells initiate terminal differentiation, colony formation, and proliferation of hematopoietic precursors. …”
Get full text

Generation and characterization of chicken monocyte-derived dendritic cells by Elie Ngantcha Tatchou, Elie Ngantcha Tatchou, Romane Milcamps, Romane Milcamps, Guillaume Oldenhove, Bénédicte Lambrecht, Fiona Ingrao

“…In chicken, DCs have mainly been derived from bone marrow cell cultures, with limited characterization from blood monocytes.MethodsThe present study takes advantage of newly available chicken immunological tools to further characterize chicken monocyte-derived dendritic cells (MoDCs), focusing on their phenotype, and functions, including antigen capture and T-cell stimulation, and response to live Newcastle disease virus (NDV) stimulation.ResultsAdherent chicken PBMCs were cultured with recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), for 5 days, leading to the upregulation of putative CD11c and MHCII, markers of DC differentiation. …”
Get full text

Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…Reading by electroporation. The resulting colonies were confirmed by visualizing bioluminescence using an in vivo imaging system and by testing their resistance to chloramphenicol. …”
Get full text

Nocardiosis in Renal Allograft Recipients by Chilaka Rajesh, Athul Thomas, Jeethu Joseph Eapen, Sabina Yusuf, Elenjickal Elias John, Anna T. Valson, Suceena Alexander, Vinoi George David, Joy Sarojini Michael, Santosh Varughese

“…Multiple initially obtained cultures may be negative because of the slow growth of the organism and variable colony morphology. Hence, infected specimens should be obtained by aggressive approaches if the index of suspicion is high. …”
Get full text

Guar gum-modified magnetic palladium nanocatalyst: C–N coupling reaction and STAT3-Mediated breast cancer therapy by Junrui Luan, Xin Dong, Shuwen Zhou

“…Finally, the Fe3O4@guar gum/Pd NPs showed suppressive properties on colony formation. According to our research, the Fe3O4@guar gum/Pd NPs can decrease STAT3 expression in the treated cells while increasing p53 levels. …”
Get full text