Assessment of the Heterotrophic Bacterial Contamination in Waterlines of Dental Units as a Potential Health Risk Factor in Occupational and Patient’s Exposures by Ali Shahryari, Amineh Zahed, Abdolhalim Rajabi, Maryam Shafipour, Niloufar Emrani, Hadi Rahimzadeh, Hamidreza Heidari

“…Bacterial contamination in the waterlines, dental handpieces, and other water-related components of dental units was investigated and analyzed in terms of Colony-Forming Units per milliliter (CFU/ml). The data were analyzed using the Kruskal-Wallis and Wilcoxon signedrank tests using SPSS version 23.Results: The mean and standard deviation of bacterial contamination across all studied departments were significantly higher than the recommended limit of 500 CFU/ml (ranging from 10,250.00±8,924.28 CFU/ml for the inlet to the water tank in the pediatric department to 275,625.00±41,520.86 CFU/ml for the air-water syringe before flushing in the surgery department). …”
Get full text

Microwave hyperthermia enhances radiosensitization by decreasing DNA repair efficiency and inducing oxidative stress in PC3 prostatic adenocarcinoma cells by Yajun Wu, Pengyuan Liu, Wendy Chen, Shiting Bai, Sisi Chen, Jianglin Chen, Xiaogang Xu, Jindan Xia, Yufei Wu, Jianjun Lai, Chuan Sun, Zhenghong Lao, Xiaoqing Wan, Zhibing Wu

“…We evaluated PC3 cell proliferation using CCK-8, colony experiments, DAPI staining, comet assay and ROS detection method. …”
Get full text

Immortalized Mesenchymal Stem Cells: A Safe Cell Source for Cellular or Cell Membrane-Based Treatment of Glioma by Yuxuan Zhang, Jie Liu, Yunzhao Mo, Zetao Chen, Taoliang Chen, Yan Li, Yaofeng Zheng, Shaokang Deng, Xiangdong Xu, Huajian Chen, Haoqi He, Jiansheng Chen, Tao Jin, Xinlin Sun, Yiquan Ke, Jihui Wang

“…Engineered cells retained the immunophenotype of MSC; im-MSCs maintained the ability to differentiate into mesenchymal lineages in vitro; and im-MSCs showed stronger proliferative capacity than unengineered MSCs but without colony formation in soft agar, no tumorigenicity in the brain, and normal chromosomes. …”
Get full text

Transcription Factor E2F-1 Is Upregulated in Human Gastric Cancer Tissues and Its Overexpression Suppresses Gastric Tumor Cell Proliferation by Qiang Xiao, Lei Li, Yubo Xie, Ning Tan, Changqing Wang, Junyi Xu, Keyi Xia, Kevin Gardner, Qingdi Q. Li

“…Finally, the influence of E2F-1 overexpression on the growth of human gastric carcinoma MKN-45 cells in vitro was assessed by measuring colony formation, cell survival, and cell cycle progression. …”
Get full text

Generation and characterization of two induced pluripotent stem cell lines (ICGi052-A and ICGi052-B) from a patient with frontotemporal dementia with parkinsonism-17 associated wit... by E. V. Grigor’eva, A. A. Malakhova, E. S. Yarkova, J. M. Minina, Y. V. Vyatkin, J. A. Nadtochy, E. A. Khabarova, J. A. Rzaev, S. P. Medvedev, S. M. Zakian

“…The iPSC lines ICGi052-A and ICGi052-B proliferate stably, form colonies with a morphology characteristic of human pluripotent cells, have a normal diploid karyotype (46,XX), express endogenous alkaline phosphatase and pluripotency markers (OCT4, NANOG, SSEA-4 and TRA-1-60) and are able to differentiate into derivatives of three germ layers: ento-, ecto- and mesoderm. …”
Get full text

3-Hydroxyanthranic acid inhibits growth of oral squamous carcinoma cells through growth arrest and DNA damage inducible alpha by Guifang Gan, Xinxia Zhou, Qiaoping Zheng, Xianfu Gao, Xu Chen, Han Zhang, Jinghao Liu, Zhaopeng Shi, Fuxiang Chen

“…Materials and methods: The effects of 3-HAA on OSCC were examined using CCK-8, colony formation, EdU incorporation assays and xenograft mouse model. …”
Get full text

Copper Resistance in <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> Strains from the Prefecture of Pella, Northern Greece, and a Comparison of the Effectiveness of Several Comme... by Thomas Thomidis, Marianthi G. Pagoulatou, Eleftherios Alexandridis, Evaggelia Mpalantinaki, Dimitrios E. Goumas

“…Additionally, resistant bacterial colonies were observed in four strains (18.2%), suggesting the presence of resistant subpopulations within the broader bacterial community. …”
Get full text

Exploring the role of ELOVLs family in lung adenocarcinoma based on bioinformatic analysis and experimental validation by Zihan Wang, Wenjing Cui, Long Liang, Jingge Qu, Yuqiang Pei, Danyang Li, Ying Luo, Yue Zhang, Yifan Qiu, Yongchang Sun

“…Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, cell counting kit-8 (CCK8), colony formation, wound healing, transwell migration assays and fatty acid metabolism detection were employed to confirm the significant role of ELOVL6 in vitro experiment. …”
Get full text

VdPAT1 encoding a pantothenate transporter protein is required for fungal growth, mycelial penetration and pathogenicity of Verticillium dahliae by Stephen Mwangi Kamau, Yongtai Li, Tiange Sun, Feng Liu, Qian-Hao Zhu, Xinyu Zhang, Jie Sun, Yanjun Li

“…In previous study, we identified a pantothenate transporter gene (VdPAT1) in V. dahliae which can be induced by root exudates from a susceptible cotton variety.MethodsIn this study, we generated VdPAT1 deletion mutants and complementary strain via homologous recombination by a PEG-mediated transformation method and used for the gene functional characterization.Results and discussionThe VdPAT1 deletion mutants displayed reduced colony growth, melanin production, spore yield and germination rate, showed abnormal mycelial branching and decreased ability of mycelial penetration and utilization of nutrients (carbon, amino acids and vitamin), leading to a lower pathogenicity. …”
Get full text

p18 encoded by FgGMTV1 is responsible for asymptomatic infection in Fusarium graminearum by Lihang Zhang, Pengfei Li, Yanfei Wang, Shuangchao Wang, Lihua Guo

“…Remarkably, strains with a p18 null mutation show a significant reduction in colony expansion, conidial production, and virulence, leading to a hypovirulent phenotype. …”
Get full text

MicroRNA-21 as a Regulator of Cancer Stem Cell Properties in Oral Cancer by Milica Jaksic Karisik, Milos Lazarevic, Dijana Mitic, Maja Milosevic Markovic, Nicole Riberti, Drago Jelovac, Jelena Milasin

“…CD44+ cells possessed a significantly higher migration and invasion potential compared to CD44− cells, higher levels of miRNA-21 (<i>p</i> = 0.004) and β-catenin (<i>p</i> = 0.005), and lower levels of BAX (<i>p</i> = 0.015). miRNA-21 inhibition in CD44+ cells reduced migration, invasion, and colony formation while increasing apoptosis. Stemness markers were significantly downregulated following miRNA-21 inhibition: <i>OCT4</i> (<i>p</i> = 0.013), <i>SOX2</i> (<i>p</i> = 0.008), and <i>NANOG</i> (<i>p</i> = 0.0001), as well as β-catenin gene (<i>CTNNB1)</i> (<i>p</i> < 0.05), an important member of WNT signaling pathway. …”
Get full text

Preclinical studies of the falnidamol as a highly potent and specific active ABCB1 transporter inhibitor by Baojie Liu, Yongzheng Lu, Ruihui Lin, Junbao Xu, Zilin Shang, Xinyu Hou, Xulong Shao, Zhifang Pan, Tao Yu, Weiguo Feng

“…Methods The reversal effect of falnidamol on MDR was assessed by MTT, colony formation, 3D microsphere, and xenograft model assays. …”
Get full text

RETRACTED ARTICLE: CKS2 induces autophagy-mediated glutathione metabolic reprogramming to facilitate ferroptosis resistance in colon cancer by Leilei Yang, Chengfeng Fang, Jiaju Han, Yufeng Ren, Zaiping Yang, Lingyan Shen, Dinghai Luo, Ruili Zhang, Yan Chen, Shenkang Zhou

“…The in vitro and in vivo effects of CKS2 on CC cells were assessed through the CCK-8 assay, colony formation assay, propidium iodide (PI) staining, BODIPY staining, DCFH-DA staining, and animal experiments. …”
Get full text

Targeted inhibition of the PTEN/PI3K/AKT pathway by YSV induces cell cycle arrest and apoptosis in oral squamous cell carcinoma by Lin Qiu, Qian Gao, Yiheng Liao, Xinxin Li, Cuiying Li

“…Methods A series of in vitro experiments were conducted to assess the impact of YSV on OSCC cell viability, colony formation, cell cycle, and apoptosis. RNA sequencing (RNA-seq), molecular docking, and western blotting were employed to investigate the molecular mechanisms. …”
Get full text

Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker by Siqi Yao, Lingping Tan, Huan Chen, Xiaojun Huang, Wei Zhao, Yan Wang

“…Replicative lifespan determination and colony-forming efficiency assessment were using to assay proliferation capability. …”
Get full text

circSLC6A6 Sponges miR-497-5p to Promote Endometrial Cancer Progression via the PI4KB/Hedgehog Axis by Juan Hu, Xing Peng, Weina Du, Yichuan Huang, Chun Zhang, Xian Zhang

“…The luciferase reporter assay was performed to explore the connection between circSLC6A6 and miR-497-5p as well as the connection between miR-497-5p and PI4KB. The colony formation assay, EdU assay, wound healing assay, and transwell assay were performed to examine the proliferation, migration, and invasion of EC cells. …”
Get full text

Inhibitory effects of limonin on the proliferation of non-small cell lung cancer cells via targeting S-phase kinase-associated protein 2 by Zhu Jiaxian, Zhang Mengting, Li Shengqing, Liu Yongqiang

“…Then, flow cytometry was used to detect the cell cycle and apoptosis, while colony formation assay assessed the low-density proliferation ability of NSCLC cells. …”
Get full text

Endoplasmic reticulum stress-related CLIP4 plays a procarcinogenic role in hepatocellular carcinoma: an integrated analysis by Anqi Wang, Sitong Yan, Weijia Jiang, Xiang Chen, Yuhan Huang, Xiangyu Zu, Xiao Du, Lulu Fan, Jiatao Liu, Guoping Sun

“…Public databases and miRNA-seq data were used to explore the TF-miRNA-CLIP4 regulatory network. CCK-8, colony formation, EdU staining, wound-healing, Transwell, western blotting and RT-qPCR were used to detect the effects of CLIP4 on the proliferation, migration and epithelial-mesenchymal transition (EMT) of HCC cells. …”
Get full text

CircCCT2/miR-146a-5p/IRAK1 axis promotes the development of head and neck squamous cell carcinoma by Long He, Lanruo Li, Liting Zhao, Xiaoya Guan, Yujia Guo, Qi Han, Huina Guo, Hongliang Liu, Chunming Zhang

“…CircCCT2 was characterized by Sanger sequencing, qRT-PCR, RNase R & Actinomycin D treatment, nucleoplasmic separation and FISH experiments. CCK-8 and colony formation assays were performed to determine cell proliferation, and Transwell assays were used to determine migration and invasion. …”
Get full text

Preparation of Sea-buckthorn Oil Microcapsule Composite Film and the Preservation Effect of Hand-grabbed Mutton by Bingbing ZHANG, Guishan LIU, Delang XIE, Haiyang MA, Yang LI, Fang MA, Xiaojun LI, Qingqing CAO, Xiaoxue LI, Qian ZHANG

“…The effects of composite membrane on color, pH, weight loss, TVB-N, total number of bacterial colonies and sensory indexes were analyzed to evaluate the fresh-keeping effect of hand-grabbed mutton. …”
Get full text