RNA tertiary structure and conformational dynamics revealed by BASH MaP by Maxim Oleynikov, Samie R Jaffrey

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The Unpaved Road of Non-Coding RNA Structure–Function Relationships: Current Knowledge, Available Methodologies, and Future Trends by Ana Lúcia Leitão, Francisco J. Enguita

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Intersubunit communication in glycogen phosphorylase influences substrate recognition at the catalytic sites by Nahori Kamada, Ayato Ikeda, Yasushi Makino, Hiroshi Matsubara

Subjects: “…Chemical probing…”
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Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice by Jiho Song, Tianqu Zhai, Janggun Jo, Jae Won Chang

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Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry by Coralie Berthoux, Pablo E. Castillo, Kaoutsar Nasrallah

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Protocol for dual-optical mapping of voltage and calcium sensors in human pluripotent stem cell-derived cardiomyocytes by Faisal J. Alibhai, Arya Masoumi, Ho-June Kim, Nathan Kim, Wesley Kim, Juliana Gomez-Garcia, Peter Lee, Michael A. Laflamme

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Protocol for immunofluorescent staining, high-resolution imaging, and spatial projection of Schlemm’s canal in mouse whole-mount corneal limbus tissue by Weifa Wang, Peiyao Peng, Wenfo Hu, Xuyang Liu, Liming Yang, Rong Ju, Feng Zhang

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Protocol for measuring D-amino acid transport in mammalian cells using a fluorescent biosensor by Danisha Johal, Keeley G. Hewton, Sadie J. Gale, Amritpal S. Johal, Seth J. Parker

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Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting by Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt

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Protocol for high-resolution optical mapping of ex vivo rat heart for electrophysiological studies by Yen Ling Sung, Yuan Si Tsai, Chu Ling Chang, Yu Feng Hu

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Protocols for monitoring condensate formation and dynamics between the phase-separating proteins SET/TAF-Iβ and cytochrome c by Miguel Á. Casado-Combreras, Miguel A. De la Rosa, Irene Díaz-Moreno

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Protocol for synthesizing both racemic and chiral allenylsilanes through copper-catalyzed silylation of propargyl dichlorides by Ying Zhou, Zhengli Liu

Subjects: “…Molecular/Chemical Probes…”
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Protocol to analyze tRNA and rRNA processing using biotin-labeled probes by Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt

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Probing and manipulating the gut microbiome with chemistry and chemical tools by Pavan K. Mantravadi, Basavaraj S. Kovi, Sabbasani Rajasekhara Reddy, Ganesh Pandian Namasivayam, Karunakaran Kalesh, Anutthaman Parthasarathy

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Mollugin reacts with phenol thiol but not produce modification on cysteine discovered by a phenol thiol probe by Tao Liu, Tao Liu, Ruibing Qi, Ruibing Qi, Wanshi Feng, Wanshi Feng, Zhuohao Li, Zhuohao Li, Zerun Zhong, Zerun Zhong, Chuan Bai, Chuan Bai, Chuan Bai

Subjects: “…nucleophilic chemical probe…”
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Protocol for detecting neuronal subnetworks from in vivo calcium imaging data using multiple clustering algorithms in MATLAB by Jui-Yen Huang, Gautam Chauhan, Pei-Ying Chen, Esen Tuna, Hui-Chen Lu

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Split-and-pool synthesis to generate scalable combinatorial oligonucleotide libraries on magnetic nanoparticles by John V.L. Nguyen, Lidija Malic, Christina Nassif, Dillon Da Fonte, Maryam Tabrizian

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Protocol for assembling photostable fluorescence-labeled tiny DNA origami blocks to observe kinesin cooperation by Tomoki Kita, Ryota Sugie, Yuki Suzuki, Shinsuke Niwa

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Enhancing Drug Discovery and Development through the Integration of Medicinal Chemistry, Chemical Biology, and Academia-Industry Partnerships: Insights from Roche’s Endocannabinoid... by Johannes Aebi, Kenneth Atz, Simon M. Ametamey, Jörg Benz, Julie Blaising, Stefania Butini, Giuseppe Campiani, Erick M. Carreira, Ludovic Collin, Eva de Lago, Thais Gazzi, Jürg Gertsch, Luca Gobbi, Wolfgang Guba, Javier Fernández-Ruiz, Jürgen Fingerle, Achi Haider, Yingfang He, Laura H. Heitman, Michael Honer, Daniel Hunziker, Bernd Kuhn, Mauro Maccarrone, Hans Peter Märki, Rainer E. Martin, Peter Mohr, Linjing Mu, Marc Nazaré, David F. Nippa, Sergio Oddi, Fionn O’Hara, Pal Pacher, Julian Romero, Stephan Röver, Arne C. Rufer, Roger Schibli, Gisbert Schneider, Antonia F. Stepan, David A. Sykes, Christoph Ullmer, Mario van der Stelt, Dmitry B. Veprintsev, Matthias B. Wittwer, Uwe Grether

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