Protocol for real-time measurement of mitochondrial bioenergetics in 3D-cultured brain tumor stem cells using the Resipher system by Cloé Tessier, Maxime Toujas, Antonio C. Pagano Zottola, Andreas Bikfalvi, Thomas Mathivet, Thomas Daubon, Lucie Brisson, Audrey Burban, Ahmad Sharanek

Subjects: “…Cell-based Assays…”
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Protocol for genetic dissection of class switch recombination using genome-editing tools by Pengfei Dai, Tingting Liu, Dingpeng Yang, Yifeng Luo, Liu Daisy Liu, Xia Xie, Yafang Shang, Xiaojing Liu, Fei-Long Meng

Subjects: “…cell-based assays…”
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Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion by Wang Xu, Narendra Kumar Gonepudi, Maolin Lu

Subjects: “…Cell-based Assays…”
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Protocol for affinity enrichment of polyclonal autoantibodies from human plasma by Aws Aljnabi, Kelly A. Hagadorn, Louisa E. Neumann, Mary E. Peterson, Bethany Scott, Kazutoyo Miura, Aissata Ongoiba, Safiatou Doumbo, Didier Doumtabe, Shanping Li, Kassoum Kayentao, Boubacar Traore, Silvia Bolland, Peter D. Crompton, Christine S. Hopp

Subjects: “…Cell-based Assays…”
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Protocol for the colocalization of yeast peroxisomal membrane proteins and their binding partners using stimulated emission depletion microscopy by Frank N. Mol, Eline M.F. de Lange, Ida J. van der Klei, Rifka Vlijm

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Protocol for assessing T cell receptor-mediated human T cell cytotoxicity by Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao

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Protocol to rapidly screen CRISPR-Cas9 gene editing outcomes in a cell population by mutating eGFP to a blue or non-fluorescent phenotype by Danny Wilbie, Enrico Mastrobattista, Olivier Gerrit de Jong

Subjects: “…Cell-based Assays…”
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Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures by Stina Oredsson, Atena Malakpour-Permlid, Johan Zhu, Tilo Weber

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Protocol to perform multiplexed assays of variant effect using curated loci prime editing by Carina G. Biar, Nicholas Bodkin, Gemma L. Carvill, Jeffrey D. Calhoun

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Protocol to study internalization and localization dynamics of exogenously added proteins in detergent-permeabilized human cells by Sritama Ray, Kamalika Mukherjee, Suvendra N. Bhattacharyya

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Protocol to identify small-molecule inhibitors against cancer drug resistance by Juan Wei, She-Yu Zhang, Xue-Yuan Zhou, Yong Wei, Hao-Ran Jia, Qin Wu, Weihong Tan

Subjects: “…cell-based assays…”
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Protocol for odorant-binding protein 2A gene knockdown by dual siRNA transfection in a three-dimensional human epidermal equivalent model by Shinobu Nakanishi

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Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique by Tomofumi Yamamoto, Fumihiko Urabe, Yusuke Yoshioka, Yusuke Yamamoto, Takahiro Ochiya

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Protocol to profile tumor and microenvironment from ovarian cancer patient samples and evaluate cell-based therapy using in vitro killing assays by Yan-Ruide Li, Christopher J. Ochoa, Zibai Lyu, Yichen Zhu, Yuning Chen, Gabriella DiBernardo, Lauryn Ruegg, Sanaz Memarzadeh, Lili Yang

Subjects: “…Cell-based Assays…”
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Protocol for characterizing cell motility and extracellular signal-regulated kinase dynamics in epithelial monolayers using FRET imaging data by Julio Cesar Sanchez-Rendon, Lara Hundsdorfer, Effie E. Bastounis

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Protocol for measuring mitochondrial respiratory capacity from buccal cell swabs by Tina R. Ram, Chunlong Mu, Jaclyn C. Campbell, Sarah J. MacEachern, Jane Shearer

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Protocol for in-plate staining of clinical peripheral blood mononuclear cell samples for high-throughput analysis via spectral flow cytometry by Isabella Marciello, Sonya Feng, Ilana Gomez Diaz, Julia Huezo, F. Stephen Hodi, Maria Cimpean, Joanna Baginska

Subjects: “…Cell-based Assays…”
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Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system by Daniel Ivanusic, Norbert Bannert

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Protocol for analysis of miRNAs in human melanoma cells by Evelyn Lattmann, Tessa Guggiari, Patrick Turko, Mitchell P. Levesque

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Protocol for isolating and purifying murine liver sinusoidal endothelial cells for in vitro culture and functional assays by Sophia Papaioannou, Jia-Xiang See, Tinja Baljkas, Philipp Reiners-Koch, Manuel Winkler, Adelheid Cerwenka, Ana Stojanovic

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