Halothermophilic Cas9 and dCas9 Interaction with Variety sgRNA In-silico by Kenny Lischer, Kholisoh Hayati, Muhammad Haykal Fabian, Fina Amreta Laksmi, Yudhi Nugraha, Hamdan Dwi Rizqi, Riri Fauziyya, Sarmoko, Mohd Shukuri Mohamad Ali

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Engineering of SauriCas9 with enhanced specificity by Xiaoqi Zhang, Chen Tao, Miaomiao Li, Sufang Zhang, Puping Liang, Yan Huang, Huihui Liu, Yongming Wang

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CRISPR/Cas9-editing of PRNP in Alpine goats by Aurélie Allais-Bonnet, Christophe Richard, Marjolaine André, Valérie Gelin, Marie-Christine Deloche, Aurore Lamadon, Gwendoline Morin, Béatrice Mandon-Pépin, Eugénie Canon, Dominique Thépot, Johann Laubier, Katayoun Moazami-Goudarzi, Ludivine Laffont, Olivier Dubois, Thierry Fassier, Patrice Congar, Olivier Lasserre, Tiphaine Aguirre-Lavin, Jean-Luc Vilotte, Eric Pailhoux

“…The aim of this work was to engineer Alpine goats carrying knockout (KO) alleles of PRNP, the PrPc-encoding gene, using CRISPR/Cas9-ribonucleoproteins and single-stranded donor oligonucleotides. …”
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Structural insights into how Cas9 targets nucleosomes by Reina Nagamura, Tomoya Kujirai, Junko Kato, Yutaro Shuto, Tsukasa Kusakizako, Hisato Hirano, Masaki Endo, Seiichi Toki, Hiroaki Saika, Hitoshi Kurumizaka, Osamu Nureki

“…Despite previous structural analyses focusing on Cas9 cleaving free DNA, structural insights into Cas9 targeting of DNA within nucleosomes are limited, leading to uncertainties in understanding how Cas9 operates in the eukaryotic genome. …”
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Enhanced genome editing with a Streptococcus equinus Cas9 by Jingtong Liu, Yao Wang, Jingjing Wei, Shengzhou Wang, Miaomiao Li, Zheyong Huang, Sufang Zhang, Huihui Liu, Jinhai Huang, Yongming Wang

“…Abstract A large number of SpCas9 orthologs has been computationally identified, but their genome editing potential remains largely unknown. …”
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CRISPR-Cas9 in basic and translational aspects of cancer therapy by Maryam Samareh Salavatipour, Zahra Poursalehi, Negin Hosseini Rouzbahani, Sohaib Mohammadyar, Mohammad Vasei

Subjects: “…crispr-cas9…”
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CRISPR/Cas9, a universal tool for genomic engineering by A. V. Smirnov, A. M. Yunusova, V. A. Lukyanchikova, N. R. Battulin

Subjects: “…crispr/cas9…”
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CRISPR/Cas9: Role of genome editing in cancer immunotherapy by S. Vimal, Inamul Hasan Madar, Logalakshmi Thirumani, Lakshmi Thangavelu, Azhagu Madhavan Sivalingam

Subjects: “…CRISPR/Cas9…”
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Obtaining a pancreatic cancer cell line stably expressing doxycycline-dependent endonuclease Cas9 by A.K. Nurgalieva, V.S. Skripova, L.F. Minigulova, R.G. Kiyamova

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Flexibility in PAM recognition expands DNA targeting in xCas9 by Kazi A Hossain, Lukasz Nierzwicki, Modesto Orozco, Jacek Czub, Giulia Palermo

Subjects: “…CRISPR-Cas9…”
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Intein-mediated split SaCas9 for genome editing in plants by Danling Hu, Lizhe Hu, Yaqiang Lu, Xiao Dong, Xingyu Cao, Shasha Bai, Lingang Zhang, Dongming Li, Yongwei Sun

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CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding by Qihong Yang, Liangyu Cai, Mila Wang, Guiyun Gan, Weiliu Li, Wenjia Li, Yaqin Jiang, Qi Yuan, Chunchun Qin, Chuying Yu, Yikui Wang

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DNA targeting by compact Cas9d and its resurrected ancestor by Rodrigo Fregoso Ocampo, Jack P. K. Bravo, Tyler L. Dangerfield, Isabel Nocedal, Samatar A. Jirde, Lisa M. Alexander, Nicole C. Thomas, Anjali Das, Sarah Nielson, Kenneth A. Johnson, Christopher T. Brown, Cristina N. Butterfield, Daniela S. A. Goltsman, David W. Taylor

“…Recently, CRISPR-Cas systems with highly compact nucleases have been discovered, including Cas9d (a type II-D nuclease). Here, we report the cryo-EM structures of a Cas9d nuclease (747 amino acids in length) in multiple functional states, revealing a stepwise process of DNA targeting involving a conformational switch in a REC2 domain insertion. …”
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CRISPR-Cas9 in Cardiovascular Medicine: Unlocking New Potential for Treatment by Klaudia Bonowicz, Dominika Jerka, Klaudia Piekarska, Janet Olagbaju, Laura Stapleton, Munirat Shobowale, Andrzej Bartosiński, Magdalena Łapot, Yidong Bai, Maciej Gagat

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Genome editing using CRISPR/ Cas9 system: a practical guide by A. G. Menzorov, V. A. Lukyanchikova, A. N. Korablev, I. A. Serova, V. S. Fishman

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Genetic Improvement of rice Grain size Using the CRISPR/Cas9 System by Tao Zhang, Zhengwei Wang, Qiaoquan Liu, Dongsheng Zhao

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基于episomal CRISPR/Cas9 载体的基因敲入方法 by 邹征伟, 赖兴强, 李伟强

Subjects: “…CRISPR/Cas9…”
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Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells by Zixi Yin, Lingyi Chen

“…The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. …”
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Molecular Mechanisms of Breast Cancer Drug Resistance and CRISPR/Cas9 Strategies to Overcome by Bashdar Mahmud Hussen, Bnar Saleh Ismael, Saman S. Abdulla, Noor Haval Jamal, Suhad Asad Mustafa, Zana Baqi Najmalddin, Mohammed Fatih Rasul

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A Review: CRISPR Cas System and the Mechanism With an Inhibition of Binding of CRISPR Cas‐9 by Akram Hosen, Md Nakibul Hasan Nishat, MD. Mohaimin Hossain Soaib, Omar Sharif Sharkar, Md. Sahabuddin, Imdadul Haque Sharif, Sujay Kumar Bhajan

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