JK-1, a useful erythroleukemic cell line model to study a controlled erythroid differentiation from progenitors to terminal erythropoiesis by Sylvain Metral, Sandrine Genetet, Benoît Gamain, Isabelle Mouro-Chanteloup

“…The possibility of obtaining large quantities of JK-1 cells at each stage of differentiation, as shown in this study, as well as the potential to genetically modify these cells, via CrisprCas9, makes their use of considerable interest for studying pathologies occurring during erythropoiesis.…”
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The BnaBPs gene regulates flowering time and leaf angle in Brassica napus by Jiang Yu, Yi‐Xuan Xue, Rehman Sarwar, Shi‐Hao Wei, Rui Geng, Yan‐Feng Zhang, Jian‐Xin Mu, Xiao‐Li Tan

“…The protein sequence analysis showed two proteins containing conserved domains KNOX I, KNOX II, ELK, and HOX of the KONX protein family. The CRISPR/Cas9 knockout lines exhibited early budding and flowering time, coupled with floral organ abscission earlier and a larger leaf angle. …”
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Live Visualization of Calcified Bones in Zebrafish and Medaka Larvae and Juveniles Using Calcein and Alizarin Red S by Rina Koita, Sae Oikawa, Taisei Tani, Masaru Matsuda, Akinori Kawamura

“…Zebrafish and medaka are valuable model vertebrates for genetic studies. The advent of CRISPR-Cas9 technology has greatly enhanced our capability to produce specific gene mutants in zebrafish and medaka. …”
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Casein Kinases 2-dependent phosphorylation of the placental ligand VAR2CSA regulates Plasmodium falciparum-infected erythrocytes cytoadhesion. by Dominique Dorin-Semblat, Jean-Philippe Semblat, Romain Hamelin, Anand Srivastava, Marilou Tetard, Graziella Matesic, Christian Doerig, Benoit Gamain

“…Furthermore, using CRISPR/Cas9 we generated a parasite line in which phosphoresidue S1068 was changed to alanine. …”
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A strategy for controlling Hypervirulent Klebsiella pneumoniae: inhibition of ClpV expression by Wenke Liu, Dan Wang, Qiangxing He, Shiwen Cao, Jiaxin Cao, Huajie Zhao, Junwei Cui, Fan Yang

“…In the present study, the clpV gene deletion strain of hvKP (ΔclpV-hvKP) was constructed using CRISPR-Cas9 technology, and the biological characteristics of ΔclpV-hvKP were investigated to explore the new targets for controlling this pathogen. …”
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Knockout or inhibition of DHPS suppresses ovarian tumor growth and metastasis by attenuating the TGFβ pathway by Guannan Zhao, Xinxin Zhao, Ziping Liu, Baojin Wang, Peixin Dong, Hidemichi Watari, Lawrence M. Pfeffer, Gabor Tigyi, Wenjing Zhang, Junming Yue

“…Using lentiviral CRISPR/Cas9 vectors for DHPS knockout, we observed EMT inhibition in SKOV3 and OVCAR8 cells through suppressed hypusination and reduced EIF5A2 expression. …”
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REZILIENȚA CULTURII DE FLOAREA-SOARELUI ÎN CONDIȚII DE STRES BIOTIC by USM ADMIN

“…Tehnologiile emergente, cum ar fi CRISPR-Cas9, oferă oportunități promițătoare pentru crearea unor genotipuri de floarea-soarelui mai rezistente. …”
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Subtilases: a major prospect to the genome editing in horticultural crops by Umashankar Chandrasekaran, Umashankar Chandrasekaran, Woo Jong Hong, Hyeran Kim, Hyeran Kim

“…This review summarizes recent findings on SBT enzymes, which act as ‘protein scissors’ in activating peptide hormones, and discusses the potential for using selected SBTs in CRISPR-Cas9 gene editing to enhance the growth and resilience of economically important Solanaceae crops, with a focus on pepper.…”
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Biotechnology breakthroughs: Revolutionizing oral cancer treatment by Safin Hussein, Karzan Qurbani, Haider Hamzah, Seenaa Ali, Sirwan Khalid Ahmed

“…Oncolytic viruses and CRISPR-Cas9 are among the tools leading the way in personalized interventions. …”
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The role of microRNAs in learning and long-term memory by L. N. Grinkevich

“…In addition, analysis is provided of the current literature related to the prospects of improving cognitive processes by influencing microRNA biogenesis via the use of CRISPR/Cas9 technologies and active mental and physical exercises.…”
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Light quality‐dependent roles of REVEILLE proteins in the circadian system by Cassandra L. Hughes, Yuyan An, Julin N. Maloof, Stacey L. Harmer

“…To clarify the function of the REVEILLE 4, REVEILLE 6, and REVEILLE 8 transcriptional activators, we characterized the growth and clock phenotypes of CRISPR‐Cas9‐generated single, double, and triple rve mutants. …”
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DNA Ligase IV Prevents Replication Fork Stalling and Promotes Cellular Proliferation in Triple Negative Breast Cancer by Rashmi R. Joshi, Sk Imran Ali, Amanda K. Ashley

“…In addition, Lig4 is an attractive clinical target for directing CRISPR/Cas9-mediated repair towards homology-directed repair and away from NHEJ, thus understanding of how diminishing Lig4 impacts cell biology is critical.…”
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Heterozygous mutation in BRCA2 induces accelerated age-dependent decline in sperm quality with male subfertility in rats by Yashiro Motooka, Hideaki Tanaka, Yuki Maeda, Misako Katabuchi, Tomoji Mashimo, Shinya Toyokuni

“…Here, we established a rat model with a frameshift on the seventh BRC repeat in Brca2 exon 11 (Brca2 +/p.T1942fs), which is homologous to human BRCA2 +/p.T1974fs, using CRISPR/Cas9 system. Our analyses revealed that the heterozygous rats with the PV in the BRCA2 exon 11 showed increased DNA double-strand breaks and apoptosis in spermatogonia and spermatocytes, accelerated testicular germ cell loss, and deterioration in sperm quality according with aging, ultimately resulting in early male reproductive dysfunction. …”
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应用双靶标CRISPR慢病毒载体在间充质干细胞中稳定敲除lncRNA DANCR基因 by 彭丹, 钟小敏

“…【目的】应用CRISPR/Cas9方法，构建特异性靶向长链非编码RNADANCR基因两端的双靶标慢病毒载体，稳定敲除间充质干细胞（MSC）中的DANCR基因，为研究DANCR的生物学功能奠定基础。…”
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A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans. by Haania Khan, Xinyu Huang, Vishnu Raj, Han Wang

“…We designed a cGAL gene trap cassette and used CRISPR/Cas9 to insert it into the target gene, creating a bicistronic operon that simultaneously expresses a truncated endogenous protein and the cGAL driver in the cells expressing the target gene. …”
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Knockout of the ING5 epigenetic regulator confirms roles in stem cell maintenance and tumor suppression in vivo. by Buthaina Al Shueili, Arthur Dantas, Etienne Mahe, Tak Ho Chu, Yang Yang, Elodie Labit, Eren Kutluberk, Nicolas Lasaleta, Anand Masson, Hiba Omairi, Kenichi Ito, Roman J Krawetz, Rajiv Midha, Gregory Cairncross, Karl Riabowol

“…Here we find that CRISPR/Cas9 ING5 knockout (KO) mice are sub-fertile but show no decrease in lifespan or ability to heal wounds despite indications of depleted stem cell pools in several tissues. …”
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Increased distribution of carbon metabolic flux during de novo cytidine biosynthesis via attenuation of the acetic acid metabolism pathway in Escherichia coli by Tong Ye, Wei Ding, Zhengxu An, Haojie Zhang, Xiaobo Wei, Junnan Xu, Huiyan Liu, Haitian Fang

“…To mitigate carbon source loss, the CRISPR-Cas9 gene-editing technique was employed to knock out the poxB and pta genes in E. coli, generating the engineered strains K12ΔpoxB and K12ΔpoxBΔpta. …”
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Glycosylation of ceramide synthase 6 is required for its activity by Alexandra J. Straus, Grace Mavodza, Can E. Senkal

“…We used a robust HCT116 CRISPR-Cas9 CerS6 KO with reintroduction of either WT CerS6 or a mutant CerS6 with a point mutation at asparagine-18 to an alanine (N18A) which abrogated glycosylation at that residue. …”
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<i>Macrostomum lignano</i> as a model to study the genetics and genomics of parasitic flatworms by K. V. Ustyantsev, V. Yu. Vavilova, A. G. Blinov, E. V. Berezikov

“…All this makes M. lignano amenable to the most modern approaches of forward and reverse genetics, such as transposon insertional mutagenesis and methods of targeted genome editing by the CRISPR/Cas9 system. Due to the availability of an increasing number of genome and transcriptome assemblies of different parasitic flatworm species, new knowledge generated by studying M. lignano can be easily translated to parasitic f latworms with the help of modern bioinformatic methods of comparative genomics and transcriptomics. …”
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Lnc-DC promotes estrogen independent growth and tamoxifen resistance in breast cancer by Wan-Xin Peng, Pratirodh Koirala, Huaixiang Zhou, Jiahong Jiang, Ziqiang Zhang, Liu Yang, Yin-Yuan Mo

“…The purpose of this study was to determine whether long non-coding RNAs (lncRNAs) are involved in regulation of estrogen independent growth and tamoxifen resistance in ER positive breast cancer. Using a CRISPR/Cas9-based SAM (synergistic activation mediator) library against a focus group of lncRNAs, we identify Lnc-DC as a candidate lncRNA. …”
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