A conserved element in the first intron of Cd4 has a lineage specific, TCR signal-responsive, canonical enhancer function that matches the timing of cell surface CD4 upregulation r... by Gregory A. Swan, Gregory A. Swan, Chika Fujii, Mia E. Guzynski, Sheridan M. Page, Isabelle V. Meyers, Yordan P. Penev, Sejiro Littleton, Sejiro Littleton, Adinda Azzahra, Christine Richardson, Sophia D. Sarafova, Sophia D. Sarafova

“…EGFP mRNA levels were measured via RT-qPCR, and EGFP fluorescence was analyzed in post-selection thymocytes.ResultsOur in vitro experiments demonstrate that NCE by itself can function as an enhancer at the INT, but not the DP stage of development. Furthermore, CRISPR/Cas9-mediated deletion of coreNCE/E4m resulted in reduced CD4 surface levels, slower re-expression rates, and reduced TCR signaling responsiveness in INT cells, but not in DP cells. …”
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Nanomédicaments pour le traitement de maladies graves et recherches duales by Couvreur, Patrick

“…This review aims to describe and discuss the characteristics and functionalities of the different generations of nanocarriers, from their inception to the present day, discussing the prospects they offer for the production of therapeutic proteins, for facilitating gene editing (CRISPR/Cas9), and for enabling immune checkpoint blockade in oncology. …”
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NF2 can mediate the expression of CAMK2A in a tissue specific manner by Zhaoying Lei, Huajian Cai, Qingfeng Yan

“…Therefore, meningiomas and several non-nerve system tumor types were analyzed using KEGG analysis and CRISPR/Cas 9 technology to determine the role of NF2 in regulating tissue specificity. …”
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Stage-specific expression and divergent functions of two insulinase-like proteases associated with host infectivity in Cryptosporidium. by Yue Huang, Shifeng Pei, Xin Lv, Fuxian Yang, Xiaoqing Gong, Na Li, Yaqiong Guo, Yaoyu Feng, Lihua Xiao

“…<h4>Methodology/principal findings</h4>In this study, we investigated the expression and biological function of the INS-19 and INS-20 of C. parvum. CRISPR/Cas9 was used to endogenously tag both genes with the hemagglutinin epitope. …”
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miRNA changes associated with differentiation of human embryonic stem cells into human retinal ganglion cells by Maryam Esmaeili, Daniel A. Smith, Ben Mead

“…Retinal ganglion cells (RGC), whose axons connect the eye to the brain, are central nervous system cells of great interest, yet their study is largely restricted to animals due to the difficulty in obtaining healthy human RGC. Using a CRISPR/Cas9-based reporter embryonic stem cell line, human RGC were generated and their miRNA profile characterized using NanoString miRNA assays. …”
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A chromosome-level genome assembly of wild silkmoth, Bombyx mandarina by Jung Lee, Takashi Kiuchi, Katsushi Yamaguchi, Shuji Shigenobu, Atsushi Toyoda, Toru Shimada

“…ATAC-seq data comprehensively detected open chromosome regions, which will benefit when CRISPR/Cas9-mediated genome editing is conducted.…”
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The Proviral Reservoirs of Human Immunodeficiency Virus (HIV) Infection by Andrey I. Murzin, Kirill A. Elfimov, Natalia M. Gashnikova

“…Uncovering the causes of this phenomenon will bring humanity closer to curing HIV infection, potential approaches to which include stem cell transplantation, clustered regularly interspaced short palindromic repeats (CRISPR)/cas9, “Shock and kill”, “Block and lock”, and the application of broad-spectrum neutralizing antibodies (bNAbs).…”
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Exploring Novel Non-Operative Treatments for Osteoarthritis: An Examination of Innovative Therapies by Piotr Robert Bakuła, Kamila Jałocha, Joanna Zygadło

“…Cutting-edge technologies such as RNA interference, CRISPR/Cas9, and advanced regenerative medicine are also explored for their potential to revolutionize OA management. …”
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Single-cell RNA sequencing and AlphaFold 3 insights into cytokine signaling and its role in uveal melanoma by Hongyan Sun, Cunzi Li, Zuhui Pu, Zuhui Pu, Ying Lu, Ying Lu, Zijing Wu, Zijing Wu, Lan Zhou, Lan Zhou, Hongzhan Lin, Yumo Wang, Tao Zi, Lisha Mou, Lisha Mou, Ming-ming Yang

“…T-cell populations were analyzed using pseudotime trajectory mapping and interaction network visualization. CRISPR-Cas9 screening analysis was conducted to identify hub genes and cytokine pathways that may serve as therapeutic targets. …”
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Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors by Xiujuan Zhang, Siyuan Hao, Zehua Feng, Kang Ning, Cagla Aksu Kuz, Shane McFarlin, Donovan Richart, Fang Cheng, Ander Zhang-Chen, Richenda McFarlane, Ziying Yan, Jianming Qiu

“…ABSTRACT We conducted a genome-wide CRISPR/Cas9 screen in suspension 293 F cells transduced with rAAV5. …”
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Loss of MEF2C function by enhancer mutation leads to neuronal mitochondria dysfunction and motor deficits in mice by Ali Yousefian-Jazi, Suhyun Kim, Jiyeon Chu, Seung-Hye Choi, Phuong Thi Thanh Nguyen, Uiyeol Park, Min-gyeong Kim, Hongik Hwang, Kyungeun Lee, Yeyun Kim, Seung Jae Hyeon, Hyewhon Rhim, Hannah L. Ryu, Grewo Lim, Thor D. Stein, Kayeong Lim, Hoon Ryu, Junghee Lee

“…MEF2C localizes and binds to the mitochondria DNA, and directly modulates mitochondria-encoded gene expression. CRISPR/Cas-9-induced mutation of the MEF2C enhancer decreases expression of mitochondria-encoded genes. …”
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Knock-In Mice with Myo3a Y137C Mutation Displayed Progressive Hearing Loss and Hair Cell Degeneration in the Inner Ear by Peipei Li, Zongzhuang Wen, Guangkai Zhang, Aizhen Zhang, Xiaolong Fu, Jiangang Gao

“…To investigate the mechanism of DFNB30-type deafness, we established a mouse model of Myo3a kinase domain Y137C mutation by using CRISPR/Cas9 system. No difference in hearing between 2-month-old Myo3a mutant mice and wild-type mice was observed. …”
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Cell-marking techniques for cell lineage tracing by A. M. Yunusova, N. R. Battulin

“…One chapter of the review describes novel advancements in the field of CRISPR/Cas9-based cellular barcoding.…”
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Generation and heterozygous repair of human iPSC lines from two individuals with the neurodevelopmental disorder, TRAPPC4 deficiency by Riley Hall, Tim Sikora, Annabelle Suter, Jia Yi Kuah, John Christodoulou, Nicole J Van Bergen

“…We have generated stem cells from fibroblasts of two individuals with the same homozygous TRAPPC4 c.454 + 3A > G pathogenic variant and used CRISPR/Cas9 editing to generate heterozygous gene-corrected isogenic controls. …”
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Nucleic acid therapeutics: Past, present, and future by Sajid Naeem, Ju Zhang, Yang Zhang, Yu Wang

“…The field of nucleic acid therapeutics has been brought to the forefront by the rapid development of vaccines against COVID-19, followed by a number of approvals for clinical use including much anticipated CRISPR-Cas9. However, obstacles such as the difficulty of achieving efficient and targeted delivery to diseased sites remain. …”
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Rapid and quantitative functional interrogation of human enhancer variant activity in live mice by Ethan W. Hollingsworth, Taryn A. Liu, Joshua A. Alcantara, Cindy X. Chen, Sandra H. Jacinto, Evgeny Z. Kvon

“…Here we introduce dual-enSERT, a robust Cas9-based two-color fluorescent reporter system which enables rapid, quantitative comparison of enhancer allele activities in live mice in less than two weeks. …”
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A Y chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae by Ignacio Tolosana, Katie Willis, Matthew Gribble, Lee Phillimore, Austin Burt, Tony Nolan, Andrea Crisanti, Federica Bernardini

“…The YLE comprises a CRISPR-Cas9 construct that is always inherited by males yet generates an autosomal dominant mutation that is transmitted to over 90% of the offspring and results in female-specific sterility. …”
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Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment by Katherine M. Ranard, Bruce Appel

“…Here, we create a novel DHA-deficient zebrafish model by 1) disrupting elovl2, a key gene in the DHA biosynthesis pathway, via CRISPR/Cas9 genome editing, and 2) feeding mothers a DHA-deficient diet. …”
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Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana. by Anthony Ricou, Matthieu Simon, Rémi Duflos, Marianne Azzopardi, Fabrice Roux, Françoise Budar, Christine Camilleri

“…In this study, we identified the two other genes of the pollen killer by using CRISPR-Cas9 induced mutants. These two genes are necessary for the killer activity that we demonstrated to be specific to pollen. …”
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ParSite is a multicolor DNA labeling system that allows for simultaneous imaging of triple genomic loci in living cells. by Xiaohui He, Yadong Sun, Hanhui Ma

“…A pair of genomic loci labeled by ParSite could be colocalized with p53-binding protein 1 (53BP1) in response to CRISPR/Cas9-mediated double-strand breaks (DSBs). The ParSite permits tracking promoter and terminator dynamics of the APP gene, which spans 290 kilobases in length. …”
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