Huntington’s disease modeling on HEK293 cell line by D. V. Sharipova, T. B. Malankhanova, A. A. Malakhova

“…The new genome editing approaches, such as CRISPR/Cas9, allow us to generate isogenic cell lines that can be useful for drug screening and studying mechanisms of molecular and cellular events triggered by certain mutation on an equal genetic background. …”
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Gene drive-based population suppression in the malaria vector Anopheles stephensi by Xuejiao Xu, Jingheng Chen, You Wang, Yiran Liu, Yongjie Zhang, Jie Yang, Xiaozhen Yang, Bin Chen, Zhengbo He, Jackson Champer

“…Here, we construct a homing suppression drive in the major urban malaria vector Anopheles stephensi targeting the female-specific exon of doublesex, incorporating two gRNAs and a nanos-Cas9 to reduce functional resistance and improve female heterozygote fitness. …”
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Colon Cancer Genetic and Epigenetic Changes, Etiopathogenesis, Risk Factors, Treatment: Literature Review by Gabrielė Ūbaitė, Aurelija Remeikaitė, Justas Žilinskas

“…It highlights the potential of CRISPR/Cas9, immunotherapies, and liquid biopsies in pioneering personalized CRC treatments and early detection. …”
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Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 by Soo-Ji Park, Sungjin Ju, Won Jun Jung, Tae Yeong Jeong, Da Eun Yoon, Jang Hyeon Lee, Jiyun Yang, Hojin Lee, Jungmin Choi, Hyeon Soo Kim, Kyoungmi Kim

“…Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among which Un1Cas12f1 is 2.6 times smaller than SpCas9, providing advantages for gene therapy research. …”
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Hyperthermia and cisplatin combination therapy promotes caspase-8 accumulation and activation to enhance apoptosis and pyroptosis in cancer cells by Guanghui Zi, Jin Chen, Yatu Peng, Yue Wang, Baowei Peng

“…In addition, caspase-8 activation was modulated by CRISPR-Cas9 gene-editing or pharmacological inhibition.Results Combination therapy promoted K63-linked polyubiquitination of caspase-8 and cellular accumulation of caspase-8. …”
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Cysteine Rich Intestinal Protein 2 is a copper-responsive regulator of skeletal muscle differentiation and metal homeostasis. by Odette Verdejo-Torres, David C Klein, Lorena Novoa-Aponte, Jaime Carrazco-Carrillo, Denzel Bonilla-Pinto, Antonio Rivera, Arpie Bakhshian, Fa'alataitaua M Fitisemanu, Martha L Jiménez-González, Lyra Flinn, Aidan T Pezacki, Antonio Lanzirotti, Luis Antonio Ortiz Frade, Christopher J Chang, Juan G Navea, Crysten E Blaby-Haas, Sarah J Hainer, Teresita Padilla-Benavides

“…Deleting mCrip2 using CRISPR/Cas9 disrupted muscle formation due to Cu accumulation. …”
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Targeted Inhibition of the miR-199a/214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Con... by Yumei Luo, Xuehu Xu, Xiuli An, Xiaofang Sun, Shu Wang, Detu Zhu

“…We first developed a baculovirus-delivered CRISPR interference (CRISPRi) system that sterically blocked the E-box element in the promoter of the miR-199a/214 cluster with an RNA-guided catalytically dead Cas9 (dCas9). We then applied this CRISPRi system to hiPS-NSCs and successfully suppressed the expression of miR-199a-5p, miR-199a-3p, and miR-214 in the microRNA gene cluster. …”
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Application and development of CRISPR technology in the secondary metabolic pathway of the active ingredients of phytopharmaceuticals by Haixin Gao, Xinyi Pei, Xianshui Song, Shiying Wang, Zisong Yang, Jianjun Zhu, Qiupeng Lin, Qinlong Zhu, Xiangna Yang

“…As an efficient gene editing tool, the CRISPR/Cas9 system has been widely employed to investigate and regulate the biosynthetic pathways of active ingredients in medicinal plants. …”
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Loss of phosphatase and tensin homolog (PTEN) increases Lysyl oxidase-like 2 (LOXL2) expression enhancing the growth of fallopian tube epithelial cells as three-dimensional spheroi... by Angela Russo, Junlone Moy, Manead Khin, Timothy R. Dorsey, Alfredo Lopez Carrero, Joanna E. Burdette

“…In addition, CRISPR-CAS9 was used to delete LOXL2 in OVCAR8 ovarian cancer cells. …”
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SiCLAT: simultaneous imaging of chromatin loops and active transcription in living cells by Xin Wan, Jie Kong, Xiaodi Hu, Lulu Liu, Yuanping Yang, Hu Li, Gaoao Liu, Xingchen Niu, Fengling Chen, Dan Zhang, Dahai Zhu, Yong Zhang

“…Abstract We present SiCLAT, which introduces a dCas9-dCas13d cassette into the mouse genome. This model enables the stable expression of both dCas9 and dCas13 proteins in diverse cell populations, facilitating concurrent labeling of DNA and RNA across various cell types. …”
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THERAPEUTIC GENE EDITING FOR HEMOGLOBINOPATHIES by Ugo Testa, Giuseppe Leone, Maria Domenica Cappellini

“…Initial studies of gene therapy for hemoglobinopathies involved the use of lentiviral vectors to add functional copies of the gene encoding b-globin in defective CD34 cells; more recently, gene editing techniques have been used involving either CRISPR-Cas9, transcription activation-like effector protein nuclease, zinc finger nuclease, and base editing to either induce fetal hemoglobin production at therapeutic levels or to genetically repair the underlying molecular defect causing the disease. …”
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The Rise of CRISPR/Cas for Genome Editing in Stem Cells by Bing Shui, Liz Hernandez Matias, Yi Guo, Ying Peng

“…Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. …”
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Genomic Analysis Methods of Microorganisms by Canan Kebabçıoğlu, Osman Erganiş

“…CRISPR modifies the genetic code and can correct mutations or control genes using Cas9. These methods are important for identifying bacterial species and annotating genomic information. …”
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Utilisation des Anti-Inflammatoires Non Stéroïdiens chez les personnes âgées en rhumatologie au CHR-Tsévié (Togo) by VES Koffi-Tessio, E Fianyo, ML Diallo, S Oniankitan, A Adjolou, KC Tagbor, KA Nouvedji, M Ngakui, A Tsowou, C Deghela, K Kakpovi, P Houzou, O Oniankitan, M Mijiyawa

“…La pathologie dégénérative du rachis (137 cas, 5­6%), la gonarthrose (43 cas, 18%), les métastases osseuses (22 cas, 9%), les pathologies périarticulaires (23 cas, 9%), les pathologies microcristallines (4 cas, 1,6%), les rhumatismes inflammatoires (5 cas, 2%), l’algodystrophie (2 cas, 0,8%) et les infections (7 cas, 2,9%) ont été les affections ayant motivé l’usage d’AINS. …”
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Fission yeast essential nuclear pore protein Nup211 regulates the expression of genes involved in cytokinesis. by Domenick Kamel, Ayisha Sookdeo, Ayana Ikenouchi, Hualin Zhong

“…Using domain analysis and CRISPR/cas9 technology, we determined that the first 655 residues of Nup211 are sufficient for viability. …”
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A REVIEW ON RECENT ADVANCES IN ANIMAL BIOTECHNOLOGY by Sarada Bhandari, Lokendra Nath Yogi

“…Recent advancements encompass various domains, including genetic editing techniques like CRISPR-Cas9, which allow precise genetic modifications for improved animal health and product quality. …”
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Engineering CRISPR for emerging therapeutic applications by Xiaoqi Sun, Zhuang Liu

“…The discovery of the ability of CRISPR–Cas9 to cut targeted DNA sequences at predetermined sites has introduced a new era of precision and flexibility in genome editing. …”
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Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells by Dingbo Zhang, Fiona Parth, Laura Matos da Silva, Teng-Cheong Ha, Axel Schambach, Jens Boch

“…We report a novel CRISPR/Cas9-cytosine base editor using SsdA, a DYW-like deaminase and bacterial toxin. …”
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mGBP2 engages Galectin-9 for immunity against Toxoplasma gondii. by Elisabeth Kravets, Gereon Poschmann, Sebastian Hänsch, Veronica Raba, Stefanie Weidtkamp-Peters, Daniel Degrandi, Kai Stühler, Klaus Pfeffer

“…Furthermore, we could prove the requirement of Gal9 for growth control of T. gondii by CRISPR/Cas9 mediated gene editing. These discoveries clearly indicate that Gal9 is a crucial factor for the mGBP2-coordinated cell-autonomous host defense mechanism against T. gondii.…”
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Advances in retina genetics: Progress, potential, and challenges by Mayank Bansal

“…Stem cells, gene augmentation, and gene-editing techniques such as CRISPR/Cas9 are discussed, in which we highlight ongoing research as well as their potential in the targeted treatment of retinal diseases. …”
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