Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation by L. G. V. Fernandes, A. L. T. O. Nascimento, J. E. Nally

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Different Approach on our Cells and the Use of CRISPR/Cas 9 Technology: Super Gene'ethic by Ayşenur Taşkaya, Ayşe Nur Akmeşe, Himmet Akkaya, Ece Demirel, Sümeyra Demir, Emine Kav, Şengül Koçer, Elif Senanur Yüksel, Pelin Bacak, Serap Karakaya, Mohammed Saleh Ameen Al Qadri, Hale Köksoy

Subjects: “…crispr/cas9…”
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Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production by Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh

“…The Cas9-BD, a modified Cas9 with the addition of polyaspartate to its N- and C-termini, is developed with decreased off-target binding and cytotoxicity compared with wild-type Cas9. …”
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Generation and propagation of high fecundity gene edited fine wool sheep by CRISPR/Cas9 by Xuemei Zhang, Meiyu Qiu, Bing Han, Li Liao, Xinrong Peng, Jiapeng Lin, Ning Zhang, Lati Hai, Long Liang, Yila Ma, Wenrong Li, Mingjun Liu

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CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1 by Kamilla Wiull, Lisa K. Haugen, Vincent G. H. Eijsink, Geir Mathiesen

“…To deal with this obstacle, we have constructed a finetuned, inducible two-plasmid CRISPR/Cas9-system for chromosomal gene insertion in L. plantarum. …”
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CRISPR/Cas9-Based Protocol for Precise Genome Editing in Induced Pluripotent Stem Cells by Avinash Singh, Swathy Babu, Marcus Phan, Shauna Yuan

“…The advent of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based genome editing has marked a significant advancement in genetic engineering technology. …”
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Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks by Chongwei Bi, Baolei Yuan, Yingzi Zhang, Mengge Wang, Yeteng Tian, Mo Li

“…Taken together, our study addresses a ubiquitous and overlooked risk of unintended LgIns in Cas9 editing, contributing valuable insights for the safe use of Cas9 editing tools.…”
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A CRISPR-Cas9 system protecting E. coli against acquisition of antibiotic resistance genes by Danna Lee, Petra Muir, Sara Lundberg, August Lundholm, Linus Sandegren, Sanna Koskiniemi

“…Therefore, we developed an engineered CRISPR-Cas9 system that protects bacteria from horizontal gene transfer. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

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New frontiers in CRISPR: Addressing antimicrobial resistance with Cas9, Cas12, Cas13, and Cas14 by Ahmed S.A. Ali Agha, Ali Al-Samydai, Talal Aburjai

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CRISPR-Cas9-mediated editing of ZmPL1 gene improves tolerance to drought stress in maize by Chunlai Wang, Yangyang Zhou, Yimeng Wang, Peng Jiao, Siyan Liu, Shuyang Guan, Yiyong Ma

Subjects: “…CRISPR-Cas9…”
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A hypomorphic mutation in the mouse Csn1s1 gene generated by CRISPR/Cas9 pronuclear microinjection by A. V. Smirnov, T. A. Shnaider, A. N. Korablev, A. M. Yunusova, I. A. Serova, N. R. Battulin

“…We performed pronuclear microinjections with Cas9 mRNA and sgRNA against the first coding exon of the mouse Csn1s1 gene to introduce random mutations in the α-casein (Csn1s1) signal peptide sequence at the beginning of the mouse gene. …”
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Improvement of the knock-in effciency in the genome of human induced pluripotent stem cells using the CRISPR/Cas9 system by М. М. Gridina

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Integrating malaria vaccine and CRISPR/Cas9 gene drive: a comprehensive strategy for accelerated malaria eradication by Israel Charles Abraham, John Ehi Aboje, Bonaventure Michael Ukoaka, Kehinde Tom-Ayegunle, Maryam Amjad, Anas Abdulkader, Chinonyelum Emmanuel Agbo, Oluwatosin Ayokunle Akinruli, Taiwo Rebecca Akisanmi, Emmanuel Oyedeji Oyetola, Gbolahan Olatunji, Emmanuel Kokori, Nicholas Aderinto

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Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in Plasmodium berghei by Samhita Das, Tanaya Unhale, Carine Marinach, Belsy del Carmen Valeriano Alegria, Camille Roux, Hélène Madry, Badreddine Mohand Oumoussa, Rogerio Amino, Shiroh Iwanaga, Sylvie Briquet, Olivier Silvie

“…Here, we developed new P. berghei parasite lines by integrating the DiCre cassette and a fluorescent marker in parasites constitutively expressing Cas9. Owing to the dual integration of CRISPR/Cas9 and DiCre, these new lines allow unparalleled levels of gene modification and conditional regulation simultaneously. …”
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Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporter by Yudong Teng, Kelsey Arbogast, Harald Junge, Zhe Chen

“…Summary: Here, we present a protocol to alter the production of alternatively spliced mRNA variants, without affecting the overall gene expression, through CRISPR-Cas9-engineered genomic mutations in mice. We describe steps for designing guide RNA to direct Cas9 endonuclease to consensus splice sites, producing transgenic mice through pronuclear injection, and screening for desired mutations in cultured mammalian cells using a minigene splicing reporter. …”
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Cellular Reprogramming, Genome Editing, and Alternative CRISPR Cas9 Technologies for Precise Gene Therapy of Duchenne Muscular Dystrophy by Peter Gee, Huaigeng Xu, Akitsu Hotta

“…In the past decade, the development of two innovative technologies, namely, induced pluripotent stem cells (iPSCs) and the CRISPR Cas9 system, has enabled researchers to model diseases derived from patient cells and precisely edit DNA sequences of interest, respectively. …”
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Generation of a USP9Y knockout human embryonic stem cell line with CRISPR-Cas9 technology by Sai Wei, Yuting Zhen, Chao Sun, Yanlin Ma, Qi Li, Luan Wen

“…We generated a USP9Y knockout hESC line using CRISPR-Cas9 in the male-derived H1 line. Targeted deletion of the USP9Y gene was confirmed via PCR and sequencing. …”
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Supersecondary Structure Code for RNA: Trace of Conformational Change on the Mycoplasma pneumoniae Ribosome and the R‑Loop Formation of Cas9 by Hiroshi Izumi, Laurence A. Nafie, Rina K. Dukor

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Efficient Dual Cas9 Nickase Correction of a Prevalent Pathogenic LAMB3 Variant for Junctional Epidermolysis Bullosa by Alex du Rand, John Hunt, Daniel Verdon, Ben Buttle, P. Rod Dunbar, Diana Purvis, Vaughan Feisst, Hilary Sheppard

Subjects: “…CRISPR/Cas9…”
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