Plasmodium falciparum transcription factor AP2-06B is mutated at high frequency in Southeast Asia but does not associate with drug resistance by Qiyang Shi, Changhong Wang, Wenluan Yang, Xiaoqin Ma, Jianxia Tang, Jiayao Zhang, Guoding Zhu, Yinlong Wang, Yaobao Liu, Xiaoqin He

“…To determine whether AP2-06B can alter the expression of pfk13 and pfcrt, as well as cause artemisinin and quinoline resistance in Plasmodium, we generated both a K3124R mutation and conditional knockdown of AP2-06B in Pf3D7 using CRISPR/Cas9-mediated genome editing.ResultsChIP-Seq analysis showed that AP2-06B can bind to the loci of the Plasmodium genes pfk13 and pfcrt. …”
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Novel role of FTO in regulation of gut–brain communication via Desulfovibrio fairfieldensis-produced hydrogen sulfide under arsenic exposure by Ruonan Chen, Xiaoqin Chai, Yunxiao Zhang, Tianxiu Zhou, Yinyin Xia, Xuejun Jiang, Bo Lv, Jun Zhang, Lixiao Zhou, Xin Tian, Ruonan Wang, Lejiao Mao, Feng Zhao, Hongyang Zhang, Jun Hu, Jingfu Qiu, Zhen Zou, Chengzhi Chen

“…Also, this administration resulted in the enhancement of m6A modification and the reduction of FTO expression in the intestine. By using both CRISPR/Cas9-based FTO knock-in strategy and adeno-associated virus (AAV)-mediated overexpression of FTO in the intestine, we established for the first time that up-regulation of FTO remarkably ameliorated arsenic-induced disruption of intestinal barriers and altered microenvironment of mice. …”
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KEAP1 mutations as key crucial prognostic biomarkers for resistance to KRAS-G12C inhibitors by Linyan Tian, Chengming Liu, Sufei Zheng, Huiyang Shi, Fang Wei, Wenxin Jiang, Yucheng Dong, Haiyan Xu, Enzhi Yin, Nan Sun, Jie He, Yan Wang

“…Methods This systematic meta-analysis synthesized studies available through September 2024 across PubMed, Cochrane Library, SpringerLink, and Embase. Using CRISPR/Cas9 technology, this study generated cells with KEAP1 and STK11 knockouts, and utilized lentiviral vectors to overexpress PD-L1. …”
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Revealing the biological features of the axolotl pancreas as a new research model by Hui Ma, Hui Ma, Hui Ma, Guangcong Peng, Yan Hu, Binbin Lu, Binbin Lu, Yiying Zheng, Yingxian Wu, Weimin Feng, Yu Shi, Xiangyu Pan, Li Song, Ina Stützer, Yanmei Liu, Jifeng Fei, Jifeng Fei, Jifeng Fei

“…To assess pancreatic gene function, Pdx1 mutants were generated using CRISPR/Cas9-mediated gene editing, and their effects on pancreatic morphology, endocrine cell populations, and glucose homeostasis were analyzed.ResultsThe axolotl pancreas contains all known pancreatic cell types and develops from dorsal and ventral buds. …”
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A Series of Novel Alleles of <i>Ehd2</i> Modulating Heading and Salt Tolerance in Rice by Peng Xu, Shulei Hao, Xiaoxia Wen, Guifang Ma, Qinqin Yang, Ling Liu, Galal Bakr Anis, Yingxin Zhang, Lianping Sun, Xihong Shen, Qunen Liu, Daibo Chen, Yongbo Hong, Yuyu Chen, Xiaodeng Zhan, Shihua Cheng, Liyong Cao, Weixun Wu

“…Functional validation through CRISPR/Cas9 knockout and complementation assays confirmed its role in regulating heading. …”
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Overcoming CD226-related immune evasion in acute myeloid leukemia with CD38 CAR-engineered NK cells by Luciana Melo Garcia, Achintyan Gangadharan, Pinaki Banerjee, Ye Li, Andy G.X. Zeng, Hind Rafei, Paul Lin, Bijender Kumar, Sunil Acharya, May Daher, Luis Muniz-Feliciano, Gary M. Deyter, Gabriel Dominguez, Jeong Min Park, Francia Reyes Silva, Ana Karen Nunez Cortes, Rafet Basar, Nadima Uprety, Mayra Shanley, Mecit Kaplan, Enli Liu, Elizabeth J. Shpall, Katayoun Rezvani

“…NK cells in patients with AML had lower expression of CD226. CRISPR-Cas9 deletion of CD226 led to reduced LFA-1 recruitment, poor synapse formation, and decreased NK cell anti-leukemic activity. …”
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MAM‐STAT3‐Driven Mitochondrial Ca+2 Upregulation Contributes to Immunosenescence in Type A Mandibuloacral Dysplasia Patients by Arshad Ahmed Padhiar, Xiaohong Yang, Syed Aqib Ali Zaidi, Zhu Li, Jinqi Liao, Wei Shu, Arif Ali Chishti, Liangge He, Gulzar Alam, Abdullah Faqeer, Ilyas Ali, Shuai Zhang, Ting Wang, Tao Liu, Meiling Zhou, Gang Wang, Yan Zhou, Guangqian Zhou

“…Inflammaging is mitigated by various FDA‐approved MAM‐STAT3 upstream inhibitors, such as (Tocilizumab) or by correcting R527C mutation with CRISPR/CAS9. These results provide new insights into MAD disease and propose targeting defective mitochondrial Ca+2 homeostasis as a promising therapy for reversing immunosenescence.…”
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Binding of LncDACH1 to dystrophin impairs the membrane trafficking of Nav1.5 protein and increases ventricular arrhythmia susceptibility by Genlong Xue, Jiming Yang, Yang Zhang, Ying Yang, Ruixin Zhang, Desheng Li, Tao Tian, Jialiang Li, Xiaofang Zhang, Changzhu Li, Xingda Li, Jiqin Yang, Kewei Shen, Yang Guo, Xuening Liu, Guohui Yang, Lina Xuan, Hongli Shan, Yanjie Lu, Yang Baofeng, Zhenwei Pan

“…Strikingly, activation of Dystrophin transcription by dCas9-SAM system in lncDach1-TG mice rescued the impaired membrane distribution of dystrophin and Nav1.5, and prevented the occurrence of ventricular arrhythmia. …”
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Dual α-globin-truncated erythropoietin receptor knockin restores hemoglobin production in α-thalassemia-derived erythroid cells by Simon N. Chu, Eric Soupene, Devesh Sharma, Roshani Sinha, Travis McCreary, Britney Hernandez, Huifeng Shen, Beeke Wienert, Chance Bowman, Han Yin, Benjamin J. Lesch, Kun Jia, Kathleen A. Romero, Zachary Kostamo, Yankai Zhang, Tammy Tran, Marco Cordero, Shota Homma, Jessica P. Hampton, James M. Gardner, Bruce R. Conklin, Tippi C. MacKenzie, Vivien A. Sheehan, Matthew H. Porteus, M. Kyle Cromer

“…To address this, we designed a Cas9/AAV6-mediated genome editing strategy that integrates a functional α-globin gene into the β-globin locus in α-thalassemia patient-derived hematopoietic stem and progenitor cells (HSPCs). …”
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GM-CSF and IL-21-armed oncolytic vaccinia virus significantly enhances anti-tumor activity and synergizes with anti-PD1 immunotherapy in pancreatic cancer by Yujing Xuan, Wenyi Yan, Ruimin Wang, Xibin Wang, Yu Guo, Huilin Dun, Ziyan Huan, Lihua Xu, Ruxia Han, Xianlei Sun, Lingling Si, Nicholas R. Lemoine, Nicholas R. Lemoine, Yaohe Wang, Yaohe Wang, Pengju Wang

“…In this study, we constructed a tumor-selective vaccinia virus (VV) with deletions of the TK and A49 genes (VVLΔTKΔA49, VVL-DD) using CRISPR-Cas9-based homologous recombination. VVL-DD exhibited significant tumor selectivity in vitro and anti-tumor potency in vivo in a murine pancreatic cancer model. …”
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A TRAILR2/CDH3 bispecific antibody demonstrates selective apoptosis and tumor regression in CDH3-positive pancreatic cancer by Peter Jung, Stefan P. Glaser, Jing Han, Alexandra Popa, Laura Pisarsky, Ningping Feng, Antonia Geyer, Franziska Haderk, Donat Alpar, Christopher Bristow, Susanne Schmittner, Paula-Elena Traexler, Mikhila Mahendra, Birgit Poehn, Poojabahen Gandhi, Roberto Fiorelli, Sanket Awate, Nicole Budano, Florian Martin, Christoph Albrecht, Barbara Drobits-Handl, Sathanandam S. Anand, Srinath Kasturirangan, Francesca Trapani, Norbert Schweifer, Joseph R. Marszalek, Ulrike Tontsch-Grunt, Mark Pearson, Timothy P. Heffernan, Norbert Kraut, Christopher P. Vellano, Juan Manuel García-Martínez

“…We demonstrate target-dependent TR2/CDH3 BAB anti-tumor activity in CRISPR/Cas9-engineered TRAILR2 or CDH3 knock-out cells. …”
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Placental growth factor modulates endothelial NO production and exacerbates experimental hepatopulmonary syndrome by Fabien Robert, Feriel Benchenouf, My Ngoc Ha, Alessandra Cuomo, Mina Ottaviani, Maxime Surbier, Raphaël Thuillet, Corinne Normand, Florent Dumont, Céline Verstuyft, Frederic Fiore, Frederic Guinut, Marc Humbert, Audrey Coilly, Emmanuel Gonzales, Olivier Sitbon, Ly Tu, Christophe Guignabert, Laurent Savale

“…Plgf-knockout (Plgf–/–) rats were generated using CRISPR-Cas9. Lung RNA-sequencing analysis was performed in the CBDL model. …”
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