Investigating a role for PUB17 and PUB16 in the self‐incompatibility signaling pathway in transgenic Arabidopsis thaliana by Paula K. S. Beronilla, Daphne R. Goring

“…Two close ARC1 homologs, PUB17 and PUB16, were selected, and (Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) technology was used to generate pub17 and pub16 mutations in the C24 accession. …”
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Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha by Senqin Hou, Shibin Yang, Wenqin Bai

“…However, the lack of precise and efficient genome editing tools severely hinders the construction of cell factories. Although the CARISP-Cas9 system has been established in Ogataea polymorpha, the gene editing efficiency, especially for multiple genes edition, needs to be further improved. …”
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TransLeish: Identification of membrane transporters essential for survival of intracellular Leishmania parasites in a systematic gene deletion screen by Andreia Albuquerque-Wendt, Ciaran McCoy, Rachel Neish, Ulrich Dobramysl, Çağla Alagöz, Tom Beneke, Sally A. Cowley, Kathryn Crouch, Richard J. Wheeler, Jeremy C. Mottram, Eva Gluenz

“…A total of 312 protein components of small molecule carriers, ion channels and pumps were identified and targeted in a CRISPR-Cas9 gene deletion screen in the promastigote form, yielding 188 viable null mutants. …”
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Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis by Guo-Ping Chen, Jian Yang, Guo-Feng Qian, Wei-Wei Xu, Xiao-Qin Zhang

“…In this study, mouse conditional knockout of VSMC GGTase-I (Pggt1bΔ/Δ mice) was generated using the CRISPR/Cas9 system. The mouse model of diabetes-accelerated atherosclerosis was induced by streptozotocin injections and an atherogenic diet. …”
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Aquatic Invertebrate Antimicrobial Peptides in the Fight Against Aquaculture Pathogens by Tomás Rodrigues, Francisco Antonio Guardiola, Daniela Almeida, Agostinho Antunes

“…These AMPs could serve as feed additives, therapeutic agents, or in genetic engineering approaches like CRISPR/Cas9-mediated transgenesis to enhance resilience of farmed species. …”
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Mice with NOP2/sun RNA methyltransferase 5 deficiency die before reaching puberty due to fatal kidney damage by Hongya Zhang, Xiaohui Li, Jing Bai, Cong Zhang

“…Therefore, in this study, we investigate the role of NSUN5 in mice.Methods In the present research, we built a mouse model (Nsun5-/-) using the CRISPR/Cas9 system to investigated the specific role of NSUN5.Results We observed that Nsun5-/- mice had a reduced body weight compared to wild-type mice. …”
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Emerging Strategies in Cartilage Repair and Joint Preservation by Mircea Adrian Focsa, Sorin Florescu, Armand Gogulescu

“…Gene-editing tools like CRISPR/Cas9 have facilitated targeted cellular modifications, while novel biomaterials such as hydrogels, biodegradable scaffolds, and 3D-printed constructs have improved mechanical support and tissue integration. …”
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Shared rare genetic variants in multiplex autism families suggest a social memory gene under selection by Kang Seon Lee, Taeyeop Lee, Mujun Kim, Elizaveta Ignatova, Hyo-Jeong Ban, Min Kyung Sung, Younghoon Kim, Youn-Jae Kim, Jin-Hee Han, Jung Kyoon Choi

“…One variant in this family was 6 bp away from human-specific trinucleotide fixation. Additionally, CRISPR/Cas9 experiments demonstrated downregulation by a family variant and upregulation by a fixed site. …”
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Therapeutic reduction of neurocan in murine intracerebral hemorrhage lesions promotes oligodendrogenesis and functional recovery by Hongmin Li, Samira Ghorbani, Olayinka Oladosu, Ping Zhang, Frank Visser, Jeff Dunn, Yunyan Zhang, Chang-Chun Ling, V. Wee Yong, Mengzhou Xue

“…Methods Mice were retro-orbitally injected with adeno-associated virus (AAV-CRISPR/Cas9) to reduce neurocan deposition after ICH induction by collagenase. …”
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Heterozygous pathogenic STT3A variation leads to dominant congenital glycosylation disorders and functional validation in zebrafish by Linxue Meng, Zhixu Fang, Li Jiang, Yinglan Zheng, Siqi Hong, Yu Deng, Lingling Xie

“…We constructed wild-type and variant plasmids, transfected them into HEK293T cells and detected the expression levels of the STT3A protein. We performed CRISPR-Cas9 to establish heterozygous knockdown zebrafish to validate the functional implications of autosomal dominant inheritance of STT3A in pathogenesis. …”
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Cytotoxic mechanisms of pemetrexed and HDAC inhibition in non-small cell lung cancer cells involving ribonucleotides in DNA by Tobias Solli Iveland, Lars Hagen, Mirta Mittelstedt Leal de Sousa, Nina Beate Liabakk, Per Arne Aas, Animesh Sharma, Bodil Kavli, Geir Slupphaug

“…Both HeLa (p53 negative) and A549 (p53 positive) showed robust activation of γH2AX upon treatment with this combination. Importantly, CRISPR/Cas9 knockout of the uracil-DNA glycosylase UNG did not affect γH2AX activation or sensitivity to pemetrexed. …”
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Generation and characterization of the LINC01405 knockout human embryonic stem cell line by Yaping Xu, Hongchun Wu, Jingxiu Jiang, Lingqun Ye, Kaili Hao, Kunjun Han, Shijun Hu, Wei Lei, Zhikun Guo

“…By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. …”
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SLC7A5 is required for cancer cell growth under arginine-limited conditions by Kyle N. Dunlap, Austin Bender, Alexis Bowles, Alex J. Bott, Joshua Tay, Allie H. Grossmann, Jared Rutter, Gregory S. Ducker

“…We performed a metabolism-focused functional screen using CRISPR-Cas9 to identify pathways and factors that enable tumor growth in an arginine-depleted environment. …”
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Plasmid-free production of the plant lignan pinoresinol in growing Escherichia coli cells by U. Joost Luelf, Alexander Wassing, Lisa M. Böhmer, Vlada B. Urlacher

“…To this end, a simple and versatile plasmid toolbox for CRISPR/Cas9-assisted chromosomal integration has been developed, which allows the easy transfer of genes from the pET vector series into the E. coli chromosome. …”
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Endoplasmic reticulum stress-related super enhancer promotes epithelial-mesenchymal transformation in hepatocellular carcinoma through CREB5 mediated activation of TNC by Anqi Wang, Sitong Yan, Jiatao Liu, Xiang Chen, Mengyao Hu, Xiao Du, Weijia Jiang, Zhipeng Pan, Lulu Fan, Guoping Sun

“…In this study, we identified 17 ERS-related SEs by comparing ERS-HCC cells with untreated control cells using ChIP-seq and RNA-seq. CRISPR-Cas9 and RT-qPCR identified CAMP responsive element binding protein 5 (CREB5) as a key target of ERS-related SE. …”
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Gene-editing in patient and humanized-mice primary muscle stem cells rescues dysferlin expression in dysferlin-deficient muscular dystrophy by Helena Escobar, Silvia Di Francescantonio, Julia Smirnova, Robin Graf, Stefanie Müthel, Andreas Marg, Alexej Zhogov, Supriya Krishna, Eric Metzler, Mina Petkova, Oliver Daumke, Ralf Kühn, Simone Spuler

“…We targeted a frequent loss-of-function, DYSF exon 44, founder frameshift mutation with mRNA-mediated delivery of SpCas9 in combination with a mutation-specific sgRNA to primary muscle stem cells from two homozygous patients. …”
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Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice by Xiaolong Fu, Linqing Zhang, Yecheng Jin, Xiaoyang Sun, Aizhen Zhang, Zongzhuang Wen, Yichen Zhou, Ming Xia, Jiangang Gao

“…In the present study, we used CRISPR/Cas9 technology to establish a Myh14 knockout mice line in CBA/CaJ background (now referred to as Myh14−/− mice) and clarify the role of MYH14 in the cochlea and NIHL. …”
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NLRP3 Inflammasome Simultaneously Involved in Autophagy and Phagocytosis of THP-1 Cells to Clear Aged Erythrocytes by Qin Li, Fengyong Zhao, Jiamin Zhang, Ying Yang, Zhonghui Guo, Chen Wang, Qixiu Yang, Ying Sun, Ziyan Zhu

“…We constructed a stable and low-NLRP3 expression THP-1 cell line using CRISPR/Cas9 technology. The analysis of erythrocyte clearance and autophagy of THP-1 cells with low NLRP3 expression showed that autophagy changes together when THP-1 engulfs aged RBCs. …”
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Long-term correction of hemophilia A via integration of a functionally enhanced FVIII gene into the AAVS1 locus by nickase in patient-derived iPSCs by Do-Hun Kim, Sang-Hwi Choi, Jin Jea Sung, Sieun Kim, Hanui Yi, Sanghyun Park, Chan Wook Park, Young Woo Oh, Jungil Lee, Dae-Sung Kim, Jong-Hoon Kim, Chul-Yong Park, Dong-Wook Kim

“…We inserted FE-FVIII with a human elongation factor-1 alpha (EF1α) promoter into the AAVS1 locus of HA patient-derived iPSCs via CRISPR/Cas9 (D10A) nickase to ensure expression in any cell type. …”
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RNA-binding protein HuR reprograms immune T cells and promotes oral squamous cell carcinoma by Mrinmoyee Majumder, Harinarayanan Janakiraman, Paramita Chakraborty, Anitha Vijayakumar, Sari Mayhue, Hong Yu, Toros Dincman, Romeo Martin, Elizabeth O'Quinn, Shikhar Mehrotra, Viswanathan Palanisamy

“…We examined HuR's effects on oral malignancies and immune cell function in vitro and in vivo using oral carcinoma cells and transgenic HuR knockout (KO) mice. CRISPR/Cas9-mediated HuR deletion in mice syngeneic oral cancer cells eliminated colony formation and tumor development. …”
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