Harnessing CRISPR interference to resensitize laboratory strains and clinical isolates to last resort antibiotics by Angelica Frusteri Chiacchiera, Michela Casanova, Massimo Bellato, Aurora Piazza, Roberta Migliavacca, Gregory Batt, Paolo Magni, Lorenzo Pasotti

“…Here, we evaluated an antibiotic re-sensitization strategy based on CRISPR interference (CRISPRi), which inhibits gene expression without damaging target DNA. …”
Get full text

dCas12a-mediated CRISPR interference for multiplex gene repression in cyanobacteria for enhanced isobutanol and 3-methyl-1-butanol production by Hao Xie, Barbara Bourgade, Karin Stensjö, Peter Lindblad

Subjects: Get full text

CRISPRi knockdown of mycobacterial tkt gene potentiates the anti-mycobacterial activity of phyto-compounds from selected medicinal plants by Mashilo Mash Matotoka, Gabriel Tshwahla Mashabela, Peter Masoko

Subjects: Get full text

CRISPRi screening reveals E. coli’s anaerobic-like respiratory adaptations to gentamicin: membrane depolarization by CpxR by Donghui Choe, Eunju Lee, Yoseb Song, Sun Chang Kim, Ki Jun Jeong, Bernhard Palsson, Byung-Kwan Cho, Suhyung Cho

Subjects: “…CRISPR interference…”
Get full text

CEBPD regulates CD47 and MAP4K4 via chromatin accessibility in canine mammary tumor monocytes by Jeong-Woon Lee, Dabin Lee, Kyung-Ju Shin, Keun Hong Son, Je-Yoel Cho

Subjects: Get full text

Modular and signal-responsive transcriptional regulation using CRISPRi-aided genetic switches in Escherichia coli by Seong Keun Kim, Seung-Gyun Woo, Jun-Hong Park, Seung-Goo Lee, Dae-Hee Lee

Subjects: “…CRISPR interference…”
Get full text

A method for high‐throughput production of sequence‐verified DNA libraries and strain collections by Justin D Smith, Ulrich Schlecht, Weihong Xu, Sundari Suresh, Joe Horecka, Michael J Proctor, Raeka S Aiyar, Richard A O Bennett, Angela Chu, Yong Fuga Li, Kevin Roy, Ronald W Davis, Lars M Steinmetz, Richard W Hyman, Sasha F Levy, Robert P St.Onge

Subjects: Get full text

Precise measurement of molecular phenotypes with barcode-based CRISPRi systems by Joseph H. Lobel, Nicholas T. Ingolia

“…Here, we realize these goals by optimizing CRISPR interference with barcoded expression reporter sequencing (CiBER-seq) to dramatically improve the sensitivity and scope of genome-wide screens. …”
Get full text

CRISPR-based functional genomics tools in vertebrate models by Gaurav K. Varshney, Shawn M. Burgess

“…In addition to gene editing, transcriptional modulation, that is, CRISPR interference and CRISPR activation systems, can elucidate the mechanisms of gene regulation. …”
Get full text

Genomic engineering in Rhizobium etli: implementation and evaluation of systems based on dCas9 by Oussama Bellahsen, Rafael Díaz-Méndez, David Romero

“…In this study, we developed a robust CRISPR interference (CRISPRi) system to precisely modulate gene expression in the bacterium Rhizobium etli, the nitrogen-fixing symbiont of the common bean. …”
Get full text

Engineering intercellular communication using M13 phagemid and CRISPR-based gene regulation for multicellular computing in Escherichia coli by Hadiastri Kusumawardhani, Florian Zoppi, Roberto Avendaño, Yolanda Schaerli

“…Once inside the receiver cells, the transferred sgRNAs mediate gene regulation via CRISPR interference. Leveraging this approach, we successfully constructed one-, two-, and four-input logic gates. …”
Get full text

Chemical genomics informs antibiotic and essential gene function in Acinetobacter baumannii. by Jennifer Suzanne Tran, Ryan David Ward, Rubén Iruegas-López, Ingo Ebersberger, Jason Matthew Peters

“…Here, we systematically probe the function of essential genes by screening a CRISPR interference knockdown library against a diverse panel of chemical inhibitors, including antibiotics. …”
Get full text

Identification of replicative aging and inflammatory aging signatures via whole-genome CRISPRi screens by Lingling Wu, Xiang Zhu, Yanxia Liu, Dehua Zhao, Betty Chentzu Yu, Zheng Wei, Xueqiu Lin, Lei S. Qi

“…Results We perform multiple genome-wide CRISPR interference (CRISPRi) screenings in human primary mesenchymal stem cells derived from adipose tissue during either replicative senescence or inflammation-induced senescence. …”
Get full text

Genome-wide analysis of fitness determinants of Staphylococcus aureus during growth in milk. by Marita Torrissen Mårli, Anne Olsdatter Ohren Nordraak, Vincent de Bakker, Anja Ruud Winther, Xue Liu, Jan-Willem Veening, Davide Porcellato, Morten Kjos

“…In this study, we conducted a genome-wide CRISPR interference sequencing (CRISPRi-seq) screen with the laboratory strain S. aureus NCTC8325-4, to identify fitness determinants essential for S. aureus growth and survival in milk. …”
Get full text

A rapid and efficient strategy for combinatorial repression of multiple genes in Escherichia coli by Yi Zheng, Yuxia Mo, Yingbo Yuan, Tianyuan Su, Qingsheng Qi

“…Abstract Background The regulation of multiple gene expression is pivotal for metabolic engineering. Although CRISPR interference (CRISPRi) has been extensively utilized for multi-gene regulation, the construction of numerous single-guide RNA (sgRNA) expression plasmids for combinatorial regulation remains a significant challenge. …”
Get full text

PerturbNet predicts single-cell responses to unseen chemical and genetic perturbations by Hengshi Yu, Weizhou Qian, Yuxuan Song, Joshua D Welch

“…Moreover, PerturbNet accurately predicts the distribution of single-cell gene expression states following CRISPR activation or CRISPR interference by leveraging gene functional annotations. …”
Get full text

Mycobacterium tuberculosis requires SufT for Fe-S cluster maturation, metabolism, and survival in vivo. by Ashutosh Tripathi, Kushi Anand, Mayashree Das, Ruchika Annie O'Niel, Sabarinath P S, Chandrani Thakur, Raghunatha Reddy R L, Raju S Rajmani, Nagasuma Chandra, Sunil Laxman, Amit Singh

“…We efficiently repressed the expression of SufT to generate a SufT knock-down strain in Mtb (SufT-KD) using CRISPR interference. Depleting SufT reduces aconitase's enzymatic activity under standard growth conditions and in response to oxidative stress and iron limitation. …”
Get full text

Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens by Alicia Calvo-Villamañán, Jorge Sastre-Dominguez, Álvaro Barrera-Martín, Coloma Costas, Álvaro San Millan

“…In this study, we introduce plasmid-wide CRISPR interference (CRISPRi) screens as a tool to dissect plasmid-associated fitness effects. …”
Get full text

Single cell variability of CRISPR‐Cas interference and adaptation by Rebecca E McKenzie, Emma M Keizer, Jochem N A Vink, Jasper van Lopik, Ferhat Büke, Vera Kalkman, Christian Fleck, Sander J Tans, Stan J J Brouns

“…We observed that CRISPR interference is fast with a narrow distribution of clearance times. …”
Get full text

Novel regulators of PrPC biosynthesis revealed by genome-wide RNA interference. by Daniel Heinzer, Merve Avar, Daniel Patrick Pease, Ashutosh Dhingra, Jiang-An Yin, Elke Schaper, Berre Doğançay, Marc Emmenegger, Anna Spinelli, Kevin Maggi, Andra Chincisan, Simon Mead, Simone Hornemann, Peter Heutink, Adriano Aguzzi

“…Recursive candidate attrition through multiple secondary screens yielded 54 novel regulators of PrPC, 9 of which were confirmed by CRISPR interference as robust regulators of PrPC biosynthesis and degradation. …”
Get full text