激素敏感型肾病综合征儿童外周血T、B淋巴细胞亚群的研究 by 蒋小云

“…B淋巴细胞亚群数量,同时以16例正常儿童作为对照组进行相同检测?【结果】 SSNS患儿疾病活动期外周血CD3+CD8+T细胞低于对照组,CD4/CD8比值上升,P < 0.05;CD3+T?CD3+CD4+T?…”
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Clinical Significance of Negative Costimulatory Molecule PD-1/PD-L1 on Peripheral Blood Regulatory T Cell Levels among Patients with Pulmonary Tuberculosis by Chenchen Yang, Fanchao Dai, Rexiti Pulati, Yan Jiao, Qian Xu

“…This study aimed to investigate the expression and clinical significance of negative costimulatory molecules programmed death-1 (PD-1) and programmed death ligand 1 (PD-L1) on CD4+CD25+CD127low regulatory T cells (Tregs) in peripheral blood of patients with active pulmonary tuberculosis (TB). …”
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Lymphocyte Subpopulations in Pulmonary Tuberculosis Patients by Figen Deveci, H. Handan Akbulut, Ilhami Celik, M. Hamdi Muz, Fulya İlhan

“…Protection against Mycobacterium tuberculosis is based on cell-mediated immunity, most importantly involving CD4+ and CD8+ T-cell subsets. The aim of this study was to evaluate CD4+ and CD8+ T-cell profiles and CD19+ and CD3-CD(16+56)+ populations in patients with pulmonary tuberculosis. …”
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Evaluation of T memory cells in knee osteoarthritis patients by Rehab S. Mohamed, Alaa M. Hashim, Abdelhafeez M. Moshrif, Ali Sobhy

“…All the included patients were subjected to radiographic examination, Western Ontario and McMaster Universities score, Kellgren–Lawrence grade, and laboratory investigations (complete blood count–C-responsive protein–erythrocyte sedimentation rate) and assessment of T memory cells by flow cytometry. Results The mean CD3+CD4+CD8− T cells level, CD3+CD4+CD45Ro+ memory T cells level, CD3+CD8+CD4− T level, and CD3+CD8+CD45RO+ memory T was lower in OA cases (4.8±3.1) in comparison with controls. …”
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The magnitude of exercise‐induced progenitor cell mobilisation and extravasation is positively associated with cardiorespiratory fitness by Mark Ross, Sarah Aldred, Mark T. Drayson, Jos A. Bosch, James E. Turner

“…Blood samples were collected before exercise (Pre), in the final minute of exercise (0 h) and afterwards at 0.25, 1 and 24 h. Pan‐progenitor cells (CD34+, CD34+CD45dim) and putative endothelial progenitor cells (CD34+CD133+, CD34+VEGFR2+, CD34+CD45dimVEGFR2+) were quantified using flow cytometry. …”
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Mechanisms of Stress Alleviation after Lime and Biochar Applications for Brassica napus L. in Cadmium-Contaminated Soil by Ming Lei, Zhuoqing Li, Beibei Zhang, Xinqi Wang, Boqing Tie, Tehreem Ayaz, Xia Lu

“…Lime and biochar amendments are widely used to immobilize cadmium (Cd) in agricultural soils and to ensure food security. …”
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Study of the Physiological Dynamics of Cadmium Accumulation in Two Varieties of Rice with Different Cadmium-Accumulating Properties by Shangdu Zhang, Xiang Wu, Ju Peng, Xiufei Meng, Bangzhi Shi, Leliang Zhou, Lianyang Bai

“…This study focused on cadmium (Cd) uptake by two rice varieties, Yuzhenxiang (YZX) and Xiangwanxian 12 (XWX), which differ in their capacity to accumulate Cd, i.e., XWX > YZX. …”
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Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood by Valentina Tirelli, Barbara Ghinassi, Anna Rita Migliaccio, Carolyn Whitsett, Francesca Masiello, Massimo Sanchez, Giovanni Migliaccio

“…In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the corresponding CD34pos cells, suggesting that MNC may also contain CD34neg HPC. …”
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Effect of chemoradiotherapy on the dynamics of circulating lymphocyte subsets in patients with non-metastatic nasopharyngeal carcinoma by Lilan Yi, Lilan Yi, Yinfang Gu, Yinfang Gu, Longhua Guo, Longhua Guo, Xiaofang Zou, Xiaofang Zou, Guowu Wu, Guowu Wu

“…Flow cytometry was employed to detect the expression of CD3, CD4, CD8, CD56, and CD19 on peripheral blood cells. …”
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100% oxygen mobilizes stem cells and up-regulates MIF and APRIL in humans: a new point on the hormetic dose curve by Kent J. MacLaughlin, Gregory P. Barton, Julia E. MacLaughlin, Jacob J. Lamers, Matthew D. Marcou, Matthew J. O’Brien, Rudolf K. Braun, Marlowe W. Eldridge

“…Conversely CD45−/CD34+/CD133+, CD45-/CD34+/CD133− and CD45−/CD34−/CD133+ phenotypes were downregulated suggesting differentiation into more mature phenotypes. …”
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Renca 条件培养基转化的调节性T细胞 对小鼠同种免疫的影响

“…【目的】 探讨小鼠肾癌细胞株Renca来源的条件培养基能否诱导CD4+ CD25- T细胞转化为具有免疫抑制效能的CD4+ CD25+ Foxp3+ T细胞65377; 【方法】 培养Renca提取上清获得条件培养基,将条件培养基与普通培养液按不同比例混合培养小鼠CD4+ CD25- T细胞7 d,以流式细胞仪检测CD4+ CD25+ T细胞和Foxp3+ 细胞的比例;再次分选CD4+ CD25+ T细胞检测其抑制细胞增殖的能力,体内过继性输入观察对移植物存活时间65380;局部排斥反应和迟发性超敏反应的影响65377;【结果】 与对照组相比,混合培养基显著增加培养体系中CD4+ CD25+ Foxp3+ T细胞的比例(P < 0.05),在一定范围内(≤75%)呈比例依赖关系;随转化性CD4+ CD25+ T细胞比例增加效应T细胞增殖能力减弱;体内输注显著延长移植物的存活时间(P < 0.05),达(29.6 ± 1.4) d,组织学观察显示能明显抑制局部排斥反应65377;【结论】 Renca条件培养基能诱导CD4+ CD25- 细胞转化为 CD4+ CD25+ Foxp3+ T细胞,被转化细胞在体内和体外均能发挥免疫抑制作用65377;…”
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间充质干细胞对B细胞增殖及免疫调节功能的影响 by 刘秋莉, 肖崇珺, 黄牡丹, 黄里, 陈小湧, 郑海清

“…获取健康供者外周血单个核细胞，并通过流式细胞仪分选出CD19+B细胞，进行荧光染料CFSE标记，然后分成单独培养组（CD19+B细胞）和共培养组（按CD19+B∶MSC：=5∶1的比例共培养），两组均采用CpG+CD40L刺激，96h后检测B细胞的增殖情况，并通过流式细胞荧光分选技术（FACS）检测CD19+CD5+B细胞的百分比及其分泌的IL-10水平；进一步观察单独培养组和共培养组的CD19+B细胞对CD4+T细胞增殖以及分泌IFN-γ的影响，最后加入IL-10中和抗体，再次验证B细胞对CD4+T细胞增殖以及分泌IFN-γ的影响。…”
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Analysis of Lymphocyte Subpopulations and Cytokines in COVID-19-Associated Pneumonia and Community-Acquired Pneumonia by Guohong Liu, Xianghu Jiang, Xiaojiao Zeng, Yunbao Pan, Haibo Xu

“…CD16+CD56+%, CD4+/CD8+ratio, CD19+, and CD3+CD4+ were identified as predictors of COVID-19 diagnosis by logistic regression. …”
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成人骨髓间质干细胞基本生物学特性 by 张丽蓉, 李　艳

“…L-DMEM 培养基有利于细胞的培养扩增。细胞流式细胞仪检测结果显示CD29、CD44 、CD59 、CD105 、CD166 表达阳 性, CD11a 、CD14、CD33、CD34、CD45、CD38 、CD80 、CD86 、CD117 表达为阴性。…”
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The Immunohistochemistry Profile of Lymphocytic Gastritis in Celiac Disease and Helicobacter Pylori Infection: Interplay between Infection and Inflammation by Efrat Broide, Judith Sandbank, Eitan Scapa, Nimrod Alain Kimchi, Michael Shapiro, Aaron Lerner

“…Lymphocytic gastritis (LG) is associated with helicobacter pylori (Hp) and celiac disease (CD). We aimed to clarify the relationship between Hp infection and CD by defining a unique histopathology profile of LG in these two diseases. …”
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Detection of serum soluble markers of immune activation in rheumatoid arthritis by T. Kogure, T. Itoh, Y. Shimada, T. Shintani, H. Ochiai, K. Terasawa

“…The mutual correlation among soluble CD4 (sCD4), soluble CD8 (sCD8), and soluble CD23 (sCD23) has not yet been studied in patients with rheumatoid arthritis (RA), although previous studies have demonstrated that certain soluble markers of immune activation are elevated in RA. …”
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肝癌患者肝移植前外周血免疫表型分析 by 邓小红, 张倩倩, 欧阳涓, 陈茂根, 唐秀鑫, 何晓顺, 黄俊琪

“…【结果】与健康对照组比较，肝癌肝移植受者组患者外周血CD4 + PD-1 + T细胞、调节T细胞 （CD4 + CD25 + CD39 + ）、B细胞、浆母细胞（CD27 high CD38 high IgD - IgM - ）、经典型单核细胞（CD14 high CD16 - ）及未成熟NK 细胞（CD3 - CD56 high ）均升高，差异具有统计学意义（P<0.05）；边缘区 B 细胞（CD27 + IgD + ）、非类型转换 B 细胞 （CD27 + CD38 dim IgM + ）、中间型单核细胞（CD14 high CD16 + ）及NK细胞（CD3 - CD56 + ）比例降低，差异具有统计学意义 （P<0.05）；其余细胞比例未见明显差异。…”
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A Circulating Subpopulation of Monocytic Myeloid-Derived Suppressor Cells as an Independent Prognostic/Predictive Factor in Untreated Non-Small Lung Cancer Patients by Eleni-Kyriaki Vetsika, Filippos Koinis, Marianthi Gioulbasani, Despoina Aggouraki, Anna Koutoulaki, Eirini Skalidaki, Dimitris Mavroudis, Vassilis Georgoulias, Athanasios Kotsakis

“…The percentages and correlation between MDSCs and distinct immune cells in the peripheral blood of 110 chemotherapy-naive patients before treatment and healthy controls were investigated using flow cytometry. Two monocytic [CD14+CD15−CD11b+CD33+HLA-DR−Lin− and CD14+CD15+CD11b+CD33+HLA-DR−Lin−] and a granulocytic [CD14−CD15+CD11b+CD33+HLA-DR−Lin−] subpopulations of MDSCs were identified, expressing inducible nitric oxide synthase, and reactive oxygen species, respectively. …”
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Correlation between Peripheral T Cell Subsets and the Activity of Thyroid-Associated Ophthalmopathy by Hong Hu, Liang Liang, Qian Ge, Xing Jiang, Zhizheng Fu, Chun Liu, Jian Long

“…The absolute number and percentage of total lymphocytes, CD3+T cells, CD4+T cells, CD8+ T cells, CD3+CD4-CD8-T cells (DNT cells), and CD3+CD4+CD8+ T cells (DPT cells) in peripheral blood were measured by flow cytometer. …”
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Clinical Factors of Blood Transfusion-Related Acute Lung Injury and Changes in Levels of Treg-Related Cytokines by Lifang Sun, Yu Liu

“…CD4 + CD25 + Treg was positively correlated with levels of IL-10 and TGF-β (P<0.05). …”
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