Targeting S100A9 attenuates social dysfunction by modulating neuroinflammation and myelination in a mouse model of autism by Hong Gong, Yao Lu, Shi-Long Deng, Ke-Yi Lv, Jing Luo, Yi Luo, Zhu-Lin Du, Ling-Feng Wu, Tian-Yao Liu, Xia-Qing Wang, Jing-Hui Zhao, Lian Wang, Mei-Ling Xia, Dong-Mei Zhu, Li-Wei Wang, Xiao-Tang Fan

“…BTBR mice displayed decreased social communication and increased repetitive behaviors compared to C57BL/6 mice. Interestingly, the above social dysfunction was attenuated by a pharmacological inhibitor of S100A9, accompanied by a significant reduction in the activated microglia morphological phenotype, inflammatory receptors, and proinflammatory cytokines. …”
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Rapid assessment of the osteogenic capacity of hydroxyapatite/aragonite using a murine tibial periosteal ossification model by Emma Steijvers, Yunshong Shi, Hong Lu, Weixin Zhang, Yitian Zhang, Feihu Zhao, Baichuan Wang, Louise Hughes, Jake E. Barralet, Giulia Degli-Alessandrini, Igor Kraev, Richard Johnston, Zengwu Shao, Frank H. Ebetino, James T. Triffitt, R. Graham G. Russell, Davide Deganello, Xu Cao, Zhidao Xia

“…A novel hydroxyapatite/aragonite (HAA) biomaterial was implanted into C57BL/6 mice juxtaskeletally between the tibia and tibialis anterior muscle. …”
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Effect of Tiaoxin Recipe on Memory Learning and Synaptic Plasticity in APP/PS1 Double Transgenic Alzheimer's Disease Mice based on αCaMKⅡ-CREB-BDNF Signal Pathway by Xiaowen WANG, Hong QIAN, Chunjuan SHEN, Yanjun LI, Haixin YUAN, Jian WANG

“…To explore the mechanism of Tiaoxin recipe in improving memory learning and synaptic plasticity in APP/PS1 double transgenic AD mice from the point of view of αCaMKⅡ-CREB-BDNF signal pathway.Methods:A total of 54 3-month-old APP/PS1 male mice were randomly divided into the model group, the Tiaoxin recipe group and the Donepezil group according to the method of random number table, with 18 cases in each group. Another 18 C57/BL6 male wild type mice of the same age and the same strain were set as the normal group. …”
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Correlation of the Changes of Gray Matter Volume with Aβ Deposition and Learning and Memory Abilities in APP/PS1 Mice at Different Periods by Yuhao ZHANG, Hao JIN, Xiufeng ZHANG, Shengxiang LIANG, Weilin LIU, Jing TAO, Lidian CHEN

“…Objective:To analyze the correlation in the changes of cerebral gray matter volume and the ability of memory learning, and the deposition of β-amyloid protein (Aβ) in related brain regions of Alzheimer's disease (AD) double-transgenic APP/PS1 model mice, which is expected to provide reliable behavioral and imaging basis for probing the pathological features of AD animal models.Methods:A total of two groups mice were included in this study, including the wild type (WT) group and the APP/PS1 group, with 36 mice in each group. The C57-BL/6 mice were selected in the WT group, and APP/PS1 double transgenic mice were selected in the APP/PS1 group. …”
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Pomacea canaliculata hemocyanin as a novel natural immunostimulant in mammals by Ignacio Rafael Chiumiento, Ignacio Rafael Chiumiento, María Alejandra Tricerri, María Alejandra Tricerri, María Fernanda Cortéz, Santiago Ituarte, Santiago Ituarte, Julia Tau, Karina Valeria Mariño, Paola Lorena Smaldini, Horacio Heras, Horacio Heras, Marcos Sebastián Dreon, Marcos Sebastián Dreon

“…This species, known for its high reproductive rate and easy rearing, represents a promising source of potential biomedical compounds, including hemocyanin.MethodsEmploying flow cytometry, fluorescence microscopy, immunoassays, and quantitative PCR, we analysed the effects of PcH on THP-1 monocytes and their derived macrophages, as well as its ability to induce humoral response on C57BL/6 mice. Additionally, we evaluated the structural stability of PcH across a wide range of temperature and pH values.Results and discussionOur findings demonstrate that PcH is a structurally stable protein that not only triggers a pro-inflammatory effect on THP-1 derived-macrophages by increasing IL1-β and TNF-α levels, but also promotes phenotypic changes associated with the monocyte-to-macrophage differentiation. …”
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Significance of changes in levels of neutrophil extracellular traps during development of silicosis in mice by Lu HAN, Ruru CHEN, Yulu XIONG, Ziye GUO, Hailan HE, Xiaohui HAO, Heliang LIU, Lingli GUO

“…MethodsA total of 28 SPF male C57BL/6J mice were randomly divided into a control group, and a model group, and the model group was subdivided into, a 2 d model group, a 7 d model group, and a 28 d model group, with 7 mice in each group. …”
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Effect and Mechanism of Swimming Exercise Preconditioning on Neurological Function of Mice with Intracerebral Hemorrhage by LI Yongxu, LIN Libin, LU Taotao, WEI Wei, LIN Zhicheng, XUE Xiehua

“…ObjectiveTo observe the effect of swimming exercise preconditioning on neurological function and expression levels of brain derived neurotrophic factor (BDNF), serine-threonine kinase (Akt) and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) proteins in perihematoma tissues of mice with intracerebral hemorrhage (ICH).MethodsA total of 60 male C57BL/6 mice were randomly divided into sham group, ICH 7 d group, SEP+ICH 7 d group (preconditioning group 1), ICH 14 d group and SEP+ICH 14 d group (preconditioning group 2), with 12 cases in each group. …”
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LRP11 promotes stem-like T cells via MAPK13-mediated TCF1 phosphorylation, enhancing anti-PD1 immunotherapy by Zhishui Chen, Gang Chen, Li Chen, Xiangli Zhao, Lingjuan Sun, Zhibo Ma, Xiaosheng Tan, Yuhao Tu, Jingzeng Wang, Peixiang Lan

“…In an in vivo setting, we employed male C57BL/6J injected with B16 cells or MC38 cells to build a tumor model to assess the effects of LDL and LRP11 inhibitors, LRP11 activators, MAPK13 inhibitors on tumor growth. …”
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Study on the improvement of behavior and pain and the mechanism of Yangpafang in subacute Parkinson's disease model mice by SHI Ying, MEI Shan‐shan, WANG Yuan‐ling, XIA A‐long, WANG Xiao‐wei, LI Jun

“…Methods Total 42 specific pathogen⁃free (SPF) C57BL/6J mice were randomly divided into control group, model group, Yangpafang low⁃ dose group [13.50 g/(kg·d)], medium ⁃dose group [40.50 g/(kg·d)] and high⁃dose group [121.50 g/(kg·d)], levodopa and benserazide group (7 mice in each group). …”
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Protective Mechanism of Alisol A 24-Acetate on Brain Microvascular Endothelial Cells in Mice with Cerebral Ischemia-Reperfusion Injury by TRPM2/Akt/GSK3β Pathway by LI Huihong, DENG Yunfei, WEI Wei, ZHAN Zengtu, LIN Libin, XUE Xiehua

“…ObjectiveTo explore the mechanism of Alisol A 24-acetate (24A) on improving brain microvascular endothelial cells (BMECs) injury after cerebral ischemia reperfusion in mice.MethodsA total of 45 10-week-old male C57BL/6 mice were randomly divided into sham surgery group, model group and 24A group, with 15 cases in each group. …”
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Repeated low-intensity noise exposure exacerbates age-related hearing loss via RAGE signaling pathway by Jianbin Sun, Na Sai, Tong Zhang, Chaoying Tang, Shuhang Fan, Qin Wang, Da Liu, Xianhai Zeng, Juanjuan Li, Weiwei Guo, Shiming Yang, Weiju Han

“…This study was conducted to further investigate the manner in which repeated low-intensity noise exposure contributed to hearing damage. Two-month-old C57BL/6 J mice were exposed to white noise at 96 dB SPL for 8 h per day over 7 days to induce TTS. …”
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CD40-TRAF6 inhibition suppresses cardiovascular inflammation, oxidative stress and functional complications in a mouse model of arterial hypertension by Lea Strohm, Henning Ubbens, Dominika Mihalikova, Alexander Czarnowski, Paul Stamm, Michael Molitor, Stefanie Finger, Matthias Oelze, Dorothee Atzler, Philip Wenzel, Philipp Lurz, Thomas Münzel, Christian Weber, Esther Lutgens, Andreas Daiber, Steffen Daub

“…The present study investigates whether the CD40L-CD40-TRAF6 signaling pathway with focus on immune cells and adipocytes could be a therapeutic target in arterial hypertension.Arterial hypertension was induced in WT (C57BL6/J) and cell-specific CD40(L) knockout mice (AdipoqCre x CD40 fl/fl, CD4Cre x CD40 fl/fl, CD19Cre x CD40 fl/fl, and GP1baCre x CD40L fl/fl) via angiotensin (AT-II) infusion (1 mg/kg/d) for seven days. …”
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Outer retina micro-inflammation is driven by T cell responses prior to retinal degeneration in early age-related macular degeneration by Lucas Stürzbecher, Lucas Stürzbecher, Lucas Stürzbecher, Lucas Stürzbecher, Hendrik Bartolomaeus, Hendrik Bartolomaeus, Hendrik Bartolomaeus, Hendrik Bartolomaeus, Theda U. P. Bartolomaeus, Theda U. P. Bartolomaeus, Theda U. P. Bartolomaeus, Theda U. P. Bartolomaeus, Sylvia Bolz, Andjela Sekulic, Marius Ueffing, Marius Ueffing, Simon J. Clark, Simon J. Clark, Simon J. Clark, Nadine Reichhart, Sergio Crespo-Garcia, Nicola Wilck, Nicola Wilck, Nicola Wilck, Nicola Wilck, Olaf Strauß

“…Furthermore, flow cytometry and gene expression analysis were used on ocular and splenic tissues of Cx3cr1GFP/GFP and C57BL/6J mice at 8 and 12 months of age to characterize the dynamics of local and systemic T cell populations.ResultsWe show the presence of memory T cells such as CD45RO+ cells in the choroid and retina of patients with AMD with a peak of abundance in early stages of AMD. …”
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甘草素对去卵巢APP/PS1转基因小鼠肠道菌群的影响 by 耿清华, 赵月阳, 贺桂琼, 余华荣

“…术后第7天，将每组小鼠再分别用LG和生理盐水处理，另取10只野生型C57BL/6J小鼠作正常对照组（WT）。LG处理组剂量为30 mg•kg^-1•d^-1，药物处理90 d后，采集粪便样本，提取基因组，采用16S rDNA扩增子测序对各组小鼠的肠道菌群进行分析；利用水迷宫，评估小鼠的学习与记忆能力；采用免疫荧光观察小鼠脑内老年斑（SP）。…”
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Nanostructured lipid carriers based mRNA vaccine leads to a T cell–inflamed tumour microenvironment favourable for improving PD-1/PD-L1 blocking therapy and long-term immunity in a... by Carole Fournier, Marion Mercey-Ressejac, Valentin Derangère, Amal Al Kadi, David Rageot, Christine Charrat, Alexis Leroy, Julien Vollaire, Véronique Josserand, Marie Escudé, Séverine Escaich, François Ghiringhelli, Thomas Decaens, Fabrice P. Navarro, Evelyne Jouvin-Marche, Patrice N. Marche

“…We tested the anti-tumoral impact of Ova mRNA vaccine in B16-OVA and E.G7-OVA cold tumour-bearing C57Bl6 female mice as well as its synergy effect with an anti-PD-1 therapy by following the tumour growth and performing immunophenotyping of innate and adaptive immune cells. …”
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γδ T Cells’ Role in Donor-Specific Antibody Generation: Insights From Transplant Recipients and Experimental Models by Xavier Charmetant, Xavier Charmetant, Xavier Charmetant, Guillaume Rigault, Chien-Chia Chen, Hannah Kaminski, Hannah Kaminski, Jonathan Visentin, Jonathan Visentin, Benjamin Taton, Gabriel Marseres, Virginie Mathias, Alice Koenig, Alice Koenig, Alice Koenig, Thomas Barba, Thomas Barba, Thomas Barba, Pierre Merville, Pierre Merville, Stéphanie Graff-Dubois, Emmanuel Morelon, Emmanuel Morelon, Emmanuel Morelon, Julie Déchanet-Merville, Valérie Dubois, Jean-Paul Duong van Huyen, Lionel Couzi, Lionel Couzi, Olivier Thaunat, Olivier Thaunat, Olivier Thaunat

“…These findings were validated in vivo by the demonstration that following transplantation with an allogeneic Balb/c (H2d) heart, wild-type and TCRδKO C57BL/6 (H2b) mice developed similar DSA responses, whereas TCRαKO recipients did not develop DSA. …”
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Aspirin-triggered resolvin D1 modulates pulmonary and neurological inflammation in an IL-22 knock-out organic dust exposure mouse model by Alissa N. Threatt, Jade White, Jade White, Nathan Klepper, Nathan Klepper, Zachary Brier, Zachary Brier, Logan S. Dean, Logan S. Dean, Ash Ibarra, Macallister Harris, Kaylee Jones, Maëlis J. L. Wahl, Melea Barahona, Melea Barahona, Emmanuel O. Oyewole, Morgan Pauly, Julie A. Moreno, Julie A. Moreno, Tara M. Nordgren

“…We aimed to investigate the link between organic dust exposure and neuroinflammation, the role of IL-22 in the pulmonary and neurological immune response to organic dust exposure, and the immune-modulating therapeutic applications of AT-RvD1 in an IL-22 knock-out mouse model of organic dust exposure. C57BL/6J (WT) and IL-22 knock-out (KO) mice were repetitively exposed to aqueous agriculture organic dust extract (DE) 5 days per week for 3 weeks (15 total instillations) and treated with AT-RvD1 either once per week (3 total injections) or 5 times per week (15 total injections) for 3 weeks and allowed to recover for 3 days. …”
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Safety and efficacy of mesenchymal stromal cells mitochondria transplantation as a cell-free therapy for osteoarthritis by Ana Maria Vega-Letter, Cynthia García-Guerrero, Liliana Yantén-Fuentes, Carolina Pradenas, Yeimi Herrera-Luna, Eliana Lara-Barba, Felipe A. Bustamante-Barrientos, Masyelly Rojas, María Jesús Araya, Nicole Jeraldo, Constanza Aros, Francisca Troncoso, Daniela Poblete, Angela Court, Alexander Ortloff, Jose Barraza, Francesca Velarde, Carlos Farkas, Claudio Carril, Noymar Luque-Campos, Gonzalo Almarza, Maximiliano Barahona, Jose Matas, Lucas Cereceda, Rocío Lorca, Jorge Toledo, Karina Oyarce, Rolando Vernal, Andrés Caicedo, Andrea del Campo, Yessia Hidalgo, Roberto Elizondo-Vega, Farida Djouad, Maroun Khoury, Fernando E. Figueroa, Patricia Luz-Crawford

“…Furthermore, the transcriptional changes following mitochondrial uptake by chondrocytes were evaluated using an Affymetrix analysis, Lastly, the dose dependence therapeutic efficacy, biodistribution and immunogenicity of Mito-MSC were assessed in vivo, through an intra-articular injection in male C57BL6 mice in a collagenase-induced OA (CIOA) model. …”
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FGF21 ameliorates diabetic nephropathy through CDK1-dependently regulating the cell cycle by Yudie Zhang, Yudie Zhang, Yudie Zhang, Yudie Zhang, Yudie Zhang, Fan Wang, Fan Wang, Fan Wang, Fan Wang, Chongyang Zhang, Chongyang Zhang, Chongyang Zhang, Chongyang Zhang, Fan Yao, Fan Yao, Fan Yao, Fan Yao, Bin Zhang, Bin Zhang, Bin Zhang, Bin Zhang, Yongping Zhang, Xiaobo Sun, Xiaobo Sun, Xiaobo Sun, Xiaobo Sun

“…FGF21 has been shown to ameliorate diabetic nephropathy, and in addition FGF-21-treated mice impeded mitogenicity, whereas it is unclear whether FGF21 can influence DN progression by regulating the cell cycle in diabetic nephropathy.MethodsIn order to create a diabetic model, STZ injections were given to C57BL/6J mice for this investigation. Then, FGF21 was administered, and renal tissue examination and pathological observation were combined with an assessment of glomerular injury, inflammation, oxidative stress, and the fibrinogen system in mice following the administration of the intervention. …”
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A hepatocellular carcinoma model with and without parenchymal liver damage that integrates technical and pathophysiological advantages for therapy testing by Karina Benderski, Paul Schneider, Panayiotis Kordeves, Michael Fichter, Jenny Schunke, Federica De Lorenzi, Feyza Durak, Barbara Schrörs, Özlem Akilli, Fabian Kiessling, Matthias Bros, Mustafa Diken, Stephan Grabbe, Jörn M. Schattenberg, Twan Lammers, Alexandros Marios Sofias, Leonard Kaps

“…We developed an HCC model via intrasplenic injection of highly tumorigenic HCC cells, which, due to cellular tropism, invade the liver and allow for a controllable disease progression. Specifically, C57BL/6JRj mice were intrasplenically inoculated with Dt81Hepa1–6 HCC cells, with a subgroup pre-treated with CCl4 to induce cirrhosis (C-HCC). …”
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