Ginsenoside Rg1 alleviates chronic testicular damage caused by cranial irradiation through the SCF/PI3K/Akt/mTOR pathway in mice by Qiuhua Zhou, Yiquan Ou, Xiangsheng Tian, Yujun Ning, Yuwei Mao, Weichao Zhao, Dingxin Long

“…Objective: To investigate whether Ginsenoside Rg1 can mitigate the adverse effects of cranial irradiation on distal reproductive function in mice and to explore the underlying mechanisms. Methods: Forty male C57BL/6J mice were randomly divided to four groups [Control, irradiation (IR), IR ​+ ​Rg1, Rg1), IR ​+ ​Rg1 and Rg1 group treated with intraperitoneal injections of Ginsenoside Rg1 for 30 ​d, followed by single-dose irradiation of 5 ​Gy X-ray irradiation (2 ​Gy/min) for the IR and IR ​+ ​Rg1 group. …”
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Ginsenoside Rd protects against acute liver injury by regulating the autophagy NLRP3 inflammasome pathway by Xiaomei Zhong, Yibin Sun, Yanxiang Lin, Shan Deng, Huan Wang, Xian Zhou, Jinjian Lu, Yanfang Zheng, Ruoyin Luo, Mingqing Huang, Jianyuan Song

“…Forty-eight 8 weeks old C57BL/6 mice were treated with saline (control or model group), Rd (12.5 mg/kg, 25 mg/kg or 50 mg/kg), and diammonium glycyrrhizinate (DG, 30 mg/kg) for three days. …”
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Effects of Treadmill Exercise on Hippocampal Synaptic Plasticity and Expression of Related Proteins in Aging Mice by WANG Sinuo, CAO Yajun, XIANG Qing, YAN Jiamin, LIN Yanting, ZHUANG Yueyang, GUO Xiaoqin, LIU Weilin

“…ObjectiveTo explore the role and possible mechanism of cognitive function-related factors and BDNF/TrkB/PI3K signaling pathway in the treadmill exercise intervention with the decline of cognitive memory function in naturally aging mice.MethodsA total of 16 months old C57BL/6J mice were randomly divided into the aged group and the exercise group, through a random number table method, with 8 mice in each group. …”
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Mechanism of Inflammation Regulation of α7nAChR in Spleen and Spinal Dorsal Horn of Mice with Paclitaxel-induced Neuropathic Pain by Electroacupuncture on Zusanli Acupoint by PANG Lina, LAN Yanyan, WANG Zhifu, CHEN Xiaomei, YU Xiangmei

“…ObjectiveTo investigate the effect of electroacupuncture on Zusanli acupoint on the inflammatory changes of α7 nicotinic acetylcholine receptor (α7nAChR) in spleen and spinal dorsal horn of mice with paclitaxel-induced neuropathic pain (PINP) .MethodsA total of sixty adult male C57BL/6 mice with the body weight of 18-22 g were selected and randomly divided into control group, model group, electroacupuncture group, intraperitoneal antagonist group and intrathecal antagonist group according to random number table, with 12 mice in each group. …”
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Chemical Composition of Mexicali Propolis and Its Effect on Gastric Repair in an Indomethacin-Induced Gastric Injury Murine Model by Pilar Dominguez-Verano, Nadia Jacobo-Herrera, Andrés Castell-Rodríguez, Octavio Canales-Alvarez, Maria Margarita Canales-Martinez, Marco Aurelio Rodriguez-Monroy

“…Gastric injury was induced in male C57BL/6 mice by intragastric administration of indomethacin (10 mg/kg). …”
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激活TGR5缓解肾缺血再灌注后肾脏纤维化损伤 by 李蒙, 隆罗莎, 梁柏恩, 徐龙, 赵晓朵, 王蔚东, 李春凌

“…目的探讨激活胆汁酸受体TGR5在单肾缺血再灌注损伤合并对侧肾摘除（uIRIx）模型诱导的肾脏纤维化中的作用。方法体内实验：将C57BL/6J小鼠随机分成假手术（Sham）组、uIRIx组及uIRIx+石胆酸（LCA）组，每组6只，使用uIRIx模型诱导肾脏纤维化，通过血和尿生化指标评估肾脏功能，利用HE染色评估肾脏损伤程度，使用Masson染色及免疫组化对肾脏纤维化程度进行评估，并用Western Blotting检测肾脏皮质纤维化相关指标蛋白表达；分别在<italic>TGR5</italic>^+/+小鼠及<italic>TGR5</italic>^-/-小鼠中设置Sham组及uIRIx组，每组6只，利用Western Blotting检测各组肾脏纤维化程度。…”
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Unveiling the Mechanisms of a Remission in Major Depressive Disorder (MDD)-like Syndrome: The Role of Hippocampal Palmitoyltransferase Expression and Stress Susceptibility by Careen A. Schroeter, Anna Gorlova, Michael Sicker, Aleksei Umriukhin, Alisa Burova, Boris Shulgin, Sergey Morozov, Joao P. Costa-Nunes, Tatyana Strekalova

“…Here, we sought to investigate how susceptibility (sucrose preference below 65%) or resilience (sucrose preference > 65%) to stress-induced anhedonia affects DHHC gene expression in the hippocampus of C57BL/6J mice during the phase of spontaneous recovery from anhedonia. …”
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Qizhu Rougan Granules suppress liver fibrosis by inhibiting the expression of the P2Y14 receptor on hepatic stellate cells by Yujing Tao, Yujing Tao, Qun Niu, Qun Niu, Yuanqian Yao, Yuanqian Yao, Kaixin Wang, Kaixin Wang, Haijian Dong, Haijian Dong, Xin Zhao, Xin Zhao, Zijian Zeng, Zijian Zeng, Hui Li

“…In this study, we explored the effects and potential mechanisms of Qizhu-Ruogan-Granules (QZRG), a formulation from the Affiliated Hospital of the Chengdu University of TCM, on carbon tetrachloride (CCl4)-induced liver fibrosis in mice.MethodsA total of 40 male C57BL/6J mice were randomly divided into five groups (n = 8 per group), with liver fibrosis induced by injecting 10% CCl4 for 15 weeks. …”
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磷酸二酯酶5在肾脏纤维化中的保护作用 by 翟庆娴, 李蒙, 隆罗莎, 梁柏恩, 王蔚东

“…目的探究磷酸二酯酶5（PDE5）抑制剂西地那非（SIL）或LW1646对单侧输尿管结扎（UUO）诱导的肾间质纤维化的保护作用。方法C57BL/6雄性小鼠被随机分为假手术Sham（<italic>n</italic>=6）、7UUO（<italic>n</italic>=6）、7UUO+SIL（<italic>n</italic>=6）或7UUO+LW1646（<italic>n</italic>=6）组，术前1h给予SIL或LW1646、或溶剂灌胃给药，连续给药7 d后处死小鼠，收集梗阻侧肾脏标本。…”
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Shi-Zhen-An-Shen Decoction, a Herbal Medicine That Reverses Cuprizone-Induced Demyelination and Behavioral Deficits in Mice Independent of the Neuregulin-1 Pathway by Chao Ma, Yan Wu, Xinyao Liu, Yi He, Yuan Jia, Pei Chen, Dongqing Yin, Yanzhe Ning, Guoqiang Xing, Zuoli Sun, Hongxiao Jia

“…The aim of this study was to investigate the effect of SZASD on myelin in demyelinated mice exhibiting schizophrenia-like behaviors. Sixty male C57BL/6 mice were randomly divided into six groups (n=10 per group): (1) control group, (2) cuprizone (CPZ, a copper chelator that induced demyelination, 0.2% w/w)+saline, (3) CPZ+low-dose SZASD (8.65 g·kg-1·d-1), (4) CPZ+medium-dose SZASD (17.29 g·kg-1·d-1), (5) CPZ+high-dose SZASD (25.94 g·kg-1·d-1), and (6) CPZ+quetiapine (QTP, an atypical antipsychotic that served as a positive treatment control, 10 mg·kg-1·d-1). …”
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Tofogliflozin attenuates renal lipid deposition and inflammation via PPARα upregulation mediated by miR-21a impairment in diet-induced steatohepatitic mice by Sae Nishihara, Masahiro Koseki, Katsunao Tanaka, Takashi Omatsu, Ayami Saga, Hiroshi Sawabe, Hiroyasu Inui, Takeshi Okada, Tohru Ohama, Daisuke Okuzaki, Yoshihiro Kamada, Masafumi Ono, Makoto Nishida, Mikio Watanabe, Yasushi Sakata

“…Therefore, we used this model to evaluate the effects of tofogliflozin on renal lipid metabolism and inflammation. Male 8–10-week-old C57Bl/6 mice were fed a high-fat/high-cholesterol/high-sucrose/bile acid (HF/HC/HS/BA) diet with 0.015% tofogliflozin (Tofo group) or an HF/HC/HS/BA diet alone (SLD group). …”
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环状RNA circ_0120051通过miR-144-3p/IDH2轴抑制心肌成纤维细胞的纤维化表型研究 by 梁俣, 胡志琴, 温艺红, 伍华燕, 王娅, 刘宇鹏, 单志新, 方咸宏

“…RT-qPCR检测circ_0120051在人心肌细胞AC16中的核质分布情况。利用腺病毒在C57BL/6乳小鼠心肌成纤维细胞（mCFs）中过表达circ_0120051，通过RT-qPCR和Western blot检测过表达circ_0120051对mCFs中纤维化相关基因表达的影响，利用细胞划痕实验检测对mCFs迁移能力的影响。…”
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Therapeutic Potential of <i>Cajanus cajan</i> (L.) <i>Millsp</i>. Leaf Extract in Modulating Gut Microbiota and Immune Response for the Treatment of Inflammatory Bowel Disease by Mingzhang Lin, Linghua Piao, Zhendong Zhao, Li Liao, Dayong Wang, Haiwen Zhang, Xiande Liu

“…The IBD model was developed in C57BL/6 mice by administering continuous 4% (<i>w</i>/<i>v</i>) dextran sodium sulfate (DSS) aqueous solution over a period of seven days. …”
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Analysis of the spatiotemporal dynamics of vascular injury and regeneration following experimental Spinal Cord Injury by Christian J. Entenmann, Emily J. von Bronewski, Lilly Waldmann, Lea Meyer, Katharina Kersting, Laurens T. Roolfs, Lasse M. Schleker, Melina Nieminen-Kelhä, Irina Kremenetskaia, Frank L. Heppner, Michael G. Fehlings, Peter Vajkoczy, Vanessa Hubertus

“…Materials and methods: Adult C57BL/6J mice were subjected to clip-compression SCI (Th 6/7, 5g, 60s, n = 20) or sham injury (laminectomy, n = 4), and sacrificed at 1, 3, 7, 14, and 28 days (d) post-injury following intracardial fluorescein isothiocyanate (FITC)-Lectin perfusion. …”
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Repurposing the prostaglandin analogue treprostinil and the calcium-sensing receptor modulator cinacalcet to revive cord blood as an alternate source of hematopoietic stem and prog... by Michaela Prchal-Murphy, Julia Zehenter, Marlene Fischer, Anita Pirabe, Madeleine Themanns, Behnaz Afrashteh, Eva Maria Putz, Karoline Kollmann, José Basílio, José Basílio, Manuel Salzmann, Wolfgang Strohmaier, Günther Krumpl, Alex Farr, Veronika Sexl, Michael Freissmuth, Eva Zebedin-Brandl

“…Eight-week-old female and male C57BL/6J, BALB/c, or female NSG mice served as recipient models.ResultsWhen administered concomitantly, treprostinil and cinacalcet exhibited mutual antagonism: the survival of recipient animals was lower when both drugs were administered together compared to either agent alone. …”
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Arginase1敲低通过增强Aβ降解和减轻神经炎症改善APP/PS1小鼠认知功能 by 王清波, 唐姣玲, 郭开华, 徐杰

“…方法采用Arginase1基因工程小鼠，分为C57BL/6对照组（WT）、Arginase1低表达组（Arg1^+/-）、阿尔茨海默病小鼠模型组（APP/PS1）、Arginase1、APP/PS1转基因小鼠Arginase1低表达组（Arg1^+/-；APP/PS1），旷场实验（OFT）、Morris水迷宫（MWM）实验分别测试小鼠自主活动和适应能力、学习记忆能力，硫磺素S染色法和免疫荧光染色法检测小鼠脑内β-淀粉样蛋白（Aβ）沉积；小鼠海马区域Arginase1，小胶质细胞特征性标志物离子钙接头蛋白分子1（Iba1）、跨膜蛋白119（Tmem119）及溶酶体蛋白（CD68）表达量；ELISA法检测海马和皮质组织Aβ_1-40、Aβ_1-42表达量，蛋白免疫印迹（Western blot）法检测淀粉样蛋白前体蛋白 （APP）、APP-β位点剪切酶（BACE）、脑啡肽酶（NEP）、白细胞介素-1β（IL-1β）表达量。…”
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壮通饮通过Nrf2-SCL7A11/xCT-Gpx4通路调控铁死亡改善脑缺血再灌注损伤 by 王成毅, 蔡粤芳, 宁振求, 邓敏贞, 孙景波, Sookja Kim CHUNG, 李岩, 程骁

“…目的探究壮通饮通过Nrf2-SCL7A11/xCT-Gpx4通路调控铁死亡对大脑中动脉栓塞模型小鼠脑缺血再灌注损伤的保护作用及其机制。方法将C57BL/6J小鼠随机分为假手术组（Sham）、模型组（MCAO）、低剂量壮通饮组（ZTY-L+MCAO），高剂量壮通饮组（ZTY-H+MCAO），每组5只。…”
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Myocarditis and neutrophil-mediated vascular leakage but not cytokine storm associated with fatal murine leptospirosisResearch in context by Stylianos Papadopoulos, David Hardy, Frédérique Vernel-Pauillac, Magali Tichit, Ivo G. Boneca, Catherine Werts

“…Methods: To investigate the causes of death in lethal leptospirosis, we compared intraperitoneal infection of male and female C57BL6/J mice with 108 Leptospira of two strains of pathogenic L. interrogans. …”
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肌球蛋白重链7基因来源的miR-208b-3p促进心肌成纤维细胞中纤维化相关基因表达 by 张梦珍, 翟琳, 郭林林, 朱杰宁, 李晖, 徐金东, 方咸宏, 单志新

“…通过实时荧光定量PCR（RT-qPCR）检测miR-208b-3p 在血管紧张素Ⅱ（Ang Ⅱ）和高糖/葡萄糖氧化酶（G/Go）处理的C57BL/6乳小鼠心肌细胞（NMVCs）和心肌成纤维细胞（mCFs）中的表达；利用CCK8细胞增殖实验、流式细胞术和纤维化相关蛋白（包括COL1A1、COL3A1和α-SMA）表达检测来了解miR-208b-3p对mCFs纤维化表型的影响；双萤光素酶报告基因实验检测miR-208b-3p与潜在靶基因Mtf2和Pgrmc1 3'端非翻译区（3'-UTR）的结合作用；利用RT-qPCR和Western blot 法分别检测miR-208b-3p转染的 mCFs中Mtf2 和Pgrmc1 表达；通过小干扰RNA（siRNA）抑制Mtf2和Pgrmc1表达，并检测对mCFs中纤维化相关蛋白表达的作用。…”
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Interventions by Cardiovascular Drugs Against Aircraft Noise-Induced Cardiovascular Oxidative Stress and Damage by Marin Kuntić, Ivana Kuntić, Jiayin Zheng, Leonardo Nardi, Matthias Oelze, Arijan Valar, Dominika Mihaliková, Lea Strohm, Henning Ubbens, Qi Tang, Liyu Zhang, Guilherme Horta, Paul Stamm, Omar Hahad, Dilja Krueger-Burg, Huige Li, Sebastian Steven, Adrian Gericke, Michael J. Schmeisser, Thomas Münzel, Andreas Daiber

“…Using our established mouse exposure model, we applied mean sound pressure levels of 72 dB(A) for 4 d. C57BL/6 mice were treated with the beta-blocker propranolol (15 mg/kg/d s.c. for 5 d) or the alpha-blocker phenoxybenzamine (1.5 mg/kg/d s.c. for 5 d) and noise-exposed for the last 4 d of the drug administration. …”
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