The Upregulation of IL-1β Induced by Cisplatin Triggers PI3K/AKT/MMP9 Pathway in Pericytes Mediating the Leakage of the Blood Labyrinth Barrier by Yu M, Jiang WJ, Yu M, Zhou Z, Wang M, Li L

“…However, the relationship between CDDP-induced inflammation and BLB dysfunction remains elusive.Materials and Methods: In vivo and in vitro BLB models were used to explore the inflammatory mechanisms underlying CDDP ototoxicity. C57BL/6J mice were treated with CDDP and IL-1β levels, BLB permeability, and hearing thresholds were assessed using ELISA, histological staining, ABR test and BLB leakage tests. …”
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Enhancement of host defense against Helicobacter pylori infection through modulation of the gastrointestinal microenvironment by Lactiplantibacillus plantarum Lp05 by Yao Dong, Mei Han, Yongmei Qi, Ying Wu, Zhipeng Zhou, Dacheng Jiang, Zhonghui Gai

“…ObjectiveThis study aimed to assess the impact of Lactiplantibacillus plantarum Lp05 (Lp05) on the gastrointestinal microbiome and pathophysiological status of mice infected with Helicobacter pylori (H. pylori), exploring its potential as a probiotic treatment for H. pylori infections.MethodsIn vitro, the interaction between Lp05 and H. pylori was analyzed using laser confocal and scanning electron microscopy. In vivo, C57BL/6 mice infected with H. pylori were treated with Lp05 and divided into six groups: control, model, quadruple therapy, and three dosage levels of Lp05 (2×107, 2×108, 2×109 CFU/mouse/day). …”
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Recombinant α-Klotho Protein Alleviated Acute Cardiorenal Injury in a Mouse Model of Lipopolysaccharide-Induced Septic Cardiorenal Syndrome Type 5 by Xi Liu, Yangyang Niu, Xiaoqin Zhang, Yingying Zhang, Ying Yu, Jieli Huang, Jiangtao Li, Chen Yu

“…Methods. Male 8-week-old C57BL/6 mice were randomly assigned to the control group, lipopolysaccharide (LPS; 10 mg/kg) group, LPS (10 mg/kg)+α-klotho (0.01 mg/kg) group, and LPS (10 mg/kg)+α-klotho (0.02 mg/kg) group. …”
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استخراج، جداسازی و تعیین اثرات تنظیم کنندگی سیستم ایمنی پپتیدهای حلقوی مستخرج از گیاه Viola odorata by لادن دیانی, آزاده طاهری بروجنی, ژاله ورشوساز, مهدی علی عمرانی, مسعود صادقی دینانی, حسین هاشم پور

“…در ادامه تعداد 30 موش C57/Bl6 به صورت تصادفی در 6 گروه تقسیم شدند. گروه‌های مختلف دریافت‌کننده‌ی پپتیدهای حلقوی فراکسیون‌های 50 و 80 درصد در دوزهای mg/kg 5، 50 و 100 به صورت داخل صفاقی بودند. …”
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Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/... by Zhe Li, Jing Li, Lin Li, Qian Wang, Qian Zhang, Ling Tian, Chenchen Li

“…This study investigates the mechanism by which Klotho enhances the stability of atherosclerotic plaques in chronic kidney disease. apoE-/- knockout mice and C57BL/6 mice underwent 5/6 nephrectomy and then klotho-NC and klotho-mimic groups were set up to be fed a high-fat chow diet and a dummy group was created to be fed a normal chow diet. qPCR detected relative mRNA expression of klotho. …”
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MicroRNA-451a is a candidate biomarker and therapeutic target for major depressive disorder by Yan Chen, Yun Liu, Ming Xiao, Chun Wang, Panpan Hu, Qiuchen Cao, Hu Feng, Jingfan Xu, Weixi Feng, Huaiqing Sun, Huachen Ding, Junying Gao

“…Background Increasing evidence supports the role of microRNAs (miRNAs) in major depressive disorder (MDD), but the pathophysiological mechanism remains elusive.Aims To explore the mechanism of microRNA-451a (miR-451a) in the pathology and behaviours of depression.Methods Abnormal miRNAs such as miR-451a reported previously in the serum of patients with MDD were screened and then confirmed in a mouse model of depression induced by chronic restraint stress (CRS). Eight-week-old male C57BL/6 mice had miR-451a overexpression in the medial prefrontal cortex (mPFC) via adeno-associated virus serotype 9 vectors encoding a pri-mmu-miR-451a-GFP fusion protein followed by behavioural and pathological analyses. …”
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Microvascular dysfunction in a murine model of Alzheimer’s disease using intravital microscopy by Danielle Sidsworth, Noah Tregobov, Noah Tregobov, Colin Jamieson, Jennifer Reutens-Hernandez, Joshua Yoon, Geoffrey W. Payne, Geoffrey W. Payne, Stephanie L. Sellers, Stephanie L. Sellers, Stephanie L. Sellers, Stephanie L. Sellers

“…This pilot study used intravital microscopy to assess differences in responsiveness of gluteal muscle arterioles between a transgenic AD mouse model (APP/PS1; Tg) and wild-type (C57BL/6; WT) mice to further elucidate the role of vascular dysfunction in AD. …”
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伊伐布雷定抑制瑞芬太尼诱导的小鼠痛觉过敏 by 肖力, 王晓娥, 黄文起, 崔宇

“…目的探讨外周超极化激活环核苷酸门控（HCN）离子通道抑制剂伊伐布雷定（ivabradine）对瑞芬太尼诱导的痛觉过敏的作用。方法成年雄性C57/BL6小鼠尾静脉输注瑞芬太尼 2 μg/（kg·min）1 h建立痛觉过敏模型。在瑞芬太尼输注前30 min皮下注射伊伐布雷定（5 mg/kg），观察对瑞芬太尼诱导痛觉过敏的作用。…”
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Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology by Zhang KX, Zhang JW, Jiang YH, Wang YR, Liu ZL, Ding PL, Wang XY, Cui WQ, Xu XQ, Wang YH

“…Serum Pharmacology was used to analyze potential mechanisms of DSS for AD. C57BL/6J mice and Hippocampal neuronal cell line (HT-22) were used to prepare the AD model. …”
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FBR2 modulates ferroptosis via the SIRT3/p53 pathway to ameliorate pulmonary fibrosis by Yu Cheng, Yang Jiao, Wan Wei, Mengjia Kou, Yaodong Cai, Yang Li, Hao Li, Tonghua Liu

“…However, Feibi Recipe No. 2 (FBR2), a clinically validated traditional Chinese medicine formula, exhibits potential as an IPF treatment.ObjectiveThis study aimed to investigate the regulatory effect of FBR2 on ferroptosis through the SIRT3/p53 pathway and its therapeutic potential in improving IPF.MethodsPulmonary fibrosis was induced in C57BL/6J mice by intratracheal instillation of Bleomycin (BLM), followed by FBR2 treatment via gavage. …”
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Amygdalin Promotes Fracture Healing through TGF-β/Smad Signaling in Mesenchymal Stem Cells by Jun Ying, Qinwen Ge, Songfeng Hu, Cheng Luo, Fengyi Lu, Yikang Yu, Taotao Xu, Shuaijie Lv, Lei Zhang, Jie Shen, Di Chen, Peijian Tong, Luwei Xiao, Ju Li, Hongting Jin, Pinger Wang

“…In this study, tibial fractures in C57BL/6 mice were treated with amygdalin. Fracture calluses were then harvested and subjected to radiographic, histological, and biomechanical testing, as well as angiography and gene expression analyses to evaluate fracture healing. …”
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Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds by Jun Tan, Danshu Wang, Yu Lu, Yehan Wang, Zongcai Tu, Tao Yuan, Lu Zhang

“…The 40 % ethanol eluting fraction of 40EUS showed the strongest a-glucosidase inhibition with the IC50 value of 37.79 ng/mL, it could also effectively attenuate the fasting blood glucose level and oral glucose tolerance of C57BL/6 mice. Twenty-six compounds were identified from 40 % ethanol fraction by using HPLC-QTOF-MS/MS, hydrolysable tannins (including 11 ellagitannins and 4 gallotannins) were the major compounds, phenolic acids came to the second. …”
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CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies by Tomasz Kloskowski, Joanna Jarząbkowska, Arkadiusz Jundziłł, Daria Balcerczyk, Monika Buhl, Kamil Szeliski, Magdalena Bodnar, Andrzej Marszałek, Gerard Drewa, Tomasz Drewa, Marta Pokrywczyńska

“…The potential of CD133+ and CD133- cells for tumor induction was conducted on C57BL/6J mouse model. Three different cell quantities (100, 1000, 10000) were tested. …”
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Adverse outcome pathway-based assessment of pulmonary toxicity from the in vivo mixture of biocides dinotefuran and cetylpyridinium chloride by Yoon Cho, Mi-Kyung Song, Dong Im Kim, Min-Seok Kim, Kyuhong Lee

“…In this study, we aimed to validate these AOPs by assessing the toxicity of cetylpyridinium chloride and dinotefuran mixture. Sixty C57BL/6 male mice were exposed to either dinotefuran or cetylpyridinium chloride or a mixture of the two via intratracheal instillation (ITI) to examine the synergistic effects of MIE and key events (KEs) within putative AOPs. …”
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Neuroprotective Effects of Eugenol Acetate Against Ischemic Stroke by Chen L, Zhang R, Xiao J, Liang Y, Lan Z, Fan Y, Yu X, Xia S, Yang H, Bao X, Meng H, Xu Y, Yu L, Zhu X

“…For in vivo experiments, middle cerebral artery occlusion (MCAO) was constructed to mimic ischemic brain injury on 8-week-old male C57BL/6J mice. The mice were continuously injected intraperitoneally with EA or vehicle after MCAO. …”
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Establishment of a New Hyperglycemic Obesity Cardiac Dysfunction Mouse Model with Triacsin C by ZHAO Xiaona, WANG Peng, YE Maoqing, QU Xinkai

“…Objective This study aims to establish a novel hyperglycemic obesity mouse model by utilizing Triacsin C, an inhibitor of acyl-CoA synthetase long-chain family member 1 (ACSL1), combined with a high-fat diet, to simulate the changes in adipose tissue and cardiac function observed in patients with obesity-related type 2 diabetes.MethodsTwenty adult SPF-grade male C57BL/6J mice were randomly divided into two groups: the Control group (injected intraperitoneally with citric acid-sodium citrate buffer, Con group) and the TC group (injected intraperitoneally with Triacsin C, TC group). …”
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The regulatory role of cystatin C in autophagy and neurodegeneration by T. A. Korolenko, A. B. Shintyapina, A. B. Pupyshev, A. A. Akopyan, G. S. Russkikh, M. A. Dikovskaya, V. A. Vavilin, E. L Zavjalov, M. A. Tikhonova, T. G. Amstislavskaya

“…In elderly persons (61–80 years old compared to practically healthy people at 40–60 years of age), we revealed increased cystatin C levels both in serum and intraocular fluid. In an experiment on C57Bl/6J mice, cystatin C concentration was significantly higher in brain tissue than in the liver and spleen: an indication of an important function of this cysteine protease inhibitor in the brain. …”
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皮下注射巨噬细胞集落刺激因子诱导机械痛超敏的性别二态性 by 彭湘格, 林震嘉, 张辉, 冷世泽, 唐源, 郑钰凡, 谈智, 周利君

“…目的通过正常小鼠皮下注射巨噬细胞集落刺激因子（M-CSF）来研究其对疼痛行为的影响是否存在性别差异，并初步探讨其机制。方法将30只10周龄C57 BL/6J小鼠随机分为三组（每组10只，雌雄各半）：牛血清白蛋白对照组（BSA，0.3 μg）、低剂量M-CSF组（L M-CSF，0.075 μg）、高剂量M-CSF组（H M-CSF，0.3 μg）。…”
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Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon by Menglu Chen, Lei Gao, Pan Chen, Dandan Feng, Yalin Jiang, Yongchao Chang, Jianjun Jin, Fong-Fong Chu, Qiang Gao

“…Materials and Methods. C57BL/6J (B6) mice were treated with either low-dose (1.0 mg/kg) or high-dose (2.0 mg/kg) 5-HT by enema, low-dose (1.0%) or high-dose (2.5%) DSS, or combined low-dose (1.0%) DSS and (1.0 mg/kg) 5-HT. …”
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Mast Cell Degranulation Decreases Lipopolysaccharide-Induced Aortic Gene Expression and Systemic Levels of Interleukin-6 In Vivo by Jason M. Springer, Vineesh V. Raveendran, Mingcai Zhang, Ryan Funk, Donald D. Smith, Mehrdad Maz, Kottarappat N. Dileepan

“…Eight- to ten-week-old male C57BL/6 (wild-type) mice were injected with either (a) saline, (b) compound 48/80 (a systemic mast cell degranulating agent), (c) lipopolysaccharide (LPS), or (d) a combination of C48/80 and LPS. …”
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