氯胺酮对树突细胞体外分化和成熟的影响

“…【目的】 观察氯胺酮对树突细胞体外分化和成熟的影响?【方法】 取6 ~ 8周C57BL/6 野生型老鼠(雌雄不限)的骨髓细胞, 在FMS样酪氨酸激酶3(Flt3)配基的作用下诱导分化为树突细胞各种亚型?…”
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Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model by Hossein Rahavi, Seyed Mahmoud Hashemi, Masoud Soleimani, Jamal Mohammadi, Nader Tajik

“…Thus, we proposed in vitro assessment of adipose tissue-derived MSCs (AT-MSCs) immunomodulation on autoimmune response along with beta cell protection in streptozotocin- (STZ-) induced diabetic C57BL/6 mice model. MSCs were extracted from abdominal adipose tissue of normal mice and cultured to proliferate. …”
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AAV-KLF7 Promotes Descending Propriospinal Neuron Axonal Plasticity after Spinal Cord Injury by Wen-Yuan Li, Ying Wang, Feng-Guo Zhai, Ping Sun, Yong-Xia Cheng, Ling-Xiao Deng, Zhen-Yu Wang

“…In vivo, young adult C57BL/6 mice received a T10 contusion followed by an AAV-KLF7 injection at the T7–9 levels above the lesion. …”
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Differential Susceptibility to Particulate Matter-Induced Cardiac Remodeling and Senescence: A Comparative Study in Young and Aged Mice by Dunia Waked, Gabriel Henrique Rodella Guedes, Raissa Macedo, Paulo Hilário Nascimento Saldiva, Mariana Matera Veras, Ana Paula Cremasco Takano

“…Methods: Young and old male C57BL/6 mice were exposed to filtered or polluted air for six months using an ambient particle concentrator. …”
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Effect of Bacillus subtilis JZXJ-7, a Strain Isolated from Shrimp Paste, on Colonic Function and Microbial Community in Mice Infected with Vibrio parahaemolyticus by ZHOU Kui, WANG Rundong, GONG Mengxue, MOU Jingyuan, DENG Yijia, LI Xuepeng, ZHANG Yuhao, LI Jianrong

“…The protective effect of Bacillus subtilis JZXJ-7 (JZXJ-7) on Vibrio parahaemolyticus (Vp) infected mice was investigated. Totally 28 male C57BL/6J mice were randomly divided into four groups: a Vp infection model group (infected with the standard virulence strain ATCC33847), two JZXJ-7 intervention groups (administered at low (106 CFU) and high (108 CFU) doses by gavage), and a control group. …”
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Carrageenan-Induced Colonic Inflammation Is Reduced in Bcl10 Null Mice and Increased in IL-10-Deficient Mice by Sumit Bhattacharyya, Liquan Xue, Suzanne Devkota, Eugene Chang, Stephan Morris, Joanne K. Tobacman

“…Exposure to low concentrations of carrageenan (10 μg/mL in the water supply) has produced glucose intolerance, insulin resistance, and impaired insulin signaling in C57BL/6 mice. B-cell leukemia/lymphoma 10 (Bcl10) is a mediator of inflammatory signals from Toll-like receptor (TLR) 4 in myeloid and epithelial cells. …”
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Melatonin attenuates MPP+-induced autophagy via heat shock protein in the Parkinson’s disease mouse model by Yinli Guo, Chengbo Liu

“…Methods Twelve adult male C57BL/6 mice were randomly divided into four groups (normal control, melatonin control, Parkinson’s model, and melatonin treatment; n = 3 per group) and housed in a single cage. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was injected intraperitoneally in the Parkinson’s model and treatment groups to establish a subacute PD model, while controls received saline. …”
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Sevuparin strongly reduces hepcidin expression in cells, mice, and healthy human volunteers by Michela Asperti, Andrea Denardo, Magdalena Gryzik, Kristina E. M. Persson, Göran Westerberg, John Öhd, Maura Poli

“…These effects were evident in C57BL/6J mice after intravenous injection of a single dose of sevuparin (20 mg/kg) with a 70% reduction of hepcidin mRNA. …”
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Metabolic Context Regulates Distinct Hypothalamic Transcriptional Responses to Antiaging Interventions by Alexis M. Stranahan, Bronwen Martin, Wayne Chadwick, Sung-Soo Park, Liyun Wang, Kevin G. Becker, William H. WoodIII, Yongqing Zhang, Stuart Maudsley

“…In this paper, we investigated the effects of two well-characterized antiaging interventions, caloric restriction and voluntary wheel running, in two distinct physiological paradigms, that is, diabetic (db/db) and nondiabetic wild-type (C57/Bl/6) animals to investigate the contextual sensitivity of hypothalamic transcriptomic responses. …”
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Nobiletin restores the intestinal barrier of HFD-induced obese mice by promoting MHC-II expression and lipid metabolism by Ni Yang, Yue-Shan Pang, Yali Zheng, Yan-Ju Gong, Wei-Jun Ding

“…Our previous work demonstrated the therapeutic effect of nobiletin on obese mice; thus, we hypothesized that nobiletin could reverse HFD-induced damage to the intestinal barrier. Male C57BL/6 J mice were orally administered nobiletin for 14 d. …”
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颈总动脉压诱导的小鼠移植静脉血管重构

“…【目的】 研究血压升高引起血管重构的机制【方法】 手术取出麻醉后的11 ~ 12周供体C57BL/6J小鼠的下腔静脉，连接到同窝出生的受体小鼠颈总动脉，构建“静脉桥”继而,收获受颈总动脉压作用不同时间(0,2,4,8周)后的移植静脉,经石蜡包埋HE染色丽春红-维多利亚蓝染色以及平滑肌特异性抗体免疫组化染色后观察其血管构筑【结果】术后各时间点均可见移植静脉血管壁增厚及新生内膜的形成,呈时间依赖性与正常静脉[(11.10 ± 0.9)μm)]相比,术后8周血管壁[(88.97 ± 16)μm)]增厚了8倍(P < 0.01)术后2周移植静脉新生内膜中即可见血管平滑肌细胞大量增生，4周最多，8周后数量减少，但细胞外基质明显增多移植静脉在动脉压作用下，其弹性膜历经了断裂合成和重建过程【结论】 血压升高产生的机械力可直接导诱导移植的小鼠静脉粥样硬化以及弹力膜的重构本研究可望为深一步探索高血压引起血管重构的机制及防治新策略提供有用的动物模型及实验依据…”
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Obesity-Linked Gut Microbiome Dysbiosis Associated with Derangements in Gut Permeability and Intestinal Cellular Homeostasis Independent of Diet by Ravinder Nagpal, Tiffany M. Newman, Shaohua Wang, Shalini Jain, James F. Lovato, Hariom Yadav

“…We used leptin-deficient (Lepob/ob) mice in comparison to C57BL/6J control mice, which are fed on identical diets, and performed comparative and correlative analyses of gut microbiome composition, gut permeability, intestinal structural changes, tight junction-mucin formation, cellular turnover, and stemness genes. …”
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Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism, gut microbiota and microbial-associated metabolites by Ling Zhang, Wenyu Zou, Yongyan Hu, Honghua Wu, Ying Gao, Junqing Zhang, Jia Zheng

“…However, the mechanism with gut microbiota and fecal metabolites underlying the effects of voluntary wheel running on high-fat diet induced abnormal glucose metabolism has not been fully elaborated. C57BL/6 male mice were randomly assigned to 4 groups according to diets (fed with normal chow diet or high-fat diet) and running paradigm (housed in static cage or with voluntary running wheel). …”
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Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice by E. A. Litvinova, M. D. Belyaev, A. V. Prokhortchouk, V. S. Korostina, E. B. Prokhortchouk, E. N. Kozhevnikova

“…The survival of wild-type mice (C57BL/6J) during the treatment was 100 %. The weight of wild-type mice showed no decrease during the treatment. …”
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Deer antler velvet (Cervus elaphus sibiricus) promotes fracture healing via partial BMP2-Smad mediated osteoblast differentiation by Ji Hyang Gu, Min-Young Chae, Jeong June Choi, Thanh Chu Duc, Chang Gue Son, Eun-Jung Lee

“…The aim of this study investigated the bone-union properties of CES in a femoral fracture animal model and its corresponding molecular mechanisms. Methods Fifty-four C57BL/6 male mice underwent femoral shaft fracture by Bonnarens and Einhorn’s method, subsequently receiving a water extract of CES (200 mg/kg/day, daily) for 7 and 14 days. …”
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Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice by E. A. Litvinova, K. M. Achasova, M. A. Borisova, S. V. Zhenilo, E. B. Prokhortchouk, E. N. Kozhevnikova

“…In this study, we assessed the role of Kaiso in the development of intestinal inflammation using the experimental model of C57BL/6Muc2-/-Kaiso-/-. We have shown that mice with impaired intestinal barrier function that develop processes similar to human IBD also develop inflammatory responses, such as increased expression of Il1, Tnf and Il17a genes. …”
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Icaritin alleviates motor impairment and osteoporosis in Parkinson’s disease mice via the ER-PI3K/Akt pathway by Xianmei Lin, Xinyu Zhou, Xingman Liu, Lingqiong Xia, Jing Cai, Nanqu Huang, Yong Luo, Weidong Wu

“…Seventy-five 8-week-old C57BL/6J female mice underwent ovariectomy, followed by MPTP (20 mg/kg) injection for 7 days. …”
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Inflammatory Cytokine Pattern Is Sex-Dependent in Mouse Cutaneous Melanoma Experimental Model by Mihaela Surcel, Carolina Constantin, Constantin Caruntu, Sabina Zurac, Monica Neagu

“…Moreover, to test our experimental model, we have used low doses of dacarbazine (DTIC). C57 BL/6J mouse of both sexes were subjected to experimental cutaneous melanoma and treated with low doses of DTIC. …”
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Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice by Liang Dong, Lang Li

“…We have previously proved that in vitro large tumor suppressor kinase 2- (Lats2-) underexpressing BMSCs may enhance their tissue repair effects in ALI; thus, in the present study, we tried to explore whether Lats2-underexpressing BMSCs could rescue lipopolysaccharide- (LPS-) induced ALI in vivo. BMSCs from C57BL/6 mice transfected with Lats2-interfering lentivirus vector or lentivirus blank controls were transplanted intratracheally into LPS-induced ALI mice. …”
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Hemorrhagic Shock Induces a Rapid Transcriptomic Shift of the Immune Balance in Leukocytes after Experimental Multiple Injury by Lisa Debler, Annette Palmer, Sonja Braumüller, Bettina Klohs, Tom Eirik Mollnes, Karlheinz Holzmann, Markus Huber-Lang, Rebecca Halbgebauer

“…A well-established murine polytrauma (PT) model with or without an additional pressure-controlled HS (mean arterial pressure of 30 mmHg (±5 mmHg) for 60 mins, afterwards fluid resuscitation with balanced electrolyte solution four times the volume of blood drawn) was used. C57BL/6 mice were randomized into a control, PT, and PT + HS group with three animals in each group. …”
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