A H2S Donor GYY4137 Exacerbates Cisplatin-Induced Nephrotoxicity in Mice by Mi Liu, Zhanjun Jia, Ying Sun, Aihua Zhang, Tianxin Yang

“…Here we investigated the effect of GYY4137, a novel slow-releasing H2S donor, on cisplatin nephrotoxicity in mice. Male C57BL/6 mice were pretreated with GYY4137 for 72 h prior to cisplatin injection. …”
Get full text

Hyperhomocysteinemia-induced VCID results in visual deficits, reduced neuroinflammation and vascular alterations in the retina by Erica M. Weekman, Colin B. Rogers, Tiffany L. Sudduth, Donna M. Wilcock

“…Using our model of hyperhomocysteinemia-induced cSVD, we aimed to identify the effects of cSVD on visual sensitivity and cognition, retinal glial and vascular cells, and neuroinflammatory and cardiovascular gene expression changes. We placed C57Bl6/SJL mice on a HHcy-inducing diet, a model that has been well characterized to have vascular pathologies in the brain similar to pathologic cSVD. …”
Get full text

The efficacy of an allosteric modulator of the alpha 7 nicotinic acetylcholine receptor in a murine model of stroke by Katherine Hernandez, Nathan Jones, Sterling B. Ortega

“…Using a murine transient middle cerebral artery occlusion (tMCAO) model, we sought to evaluate the neurotherapeutic efficacy of a positive allosteric modulator of the alpha7 nicotinic acetylcholine receptor (α7-nAChR), PNU-120596 (PNU), specifically examining whether PNU would modulate stroke-induced neurological dysfunction and neuropathology, with modulation of neuroinflammation as a possible mechanism.MethodsYoung male C57BL/6J mice received a subcutaneous injection of 20mg/kg of vehicle (DMSO) or PNU-120596 immediately after reperfusion, and infarct area and Bederson score were analyzed 24 hours post-stroke. …”
Get full text

Spinal Involvement of TRPV1 and PI3K/AKT/mTOR Pathway During Chronic Postoperative Pain in Mice by Gabriela Xavier Santos, Tayllon dos Anjos-Garcia, Ana Carolina de Jesus Vieira, Giovane Galdino

“…Methods: In this study C57BL/6 male mice were used, and CPOP was induced by muscle retraction and incision. …”
Get full text

脾细胞输注致敏对异基因骨髓细胞损伤的机制

“…【目的】 探讨脾细胞输注致敏对异基因供者骨髓细胞损伤的作用机制65377;【方法】 应用异基因脾细胞输注的BALB/c小鼠作为致敏模型,未致敏(正常)BALB/c小鼠作为对照,异基因供者骨髓细胞来源于C57BL/6小鼠65377;通过补体依赖细胞毒性反应(CDC)65380;细胞毒性免疫细胞杀伤作用65380;抗体依赖细胞介导的细胞毒性作用(ADCC)65380;粒-单核细胞集落培养(CFU-GM)等实验研究致敏血清及致敏免疫细胞对异基因骨髓细胞的影响65377;【结果】 与补体孵育30 min或1 hr后,致敏血清组死亡细胞百分比明显高于正常血清组,其差异有统计学意义(P < 0.001);细胞毒性免疫细胞杀伤作用及ADCC实验中致敏组的细胞毒性指数均高于正常组,其差异亦有统计学意义(P < 0.05);致敏血清与骨髓细胞孵育组的CFU-GM数量比单纯骨髓细胞组有所减少,但两者差异无统计学意义(P > 0.05)65377;【结论】 致敏受者通过体液免疫及细胞免疫途径损伤异基因骨髓细胞65377;…”
Get full text

Protective Role of rAAV-NDI1, Serotype 5, in an Acute MPTP Mouse Parkinson's Model by Jennifer Barber-Singh, Byoung Boo Seo, Akemi Matsuno-Yagi, Takao Yagi

“…Using recombinant adenoassociated virus vectors (serotype 5) carrying our NDI1 gene, we were able to express the Ndi1 protein in the substantia nigra (SN) of C57BL/6 mice with an expression period of two months. …”
Get full text

Development of a conjunctival contact–type drug delivery device for latanoprost using hyaluronic acid by Soomin Lee, Mi-Young Jung, Choul Yong Park

“…We developed HA tablets loaded with latanoprost (HA-latanoprost) and verified the concentration using high-performance liquid chromatography. Twenty mice (C57BL6) were divided into four groups (n = 5 per group): normal saline (group 1), control HA tablet (group 2), Xalatan™ (group 3), and HA-latanoprost tablet (group 4). …”
Get full text

Nicotinamide adenine dinucleotide supplementation fails to enhance anesthetic recovery in rodents by Candida L. Goodnough, July Montoya, Erica B. Cartusciello, Erin L. Floranda, Eric R. Gross

“…Baseline and post-anesthesia (1.5% isoflurane) open field and Y-maze activity were recorded in adult male and female C57BL/6 mice (n = 8–10/group). NADH (150 mg/kg, intraperitoneal) or vehicle (0.9% normal saline) were given at baseline or during anesthesia. …”
Get full text

Influence of leptin administration to pregnant mice on fetal gene expression and adaptation to sweet and fatty food in adult offspring of different sexes by E. I. Denisova, E. N. Makarova

“…We aimed to investigate programming actions of maternal leptin on the signaling function of the placenta and fetal liver and on adaptation to high-calorie diet in male and female offspring. Female C57BL/6J mice received leptin injections in mid-pregnancy. …”
Get full text

Effects of Endurance Training and Chlorogenic Acid on Oxidative and Nitrosative Stress Markers in Prediabetic Male Mice by Sahar Ghasemi Pour, Sayyed M. Marandi

“…This study aimed to investigate the effects of endurance exercise and chlorogenic acid on oxidative and nitrosative stress indicators in skeletal muscle tissue of male C57BL/6 mice. Methods: A total of 35 mice were randomly divided into two groups: a high-fat diet group to induce pre-diabetes and a normal diet group. …”
Get full text

Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State by Marie Koren-Gluzer, Mira Rosenblat, Tony Hayek

“…Ex vivo studies were performed with murine peritoneal macrophages (MPM) harvested from control C57BL/6 and PON2-deficient (PON2KO) mice. PON2KO MPM showed an enhanced proinflammatory phenotype compared to the control, both in the basal state and following M1 activation by IFNγ and lipopolysaccharide (LPS). …”
Get full text

Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease by Yanan Sun, Ning Liu, Huihui Wang, Taiqi Qu, Fazheng Ren, Yixuan Li

“…This study aimed to clarify the effects of a high-fat diet (HFD) on allergy risk and the underlying mechanisms. Four-week-old male C57BL/6J mice were randomly divided into two groups and fed a normal fat diet (NFD) or HFD for 24 weeks. …”
Get full text

Imaging Collagen in Intact Viable Healthy and Atherosclerotic Arteries Using Fluorescently Labeled CNA35 and Two-Photon Laser Scanning Microscopy by Remco T.A. Megens, Mirjam G.A. oude Egbrink, Jack P.M. Cleutjens, Marijke J.E. Kuijpers, Paul H.M. Schiffers, Maarten Merkx, Dick W. Slaaf, Marc A.M.J. van Zandvoort

“…For in vivo labeling experiments, CNA35/OG488 was injected intravenously in C57BL6/J and apolipoprotein E −/− mice. Ex vivo CNA35/OG488 strongly labeled collagen in the tunica adventitia, media, and intima of muscular arteries. …”
Get full text

LPS-induced serum TNF production and lethality in mice: effect of L-carnitine and some acyl-derivatives by Vito Ruggiero, Claudio M. D'Urso, Claudio Albertoni, Silvia Campo, Piero Foresta, Edoardo Arrigoni Martelli

“…In this experimental model, C57BL/6 mice received 30 mg/kg LPS (E. cell 055:B5) injected intraperitoneally, while L-carnitine and its derivatives were administered according to different schedules. …”
Get full text

Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary by Ulises Urzua, Carlos Chacon, Renato Espinoza, Sebastián Martínez, Nicole Hernandez

“…We approached the age-parity relationship in the C57BL/6 mouse model and herein describe the presence of nonheme iron (hemosiderin) and deposits of the “age pigment” lipofuscin in reproductively aged mouse ovaries by applying conventional histochemical methods and autofluorescence. …”
Get full text

建立Duchenne 型肌营养不良鼠的骨髓移植模型 by 张为西, 张　成, 刘焯霖, 王　训, 刘晓蓉

“…【目的】建立Duchenne 型肌营养不良鼠(mdx 鼠)的骨髓移植模型, 摸索有效的放疗剂量。【方法】用体外培养的 不同数量的C57BL/6 雄鼠的骨髓细胞、骨髓基质细胞、骨髓悬浮细胞, 用不同方法移植给不同剂量放疗后的mdx 鼠, 观察受 体鼠的存活率及存活时间, 并对死亡鼠作GVHD(移植物抗宿主病)的病理学检测。…”
Get full text

Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide by Si-Ji Lv, Shu-Hui Hou, Lei Gan, Jing Sun

“…This study is aimed at establishing a lipopolysaccharide- (LPS-) induced primary ovarian insufficiency (POI) mouse model and investigating the underlying mechanism. C57BL/6N female mice were intraperitoneally injected with low-dose LPS (0.5 mg/kg) once daily for 14 days, high-dose LPS (2.5 mg/kg) twice weekly for 2 weeks, or cyclophosphamide (CTX; 150 mg/kg) once weekly for 2 weeks. …”
Get full text

Skeletal Muscle-Specific CPT1 Deficiency Elevates Lipotoxic Intermediates but Preserves Insulin Sensitivity by Wanchun Shi, Siping Hu, Wenhua Wang, Xiaohui Zhou, Wei Qiu

“…Mice with specific knockout of CPT1b in skeletal muscles (CPT1b M−/−) were used for the experiment group, with littermate C57BL/6 as controls (CPT1b). General and metabolic profiles were measured and compared between groups. mRNA expression and CPT1 activity were measured in skeletal muscle tissues and compared between groups. …”
Get full text

Diacylglycerol kinase alpha regulates post-hepatectomy liver regeneration by Hiroki Nakamoto, Shunsuke Shichi, Chisato Shirakawa, Takuto Suzuki, Hidemitsu Kitamura, Akinobu Taketomi

“…Here, we provide a detailed examination of C57BL/6 wild-type and DGKα knockout (KO) mice subjected to 70% partial hepatectomy (70% PH) modeling, including survival rates, hematological marker and gene expression levels, and histological analyses of factors related to liver regeneration. …”
Get full text

Toll-Like Receptor-4 Mediated Inflammation Is Involved in the Cardiometabolic Alterations Induced by Intermittent Hypoxia by Laureline Poulain, Vincent Richard, Patrick Lévy, Maurice Dematteis, Claire Arnaud

“…Lean adult male TLR4-deficient (TLR4−/−) mice and their controls (C57BL/6 mice) were exposed to either IH (FiO2 21-5%, 1 min cycle, 8 h/day) or air (normoxic mice) for 4 weeks. …”
Get full text