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    人纤溶酶原 K5 突变体Ⅰ的构建及其在大肠杆菌中的表达与纯化 by 蔡卫斌

    Published 2004-01-01
    “…【目的】 构建人纤溶酶原Kringle 5(K5)的突变体Cys461-Cys540(K5 mut1),在大肠杆菌中表达,亲和层析纯化,为探讨K5抗血管增生活性与Kringle结构域的关系提供基础?【方法】 以K5全长cDNA为模板用PCR的方法扩增人纤溶酶原K5 mut1基因,将其克隆进表达载体pET-22b(+)中,并用限制性核酸内切酶酶切和DNA测序鉴定其连接正确;pET-22b(+)/K5 mut1 转化大肠杆菌BL21(DE3), IPTG诱导表达,表达产物用固化Ni2+-His Bind Resin亲和层析方法纯化,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western blot方法分析鉴定?…”
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  5. 262165

    Theoretical impact of a bedside decision-making tool on antibiotic use for suspected neonatal healthcare-associated infection: an observational study by Lizel Georgi Lloyd, Mirjam Maria van Weissenbruch, Adrie Bekker, Cecilia Ferreyra, Birgitta Gleeson, Angela Dramowski

    Published 2025-01-01
    “…The antibiotic decision-making tool included an infection prediction score (NeoHoP), and a point-of-care C-reactive protein test (CRP) performed at HAI diagnosis and 24 h later. …”
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