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人纤溶酶原 K5 突变体Ⅰ的构建及其在大肠杆菌中的表达与纯化
Published 2004-01-01“…【目的】 构建人纤溶酶原Kringle 5(K5)的突变体Cys461-Cys540(K5 mut1),在大肠杆菌中表达,亲和层析纯化,为探讨K5抗血管增生活性与Kringle结构域的关系提供基础?【方法】 以K5全长cDNA为模板用PCR的方法扩增人纤溶酶原K5 mut1基因,将其克隆进表达载体pET-22b(+)中,并用限制性核酸内切酶酶切和DNA测序鉴定其连接正确;pET-22b(+)/K5 mut1 转化大肠杆菌BL21(DE3), IPTG诱导表达,表达产物用固化Ni2+-His Bind Resin亲和层析方法纯化,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western blot方法分析鉴定?…”
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A global analysis of dairy consumption and incident cardiovascular disease
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Theoretical impact of a bedside decision-making tool on antibiotic use for suspected neonatal healthcare-associated infection: an observational study
Published 2025-01-01“…The antibiotic decision-making tool included an infection prediction score (NeoHoP), and a point-of-care C-reactive protein test (CRP) performed at HAI diagnosis and 24 h later. …”
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Improving Road Semantic Segmentation Using Generative Adversarial Network
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A meta-analysis of the impact of TOE adoption on smart agriculture SMEs performance.
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Праектаванне беларуска- і рускамоўных натуральна-маўленчых інтэрфейсаў для даведкавых сістэм
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Habitat Quality Dynamics in Urumqi over the Last Two Decades: Evidence of Land Use and Land Cover Changes
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Synergetic role of TRPV4 inhibitor and mechanical loading on reducing inflammation
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Fast Hadamard-Encoded 7T Spectroscopic Imaging of Human Brain
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Diferencias entre la sensibilidad paterna según variables sociodemográficas
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