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    Isolation of Mesophyll Protoplasts from Leaves of <i>Dalbergia sissoo</i> Roxb by I Mukhtar, R Bajwa, G Nasim

    Published 2013-07-01
    “…The optimum combination of enzymes for protoplast isolation was 1.5% cellulase R-10+ 0.5 % pectinase R-1 after incubation for 6 hr at 28o C. The isolated protoplasts were round and filled with chloroplasts. …”
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